• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.147-150
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• 2000

Bioprocess chromatography is probably the most widely used unit operation in biopharmaceutics manufacturing. To obtain a good quality product reproducibly, the chromatography process validation should be performed carefully. We developed a miniaturized automatic chromatography system, MiniValChrom, for column performance validation.

• Journal of Bio-Environment Control
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• v.1 no.2
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• pp.175-180
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• 1992

Cherry tomatoes(Lycopersicon esculentum) were grown in circulating deep flow hydroponic system. The pH of the nutrient solution was increased and the EC was decreased with time. The clusters of ‘Mini Carol’ were branched severely, whereas those of ‘Chelsea Mini’ and ‘Popo’ were a little branched or not. The vegetative growth was similar between cultivars. But the yield of fruits was by far the largest in ‘Mini Carol’ compared to other cultivars, and not different between ‘Chelsea Mini’ and ‘Popo’. Internal qualities of fruits, like as sugar, sugar/organic acid, and vitamin C, are good in ‘Mini Carol’. As the result of panel test, ‘Mini Carol’ is good for softness, easeness of eating, toughness of skin and so on, so it is believed that ‘Mini Carol’ is the most acceptable to consumers in hydroponic culture.

• KSBB Journal
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• v.17 no.4
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• pp.326-332
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• 2002

The quality of biopharmaceutical proteins is strongly affected by a manufacturing process employed to produce Et, and thus validation of the manufacturing bioprocess is a very important issue. Chromatography is probably the most widely used bioprocess unit operation for protein purification. In this study, a miniaturized automatic chromatography system was designed and constructed for scale-down studies for process chromatography validation. This system, named MiniValChrom, has the following features: automatic and repeated operation, flexible sequences and intervals among the steps, on-line and real-time monitoring and control, method files savings, etc. Using the MiniValChrom, we peformed a case study of an abbreviated experiment to estimate chromatographic resin lifetime. BSA (bovine serum albumin) and Cibacron Blue 3G-A were used as the model protein and the resin, respectively. The resin deterioration was evaluated by determining and monitoring the HETP and NTP values from the chromatograms every 5 cycles. It was observed that the HETP and the NTP values were changed by 9% after 15 cycles. The resin lifetime validation could be completed by repeating this experiment until the HETP value reached a predetermined value. The MiniValChrom's concept and the protocol suggested in this study can serve as a rapid and economical tool for the validation studies of bioprocess chromatography system.

• Proceedings of the Korea Information Processing Society Conference
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• 2012.11a
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• pp.27-28
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• 2012

오늘날 차량 기술에 대한 관심사는 기계적 성능에서 각종 전자장치를 사용한 기술로 이동하고 있다. 특히 자동차 내에서 각종 멀티미디어 서비스 및 여러 정보들의 통합 분석을 위해서 자동차 네트워크 기술이 각광받고 있다. 본 논문에서는 자동차 네트워크 기술 중 MOST(Media Oriented System Transport)네트워크를 사용하기 위한 장치로 저가형 장치인 MiniAMG 단말기를 제안한다. Mini AMG 단말기를 통해 어떻게 오디오 스트리밍 서비스를 제공하는지와 향후 어떠한 방향으로 사용 가능한지에 대해 기술하였다.

• Fire Science and Engineering
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• v.23 no.5
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• pp.84-89
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• 2009

In this study, the fire extinguishing experiment was performed using a water mist nozzle with variation of factors which affect on the extinguishing time. The variables were distance from nozzle center to fire location, droplet size, height of nozzle and opening or not. With the experimental data, interaction and sensitivity between factors were analysed with Mini tab and deduce a optimum model of fire extinguishing of water mist system. Based on the experiment and modeling of fire extinguishing with water mist system, the most important factor on extinguishing time is the distance from the center of nozzle to fire and the opening effect was small compare with other factors.

• Reproductive and Developmental Biology
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• v.36 no.3
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• pp.207-212
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• 2012

The shortage of human organs for transplantation has induced the research on the possibility of using animal as porcine. However, pig to human transplantation as known as xeno-transplantation has major problem as immunorejection. Recently, the solutions of pig to human xenotransplantation are commonly mentioned as having a genetically modification which include alpha 1, 3 galatosyl transferase knockout (GTKO) and immune-suppressing gene transgenic model. Unfortunately, the expression level of transgenic gene is very low activity. Therefore, development of gene overexpression system is the most urgent issue. Also, the tissue specific overexpression system is very important. Because most blood vessels are endothelial cells, establishment of the endothelial-specific promoter is attractive candidates for the introduction of suppressing immunorejection. In this study, we focus the ICAM2 promoter which has endothelial-specific regulatory region. To detect the regulatory region of ICAM2 promoter, we cloned 3.7 kb size mini-pig ICAM2 promoter. We conduct serial deletion of 5' flanking region of mini-pig ICAM2 promoter then selected promoter size as 1 kb, 1.5 kb, 2 kb, 2.5 kb, and 3 kb. To analyze promoter activity, luciferase assay system was conducted among these vectors and compare endothelial activity with epithelial cells. The reporter gene assay revealed that ICAM2 promoter has critical activity in endothelial cells (CPAE) and 1 kb size of ICAM2 promoter activity was significantly increased. Taken together, our studies suggest that mini-pig ICMA2 promoter is endothelial cell specific overexpression promoter and among above all size of promoters, 1 kb size promoter is optimal candidate to overcome the vascular immunorejection in pig to human xenotransplantation.

