• 한국토양비료학회지
• /
• 제46권5호
• /
• pp.351-358
• /
• 2013

A suitable supply of mineral elements into shoot via a root system from growth media makes plants favorable growth and yield. The shortage or surplus of minerals directly affects overall physiological reactions to plants and, especially, strongly influences carbohydrate metabolism as a primary response. We have studied mineral uptake and synthesis and translocation of soluble carbohydrates in N, P or K-deficient tomato plants, and examined the interaction between soluble carbohydrates and mineral elements. Four-weeks-old tomato plants were grown in a hydroponic growth container adjusted with suboptimal N ($0.5mmol\;L^{-1}\;Ca(NO_3)2{\cdot}4H_2O$ and $0.5mmol\;L^{-1}\;KNO_3$), P ($0.05mmol\;L^{-1}\;KH_2PO_4$), and K ($0.5mmol\;L^{-1}\;KNO_3$) for 30 days. The deficiency of specific mineral element led to a significant decrease in its concentration and affected the concentration of other elements with increasing treatment period. The appearance of the reduction, however, differed slightly between elements. The ratios of N uptake of each treatment to that in NPK sufficient tomato shoots were 4 (N deficient), 50 (P deficient), and 50% (K deficient). The P uptake ratios were 21 (N deficient), 19 (P deficient), and 28% (K deficient) and K uptake ratios were 11 (N deficient), 46 (P deficient), and 7% (K deficient). The deficiency of mineral elements also influenced on carbohydrate metabolism; soluble sugar and starch was substantially enhanced, especially in N or K deficiency. In conclusion, mineral deficiency leads to an adverse carbohydrate metabolism such as immoderate accumulation and restricted translocation as well as reduced mineral uptake and thus results in the reduced plant growth.

• 한국토양비료학회지
• /
• 제48권6호
• /
• pp.683-688
• /
• 2015

Nowadays, sustainable and environment-friendly agriculture has become an important issue all around the world, and repeated applications of mineral and/or organic fertilizer will probably affect mineral nutrient dynamics in soil in the long term but only a limited number of observations are available. This study was carried out to investigate whether there is any influence of different fertilizer management for red pepper (Capsicum annuum L.) cultivation on soil physicochemical properties, leaf mineral content, yield and fruit quality in the aspect of long-term practice in open field condition. NPK, NPK+compost, compost only, and unfertilized control plot were included in the treatments. The application of chemical fertilizer and/or compost repeated annually for 17 years from 1994 to 2011. Soil organic matter content was higher in compost treatments than in no-manure treatments. Available phosphate and the yield of red pepper were highest in NPK+compost treatment followed by NPK (chemical fertilizer), compost, and control. The results indicate that in the long term, nitrogen supply is still needed for increasing red pepper yield, but reduction in the use of chemical fertilizer could be also possible with the proper application of compost.

• 한국초지조사료학회지
• /
• 제21권4호
• /
• pp.191-202
• /
• 2001

Experiments were conducted on established grassland sward at Gongiam, Kwangju, and Kyung-gi in Korea from 1995 to 1997. The influence of mineral-N fertilizer or animal manure(AW) on herbage dry matter(DM) yield, N yield, the recovery of AM-N, and soil N and organic matter(0M) content in the mixed sward('potomac' orchardgrass, 'fawn' tall fescue, and 'kenblue' Kentuky bluegrass) was investigated. The treatments were replicated three times in a split plot block design. AM(the main plots) was applied at 200kg N ha ' year ' on each plot. The types of AM were cattle feedlot manure(CFM), pig manure fermented with sawdust(PMFS) and Korea native cattle slurry(KNCS). Three levels of mineral-N fertilizer, as urea, ranging from 0 to 200kg N $ha^{-1}\;year^{-1}$ in 100kg increments, were superimposed on each plot. The fertilizers and AM were applied in two equal dressings(the end of March and middle of November). AM and mineral fertilizer had significant effects(p<0.05) on herbage DM and N yields. Herbage yields in KNCS were higher than those in CFM and PMFS(p

• 한국토양비료학회지
• /
• 제48권4호
• /
• pp.305-311
• /
• 2015

Both low and high temperatures affect plant growth and development at whole plant level, tissue and even cell level through a variety of metabolic changes. Temperature stress is one of frequently occurring problems in greenhouse crops in summer and winter seasons due to the wide-spread year-round cultivation. In the present study, we investigated the extent of the inhibition of growth, macro-element uptake and soluble carbohydrate production, and the effect of extra-supply of minerals as a means of the recovery from temperature damage. Tomato plants were grown five different growth temperatures (15/8, 20/13, 28/21, 33/23 and $36/26^{\circ}C$), and extra-supply of minerals was composed of 1.5- and 2.0-fold stronger than the standard nutrition (1/2 strength of Hoagland's solution). Temperature stress significantly adversely affected tomato growth and mineral uptake, whereas soluble carbohydrate accumulation represented temperature-dependent response, more accumulation at low temperature and more consumption at high temperature. The soluble sugars in leaves and stems were mostly declined with the supply of extra-minerals at low and optimal temperatures, whereas remained unchanged at high temperature. The starch levels also remained unchanged or slightly decreased.

