• Title/Summary/Keyword: Microscopic test

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Prevalence of Leptospiral Antibodies in Sows (모돈에서의 Leptospira속균에 대한 혈중항체조사)

  • 강신석;곽학구;김홍기
    • Korean Journal of Veterinary Service
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    • v.16 no.1
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    • pp.65-69
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    • 1993
  • A serological survey was conducted to detect the type of Leptospirosis in sows. Antigen(live antigen) tested were L.icterohemorrhagiae, L.pomonal, L.canicola, L.Hardjo, L.australis, L.autumnalis, L.grippotyphosa, L.pyrogens, L.bataviae, L.hebdomadis. The survey was performed from J one 1992 to December 1992 by microscopic-agglutination test. The results were as follows 1, Among the serum samples of 92 heads of the sows, 6 heads of the sow(6.5%) were positive. 2. Among the positive samples of 6 heads, L.icterihemorrhagiae were 4 heads(4.3%) and L. pomona 2 heads (2.2%), respectively. 3. Antibody titers of positive sera were ranging from 1:200 to 1:1600. 4. The seroprevalence of leptospira in Chechon city, Chechon county, Danyang county that Chechon county(3.3%) was higher than that of other districts(1% -2.2%).

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Failure Analysis on SCM435 High Strength Bolt of Forging Die (단조 금형 SCM435 고장력 볼트의 파손 해석)

  • Yun, Seo-Hyun;Kim, Min-Heon;Nam, Ki-Woo
    • Journal of the Korean Society of Industry Convergence
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    • v.22 no.6
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    • pp.649-655
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    • 2019
  • Fracture behaviors of SCM435 high strength bolt have been studied including macroscopic and microscopic fracture observation, Energy Dispersive X-ray Spectroscopy, Vickers hardness test and applied stress evaluation. cracks (ratchet marks) were generated by the repetitive loads acting on the bolts, initial stress of bolt and the stress concentration. The applied stress was found to be slightly higher than the fatigue limit of the material. The initial stress of bolt must be removed, and the mold temperature during the process must be maintained by room temperature. Bolts are recommended to be peened to improve fatigue limit.

Failure Investigation of Fire-Side Water-Wall Tube Boiler

  • Fatah, M.C.;Agustiadi, D.;Pramono, A.W.
    • Corrosion Science and Technology
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    • v.20 no.5
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    • pp.242-248
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    • 2021
  • Unforeseen failures of boilers in power plants may affect the continuation of electricity generation. Main failures in boilers are influenced by the tube material, tube position, boiler service temperature and pressure, and chemical composition of the feed water and coal. This investigation was intended to find answers on the causes and mechanism of failure of the fire-side boiler water-wall tubes, due to perforation and corrosion. The tube conformed to the material requirements in terms of its chemical composition and hardness. Microscopic examination showed ferrite and pearlite indicating no changes in its microstructure due to the temperature variation. SEM test showed a single layer and homogenous film density particularly on the area far from perforation. However, layers of corrosion product were formed on the nearby perforation area. EDX showed that there were Na, Ca, S, and O elements on the failed surface. XRD indicated the presence of Fe2O3 oxide. The failure mechanism was identified as a result of significant localized wall thinning of the boiler water wall-tube due to oxidation.

Classification of Mouse Lung Metastatic Tumor with Deep Learning

  • Lee, Ha Neul;Seo, Hong-Deok;Kim, Eui-Myoung;Han, Beom Seok;Kang, Jin Seok
    • Biomolecules & Therapeutics
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    • v.30 no.2
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    • pp.179-183
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    • 2022
  • Traditionally, pathologists microscopically examine tissue sections to detect pathological lesions; the many slides that must be evaluated impose severe work burdens. Also, diagnostic accuracy varies by pathologist training and experience; better diagnostic tools are required. Given the rapid development of computer vision, automated deep learning is now used to classify microscopic images, including medical images. Here, we used a Inception-v3 deep learning model to detect mouse lung metastatic tumors via whole slide imaging (WSI); we cropped the images to 151 by 151 pixels. The images were divided into training (53.8%) and test (46.2%) sets (21,017 and 18,016 images, respectively). When images from lung tissue containing tumor tissues were evaluated, the model accuracy was 98.76%. When images from normal lung tissue were evaluated, the model accuracy ("no tumor") was 99.87%. Thus, the deep learning model distinguished metastatic lesions from normal lung tissue. Our approach will allow the rapid and accurate analysis of various tissues.

