• 제목/요약/키워드: Migration Preparation

검색결과 31건 처리시간 0.029초

쥐 근조직의 Cytochrome Oxidase에 대한 비교 연구 (Comparative Study on Cytochrome Oxidase of Rat Muscle Tissues)

  • Song, Eun-Sook
    • 한국동물학회지
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    • 제29권1호
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    • pp.70-74
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    • 1986
  • 쥐 골격근의 크루드 미토콘드리아에 있는 시토크롬 옥시다제의 활성을 비교 하였다. 붉은색의 빠른 트윗치 근육은 가장 높은 효소 활성을 나타냈고, 흰색의 빠른 트읫치 근육은 가장 낮았으며, 붉은 색이며 느린 트윗치 근육은 그 중간이었다. 위 세가지 타입의 근육에서 힘 염색한 결과 시토크롬 옥시다제의 전기영동상의 이동성이 다르게 나타났다. 이동성의 순서는 타입 I > 타입 $II_A$ > 타입 $II_B$이었다. 면역학적 전기영동의 결과는 위의 결과를 뒷받침 하였다.

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Low Temperature Suspension Polymerization of Methyl Methacrylate for the Preparation of High Molecular Weight Poly(methyl methacrylate)/Silver Nanocomposite Microspheres

  • Yeum, Jeong-Hyun;Ghim, Han-Do;Deng, Yulin
    • Fibers and Polymers
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    • 제6권4호
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    • pp.277-283
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    • 2005
  • In order to prepare high molecular weight poly(methyl methacrylate) (PMMA)/silver nanocomposite microspheres, methyl methacrylate was suspension-polymerized in the presence of silver nanoparticles at low temperature with 2,2'-azobis(2,4-dimethylvaleronitrile) as an initiator. The rate of conversion was increased by increasing the initiator concentration. When silver nanoparticles were added, the rate of polymerization decreased slightly. High monomer conversion (about $85\%$) was obtained in spite of low polymerization temperature of $30^{\circ}C$. Under controlled conditions, PMMA/silver microspheres with various number-average degrees of polymerization (6,000-37,000) were prepared. Morphology studies revealed that except for normal suspension microspheres with a smooth surface, a golf ball-like appearance of the microspheres was observed, due to the migration and aggregation of the hydrophilic silver nanoparticles at the sublayer beneath the microsphere's surface.

생활중심 교수학습 모형의 설계와 적용 - '인구이동과 인구변화' 단원을 중심으로 - (Design and Application of the Teaching-Learning Model on Highschool Student's Daily Life : A Case Study of Migration and Population Change Unit in Highschool)

  • 옥한석;장현숙
    • 한국지역지리학회지
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    • 제11권4호
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    • pp.523-535
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    • 2005
  • 이 연구는 학습자들의 일상적인 생활 속에서 얻는 지리적 경험에 기초한 생활중심 교수학습의 모형 설계와 적용 가능성을 살펴보려고 한다. 생활중심 교수학습 모형의 효과를 실증적으로 검증하기 위하여 실험집단과 비교집단을 비교하여 수행하였다. 교수학습의 실제사례로 고등학교 2학년의 '인구이동과 인구변화'의 단원을 선정하였다. 실험 수업대상으로는 강원도 춘천시 C고등학교 2학년을 대상으로 하며 생활중심 교수학습 모형을 적용한 실험집단 2개 반과 적용하지 않은 비교집단 2개 반 모두 4개반의 122명이 선정되었다. 연구 결과는 다음과 같다. 첫째, 교사가 교수할 개념들을 체계화된 지식 구조로 교수하지 않을 경우 이에 대한 학습자의 인지구조 재구성은 보다 활발하게 이루어 질 수 없기 때문에, 고등학교 한국지리의 '인구이동과 인구변화' 단원의 지리개념은 상위개념인 이동에서, 기본개념인 이주, 하위개념인 이주 요인으로 위계화하고 학생들이 경험할 수 있는 일상적 지리경험을 알아보았다. 둘째, 지리과 생활 중심 교수학습 내용의 실제 사례로 학생 자신의 가족 이주사 및 가족수의 변화를 조사하는 과제학습지를 기초적 자료로 활용하였다. 셋째, 이 연구에서 개발한 수업모형은 암기식 수업보다 학업성취도 측면에서 효과가 더 높게 나타났다. 수업반응도 검사를 분석한 결과, 정의적인 측면에서는 수업의 흥미도, 만족도 수업내용의 활용성에서는 긍정적인 효과가 나타났다. 특히 이러한 수업모형에 의한 학습이 학생들의 흥미를 유발시킬 수 있다는 것을 알 수 있다. 그러나 지리적 탐구력 신장에는 도움이 되지 않았다.