• 한국초지조사료학회지
• /
• 제14권3호
• /
• pp.223-229
• /
• 1994

To find out the effects of phosphatic fertilizer application levels on the botanical composition, mineral contents and mineral nutrient deprivation of grasses and the chemical properties of soil, this expriment was arranged as a randomized complete block design with six treatments(0, 50, 100, 150, 200 and 250kg $P_2O_5$/ha) and conducted at hilly land in Gwangju, Gyeonggi Province from September, 1989 to October, 1992. The results obtained are summarized as follows: The botanical composition of grasses without posphatic fertilizer application was poor but with 150kg $P_2O_5$/ha application, remarkably increased in legume of grasses and decreaed in weed. As the phophatic fertilizer levels increase, P, Ca and Mg content of grasses were increased but N and K content tended not to be regular, and Ca/p and K/Ca+ Mg equivalent ratio were lowered. Up to 150kg $P_2O_5$/ha application level, the mineral nutrient content deprived by dry matter yield was increased but with that over application, tend not to be increased. Most of applied mineral elements were accumulated at a subsurface of grassland and as the phosphatic fertilizer application levels increase, the available $P_2O_5$ and exchangeable Mg contents of the soil tended to be remarkably increased and exchangeable K content tended to be decreased, but pH, OM and exchangeable Ca content tended not to be regular.

• 한국토양비료학회지
• /
• 제50권2호
• /
• pp.93-99
• /
• 2017

Analysis of mineral nutrients in plant is required for evaluating diagnosis of plant nutritional status. Pretreatment procedure for the analysis of plant can be varied depending on elements to be analyzed. Wet-digestion is suitable for total nitrogen, phosphate and cations, however, digestion solution including nitric acid is not suitable for nitrogen analysis. Incineration procedure is required to analyze chloride, silicate and total sulfur. After digestion, total nitrogen is analyzed by Kjeldahl method, and phosphate is detected at 470nm by colorimetric analysis with ammonium meta vanadate. Cations and micro elements are determined by titration or colorimetry, also, these elements can be measured by Atomic absorption spectrometer (AAS) or Inductively coupled plasma spectrometer (ICP).

• 한국토양비료학회지
• /
• 제47권6호
• /
• pp.563-570
• /
• 2014

The shortage or surplus of minerals directly affects overall physiological metabolism of plants; especially, it strongly influences carbohydrate metabolism as a primary response. We have studied mineral uptake, synthesis and partitioning of soluble carbohydrates, and the relationship between them in N, P or K-excessive tomato plants, and examined the interaction between soluble carbohydrates and mineral elements. Four-weeks-old tomato plants were grown in a hydroponic growth container adjusted with excessive N ($20.0mmol\;L^{-1}$ $Ca(NO_3)2{\cdot}4H_2O$ and $20.0mmol\;L^{-1}$ $KNO_3$), P ($2.0mmol\;L^{-1}$ $KH_2PO_4$), and K ($20.0mmol\;L^{-1}$ $KNO_3$), respectively, for 30 days. Shoot growth rates were significantly influenced by excessive N or K, but not by excessive P. The concentrations of water soluble N (nitrate and ammonium), P and K were clearly different with each tissue of tomato plants as well as the mineral conditions. The NPK accumulation in all treatments was as follows; fully expanded leaves (48%) > stem (19%) = roots (16%) = petioles (15%) > emerging leaves (1). K-excessive condition extremely contributed to a remarkable increase in the ratio, which ranged from 2.79 to 10.34, and particularly potassium was dominantly accumulated in petioles, stem and roots. Fresh weight-based soluble sugar concentration was the greatest in NPK-sufficient condition ($154.8mg\;g^{-1}$) and followed by K-excessive (141.6), N-excessive (129.2) and P-excessive (127.7); whereas starch was the highest in K-excessive ($167.0mg\;g^{-1}$) and followed by P-excessive (146.1), NPK-sufficient (138.2) and N-excessive (109.7). Soluble sugar showed positive correlation with dry weight-based total N content (p<0.01) whereas was negatively correlated with soluble P (p<0.01) and dry weight-based total P (p<0.01). On the other hand, starch production was negatively influenced by total N (p<0.001), but, it showed positive relation with total K concentration (p<0.05). This study shows that uptake pattern of NPK and production and partitioning of soluble carbohydrate were substantially different from each mineral, and the relationship between water soluble- and dry weight-based-mineral was positive.

