• Title/Summary/Keyword: Migration test

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Single Cell Gel Electrophoresis (comet assay) to Detect DNA Damage and Apoptosis in Cell Level (DNA damage와 Apoptosis를 정량화하는 단세포전기영동법)

  • 류재천;김현주;서영록;김경란
    • Environmental Mutagens and Carcinogens
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    • v.17 no.2
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    • pp.71-77
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    • 1997
  • The single cell gel electrophoressis(SCGE) assay, also known as the comet assay, is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakage in mammalian cells. The SCGE or comet assay is a promising test for the detection of DNA damage and repair in individnal cells. It has widespread potential applications in DNA damage and repair studies, genotoxicity testing and biomonitoring. In this microgel electrophoresis technique, cells are embedded in agarose gel on microscope slides, iysed and electrophoresed under alkaline conditions. Cells with increased DNA damage display increased migration of DNA from the nucleus towards the anode. The length of DNA migration indicates the amount of DNA breakage in the cell. The comet assay is also capable of identifying apoptotic cells which contain highly fragmented DNA. Here we review the development of the SCGE assay, existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA and the potential applications of the technique.

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PPGA for the Optimal Load Planning of Containers (컨테이너의 최적 적하계획을 위한 PPGA)

  • Kim, Kil-Tae;Cho, Seok-Jae;Jin, Gang-Gyoo;Kim, Si-Hwa
    • Journal of Navigation and Port Research
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    • v.28 no.6
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    • pp.517-523
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    • 2004
  • The container load planning is one of key factors for efficient operations of handling equipments at container ports. When the number of containers are large, finding a good solution using the conventional genetic algorithm is very time consuming. To obtain a good solution with considerably small effort, in this paper a pseudo-parallel genetic algorithm(PPGA) based on both the migration model and the ring topology is developed The performance of the PPGA is demonstrated through a test problem of determining the optimal loading sequence of the containers.

Material Characteristics of High-Strength Concrete Incorporating High Replacement Level of Blast-Furnace Slag (고로슬래그를 다량 함유한 고강도 콘크리트의 재료적 특성)

  • Lee, Hoi-Keun;Jung, Jae-Hong;Kim, Han-Joon;Lee, Seung-Hoon
    • Proceedings of the Korea Concrete Institute Conference
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    • 2010.05a
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    • pp.291-292
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    • 2010
  • In this study, high-strength concrete mixtures were made with blast-furnace slag of 50% and 70% replacement level to evaluate material properties including compressive strength development, adiabatic temperature rise, autogenous shrinkage and chloride-ion migration coefficient. Test results showed that the use of high percentage blast-furnace slag in high-strength concrete can reduce heat of hydration and chloride-ion migration coefficient, result in control thermal cracking and improve durability performance especially under high corrosive environment.

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A study on heavy metal migration in fly ash/bentonite using a reactive transport model

  • Jung, Yoo-Jin;Cho, Hee-Chan
    • 한국지구물리탐사학회:학술대회논문집
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    • 2003.11a
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    • pp.629-636
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    • 2003
  • The retardation of heavy metals in a mixture of fly ash and bentonite was studied as a potential barrier material for a landfill. Column tests were conducted using synthetic leachate having 100 mg/L and 50 mg/L of lead (Pb) and cadmium (Cd), respectively. Results indicated that the mixture had obvious retardation ability for heavy metals. To investigate the retardation factor caused by adsorption, batch adsorption tests were conducted at various concentrations. Test results were correlated with both Langmuir and Freundlich isotherms. The adsorption of the lead ion was applicable to the Langmuir isotherm and the adsorption of the cadmium ion was applicable to the Freundlich isotherm. In addition, based on experimental results, the migration characteristics of heavy metals through the bed of fly ash and bentonite mixture were investigated using the PHREEQC, a reactive transport model, under the real conditions of the landfill liner.