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Study on Extraction of Mucopolysaccharide-protein Containing Chondroitin Sulfate from Chicken Keel Cartilage

  • Shin, S.C.;You, S.J.;An, B.K.;Kang, C.W.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권4호
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    • pp.601-604
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    • 2006
  • The objective of this study was to investigate technical methods for extraction of mucopolysachharide-protein containing chondroitin sulfate from keel cartilage of chickens. The chemical composition of chicken keel cartilage was determined. For the preparation of mucopolysaccharide-protein from lyophilized chicken keel cartilage, hot water extraction and alcalase hydrolysis methods were examined. Results showed that the optimum condition of hot water extraction was incubation for 120 min with a yield of 40.09% and chondroitin sulfate content of 28.46%. For alcalase hydrolysis, the most effective condition was 2% alcalase in 10 volumes of distilled water for 120 min. The yield of hydrolysate was 75.87%, and chondroitin sulfate content was 26.61%. For further separation of chondroitin sulfate from the alcalase hydrolysate, which has a higher yield than that of hot water, 60% ethanol precipitation was performed. The yield of the ethanol precipitate was 21.41% and its chondroitin sulfate content was 46.31%. The hot water extract, alcalase hydrolysate and ethanol precipitate showed similar electrophoretic migration with standard chondroitin sulfate (chondroitin sulfate A), using cellulose acetate membrane electrophoresis. These results indicated that a significant amount of mucopolysaccharide-protein containing chondroitin sulfate could be acquired form chicken keel cartilage. Therefore, keel cartilage in chicken may provide an inexpensive source of chondroitin sulfate for commercial purposes.

고분자 경화제를 사용한 라이너와 HTPB 추진제의 접착력 및 접착공정 개선 (Improvement of Bonding Process and Bond Strength of HTPB Propellant/Liner using a Polymeric Curative)

  • 정병훈;서태석;홍명표
    • 한국추진공학회:학술대회논문집
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    • 한국추진공학회 2005년도 제25회 추계학술대회논문집
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    • pp.413-416
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    • 2005
  • 고분자경화제를 사용한 라이너와 HTPB 추진제의 접착력 및 접착공정을 개선연구를 수행하였다. HTPB와 TDI를 반응시켜 제조된 고분자경화제를 라이너에 사용하면, 접착계면에서 경화제의 이동현상이 줄어든다. 따라서 연소관 준비공정에서 내열재 연마 및 물질이동방지제 도포공정의 생략이 가능하며 HTPB 추진제와 라이너의 접착력이 증가되었다. 또한 가속노화 시험결과 접착력의 저하현상이 관찰되지 않았다.

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고분자 경화제를 사용한 라이너와 HTPB 추진제의 접착력 및 접착공정 개선 (Improvement of Bonding Process and Bond Strength of HTPB Propellant/Liner using a Polymeric Curative)

  • 정병훈;서태석;홍명표
    • 한국추진공학회지
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    • 제10권2호
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    • pp.110-114
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    • 2006
  • 고분자경화제를 사용한 라이너와 HTPB 추진제의 접착력 및 접착공정의 개선연구를 수행하였다. HTPB와 TDI를 반응시켜 제조된 고분자경화제를 라이너에 사용하면, 접착계면에서 경화제의 이동현상이 줄어든다 따라서 연소관 준비공정에서 내열재 연마 및 물질이동방지제 도포공정의 생략이 가능하며 HTPB 추진제와 라이너의 접착력이 증가되었다. 또한 가속노화 시험결과 접착력의 저하현상이 관찰되지 않았다.