Reciprocating Wear Test of AISI 52100 Bearing Steel in h-BN-based Aqueous Lubricants

  • Gowtham Balasubramaniam;Dae-Hyun Cho
    • Tribology and Lubricants
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    • v.39 no.6
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    • pp.228-234
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    • 2023
  • In this study, reciprocating wear tests are performed on AISI 52100 bearing steel to investigate its tribological behavior in a hexagonal boron nitride (h-BN) water solution. The h-BN-based aqueous lubricant is prepared using an atoxic procedure called ultrasonic sonication in pure water. Ball-on-flat reciprocating sliding experiments are conducted, where the ball is slewed on a fixed flat at 50-㎛ displacement. The lubricating behavior of h-BN is compared with that of deionized (DI) water. Results show that the friction coefficient is higher in h-BN testing than that in DI tests, but the results are equalized as the friction coefficient reaches a stable level. Scanning electron microscopic images reveal significant material loss in the center and mild abrasion on the edge of the wear scar in h-BN tests. However, these effects are minor in DI water situations. The results of energy-dispersive X-ray spectroscopy show that considerable oxidation occurs in the central zone of the wear scar in h-BN cases with strong adhesion and material removal. These findings reveal the importance of determining ideal circumstances that can tolerate material friction and wear.

The effect of conditioning by Tetracycline-Hcl on implant surface;The SEM study and. surface roughness measurements : RBM surface (염산 테트라싸이클린이 RBM적용 임프란트 표면구조에 미치는 영향의 미세구조 및 표면 거칠기 변화에 관한 연구)

  • Lim, Hae-Soo;Park, Joon-Bong;Kwon, Young-Hyuk;Herr, Yeek;Chung, Jong-Hyuk
    • Journal of Periodontal and Implant Science
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    • v.37 no.3
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    • pp.585-597
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    • 2007
  • The present study was performed to evaluate the effect of Tetracycline-HCI and Saline on the change of implant surface microstructure and surface roughness according to application time. Implants with resorbable blasting media surface were utilized. Before test all 13 implants were measured surface roughness. Among them, 6 implants were rubbed with 50mg/ml Tetracycline-HCl solution and other 6 implants with saline for $\frac{1}{2}$min., 1min., $1\frac{1}{2}$min., 2min., $2\frac{1}{2}$min and 3min. Then, specimens were processed for scanning electron microscopic observation and surface roughness after test. The results of this study were as follows. 1. Control group showed a few irregular, rough, uneven surface with crater-like depression. 2. The test group with Tetracycline-HCl conditioning showed an altered surface when Tetracycline-HCl was applied for 30secs, and showed a various surface alteration as application times go on. 3. The test group with Saline conditioning showed no significant surface differences and surface roughness. 4. The significant increase of Ra value was showed when Tetracycline-HCl was applied for 30secs. In conclusion, the 50mg/ml Tetracycline-HCl must not be applied for the RBM surface implant for surface treatment.

Comparative Assessment of Diagnostic Performances of Two Commercial Rapid Diagnostic Test Kits for Detection of Plasmodium spp. in Ugandan Patients with Malaria

  • Bahk, Young Yil;Park, Seo Hye;Lee, Woojoo;Jin, Kyoung;Ahn, Seong Kyu;Na, Byoung-Kuk;Kim, Tong-Soo
    • Parasites, Hosts and Diseases
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    • v.56 no.5
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    • pp.447-452
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    • 2018
  • Prompt diagnosis of malaria cases with rapid diagnostic tests (RDTs) has been widely adopted as an effective malaria diagnostic tool in many malaria endemic countries, primarily due to their easy operation, fast result output, and straightforward interpretation. However, there has been controversy about the diagnostic accuracy of RDTs. This study was conducted to evaluate the diagnostic performances of the 2 commercially available malaria RDT kits, RapiGEN Malaria Ag Pf/Pv (pLDH/pLDH) and Asan $EasyTest^{TM}$ Malaria Ag Pf/Pv (HRP-2/pLDH) for their abilities to detect Plasmodium species in blood samples collected from Ugandan patients with malaria. To evaluate the diagnostic performances of these 2 RDT kits, 229 blood samples were tested for malaria infection by microscopic examination and a species-specific nested polymerase chain reaction. The detection sensitivities for P. falciparum of Malaria Ag Pf/Pv (pLDH/pLDH) and Asan $EasyTest^{TM}$ Malaria Ag Pf/Pv (HRP-2/pLDH) were 87.83% and 89.57%, respectively. The specificities of the 2 RDTs were 100% for P. falciparum and mixed P. falciparum/P. vivax infections. These results suggest that the 2 RDT kits showed reasonable levels of diagnostic performances for detection of the malaria parasites from Ugandan patients. However, neither kit could effectively detect P. falciparum infections with low parasitaemia (<$500parasites/{\mu}l$).