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A New Approach to Adaptive HFC-based GAs: Comparative Study on Crossover Genetic Operator (적응 HFC 기반 유전자알고리즘의 새로운 접근: 교배 유전자 연산자의 비교연구)

  • Kim, Gil-Sung;Choi, Jeoung-Nae;Oh, Sung-Kwun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.9
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    • pp.1636-1641
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    • 2008
  • In this study, we introduce a new approach to Parallel Genetic Algorithms (PGA) which combines AHFCGA with crossover operator. As to crossover operators, we use three types of the crossover operators such as modified simple crossover(MSX), arithmetic crossover(AX), and Unimodal Normal Distribution Crossover(UNDX) for real coding. The AHFC model is given as an extended and adaptive version of HFC for parameter optimization. The migration topology of AHFC is composed of sub-populations(demes), the admission threshold levels, and admission buffer for the deme of each threshold level through succesive evolution process. In particular, UNDX is mean-centric crossover operator using multiple parents, and generates offsprings obeying a normal distribution around the center of parents. By using test functions having multimodality and/or epistasis, which are commonly used in the study of function parameter optimization, Experimental results show that AHFCGA can produce more preferable output performance result when compared to HFCGA and RCGA.

Determination of Plasticizers in the Gasket of Glass Bottle for Bulgogi Sauces (불고기 소스용 유리병 가스켓에서의 가소제 정량)

  • Lee, Jung-Pyo;Lee, Keun-Taik
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.15 no.2
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    • pp.61-65
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    • 2009
  • This study was conducted to investigate the safety status of gasket of glass bottle being used for the Bulgogi sauce. The content of plasticizers in the gasket and their migrated amounts into Bulgogi sauce were determined. Among the plasticizers analyzed, only di-isodecyl phthalate (DIDP) was detected in the range between 30.6 and 35.9% from 6 samples. None of the plasticizers was detected in the sauce. Therefore, it can be concluded that the safety status of the gasket tested met the requirement of limit values as prescribed for the migration test of food packaging utensils, containers and packages of the Korea Food Code.

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Analysis of Five Years of US Immigration Medical Exams (5년간의 미국 이민 비자 신체검사 결과 분석)

  • Lim, Juwon
    • Korean journal of aerospace and environmental medicine
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    • v.31 no.3
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    • pp.77-81
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    • 2021
  • Purpose: The United States of America have pre-migration screening program are mandated through legislation. The one of purpose of this program is to prevent the importation of certain communicable diseases. Examinations of migration applicants are performed by panel physicians who are licensed physicians in the countries of origin that have agreements with the government departments of the country of destination to undertake this activity. In recent decades, the number of US migrants has increased, however COVID-19 impacted this trend of international migrants. The Aim of this study is to examine trends of US applicants and outcomes of tuberculosis and sexual transmitted infections (STIs) screening in South Korean applicants for US visa. Methods: A total of 4,442 applicants participated in US visa health check-up in 2016 to 2020. Results: The numbers of applicant for US visa in males and females was 1,814 and 2,628. The positive results in tuberculosis and STIs screening was noted with 0.66% in nontuberculous mycobacteria (NTM), 0.03% in tuberculosis, 0.03% in gonorrhea, 0.08% in syphilis, and 0.74% in chlamydia. The age groups of 2 to 14 years showed higher positive rate (13.89%) in tuberculin skin test (TST) tests compared to the rate of interferon gamma release assay (IGRA) tests (0.44%, P<0.001). The positive ratio of NTM and abnormal chest X-ray (CXR) which required sputum tests among the applicants over 15 years old has trends to increase with age. The age groups of over 70 years showed high positive rate in NTM and abnormal CXR (4.10%, 20.51%). However, ages 15 to 49 years groups showed 0.00% to 0.30% in NTM and 1.08% to 3.91% in abnormal CXR. In the study population, the positive rates of STIs were 0.03%, 0.08%, and 0.74% in gonorrhea, syphilis, and chlamydia, respectively. Among the participants who underwent sputum smears and cultures were just 1 case of active tuberculosis (0.03%) and 25 cases of NTM infection (0.66%). Conclusion: This study found that rate of positivity in tuberculosis and STIs screening among South Korean applicants for US visa in 2016 to 2020. Regarding tuberculosis, the positivity of CXR among the aged 15 to 59 years applicants was lower than that among over 60 years old. Among 2 to 14 years, positivity of IGRA was very low. And IGRA test can reduce effort of further evaluation compared to TST test in South Korea, so IGRA test among young adults can be useful. High positive rates in STI among young adult could result in high active sexuality, especially chlamydia infection is most common. Therefore Chlamydia polymerase chain reaction should be considered formal protocol among the Korean applicant for US visa.