• 한국초지조사료학회지
• /
• 제11권3호
• /
• pp.182-188
• /
• 1991

With a purpose of finding out the effects of cattle slurry applicatioin on the dry matter yield, yield component, and botanical composition in the mixed sward, a field experiment was conducted with two times and five levels of cattle slurry application. The experimental field was laid.out in a split-plot design with three replications, and lasted from September. 1986 to October, 1990 at the Livestock Experiment Station in Suweon, The results obtained are summarized as follows: 1. At each harvest time, the plant growth of the plots with cattle slurry application was slightly worse when compared to dressing of mineral fertilizer. 2. Average dry matter yield for 4 years was not significantly different between the slurry application times, just after cutting, and 15 days after cutting, The DM yield in the plot with cattle slurry 30 m!/ha and mineral fertilizer(9,095 kg/ha) and with cattle slurry 60 m1/ha and mineral fertilizer(8, 987 kg/ha) were decreased by 8% and 10% than that of the plot with mineral fertilizer application(9,937 kg/ha), respectively. 3. The DM yield component of the plots with cattle slurry application just after cutting was much better when compared to manuring 15 days after cutting. And it was much better in the plot with cattle slurry 60 m"/ha and mineral fertilizer which was composed of 73.8% grasses(6.636 kg/ha). 24.6% legumes (2,207 kdha) and 1.6% weeds(144 kdha). 4. In general, the botanical composition was relatively good in the plots with slurry application just after cutting. Grassland vegetation of the plots with cattle slurry 60 m"/ha and mineral fertilizer was changed into much better botanical composition with 66.8% grasses, 30.2% legumes and 3. 1% weeds at the end of the experiment.xperiment.

• 한국토양비료학회지
• /
• 제49권6호
• /
• pp.689-698
• /
• 2016

Specific metabolic network responses to mineral starvation are not well-defined. We examined a detailed broad-scale identification of metabolic responses of tomato leaf and root to N, P or K starvation. Tomato plants were grown hydroponically under optimal (5 mM N, 0.5 mM P, or 5 mM K) and starved (0.5 mM N, 0.05 mM P, or 0.5 mM K) conditions and metabolites were measured by LC-MS and GC-MS. Overall, the levels of metabolites (lipids, nucleotides, peptides and secondary metabolites) presented in this paper largely showed mineral- and tissue-specific responses. Most strikingly, G3P (glycerol-3-P), GPC (glycerol-P-choline) and choline phosphate responded differently to a type of mineral; an increase in N or K starvation and a decrease in P starvation. A dramatic increase in the levels of secondary metabolites, in particular, rutin and chlorogenate in both tomato tissues during N starvation were observed. Based on these data, it is necessary to clearly elucidate an unknown event taking place in a variety of abiotic impacts, and we are now studying to expand our knowledge on metabolic- and proteomic-responses using GS-MS and LC-MS.

• 한국토양비료학회지
• /
• 제40권5호
• /
• pp.377-382
• /
• 2007

완효성을 부여하려는 물질의 외부에 광물입자를 이용하여 피복형 구조체를 제조하고 이의 방출조절능력을 카리비료에 대한 용출실험을 통해 평가하였다. 황산칼륨을 구형의 펠릿으로 제조하고 이의 표면에 평균입도 $13.0{\mu}m$와 $27.4{\mu}m$인 두 가지 장석분말을 이용하여 구조체를 제조하였다. 구조체의 두께와 소성하는 온도를 달리하여 펠릿을 제조하고 용출실험을 통해 구조체의 완효성 발현능력을 평가하였다. 칼륨의 용출은 burst effect효과가 커서 초기 용출율이 높았는데, 소성온도가 낮고 구조체가 얇은 시료에서 크게 나타났다. $1{\sim}55{\mu}m$의 입도를 갖는 장석 보다 $1{\sim}130{\mu}m$의 입도를 갖는 장석에 의한 구조체가 더 높은 충진율에 의해 용출속도를 늦출 수 있었다. 용출속도는 약 7일부터 정상상태의 용출속도를 보이기 시작하였고 용출패턴은 펠릿에 남아있는 칼륨의 농도에 비례하는 first order kinetic의 패턴을 나타내었다. 평균입도 $27.4{\mu}m$인 장석으로 1~2.6mm두께의 피복형 구조체를 만들어 $1050^{\circ}C$에서 소성한 팰릿의 경우에는 40일의 용출율이 약 43%를 나타내었다.