• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.6
• /
• pp.894-902
• /
• 2003

Prebiotics (peptides, N-acetyglucoamine, fructo-oligosaccharides, isomalto-oligosaccharides and galactooligosaccharides) were added to skim milk in order to improve the growth rate of contained Lactobacillus acidophilus, Lactobacillus casei, Bifidobacterium longum and Bifidobacterium bifidum. The purpose of this research was to study the potential synergy between probiotics and prebiotics when present in milk, and to apply modern optimization techniques to obtain optimal design and performance for the growth rate of the probiotics using a response surface-modeling technique. To carry out response surface modeling, the regression method was performed on experimental results to build mathematical models. The models were then formulated as an objective function in an optimization problem that was consequently optimized using a genetic algorithm and sequential quadratic programming approach to obtain the maximum growth rate of the probiotics. The results showed that the quadratic models appeared to have the most accurate response surface fit. Both SQP and GA were able to identify the optimal combination of prebiotics to stimulate the growth of probiotics in milk. Comparing both methods, SQP appeared to be more efficient than GA at such a task.

• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.7
• /
• pp.967-972
• /
• 2004

Four Holstein$\times$Indigenous cows with ruminal canulas were used in a 4$\times$4 Latin square design with 28 d periods to determine the effect of replacing nonforage fiber source (NFFS) from cassava residues for non-fiber carbohydrates (NFC) on ruminal fermentation characteristics and milk production. Dietary treatments contained 17% forage neutral detergent fiber (FNDF) from corn silage and 0, 3, 6 and 9% nonforage NDF from cassava residues and 11% nonforage NDF from other NFFS, so that levels of nonforage NDF were 11, 14, 17 and 20% dry matter (DM). Intakes of DM and net energy for lactation, average daily gain and milk fat percentage were not different (p>0.05). Ruminal pH, ammonia concentrations, acetate to propionate ratios, 24 h in sacco fiber digestibility significantly increased with increasing contents of nonforage NDF from cassava residues. Concentrations of VFA, urinary excretion of purine derivatives, milk protein percentage, production of milk and 4% FCM significantly decreased. These results suggest that NFC in diets is one of the limiting factors affecting productivity of dairy cows in the tropics and thus NFFS is better used as partial replacements for FNDF.

• Korean Journal of Pharmacognosy
• /
• v.51 no.4
• /
• pp.297-301
• /
• 2020

Silymarin (SM) and silybin diastereomers (SD) in milk thistle (Silybum marianum) were determined using high-performance liquid chromatography and quantified using a reverse-phase column in a gradient elution system. UV detection was performed at 288 nm. The content of SM and SD in milk thistle was 3.236 and 0.553 mg/g DW, respectively. Determining the presence and quantifying the content of SM and SD in milk thistle are vital for the pharmaceutical industry to identify optimal sources for developing health supplements or therapeutics.

• Korean Journal of Animal Reproduction
• /
• v.12 no.2
• /
• pp.91-96
• /
• 1988

A study was conducted to improve breeding efficiency of dairy and beef cows on Cheju Island. Milk progesterone and plasma progesterone profiles were determined by using radioimmunoassay techniques during the oestrus cycle, onset on oestrus and during the gestation period. Progesterone connections in milk were very low during the oestrus with a means value of 2.4ng/ml and 4.6ng/ml at the onset of oestrus but gradually declined at 4 to 9 hr after oestrus. In the cyclic cow, milk progesterone concentraitons in the early part of the cycle, ranged from 3 to 5ng/ml and increased from 6.0 to 11.6ng/ml on day 13 to 21 of cycle. Milk progesterone concentrations of pregnancy cows ranged from 5.0 to 40ng/ml during the gestation and grom basal line to 3.5ng/ml for nonpregnant cows. Plasma progesterone concentrations of beef during pregnancy period ranged form 3.0 to 33ng/ml and basal levels ranged from 0 to less than 2.5ng/ml for non-pregnant beef cows.