The Effect of Entrained Air Contents on the Properties of Freeze-thaw Deterioration and Chloride Migration in Marine Concrete (연행 공기량이 해양콘크리트의 동결융해 및 염화물 확산특성에 미치는 영향)

  • Park, Sang-Joon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.12 no.5
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    • pp.161-168
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    • 2008
  • The freeze-thaw deterioration and chloride attack, which are the typical degradation factors for durability of marine concrete, are significantly affected by pore structures in terms of penetration and diffusion. These pore structures of concrete are closely related to the types and amount of AE agent, used to guarantee the resistance of freeze-thaw deterioration, and the elapsed time before concrete pouring. This paper evaluates the durability of concrete based on the results of tests on cylinder specimens and core specimens from mock-up members with different air content of 4~6% and 8~10%, respectively. According to the test results, the air content of hardened concrete is 2.5~5.2% at 7 days and 2.4~5.1% at 28 days. These air contents are about half of the initial values just after the concrete mixing. Judging from the amount of scale after the freeze-thaw test completed, air content of 8~10% is slightly more beneficial against the deterioration of concrete than air content of 4~6%. Meanwhile, the core specimens from mock-up members exhibit somewhat unfavorable freeze-thaw deterioration and chloride migration characteristic compared with the cylinder specimens tested in the laboratory under the same mixing condition, as to show 106% in freeze-thaw test and 160% in chloride diffusion coefficient test, respectively.

Verification of Practicality for the SSBL and Pinger Synchronizing Biotelemetry Method and System through the Tracking of Fish (어류의 행동추적에 의한 SSBL · 핑거동기 바이오텔레메터리 방식과 시스템의 실용성 검증)

  • Park, Ju-Sam
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.40 no.1
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    • pp.78-85
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    • 2004
  • The new biotelemetry method and system that the installation and the treatment of equipment is convenient and the instantaneously detailed position of the fish attached a pinger is able to track comparatively easily had been developed, an practicality of it were verified in the water tank and the small sea port through the tracking of fish. The biotelemetry method had been gotten the three dimensional locations of fish to the receiving transducer by combining of the super short base line (SSBL) method to detect the direction of pinger and the pinger synchronizing method to measure the range from receiving transducer to pinger. The receiving system had been designed to realize the high precision or wide detection range by application of the basic design method for receiving system of biotelemetry and the hydrophone array configuration. From tracking test of carp in the water tank, the migration course and the velocity of carp was investigated and the observed migration course was compared with measurement. The measured migration course of carp coincided with the observation in the main and the position of carp was able to track three dimensionally. The velocity of carp measured by the moving average method was 11.2cm/s. From tracking test of yellowtail in the small sea port, the migration course and the velocity of yellowtail was investigated at natural condition. The position of yellowtail was able to track three dimensionally and the velocity of it measured by moving average method was 43.9cm/s.

Accelerated Life Test and Data Analysis of the Silver Through Hole Printed Wiring Board (가속수명시험을 이용한 은도통홀 인쇄회로기판의 신뢰성연구)

  • 전영호;권이장
    • Journal of Korean Society for Quality Management
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    • v.25 no.2
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    • pp.15-27
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    • 1997
  • This paper describes a highly accelerated life test (HALT, USPCBT) method for rapid qualification testing of STH PWB(Silver Through Hole Printed Wiring Boards). This method was carried out to be an alternative to the present time-consuming standard 1344 hours life testing(THB). The accelerated life test conditions were $121^{\circ}C$/95%R.H. at 50V bias and without bias. Their results are compared with those observed in the standard 1344 hours life test at $40^{\circ}C$/95%R.H. at 50V bias and without bias. The studies were focused on the samples time-to-failure as well as the associated conduction and failure mechanisms. The abrupt drop of insulation resistance is due to the absorption of water vapour. And the continuous drop of insulation resistance is due to the Ag migration. The ratios of time-to-failure of HALT(USPCBT) to THB were 25 and 11 at 50V bias and without bias respectively.

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