• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.434-437
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• 2003

A new bacterial strain, was isolated from activated sludge, identified and named P. aeruginosa EMS1. The new strain produced surface-active rhamnolipids by batch cultivation in mineral salts medium with waste flying oils. The mutant strain KH7, designated P. aeruginosa EMS1, derived by random mutagenesis with N-methyl-N-nitro-N-nitrosogoanidine treatment producing high levels of the biosurfactants was selected by an ion-pair plate assay. The mutant strain KH7 showed 4-5 times more hydrocarbon emulsification as compared to the parent when grown on waste frying oils and various hydrocarbons. Furthermore, P. aeruginosa EMS1 and mutant strain KH7 was also able to use whey as a co-substrate for growth and biosurfactant production. As results of this study, mutant strain KH7 is a very efficient biosurfactant producer, and its culture conditions are relatively inexpensive and economical. Rhamnolipid is synthesized by the rhlAB-encoded rhamnosyltransferase. To be convinced of these genes, we performed PCR based on P. aeruginosa PAO1 whole-genome database. rhl gene cluster nucleotide and amino acid sequences were compared for both parent and mutant. Comparison of nucleotide sequence of rhlAB, there were usually terminal's codons exchange.

• The Journal of Engineering Geology
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• v.24 no.3
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• pp.431-440
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• 2014

Since the establishment of the Groundwater Act in Korea in 1993, the national policy on groundwater has focused on the preservation and management of groundwater, which should be used only as a subsidiary water resource. However, population growth, increased water demand, climate change, and the need for uniform water distribution have brought changes to groundwater policy, and have led to the prioritization of development projects such as groundwater dams and river bank filtration. Population growth, changes to the water environment, and increased water risks have all played a role in triggering rapid growth within the water industry; the size of the investment in water resources will also continue to increase worldwide. Until now, private wells and bottled mineral water have led the groundwater industry in South Korea. However, a new area of the groundwater industry, which includes the health and medical sciences, employs groundwater properties derived from regional geology, and is growing. This requires the advancement of groundwater research and technical development connected with ICT (Information and Communication Technology) and medical science, and that the public development of groundwater and its various applications is expanded through locating groundwater in the core of the water industry cluster.

• Economic and Environmental Geology
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• v.25 no.1
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• pp.27-39
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• 1992

Several pyrophyllite deposits occur around the Milyang area where Cretaceous andesitic rocks and spatially related granitic rocks are widely distributed. Pyrophyllite ores consist mainly of pyrophyllite, and quartz with small amount of sericite, pyrite, dumortierite, and diaspore. The andesitic rocks and spatially related granitic rocks in this area suggest that they could be formed from the same series of a calc-alkaline magma series. The contents of $SiO_2$, $Al_2O_3$, LOI(loss on ignition) are enriched, and $K_2O$, $Na_2O$, CaO, MgO, $Fe_2O_3$ are depleted in altered andesitic rocks and ores. Enrichment of As, Cr, Sr, V, Sb and depletion of Ba, Cs, Ni, Rb, U, Y, Co, Sc, Zn are characteristic during mineralization. The pyrophyllite ores can be discriminated from the altered-and unaltered wall-rocks by an increasing of $(La/Lu)_{cn}$ from 4.18~22.13 to 8.98~55.05. In R-mode cluster analysis, Yb-Lu-Y, La-Ce-Hf-Th-U-Zr, $TiO_2-V-Al_2O_3$, Sm-Eu, $CaO-Na_2O-MnO$, Cu-Zn-Ag, $K_2O-Rb$ are closely correlated. In the discriminant analysis of multi-element data, $P_2O_5$, As, Cr and $Fe_2O_3$, Sr are helpful to identify the ores from the unaltered-and altered wall-rocks. In the factor analysis, the factors of alteration of andesitic rocks and ore mineralization were extracted. In the change of ions per unit volume, $SiO_2$, $Al^{3+}$ and LOI are enriched and $Na^+$, $K^+$, $Ca^{2+}$, $Mg^{2+}$, $Mn^{2+}$ and $Fe^{3+}$ are depleted during the alteration processes. The Milyang and the Sungjin pyrophyllite deposits could be mineralized by hydrothermal alteration in a geochemical condition of low activity ratio of alkaline ions to hydrogen ion with reference to spatially related granitic rocks.

