• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2001년도 발생공학 국제심포지움 및 학술대회 발표자료집
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• pp.59-59
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• 2001

• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1252-1256
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• 2006

This study estimated the effects of parity (1-3) and stage of lactation (early, mid and late) on daily milk yield (DMY), somatic cell score (SCS), milk urea nitrogen (MUN), blood glucose, and immunoglobulin G (IgG), their heritabilities and genetic correlations between them in Holsteins (n = 200). Means and standard deviations of DMY, SCS, MUN, blood glucose, and IgG in the experimental herd were $23.35{\pm}7.75kg$, $3.81{\pm}2.00$, $13.99{\pm}5.68mg/dl$, $44.91{\pm}13.12mg/dl$, and $30.36{\pm}6.72mg/ml$, respectively. DMY was the lowest in first parity, and in late lactation. SCS increased with parity; however, it was lowest in mid-lactation. MUN was lowest in first parity, and no difference was noted across stage of lactation. Blood glucose was similar between parities, however the highest blood glucose was observed during mid lactation. IgG level was significantly different between first and second parity; however, stage of lactation did not affect its level. Heritability of DMY was 0.16. Its genetic correlations with SCS and with blood glucose were -0.67 and 0.98, respectively. Heritability of SCS was 0.15. Genetic correlations of SCS with MUN, glucose, and IgG were -0.72, -0.59, and 0.68, respectively. Heritability of MUN was estimated to be 0.39 and had a genetic correlation of -0.35 with IgG. Heritabilities of blood glucose and IgG were 0.21 and 0.33, respectively. This study suggested that MUN, blood glucose and IgG could be considered important traits in future dairy selection programs to improve milk yield and its quality with better animal health and welfare. However, further studies are necessary involving more records to clarify the relationship between metabolic and immunological traits with DMY and its quality.

• Journal of Nutrition and Health
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• 제30권1호
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• pp.3-11
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• 1997

This study was conducted to investigate the effects of protein levels and casein/whey ratios on organ growth and protein metabolism in early weaned rats. Premature rats weaned by the 17th day were fed six semipurified synthetic, isocaloric and gel diets that contained three levels (low, medium and high) and two different combinations(casein/whey ; 80 : 20 or 20 : 80) of milk protein for 8 days. On the 25th day postpartum, frest weigth and DNA, RNA and milk protein contents in brain, liver, kidney and muscle were determined to ascertain organ and cellular growth. Futher, with a view to ascertain protein metabolism and renal functions, serum total protein, $\alpha$-amino N, urea N, and creatinine and creatinine and urinary urea N, creatinine and hydroxproline were determined. Total DNA contents of brain, liver and kidney, which may represent as an index of cell numbers in those organs were significantly decreased in the rats fed diets containing low level protein regardless of casein/whey ratio. However, as fat as the rats fed high protein diets were concerned, their fresh weight, protein contents and GFR of kidney were significantly increased. Furthermore, nitrogen components, $\alpha$-amino N, urea N and creatinie in serum and urine were also increassed. Another observation was that high casein/whey ratio significantly facilitated accumulation of porteins in muscle and kidney and urinary hydorxyproline excretion, not affecting the DNA content of those organs. This study showed that low(8%) or high(32%) contents of protein had less desirable effects either on protein metabolism or on organ cellular growth in prematurely weaned rats, whereas there were no effects on general growth and bone strength.

• Asian-Australasian Journal of Animal Sciences
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• 제21권9호
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• pp.1303-1311
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• 2008

Twenty four early lactating Nili-Ravi buffaloes, eight in each group, were used in a Randomized Complete Block Design to evaluate the influence of varying ruminally degradable protein (RDP) to ruminally undegradable protein (RUP) ratio on feed intake, digestibility, N balance, milk yield and its composition, conception rate and days open. Three experimental diets were formulated to contain RDP:RUP of 50:50, 66:34 and 82:18 and were denoted as HRUP, MRUP and LRUP, respectively. Dry matter (DM) intake was higher (p<0.05) in buffaloes fed HRUP diet than in those fed MRUP and LRUP diets. Dry matter digestibility was higher (p<0.05) in buffaloes fed LRUP diet than in those fed HRUP and MRUP diets. Linear increase was observed in DM digestibility with increasing RDP:RUP while Neutral detergent fiber digestibility remained unaltered in buffaloes fed HRUP and MRUP diets, however, it was higher than in those fed LRUP diet. Crude protein digestibility remained unaltered across all treatments. Milk and 4 percent fat corrected milk (4% FCM) yield was higher (p<0.05) in buffaloes fed HRUP diet than those fed MRUP and LRUP diets. Linear decrease in milk yield was observed with increased RDP:RUP. Milk protein and fat yields were higher (p<0.05) in animals fed HRUP diet than those fed MRUP and LRUP diets. Milk protein percent in animals fed HRUP diet was higher than in those fed LRUP diet, whereas it did not differ with those fed MRUP diet. Percent of fat, total solids, solid not fat and lactose remained unaltered across all diets. Nitrogen balance was higher in buffaloes fed HRUP diet than in those fed other diets. Increasing the RDP:RUP resulted in a linear decrease in N balance. The blood urea nitrogen and milk urea nitrogen were lower (p<0.05) in buffaloes fed HRUP diet than those fed MRUP and LRUP diets. The blood pH remained unaltered across all treatments. Days open did not differ significantly. Conception rate was higher in buffaloes fed HRUP diet than those fed MRUP and LRUP diets. The findings of the present study indicate that feeding high (50% of the total crude protein) ruminally undegradable protein diet not only increased nutrient intake and milk yield but also improved conception rate in early lactating buffaloes.

• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1529-1534
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• 2004

The nutritive value of lentil straw (Lens culinaris) relative to 4% urea treated wheat straw (w/w) was assessed in adult bucks and milking buffaloes on-station and on-farm, respectively. A digestion-cum-nitrogen balance experiment was conducted with three bucks (24.35 kg) fed ad libitum lentil straw (LS), urea treated wheat straw (UTS) and a 1:1 (w/w DM basis) mixture of LS and UTS (LS:UTS) in a 3$\times$3 Latin square design. Though daily dry matter intake (g kg $W^{0.75}$ or % LW) of bucks fed LS and UTS did not differ significantly, that of LS:UTS was significantly (p<0.05) low. Similarly, digestibility of nutrients (DM, OM and CP) and nitrogen balance (g/day) did not differ significantly for bucks given LS or UTS although a significant positive improvement was evident in these parameters for LS:UTS. The digestibility of fibre fractions (NDF and ADF) was significantly (p<0.05) lower in LS as compared to UTS or LS:UTS. Except for the significantly (p<0.05) lower intake of DCP (g kg$W^{0.75}$), the bucks fed LS had statistically similar nutrient intake as compared to UTS. Feeding of LS:UTS resulted in significantly higher nutrient (DCP, TDN) intake by bucks. The important blood-biochemical parameters (haemoglobin,packed cell volume, glucose, urea nitrogen, total protein) and activity of serum enzymes (alanine aminotransferase and aspartate aminotransferase) did not differ significantly in goats irrespective of dietary treatment. In the onfarm study, eighteen multiparous milking buffaloes were randomly assigned into three equal groups to assess the relative effect of feeding untreated wheat straw (WS), UTS and LS:UTS ad libitum as basal feed with a supplement. The study continued for 3 months and revealed that daily dry matter intake and average milk production (liters/day) by buffaloes offered LS:UTS and UTS was statistically similar but it was significantly higher compare to WS. However, milk composition did not differ significantly among the dietary treatments. It may be concluded that the nutritive value of lentil straw appeared to be no different to UTS, however, a positive synergistic effect was evident by feeding a mixture of LS and UTS on performance of goats and lactating buffaloes.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 발생공학 국제심포지움 및 학술대회 발표자료집
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• pp.59-59
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• 2001

This study was designed to assess effect of MUN concentration on reproduction performance and monitoring of feeding and fertility management in commercial dairy herd. The mean of milk yield is 26.48±8.38㎏ per day, milk fat 3.80±0.58%, protein 3.13±0.3% MUN 16.68±5.87㎎/㎖ and somatic cell 392,000±77,060㎖. Milk yield has been shown that negative correlation with fat, protein and somatic cell(P〈0.01). The finding of this study was significant relationship between non-pregnant days and MUN concentration. (omitted)

• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1549-1554
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• 2001

The application of rumen undegradable intake protein (UIP) on lactating dairy goats was studied. Thirty 2-year-old lactating dairy goats were selected and assigned to dietary treatments begun from the third week to the fourth month postpartum. Experimental diets were formulated into three, low (32% CP), med (35% CP) and high (38% CP), iso-nitrogenous (16% CP) and iso-energetic ($NE_L$ 1.68 Mcal/kg) UIP levels. Results showed that feed intake was not significantly different among the treatment groups. The milk yield in the High UIP group (3.17 kg) was significantly higher than the med (2.95 kg) and low UIP (2.45 kg) groups (p<0.05). The milk compositions, milk fat, milk lactose and milk solids-non-fat (SNF) showed no significant differences among the three treatment groups. The milk protein however was significantly (p<0.05) lower in the low UIP than in the other treatment groups. The milk urea-N was significantly (p<0.05) higher in the low UIP than in the other treatment groups. The mean serum aspartate amino transferase (AST), urinary-N and total protein concentrations were significantly (p<0.05) lower in the high and the med UIP groups than in the low UIP group.

