• Title/Summary/Keyword: Microscopic image analysis

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Rietveld Analysis of Nano-crystalline MnFe2O4 with Electron Powder Diffraction

  • Kim, Jin-Gyu;Seo, Jung-Wook;Cheon, Jin-Woo;Kim, Youn-Joong
    • Bulletin of the Korean Chemical Society
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    • v.30 no.1
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    • pp.183-187
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    • 2009
  • The structure of nano-crystalline $MnFe_2O_4$ was determined and refined with electron powder diffraction data employing the Rietveld refinement technique. A nano-crystalline sample (with average crystal size of about 10.9 nm) was characterized by selected area electron diffraction in an energy-filtering transmission electron microscope operated at 120 kV. All reflection intensities were extracted from a digitized image plate using the program ELD and then used in the course of structure refinements employing the program FULLPROF for the Rietveld analysis. The final structure was refined in space group Fd-3m (# 227) with lattice parameters a=8.3413(7) $\AA$. The reliability factors of the refinement are $R_F$=7.98% and $R_B$=3.55%. Comparison of crystallographic data between electron powder diffraction data and reference data resulted in better agreement with ICSD-56121 rather than with ICSD-28517 which assumes an initial structure model.

Confocal Laser Endomicroscopy in the Diagnosis of Biliary and Pancreatic Disorders: A Systematic Analysis

  • Do Han Kim;Somashekar G. Krishna;Emmanuel Coronel;Paul T. Kroner;Herbert C. Wolfsen;Michael B. Wallace;Juan E. Corral
    • Clinical Endoscopy
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    • v.55 no.2
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    • pp.197-207
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    • 2022
  • Background/Aims: Endoscopic visualization of the microscopic anatomy can facilitate the real-time diagnosis of pancreatobiliary disorders and provide guidance for treatment. This study aimed to review the technique, image classification, and diagnostic performance of confocal laser endomicroscopy (CLE). Methods: We conducted a systematic review of CLE in pancreatic and biliary ducts of humans, and have provided a narrative of the technique, image classification, diagnostic performance, ongoing research, and limitations. Results: Probe-based CLE differentiates malignant from benign biliary strictures (sensitivity, ≥89%; specificity, ≥61%). Needle-based CLE differentiates mucinous from non-mucinous pancreatic cysts (sensitivity, 59%; specificity, ≥94%) and identifies dysplasia. Pancreatitis may develop in 2-7% of pancreatic cyst cases. Needle-based CLE has potential applications in adenocarcinoma, neuroendocrine tumors, and pancreatitis (chronic or autoimmune). Costs, catheter lifespan, endoscopist training, and interobserver variability are challenges for routine utilization. Conclusions: CLE reveals microscopic pancreatobiliary system anatomy with adequate specificity and sensitivity. Reducing costs and simplifying image interpretation will promote utilization by advanced endoscopists.

Microscopic Traffic Analysis of Freeway Based on Vehicle Trajectory Data Using Drone Images (드론 영상을 활용한 차량궤적자료 기반 고속도로 미시적 교통분석)

  • Ko, Eunjeong;Kim, Soohee;Kim, Hyungjoo
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.20 no.6
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    • pp.66-83
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    • 2021
  • Vehicles experience changes in driving behavior due to the various facilities on the freeway. These sections may cause repetitive traffic congestion when the traffic volume increases, so safety issues may be raised. Therefore, the purpose of this study is to perform microscopic traffic analysis on these sections using drone images and to identify the causes of traffic problems. In the case of drone image, since trajectory data of individual vehicles can be obtained, empirical analysis of driving behavior is possible. The analysis section of this study was selected as the weaving section of Pangyo IC and the sag section of Seohae Bridge. First, the trajectory data was extracted through the drone image. And the microscopic traffic analysis performed on the speed, density, acceleration, and lane change through cell-unit analysis using Generalized definition method. This analysis results can be used as a basic study to identify the cause of the problem section in the freeway. Through this, we aim to improve the efficiency and convenience of traffic analysis.

Emulation of Anti-alias Filtering in Vision Based Motion Mmeasurement (비전 센서의 앨리어싱 방지 필터링 모방 기법)

  • Kim, Jung-Hyun
    • The Journal of Korea Robotics Society
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    • v.6 no.1
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    • pp.18-26
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    • 2011
  • This paper presents a method, Exposure Controlled Temporal Filtering (ECF), applied to visual motion tracking, that can cancel the temporal aliasing of periodic vibrations of cameras and fluctuations in illumination through the control of exposure time. We first present a theoretical analysis of the exposure induced image time integration process and how it samples sensor impingent light that is periodically fluctuating. Based on this analysis we develop a simple method to cancel high frequency vibrations that are temporally aliased onto sampled image sequences and thus to subsequent motion tracking measurements. Simulations and experiments using the 'Center of Gravity' and Normalized Cross-Correlation motion tracking methods were performed on a microscopic motion tracking system to validate the analytical predictions.