• The Journal of Engineering Geology
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• v.17 no.3
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• pp.483-494
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• 2007

The properties of fracture system on road-cut slopes along the Busan-Ulsan express way under construction are investigated and analyzed. Fracture spacing distributions show log-normal form with extension fractures and negative exponential form with shear fractures. Straight line segments in log-log plots of cumulative fracture length indicate a power-law scaling with exponents of -1.13 in site 1, -1.01 in site 2 and -1.52 in site 3. It is likely that the stability and strength of rock mass are the lowest in site 1 as judged from the analyses of spacing, density and inter-section of fractures in three sites. In contrast, the highest efficiency of the fracture network for conducting fluid flow is seen in site 3 where the largest cluster occupies 73% through the window map. Based on the field survey data, this study modified weighting values of the RMR system using a multiple regression analysis method. The analysis result suggests a modified weighting values of the RMR parameters as follows; 18 for the intact strength of rock; 61 for RQD; 2 for spacing of discontinuities; 2 for the condition of discontinuities; and 17 for ground water.

• Journal of Practical Agriculture & Fisheries Research
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• v.13 no.1
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• pp.35-46
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• 2011

The effects of organic calcium agent(ECa) tree-spray on the mineral nutrition concentration of petiole and fruit skin and quality in grapevines were studied. The calcium concentration of petiole and fruit skin at harvest in 'Campbell Early' by tree-spray after flowering 14days of calcium agent were increased significantly in calcium chloride 0.4%, ECa 250X and MCa 500X compared to control. The calcium concentration of petiole and fruit skin at harvest by ECa tree-spray after 7 days and 14 days flowering were increased significantly compared to control and 21 days after flowering treatment. Also, The calcium concentration of petiole and fruit skin at harvest by tree-spray of ECa 250X and 125X after flowering were increased significantly compared to control and 500X treatment. Fruit quality, fruit cluster weight, cracking fruit and Bitter rot occurrence by ECa tree-spray of treatment time and concentration different showed no difference between control. Phytotoxicity by tree-spray of ECa concentration different not occur to leaf and fruit.

• Ocean and Polar Research
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• v.26 no.2
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• pp.199-205
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• 2004

$SiO_2$ and CaO are added to decrease the smelting temperature in the reduction-smelting method for manganese nodule processing. These elements are components of the manganese nodules and might be very important controlling factors in the processing due to the locally variable content. The 707 chemical data of manganese nodules acquired from 1994 to 2001 in KODOS(Korea Deep Ocean Survey) area were used for the hierarchical cluster analysis. The chemical data were classified by the morphological types, and the averages of the chemical data for each station were classified by the facies groups and the localities. All data are plotted on the $SiO_2-CaO-MnO$ phase diagram at $1773^{\circ}K$ to compare with the best compositional area in the nodule smelting. Variations and distributions of $SiO_2$ and CaO in KODOS nodules were also reviewed. The mineral phases assigned by the cluster analysis are CFA(Carbonate Fluorapatite), Fe-oxide, Al-silicate, and Mn-oxide. MnO contents are generally higher than $SiO_2$ contents in most of the morphological types except for the Is- and It-type. The Dt- and Tt-type show wider range and the E-types show high anomaly in their CaO contents. The stations which belong to facies group A and B show generally higher MnO contents than $SiO_2$ contents, however, the stations of facies group C and D show wide range in their MnO and $SiO_2$ contents. It seems to be very important to control the $SiO_2$ contents in the processing because of the wide range in the northern area. The additions of approximately 10 wt.% CaO and 10 wt.% $SiO_2$ are recommended for the northern area, whereas, the additions of approximately 10 wt.% CaO and 20 wt.% $SiO_2$ are recommended for the southern area.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.38 no.8
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• pp.887-892
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• 2014

In a conventional magnetic abrasive polishing process, the polishing abrasives are mixed with ferrous particles and slight cutting oil to form a cluster of abrasives. However, when a tool rotates at a high revolution speed, most of the polishing abrasives are scattered away from it due to the increase in centrifugal force. This phenomenon directly reduces the polishing efficiency. The use of a highly viscous matter such as silicone gel instead of cutting oil for mixing is one method to solve this problem and increase abrasive adhesion. Another method to avoid high abrasive scattering is the application of wet magnetic abrasive polishing (WMAP). In WMAP, abundant mineral oil is preliminarily applied to the workpiece surface. This study experimentally evaluated the effect of WMAP on abrasive adhesion. The relationship between the amount of working abrasives and polishing conditions was characterized. Despite the lower adhesion ratio of polishing abrasives, the surface roughness was found to be significantly improved as the result of WMAP.

