• Title/Summary/Keyword: Microscopic evaluation

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Arthroscopic Evaluation for Endoscopic ACL Reconstruction Using a Multi-stranded Hamstring Tendon Graft and EndoButton (슬괵 건을 이용한 전방십자인대 재건술후 2차 관절경적 평가)

  • Lee, Kwang-Won;Lee, Byeong-Ki;Ryu, Chang-Soo;Keum, Teok-Seop;Choy, Won-Sik;Kim, Joon-Sik
    • Journal of the Korean Arthroscopy Society
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    • v.2 no.2
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    • pp.124-128
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    • 1998
  • From Feb. 1996 to Feb. 1997. among sixty-four patients of anterior cruciate ligament(ACL) reconstruction using multi-stranded hamstring tendon and EndoButton fixation, twenty-five patients of volunteer, 15 men and 10 women, from 16 to 50 years of age(mean 27.6 years) underwent arthroscopic evaluation. Arthroscopic evaluation was routinely performed one year after surgery along with an examination of stability. The purpose of this study was to arthroscopically evaluate the morphological changes that occurred in hamstring tendon autograft one year after ACL reconstruction. Both of the tendon graft and the adjacent fibrous tissues were observed for gross appearance and physical properties with probing and response to passive anterior translation. Biopsy samples were taken from the surrounding fibrous tissue and the tendon graft. Grossly, we found a well-defined ligamentous structure with good tension tested with a probe. A common finding was that the reconstructed ligaments were embedded in a layer of the synovium, and blood vessels could be seen on the anterior surface of the ligament. The biopsy tissue was placed in formalin solution and subjected to histological preparation by hematoxylin and eosin staining method. The hamstring tendon graft appeared histologically normal under light microscopic finding. There were a few vessels located between existing collagen bundles, and the vessels had an orderly pattern. The surrounding fibrous tissue envelope was covered with synovium. We pleased with preliminary findings in our evaluation.

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Subchronic Oral Dose Toxicity of Freeze-dried Powder of Allomyrina dichotoma Larvae

  • Noh, Jung-Ho;Yun, Eun-Young;Park, Heejin;Jung, Kyung-Jin;Hwang, Jae Sam;Jeong, Eun Ju;Moon, Kyoung-Sik
    • Toxicological Research
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    • v.31 no.1
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    • pp.69-75
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    • 2015
  • The objective of this study was to investigate the toxicological information of freeze-dried powder from Allomyrina dichotoma (A. dichotoma) larvae as a food ingredient. The powder, suspended in distilled water, was administered once daily by oral gavage to four groups of Sprague-Dawley (SD) rats at dose levels of 0 (vehicle control), 250, 850, and 2500 mg/kg/day. After 13 wks of repeated administration, the standard toxicological parameters such as mortality, clinical signs, body weight, food consumption, ophthalmologic examination, clinical pathology, organ weights and macro/microscopic examination were applied for assessment of general toxicity. In addition, serum IgE and histamine levels were determined to evaluate allergenicity. The freeze-dried powder from A. dichotoma larvae did not produce treatment-related changes or findings in any toxicological parameters in either sex of any dosed groups except for slight increases in serum histamine levels at 2500 mg/kg/day. The changes were considered not to be adverse since the magnitude was minimal. In conclusion, the NOAEL (No Observed Adverse Effect Level) of the freeze-dried powder from A. dichotoma larvae was determined to be 2500 mg/kg/day or more in both sexes of SD rats and it is considered a candidate to be edible material.

Quality Characteristics of Beef Jerky made with Beef meat of various Places of Origin (쇠고기 원산지 차이에 의한 육포의 품질 특성)

  • Park Ji-Hyoung;Lee Kyung-Hee
    • Korean journal of food and cookery science
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    • v.21 no.4 s.88
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    • pp.528-535
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    • 2005
  • This paper aims to compare and evaluate the quality of beef jerky made with Korean beef meat, Korean beef cattle and imported beef meat from Australia and New Zealand through sensory evaluation, measurement of hardness, thickness and contents of the moisture and crude lipid, and microscopic texture observation (SEM). According to the sensory evaluation, beef jerky made with Korean beef meat recorded the highest score in overall acceptability; New Zealand beef meat, the lowest. The preference of beef jerky made with Korean beef meat was shown in the appearance, smell, and texture, and especially in the taste. Whereas Korean beef jerky scored highest in color, glaze and palatability, New Zealand beef meat showed the lowest score. Korean beef meat showed the lowest in hardness, but New Zealand beef meat showed the highest as shown in the result of technical evaluation through rheometer. The evaluation through colormeter concluded that New Zealand beef meat had the highest scores in L and a values, but that Korean beef meat showed the lowest score. When it comes to the color difference compared with Korean beef meat, New Zealand beef meat showed the biggest difference. Although the moisture contents of jerky made with different beef meat showed little difference, the content of the crude lipid of jerky made with Korean beef meat and Australian beef meat was lower than that of Korean beef cattle and New Zealand beef meat. When the beef jerky is dry, fat contained in the beef meat is dissolved, which causes the glaze on the outside. Scanning electron micrographs showed that Korean and Australian beef jerky had larger gaps than Korean beef cattle and New Zealand beef jerky. It was concluded that this affected the hardness of beef jerky.