The excimer laser ablation of PET for micro-mold insert - The control of cross sectional shape using Fourier optics - (마이크로 금형 제작을 위한 PET의 엑시머 레이저 어블레이션 - 퓨리에 광학을 이용한 가공 단면 형상의 제어 -)

  • Shin, Dong-Sik;Lee, Je-Hoon;Seo, Jung;Kim, Do-Hoon
    • Laser Solutions
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    • v.6 no.3
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    • pp.19-28
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    • 2003
  • The manufacturing process for the microfluidic device can include sequential steps such as master fabrication, electroforming, and injection molding. The laser ablation, using masks, has been applied to the fabrication of channels in microfluidic devices. In this research, an excimer laser was used to engrave microscopic channels on the surface of PET (polyethylene terephthalate), which shows a high absorption ratio for an excimer laser beam with a wavelength of 248 m. When 50-${\mu}{\textrm}{m}$-wide rectangular microscopic channels are ablated with a 500 ${\times}$ 500 ${\mu}{\textrm}{m}$ square mask at a magnification ratio of 1/10, ditch-shaped defects were found in both corners. The measurement of laser beam intensity showed that a coherent image in the PET target caused such defects. Analysis based on the Fourier diffraction theory enabled the prediction of the coherent shape at the image surface as well as the diffraction beam shape between the mask and the image surface. It also showed that the diameter of the aperture had a dominant effect. The application of aperture with a diameter of less than 3 mm helped to eliminate such defects in the ablated rectangular microscopic channels on PET without such ditch-shaped defects.

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A Glance of Electron Tomography through 4th International Congress on Electron Tomography (International Congress on Electron Tomography에 대한 간략한 이해와 현황)

  • Rhyu, Im-Joo;Park, Seung-Nam
    • Applied Microscopy
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    • v.38 no.3
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    • pp.275-278
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    • 2008
  • Electron tomography (ET) is an electron microscopic technique for obtaining a 3-D image from any electron microscopy specimen and its application in biomedical science has been increased thanks to development of electron microscopy and related technologies during the last decade. There are few researches on ET in Korea during this period. Although the importance of ET has been recognized recently by many researchers, initial approach to electron tomographic research is not easy for beginners. The 4th International Congress on Electron Tomography (4 ICET) was held on Nov $5{\sim}8$, 2006, at San Diego. The program dealt instrumentation, reconstruction algorithm, visualization/quantitative analysis and electron tomographic presentation of biological specimen and nano particles. 1 have summarized oral presentations and analyzed the posters presented on the meeting. Cryo-electron microscopic system was popular system for ET and followed conventional transmission electron microscopic system. Cultured cell line and tissue were most popular specimens analyzed and microorganisms including bacteria and virus also constituted important specimens. This analysis provides a current state of art in ET research and a guide line for practical application of ET and further research strategies.

Computer Vision System for Analysis of Geometrical Characteristics of Agricultural Products and Microscopic Particles(II) -Algorithms for Geometrical Feature Analysis- (농산물 및 미립자의 기하학적 특성 분석을 위한 컴퓨터 시각 시스템(II) -기하학적 특성 분석 알고리즘-)

  • Lee, J.W.;Noh, S.H.
    • Journal of Biosystems Engineering
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    • v.17 no.2
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    • pp.143-155
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    • 1992
  • The aim of this study is to develop a general purpose algorithm for analyzing geometrical features of agricultural products and microscopic particles regardless of their numbers, shapes and positions with a computer vision system. Primarily, boundary informations of an image were obtained by Scan Line Coding and Scan & Chain Coding methods and then with these informations, geometrical features such as area, perimeter, lengths, widths, centroid, major and minor axes, equivalent circle diameter, number of individual objects, etc, were analyzed. The algorithms developed in this study was evaluated with test images consisting of a number of randomly generated ellipsoids or a few synthesized diagrams having different features. The result was successful in terms of accuracy.

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Analysis of Cutting Mechanism by Image Processing on Micro-Cutting in SEM (전자현미경내 마이크로 절삭의 화상처리에 의한 절삭 기구 해석)

  • 허성중
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.12 no.3
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    • pp.89-95
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    • 2003
  • This research analyzes the cutting mechanism of A1100-H18 of commercially pure aluminum by image processing in SEM(Scanning Electron Microscope) for the measurement of strain rate distribution near a cutting edge in orthogonal micro-cutting. The distribution is measured using various methods in order. The methods are in-situ observations of cutting process in SEM, inputting image data, a computer image processing, calculating displacements by SSDA(Sequential Similarity Detection Algorithm) and calculating strain rates by FEM. The min results obtained are as follows: (1)It enables to measure a microscopic displacement near a cutting edge. (2) An application of this system to cutting process of various materials will help to make cutting mechanism clear.

The Study of Hanji and Washi Fiber Orientation using Image analysis (Image analysis에 의한 한지와 화지의 섬유 배향성 연구)

  • Han, Yoon-Hee;Enomae, Toshiharu;Isogai, Akira
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2006.11a
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    • pp.89-96
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    • 2006
  • To estimate the manufacturing district and generation of ancient paper as a cultural property, fiber orientation is one of the criteria. Image analysis using fast Fourier transform with suitable modifications was demonstrated to be an effective means to determine angle and intensity of fiber orientation as a nondestructive method. Binarization process of microscopic images of paper surface and precise calculation for average Fourier coefficients as an angular distribution by linear interpolation were newly introduced in the procedures to improve the accuracy. This analysis method was applied to digital optical micrographs of paper surfaces. Korea and Japanese traditional hand making papers were well distinguished. Korea and Japanese papers made in the traditional ways showed its own characteristic orientation behavior in accordance with the motion of a bamboo wire.

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