• Economic and Environmental Geology
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• v.39 no.6 s.181
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• pp.753-770
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• 2006

In urban areas, groundwater pollution is heavily affected by urbanization with land use types. This study aims to characterize groundwater quality and contamination in Sasang industrial area of Busan Metropolitan City where metalworking, machinery and footwear factories are located. Busan Metropolitan City is the highest in the utilization of groundwater resources among the metropolitan cities in Korea. $K^+,\;Na^+,\;Ca^{2+},\;Mg^{2+},\;Cl^-,\;SO_4^{2-}\;and\;HCO_3^-$ concentrations, and electrical conductivity (EC), total dissolved solids (TDS) and salinity are high in the areas near the Nakdong River. The results are attributed to the influence of salt water which intruded into the coastal sediments during sedimentation. In addition, the dominant chemical type of Ca-Cl indicates the influence of salt water in the geological formations as well as anthropogenic pollution. $SiO_2$ ion is interpreted to originate from both water-silicate mineral reactions and the decomposition of cement concretes. Trichloroethylene (TCE) was detected at 12 sites of total 18 sites. However, tetrachloroethylene (PCE) was detected at f sites and 1.1.1-trichloroethane (TCA) at 3 sites. According to the factor analysis, factor 1 was explained by 49.8%, factor 2 19.8%, and factor 3 11.0% with total 80.6% explanation. pH, TDS, salinity, $Ca^{2+},\;K^+,\;Mg^{2+},\;Na^+,\;Al^{3+},\;As^{3+},\;Cl^-\;and\;Fe^{2+}$ were positively highly loaded to factor 1. The chemical components loaded to factor 1 represent the chemical characteristics of both industrial pollution and influence by salt water. Based on the cluster analysis and distribution pattern of chemical components, the concentration of $Na^+,\;Ca^{2+},\;Cl^-,\;SO_4^{2-}\;K^+,\;and\;Mg^{2+}$ is high in the riverside area of the Nakdong River composed of coastal sediments that is influenced by salt water. The downstream area of the Hakjang Stream is judged to be affected by both salt water and artificial pollution. The other part of the study area is interpreted by anthropogenic pollution.

• Journal of Korean Society of Forest Science
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• v.83 no.4
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• pp.486-497
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• 1994

The objective of this study was to examine the effects of air pollution on Pinus thunbergii forests surrounding Sasang industrial complex in Korea. The injury index, contents of chlorophyll and mineral elements, and concentrations of water soluble sulfur in needles were investigated at sample plots surrounding industrial complex and compared with those of control far from industrial complex. The results obtained are as follows ; 1. Discoloration of Pinus thunbergii needles was severe in the vicinity of industrial complex, and the older needle age classes was the more severe its injury appeared. Injury index was increased in the vicinity of industrial complex. 2. Water-soluble sulfur concentration was high in the vicinity of industrial complex at all of needle age classes, and those of all plots were higher than that of control. 3. Chlorophyll a contents were lower at surrounding industrial complex than that at control. It was supposed that chlorophyll a was destroyed by air pollutants. Total chlorophyll contents and content ratio of chlorophyll a to b were influenced by decrease of chlorophyll a contents. 4. Phosphorus contents in needles were decreased even in remoted regions with the increase of needle age classes. Colcium contents in needles were decreased near industrial complex at all needle ages classes. 5. There were negative correlation, between injury index and chlorophyll contents, injury index and calcium contents, and there were positive correlation between injury index and calcium contents. 6. Cluster analysis was carried out to divide the injured regions on sample plots. As a result of the analysis, there were devided 3 regions, severe regions(1-8 plots), medium regions(9, 10, 11, 12, 15, 17 plot), slight(13, 14, 16, 18, 19 plot and control). 7. The cross section of visible injured needle showed the destruction of mesophyll cell, sclerenchyma cell in the outside of resin duct and endodermis, partially.

• Journal of the Korean GEO-environmental Society
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• v.13 no.9
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• pp.31-44
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• 2012

Seasonal changes in groundwater geochemistry exist in Ddan-sum island. In the farming season of April, the spatial distribution of ions explains that $Fe^{2+}$, Fe(total), $Mn^{2+}$, $Ca^{2+}$, $Mg^{2+}$, $SiO_2$, ${HCO_3}^-$, and ${NO_3}^-$ are high in the center of the island, but $Na^+$ and $K^+$ are relatively low and these high anions indicates the effect of fertilizer used for strawberry cultivation. Spatial variation of ion concentration is smaller in August than April because of low agricultural activity and heavy rainfall. Geochemical type of groundwater shows that the center of island has the characteristics of recharge zone but the rim area corresponds to a mixing zone between groundwater and stream water. According to the analysis of saturation index for Fe and Mn ions, hematite, goethite, and rhodochrosite under supersaturation have a possibility of additional mineral deposition, and siderite, $Fe(OH)_3$, manganite, pyrolusite, and pyrochroite under unsaturation may exist as a type of dissolved ion.