• Asian-Australasian Journal of Animal Sciences
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• 제15권3호
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• pp.330-339
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• 2002

Fifty one Nili-Ravi dairy buffaloes in their last two months of gestation were selected. After parturition, rectal examination of reproductive organs was carried out until the occurrence of the first oestrus (PEI). Milk samples were analyzed for milk progesterone levels (MPL). Ovulation (POI) was confirmed by rectal palpation and MPL. Feed and blood samples were collected fortnightly and analyzed. Body condition score (BCS) was recorded on a scale of 0 to 5. Crude protein (CP) intake varied among different seasons and correlated positively with serum urea levels, POI (p<0.01) and PEI (p<0.05). Excess CPI was lower in the group showing oestrus as compared to those remaining as anoestrus (p<0.05). The dietary ratio of crude protein - metabolizable energy (CP:ME) in the oestrus animals was narrow and constant, while the anoestrus animals had a widely fluctuating one. In normal breeding season (NBS) calvers, mean serum urea level (SUL) was lower than the low breeding season (LBS) calvers. SUL was positively correlated with PEI and POI (p<0.01). Up to six months postpartum, SUL were constantly higher in anoestrus than oestrus buffaloes. Mean metabolizable energy (ME) intake was lower in the NBS calvers than the LBS calvers (p<0.01). BCS and postpartum ovulation interval were correlated with ME intake (p<0.01). Prepartum ME intake was higher in oestrous as compared to anoestrous animals (p<0.05). Higher and lower ME intakes were associated with anoestrus, while a moderate energy intake was associated with a PEI of less than 75 days. Buffaloes with poor BCS belonged to the LBS calving group and most of the NBS calving buffaloes had good BCS. BCS was negatively correlated with PEI (p<0.01) and was higher in oestrous buffaloes than anestrus. It was concluded that excess intake of crude protein, associated with higher serum urea levels and low energy intake, associated with poor body condition, are the key factors for low reproductive efficiency. It may be corrected by adopting a proper feeding strategy.

• 농업과학연구
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• 제48권1호
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• pp.45-57
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• 2021

This study aimed to determine the effects if ambient temperature (Ta), relative humidity (RH), and temperature-humidity index (THI) on milk production parameters such as milk yield, fat, protein, milk urea nitrogen (MUN), and lactose. A total of fifty (50) mid-lactating Korean Holstein cows (144 - 150 days postpartum) were randomly assigned to two groups in equal numbers (n = 25) according to the type of diet received: conventional feed, a mixture of 56: 44 ratio of roughage and concentrate mix, and organic feed containing 100% organic roughage. Temperature, RH, and THI were recorded daily and averaged monthly. Conventional group showed higher dry matter intake throughout the experiment and thus, showed higher total milk yield and milk composition such as milk fat, protein, and lactose content than organic milk. Milk of the conventional group showed higher polyunsaturated fatty acid concentration. Highest milk yield was observed in September (21.7℃, 82.4%RH) in conventional group (27.69 kg·day^-1) and in June (21.4℃, 72.9%RH) in organic group (14.31 kg·day^-1). In conventional group, milk yield was positively correlated with Ta and THI, and milk protein was negatively correlated with Ta, RH, and THI. In organic feeding, only the MUN among milk composition parameters showed a significant correlation where it showed a negative correlation with Ta, RH, and THI. Conventional feeding showed significantly higher milk yield, fat, protein, and lactose than organic feeding throughout the experiment, although, organic feeding showed to be less likely affected by Ta, RH, and THI than the conventional feeding.

• 대한지역사회영양학회지
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• 제25권6호
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• pp.485-501
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• 2020

Objectives: The purpose of this study was to examine the biochemical characteristics and dietary intake of adolescents aged 12 to 18 years according to the frequency of milk consumption. Methods: Data from the 2010~2011 Korea National Health and Nutrition Examination Survey was used for the study. The study examined adolescents' (12~18 years) demographic characteristics (house income level, residence region, skipping or not-skipping of breakfast/lunch/dinner, eating-out frequency), anthropometric characteristics (height, weight, weight status), biochemical characteristics (fasting plasma glucose, blood urea nitrogen, creatine, triglycerides, cholesterol, HDL-cholesterol, hemoglobin, hematocrit) and nutrient intakes through quantitative and qualitative evaluation using the Korean Dietary Reference Intakes (KDRI), index of nutrition quality (INQ), nutrition adequacy ratio (NAR) of 3 groups (< 1/week, 1~6/week, ≥ 1/day) according to the frequency of milk consumption. Results: There were significant differences in gender and income levels among the 3 groups. There were no differences in height, weight, and weight status among groups. There were differences in biochemical characteristics and nutrient intake. In boys, there were differences in the mean of BUN and HDL-cholesterol, in quantitative intakes of riboflavin, calcium, phosphorus, potassium by KDRI levels, in qualitative intakes of riboflavin, calcium, phosphorus by INQ and riboflavin, calcium, phosphorus by NAR among 3 groups. In girls, there were differences in the mean of blood urea nitrogen, creatine, HDL-cholesterol, in quantitative intakes of protein, riboflavin, calcium, phosphorus by KDRI levels, in qualitative intakes of riboflavin, calcium, phosphorus by INQ and riboflavin, calcium, phosphorus by NAR among the 3 groups. Conclusions: In Korean adolescents, boys had a higher frequency of milk consumption than girls, and higher the income level, higher the frequency of milk consumption. Consumption of milk appeared to have a positive association with triglycerides, HDL-cholesterol, and indices related to muscle mass. Regular consumption of milk is an important factor in enhancing the intake of riboflavin, calcium, and phosphorus, which adolescents lack. The results of the study indicate a need to prepare an environment and education program to increase milk consumption in adolescents at home and school.