• Asian-Australasian Journal of Animal Sciences
• /
• v.11 no.2
• /
• pp.117-120
• /
• 1998

Relationships of breeding values of sires for first lactation milk yield with pedigree information or indices were examined to identify the optimal criteria of selecting young dairy bulls for future use in artificial insemination (AI). Records of performance data on 1087 crossbred daughters (Holstein - Friesian, Jersey and Brown Swiss with Hariana) of 147 sires, generated at Livestock Production Research (Cattle and Buffaloes) Farm, IVRI, Izatnagar, U.P., during 1972 - 1995 were used to obtain the estimates of sire's breeding values (EBV) using the Best Linear Unbiased Prediction Procedures. The correlations between young bull's EBV and the dam's first lactation milk yield was non-significantly different from zero. However, the young bull's EBV was negatively and significantly related (r = - 0.275 ; P < 0.05) to the dam's best lactation milk yield, suggesting that the selection of young dairy bulls from high yielding elite dams is not a suitable criteria for genetic improvement. The correlations of sire's and paternal grandsire's EBV's with young bull's EBV were high and positive (0.532, 0.844; P < 0.01). The maternal grandsire's EBV was positively but non-significantly related to grandson's EBV. The pedigree index incorporating dam's milk records and sire's EBV's showed a negative and non-significant correlation with young bull's EBV. However, the correlation of a pedigree index $(I_3)$ combining information on sire's and paternal grand-sire's EBV's with young bull's EBV's was considerably high and positive (0.797; P < 0.01). The regression coefficients of young bull's EBV on pedigree index $I_3$, was higher than those on other pedigree information. These results revealed that there was no advantage in basing selection on dam's performance or maternal grand-sire's EBV and that sire's and paternal grandsire's EBV's were reliable pedigree information for selection of young dairy bulls for future use in AI.

• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.10
• /
• pp.1400-1410
• /
• 2004

Eight crossbred (75% Holstein Friesian) cows in mid-lactation were randomly assigned to a switchback design with a 2x2 factorial arrangement to evaluate two nonstructural carbohydrate (NSC) sources (corn meal and cassava chips) with different rumen degradability and used at two levels of NSC (55 vs. 75%) with protein source (supplied by urea in the concentrate mix). The treatments were 1) Low degradable low level of corn (55%) 2) Low degradable high level of corn (75%) 3) High degradable low level of cassava (55%) and 4) High degradable high level of cassava (75%). The cows were offered the treatment concentrate at a ratio to milk yield at 1:2. Urea-treated rice straw was offered ad libitum as the roughage and supplement with 1 kg/hd/d cassava hay. The results revealed that total DM intake, BW and digestion coefficients of DM were not affected by either level or source of energy. Rumen fermentation parameters; NH3-N, blood urea nitrogen and milk urea nitrogen were unaffected by source of energy, but were dramatically increased by level of NSC. Rumen microorganism populations were not affected (p>0.05) by source of energy, but fungal zoospores were greater for cassava-based concentrate than corn-based concentrate. Milk production and milk composition were not affected significantly by diets containing either source or level of NSC, however concentrate than corn-based concentrate averaging (4.4 and 4.2, respectively). Likewise, income over feed, as estimated from 3.5% FCM, was higher on cassava-based concentrate than corn-based concentrate averaging (54.0 and 51.4 US$/mo, respectively). These results indicate that feeding diets containing either cassava-based diets and/or a higher of oncentrates up to 75% of DM with NPN (supplied by urea up to 4.5% of DM) can be used in dairy rations without altering rumen ecology or animal performance compared with corn-based concentrate.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.8
• /
• pp.1151-1158
• /
• 2008

This study was designed to examine the effects of different dietary protein levels on growth performance, nutrient utilization, amino acid (AA) digestibility and serum biochemical parameters of preruminant dairy calves. Fifteen healthy new-born calves were randomly allotted to three experimental groups and fed with different milk replacer that contained 18% (LP), 22% (MP) or 26% (HP) of protein. The results showed that final BW, net gain and ADG were significantly higher in the MP group than in LP and HP groups (p<0.05). In addition, the apparent digestibility of CP in the MP group was significantly higher than in the other two groups (p<0.05). The values of N intake and fecal N excretion were significantly increased following the increase of dietary protein content (p<0.05). However, in all three groups of animals, dietary protein content had no significant effect on urinary N concentration (p>0.05). BUN concentration, on the other hand, decreased as calves grew but increased following the increase of dietary CP content. Furthermore, no significant differences in digestibility of amino acids were observed among these three groups of animals (p>0.05). We concluded that calves fed with milk replacer containing 22% of protein had better growth performance and nutrient utilization as compared to animals treated with milk replacer containing either 18% or 26% of protein.

• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.11
• /
• pp.1573-1578
• /
• 2002

This study was conducted to investigate the effects of diets that contained different levels of fungal treated wheat straw on the intake, digestibility and performance of lactating cows. Eight primiparous Holstein cows, in late lactation ranging from $170{\pm}10$ days in milk and yielding $14.3{\pm}1.3$ kg/d of fat corrected milk (FCM) were allocated into four diets with 0, 10, 20 and 30% fungal (Pleurotus ostreatus coded P-41) treated wheat straw in a $4{\times}4$ Latin Square experiment. The daily intake of DM, OM, DOM, CP and TDN were not affected by substitution of alfalfa hay with fungal treated wheat straw. Inclusion of the treated straw at different levels in the diet did not affect the digestibility of nutrients, except for the ADF that was significantly (p<0.05) reduced in the diet contained 30% treated straw. The types of the diet did not significantly affect daily milk and FCM production. The milk composition including fat, protein, lactose, solid non-fat (SNF) and total solid (TS) were not statistically (p>0.05) different among the diets. All cows gained weight, but the inclusion of treated straw to the diet significantly (p<0.05) increased the body weight gain and the highest amount was obtained in the diet containing 20% treated. Inclusion of fungal treated wheat straw up to 30% of the diet of lactating cows supplemented with a protein source such as cottonseed meal had not affected the nutrients intake and lactation performance.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.4
• /
• pp.457-463
• /
• 2000

Fifty mid-lactation Holstein cows were used in a six-week feeding trial to study effects of high-forage, high-fat diets on dry matter intake and production performance. Cows were divided into 10 replicates, each consisting of five cows. Each cow was assigned to a control (diet 1) or one of the four experimental diets (high-forage (75%), high-fat (7.5%) (diet 2); high-forage, medium-fat (5%) (diet 3); medium forage (65%), high-fat (diet 4); medium-forage, medium-fat (diet 5)), or a control diet containing about 50% forage and 2% fat. All diets were isonitrogenous (17.7% crude protein). The forage mixture consisted of 20% alfalfa hay, 40% alfalfa haylage, and 40% com silage. Supplemental fat included 80% rumen-protected fat and 20% yellow grease. Dry matter intake was decreased (p<0.01) in cows fed experimental diets (18.4, 20.9, 19.9, and 22.6 kg for cows fed diets 1-4, respectively vs. 27.5 kg for cows fed the control diet). Daily milk production was lower (p<0.05) for cows consuming experimental diets (30.5, 31.3, 31.0, and 32.5 kg for cows fed greater for cows consuming experimental diets (1.74, 1.55, 1.60, and 1.53 kg milk/kg dry matter intake for cows fed diets 1-4, respectively, vs. 1.26 kg milk/kg dry matter intake for cows fed the control diet).

• Asian-Australasian Journal of Animal Sciences
• /
• v.7 no.3
• /
• pp.383-387
• /
• 1994

Six heifers each of $F_1$, $F_2$, $F_3$, $F_4$ H. Friesian $\times$ Sahjwal halfbreds and pure Sahiwal with overall average initial age and live weight of $315.6{\pm}134.8 days$ and $143.8{\pm}48.5kg$, respectively were used for this study. The under trial animals were fed according to N.R.C. (1978). The overall average age at maturity, first conception and first calving was $563.8{\pm}116.8$, $675.8{\pm}135.6$ and $956.8{\pm}149.8days$ with live weights as $302.2{\pm}58.6$, $342.6{\pm}41.5$ and $433.9{\pm}38.1kg$, respectively. The overall 305 days and total milk yield for 1st lactation was $2,729.0{\pm}669.3$ and $2,992.7{\pm}377.5litre$ while the FCM (at 4%) was $2,934.2{\pm}410.8litre$. The lactation length was $336.6{\pm}69.6days$. The fat and solids not fat contents were $4.5{\pm}0.2$ and $8.2{\pm}0.3percent$, respectively. The milk production in Sahiwal cows was significantly lower than crossbred cows. The services per conception were $2.5{\pm}1.3$. The overall per head per day consumption of DM, TCP and ME was $10.9{\pm}1.2kg$, $1,399{\pm}199gm$ and $22.6{\pm}2.4 M.Cal.$, respectively. The overall milk producing efficiency for the production of one litre of FCM was $1.12{\pm}0.15kg$ of DM, $142.2{\pm}17.76gm$ of TCP and $2.31{\pm}0.27M.Cal.$ of energy while the feeding cost was Rs. $1.46{\pm}0.22$ per litre of FCM produced. The feeding cost per litre FCM was significantly higher in Sahiwal and $F_2$ than in other genetic groups.