• Journal of the Earthquake Engineering Society of Korea
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• v.14 no.6
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• pp.33-43
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• 2010

This paper deals with the hybrid structural test technique which has been introduced and studied currently in Korea. In this study, a Mini-MOST system which was developed as a part of NEES research was modified and improved to reduce the total simulation time to half of the original system. Using the proposed system together with the 2 dimensional small steel frame specimen, the validity and efficiency of the hybrid test technique is investigated. Even though the hybrid test has been developed as an alternative to the shaking table test and has been studied and applied for a long time in several countries, no attempt has been made to compare it directly with the shaking table test. Therefore, in this study, the hybrid test results are compared with those of the shaking table test as well as with a numerical simulation for the verification of hybrid test. From the comparison and analysis of the test results, it is concluded that the hybrid test can simulate the actual seismic behavior of structural systems very accurately and it can be a good alternative to the shaking table test.

• Journal of the Korea Institute of Military Science and Technology
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• v.18 no.4
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• pp.395-401
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• 2015

A mini ball launch system was developed for the study of dynamic fracture of ceramic materials. The principle of velocity multiplication system is similar to two stage gun. The plastic sabot assembly houses steel plunger and the void filled with silicone rubber. The sabot is stopped by the stopper block then the steel plunger inside the sabot compress the silicone rubber to high pressure. Then the compressive energy of the silicone rubber is transferred to the ball. More than ten times of initial speed was attained. In this study, most effective silicone rubber for the highest final speed was chosen out of three different varieties by launch tests.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.7
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• pp.3714-3732
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• 2019

With the rapid development of network, Intrusion Detection System(IDS) plays a more and more important role in network applications. Many data mining algorithms are used to build IDS. However, due to the advent of big data era, massive data are generated. When dealing with large-scale data sets, most data mining algorithms suffer from a high computational burden which makes IDS much less efficient. To build an efficient IDS over big data, we propose a classification algorithm based on data clustering and data reduction. In the training stage, the training data are divided into clusters with similar size by Mini Batch K-Means algorithm, meanwhile, the center of each cluster is used as its index. Then, we select representative instances for each cluster to perform the task of data reduction and use the clusters that consist of representative instances to build a K-Nearest Neighbor(KNN) detection model. In the detection stage, we sort clusters according to the distances between the test sample and cluster indexes, and obtain k nearest clusters where we find k nearest neighbors. Experimental results show that searching neighbors by cluster indexes reduces the computational complexity significantly, and classification with reduced data of representative instances not only improves the efficiency, but also maintains high accuracy.

• Dementia and Neurocognitive Disorders
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• v.17 no.3
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• pp.100-109
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• 2018

Background and Purpose: Although the clock drawing test (CDT) is a widely used cognitive screening instrument, there have been inconsistent findings regarding its utility with various scoring systems in patients with mild cognitive impairment (MCI) or dementia. The present study aimed to identify whether patients with MCI or dementia exhibited impairment on the CDT using three different scoring systems, and to determine which scoring system is more useful for detecting MCI and mild dementia. Methods: Patients with amnestic mild cognitive impairment (aMCI), vascular mild cognitive impairment (VaMCI), mild Alzheimer's disease (AD), mild vascular dementia (VaD), and cognitively normal older adults (CN) were included. All participants were administered the CDT, the Korean-Mini Mental State Examination (K-MMSE), and the Clinical Dementia Rating scale. The CDT was scored using the 3-, 5-, and 15-point scoring systems. Results: On all three scoring systems, all patient groups demonstrated significantly lower scores than the CN. However, while there were no significant differences among patients with aMCI, VaMCI, and AD, those with VaD exhibited the lowest scores. Area under the Receiver Operating Characteristic curves revealed that the three CDT scoring systems were comparable with the K-MMSE in differentiating aMCI, VaMCI, and VaD from CN. In differentiating AD from CN, however, the CDT using the 15-point scoring system demonstrated the most comparable discriminability with K-MMSE. Conclusions: The results demonstrated that the CDT is a useful cognitive screening tool that is comparable with the Mini-Mental State Examination, and that simple CDT scoring systems are sufficient for differentiating patients with MCI and mild dementia from CN.