Test Execution Variation in Peritoneal Lavage Cytology Could Be Related to Poor Diagnostic Accuracy and Stage Migration in Patients with Gastric Cancer

  • Ki, Young-Jun;Ji, Sun-Hee;Min, Jae Seok;Jin, Sung-Ho;Park, Sunhoo;Yu, Hang-Jong;Bang, Ho-Yoon;Lee, Jong-Inn
    • Journal of Gastric Cancer
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    • 제13권4호
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    • pp.214-225
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    • 2013
  • Purpose: Peritoneal lavage cytology is part of the routine staging workup for patients with advanced gastric cancer. However, no quality assurance study has been conducted to show variations or biases in peritoneal lavage cytology results. The aim of this study was to demonstrate a test execution variation in peritoneal lavage cytology between investigating surgeons. Materials and Methods: A prospective cohort study was designed for determination of the positive rate of peritoneal lavage cytology using a liquid-based preparation method in patients with potentially curable advanced gastric cancer (cT2~4/N0~2/M0). One hundred thirty patients were enrolled and underwent laparotomy, peritoneal lavage cytology, and standard gastrectomy, which were performed by 3 investigating surgeons. Data were analyzed using the chi-square test and a logistic regression model. Results: The overall positive peritoneal cytology rate was 10.0%. Subgroup positive rates were 5.3% in pT1 cancer, 2.0% in pT2/3 cancer, 11.1% in pT4a cancer, and 71.4% in pT4b cancer. In univariate analysis, positive peritoneal cytology showed significant correlation with pT stage, lymphatic invasion, vascular invasion, ascites, and the investigating surgeon. We found the positive rate to be 2.1% for surgeon A, 10.2% for surgeon B, and 20.6% for surgeon C (P=0.024). Multivariate analysis identified pT stage, ascites, and the investigating surgeon to be significant risk factors for positive peritoneal cytology. Conclusions: The peritoneal lavage cytology results were significantly affected by the investigating surgeon, providing strong evidence of test execution variation that could be related to poor diagnostic accuracy and stage migration in patients with advanced gastric cancer.

Inhibitory Effects of Licochalcone A and Isoliquiritigenin on Monocyte Adhesion to TNF-$\alpha$-activated Endothelium

  • Kwon Hyang-Mi;Lim Soon Sung;Choi Yean-Jung;Jeong Yu-Jin;Kang Sang-Wook;Bae Ji-Young;Kang Young-Hee
    • Nutritional Sciences
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    • 제8권3호
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    • pp.153-158
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    • 2005
  • Numerous natural herbal compounds have been reported to inhibit adhesion and migration of leukocytes to the site of inflammation Licorice extracts, which have been widely used in traditional Chinese medicinal preparation, possess various pharmacological effects. Isoliquiritigenin, a biogenetic precursor of flavonoids with various pharmacological effects, is a natural pigment present in licorice. We attempted to explore whether licorice extracts and isoliquiritigenin mitigate monocyte adhesion to tumor necrosis factor-$\alpha$ (TNF-$\alpha$)-activated human umbilical vein endothelial cells (HUVEC). In addition, it was tested whether the inhibition of monocyte adhesion to the activated HUVEC accompanied a reduction in vascular cell adhesion molecule-l expression(VCAM-l). Dry-roasted licorice extracts in methylene chloride but not in ethanol markedly interfered with THP-l monocyte adhesion to INF-$\alpha$-activated endothelial cells. licochalcone A compound isolated from licorice extract in methylene chloride appeared to modestly inhibit the interaction of THP-l monocytes and activated endothelium. In addition, isoliquiritigenin abolished the monocyte adhesion with attenuating VCAM-l protein expression on HUVEC induced by INF-$\alpha$. These results demonstrated that non-polar components from dry-roasted licorice extracts containing licochalcone A as well as isoliquiritigenin were active in blocking monocyte adhesion to cytokine-activated endothelimn, which appeared to be mediated most likely through the inhibition of VCAM-l expression on HUVEC. Therefore, licorice may hamper initial inflammatory events on the vascular endothelium involving induction of endothelial cell adhesion molecules.