Application of Nondestructive Technique on Hydrogen Charging Times of Stainless Steel 304L (스테인리스 304L강의 수소장입시간에 대한 비파괴기법 적용)

  • Lee, Jin-Kyung;Hwang, Seung-Kuk;Lee, Sang-Pill;Bae, Dong-Su;Son, Young-Seok
    • Journal of Power System Engineering
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    • v.19 no.5
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    • pp.60-66
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    • 2015
  • Embrittlement of material by hydrogen charging should be cleared for safety of storage vessel of hydrogen and components deal with hydrogen. A stainless steel is generally used as materials for hydrogen transportation and storage, and it has a big advantage of corrosion resistance due to nickel component in material. In this study, microscopic damage behavior of stainless steel according to the hydrogen charging time using nondestructive evaluation was studied. The surface of stainless steel became more brittle as the hydrogen charging time increased. The parameters of nondestructive evaluation were also changed with the embrittlement of stainless steel surface by hydrogen charging. Ultrasonic test, which is the most generalized nondestructive technique, was applied to evaluate the relationship between the ultrasonic wave and mechanical properties of stainless steel by hydrogen charging. The attenuation coefficient of ultrasonic wave was increased with hydrogen charging time because of surface embrittlement of stainless steel. In addition, acoustic emission test was also used to study the dynamic behavior of stainless steel experienced hydrogen charging. AE event at the hydrogen charged specimen was obviously decreased at the plastic zone of stress-strain curves, while the number of event for the specimen of hydrogen free was dramatically generated when compared with the specimens underwent hydrogen charging.

High Temperature Creep Characteristics Evaluation for Degraded Heat Resistance Steel of Power Plant by Mini-Specimen (미소시험편에 의한 재질열화된 내열강의 고온 크리프 특성 평가)

  • Lyu, Dae-Young;Baek, Seung-Se;Yu, Hyo-Sun
    • Korean Journal of Materials Research
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    • v.13 no.7
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    • pp.429-435
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    • 2003
  • In this study the new creep test using miniaturized specimen(10${\times}$10${\times}$0.5 ㎣) was performed to evaluate the creep characteristics for degraded materials of 2.25Cr-1Mo steel. For this creep test, the artificially aged materials for 330 hrs and 1820hrs at $630^{\circ}C$ were used. The test temperatures applied for the creep deformation of miniaturized specimens was X$630^{\circ}C$ and the applied loads were between 45 kg∼80 kg. After creep test, macro- and microscopic observation were conducted by the scanning electron microscope(SEM). The creep curves depended definitely on applied load and microstructure and showed the three stages of creep behavior like uniaxial tensile creep curves. The load exponents of virgin, 330 hrs and 1820 hrs materials based on creep rate showed 14.8, 9.5 and 8.3 at $550^{\circ}C$ respectively, The 1820 hrs material showed the lowest load exponent and this behavior was also observed in the case of load exponent based on creep rupture time. In contrast to virgin material which exhibited fined dimple fractography, a lot of carbides like net structure and voids were observed on the fractography of degraded materials.

Characterization of the Soldering Interface in Power Modules by Peel Strength Measurement (벗김강도 측정법에 의한 파워 모듈의 솔더접합 특성 평가)

  • Kim, Nam-Kyun;Lee, Hee-Heung;Bahng, Wook;Seo, Kil-Soo;Kim, Eun-Dong
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.16 no.12
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    • pp.1142-1149
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    • 2003
  • The strength and characteristics of the soldering interface of the power semiconductor chip in a power module has been firstly surveyed by the peel strength measurement method. A power module is combined with several power chips which generally has 30∼400$\textrm{mm}^2$ chip area to allow several tens or bigger amps in current rating, so that the traditional methods for interface characterization like shear test could not be applied to high power module. In this study power diode modules were fabricated by using lead-tin solder with 10${\times}$10$\textrm{mm}^2$ or 7${\times}$7$\textrm{mm}^2$ soldering interface. The peel strengths of soldered interfaces were measured and then the microscopic investigation on the fractured surfaces were followed. The peel test indicated that the crack propagated either through the bulk of the soft lead-tin solder which has 55-60 kgf/cm peel strength or along the interface between the solder and the plated nickel layer which has much lower 22 kgf/cm strength. This study showed that the peel test would be a useful method to quantify the solderability as well as to recognize which is the worst interface or the softest material in a power module with a large soldering area.