리튬이온 이차전지용 금속이온 선택성 술폰화 폴리아릴렌에테르술폰 공중합체-폴리올레핀 함침격리막 제조 및 특성 (Preparation and Characterization of Sulfonated Poly (Arylene Ether Sulfone) Random Copolymer-Polyolefin Pore-filling Separators with Metal Ion Trap Capability for Li-ion Secondary Battery)

  • 정연태;안주희;이창현
    • 멤브레인
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    • 제26권4호
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    • pp.310-317
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    • 2016
  • 리튬이온 이차전지는 리튬이온이 이동하면서 전기화학적 충방전사이클을 완성하는 에너지변환장치를 의미한다. 리튬이온 이차전지는 높은 에너지밀도와 낮은 자가방전률, 상대적으로 긴 수명주기 등 다양한 장점을 갖는다. 최근 전기차 수요증가는 고용량 리튬이온 이차전지 개발을 촉진하고 있으나 음극에서의 dendrite 형성으로 인한 전기적 단락 현상과 전지 폭발 문제와 같은 심각한 안전문제를 야기한다. 또한, 리튬이온 이차전지 구동시 상승된 온도에서 폴리올레핀계열(예 : 폴리에틸렌과 폴리프로필렌) 격리막의 열수축 문제가 발생한다. 이와 같이 낮은 열 안정성은 리튬이온 이차전지의 성능과 수명의 감소로 이어진다. 본 연구에서는 폴리올레핀계열 함침격리막 제조를 위한 중요한 소재로서 술폰화 폴리아릴렌에테르술폰 랜덤 공중합체를 사용하였으며, 제조된 격리막을 이용하여 dendrite 형성과 관련된 금속이온 흡착 능력과 리튬이온전도성, 열적 내구성이 평가되었다.

Changes of Isoflavones and Fatty Acids in Eoyukjang, a Traditional Korean Fermented Soysauce Prepared under the Ground in a Pot or an Incubator

  • Park, Yong-Woo;Lee, Seung-Wook;Chang, Pahn-Shick;Lee, Jong-Mee;Kim, Young-Suk;Lee, Jae-Hwan
    • Food Science and Biotechnology
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    • 제18권1호
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    • pp.48-52
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    • 2009
  • Eoyukjang is a traditional Korean sauce made of cooked soypaste or meju supplemented with fish and meats at least 1 year of fermentation period. Eoyukjang was recovered according to the traditional method and stored under the ground in a pot without plastic packaging (13G: 13 month fermentation under the ground) or in an incubator wrapped with plastic packaging (6I, 12I, and 18I: 6, 12, and 18 month fermentation, respectively). Distribution of isoflavones and fatty acids were monitored by high performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Total isoflavones in 13G, 6I, 12I, and 18I were 3.792, 0.387, 0.460, and $0.510{\mu}mol/g$, respectively. Samples of 13G had at least 8.24 times higher isoflavone contents than samples from 12I. Aglycones were the major isoflavones in eoyukjang and were found more than 92% in 13G and 39-63% in incubated samples. In fatty acid analysis, the ratios of unsaturated to saturated fatty acids from 13G were higher than those from 6I, 12I, and 18I. Traditional fermentation methods using a pot may allow more migration of air and moisture than samples wrapped with plastic packaging, which caused the difference in the distribution of isoflavones and fatty acids.