Taxonomic Re-evaluation of Megacollybia Species in Korea

  • Kim, Chang Sun;Jo, Jong Won;Kwag, Young-Nam;Han, Jae-Gu;Shrestha, Bhushan;Sung, Gi-Ho;Han, Sang-Kuk
    • Mycobiology
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    • v.42 no.1
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    • pp.22-26
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    • 2014
  • The diversity of the genus Megacollybia in Korea was examined based on morphological observation and analysis of molecular data. Currently, the genus is consisted of nine species with a global distribution. However, only M. platyphylla has been reported in the floral survey of Korea. During our re-evaluation of the taxonomic diversity of Megacollybia in Korea, six Megacollybia specimens collected in 2012 were identified based on internal transcribed spacer (ITS) sequences and morphology. Here, we report two Megacollybia species, M. clitocyboidea and M. marginata, as newly recorded species from Korea. The microscopic features of the two species are provided in this study. To our knowledge, this is the first phylogenetic analysis of Korean Megacollybia species.

EFFECTS OF EARLY FUNCTIONAL LOADING ON BONE FORMATION AROUND TITANIUM PLASMA SPRAYED IMZ IMPLANTS IN DOGS (성견에서 Titanium Plasma Sprayed IMZ 임플란트에 가해진 조기 기능하중의 골유착 및 골형성에 미치는 영향)

  • Jeon, Young-Sik;Han, Dong-Hoo
    • The Journal of Korean Academy of Prosthodontics
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    • v.33 no.3
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    • pp.550-568
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    • 1995
  • The purpose of this study was to observe the effects of early functional on bone formation around titanium plsama sprayed IMZ implants. 15 IMZ implants were inserted on the mandibular premolar areaa in 5 mondgrel dogs. The inplants were divided into 4 groups according to it’s loading schedule : Temporary abutments were connected for functional loading 6weeks, 9weeks, and 12weeks after implants installation in group I, II, and III, respectively. Abutments were not connected in the control group. After polyfluorochrome sequential labeling, all animals were sacrificed and bone sections includig implants and the 1st. molar were removed and processed for histologic evaluation. From light and fluorescence microscopic evaluation as well as histomorphometric and EPMA analysis, following results were obtained : 1. Light microscopically, the osteoid tissues were observed much in the compact bone of unloaded group, and cancellous bone formation observed more in early loaded group. 2. Calcification and remodeling of bone took place faster in loaded group, especially in 6 weeks group. 3. Moderate to severe marginal bone resorption was observed in 6 weeks group, mainly on buccal crest. 4. There was no difference in the percentages of bone-to-metal contact among the experimental groups. 5. In quantitative analysis using EPMA, there was no difference in the content of calcium and phosphorus among the experimental groups, regardless of distance.

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Interfacial and Nondestructive Evaluation of Single Carbon Fiber/Epoxy Composites by Fiber Fracture Source Location using Acoustic Emission (Acoustic Emission 의 섬유파단 Source Location을 이용한 Carbon Fiber/Epoxy Composites의 계면특성 및 비파괴적 평가)

  • Kong, Jin-Woo;Kim, Jin-Won;Park, Joung-Man;Yoon, Dong-Jin
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2001.10a
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    • pp.116-120
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    • 2001
  • Fiber fracture is one of the dominant failure phenomena to determine total mechanical properties in composites. Fiber fracture locations were measured by optical microscopic method and acoustic emission (AE) as functions of matrix toughness and surface treatment by the electrodeposition (ED), and then two methods were compared. Two AE sensors were attached on the epoxy specimen and fiber fracture signals were detected with elapsed time. The interfacial shear stress (IFSS) was measured using tensile fragmentation test and AE system. In ED-treated case, the number of the fiber fracture measured by an optical method and AE was more than that of the untreated case. The signal number measured by AE were rather smaller than the number of fragments measured by optical method, since some fiber fracture signals were lost while AE detection. However, one-to-one correspondence between the x-position location by AE and real break positions by optical method was generally established well. The fiber break source location using AE can be a valuable method to measure IFSS for semi- or nontransparent matrix composites nondestructively (NDT).

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Pharmacognostical Evaluation of Trachyspermum roxburghianum (DC) Craib Fruits

  • Verma, Nitin;Khosa, R.L.
    • Natural Product Sciences
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    • v.17 no.1
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    • pp.45-50
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    • 2011
  • Sophisticated modern research tools for evaluation of medicinal plants are available but microscopic methods are one of the simplest and cheapest methods to establish the identity of the source materials. Pharmacognostical investigation of the dried, powdered and anatomical sections of the fruits of Trachyspermum roxburghianum (DC) Craib was carried out to determine its macro and microscopical characteristics along with its physical constants. Externally, the fruits, yellowish or greenish brown in colour are elongated, elliptical, slightly curved, prominently ridged and longitudinal. As seen in transectional views of the fruits from Trachyspermum roxburghianum, the mericarp has concave sides called commissural surfaces and a convex outer side called the dorsal surface. The mericarp has three primary ridges alternating with two secondary ridges on the dorsal side. On the commissural side, there are two primary ridges which are lateral in position and two secondary ridges in the commissural side. The seed is attached to the pericarp by a short stalk called a raphe. Circular, four-lobed calcium oxalate crystals are fairly abundant in the endosperm. Phytochemical studies revealed the presence of phenolic compounds, triterpenoids, proteins and sugars. The pharmacognostical profile of the fruits will assist in standardization for quality, purity and sample identification.

TSM Strategies and Evaluation of Traffic Performance - Special Reference to a Case Study of Reversible Lane Technique. (TSM전략과 효율측정-일례연구를 중심으로)

  • 도철웅
    • Journal of Korean Society of Transportation
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    • v.4 no.1
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    • pp.3-11
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    • 1986
  • One important element of a systematic approach to the management and control of the flow of people over an urban street network is the monitoring and evaluation of system performance. The nature of TSM strategies that, in part, differentiates them from traditional long-range transportation improvement alternatives is that they are less costly, are more quickly implemented and modified, and are often oriented toward sub-area problems which must be addressed at a more microscopic level of analysis. These factors suggest that pre-implementation evaluations of alternative TSM actions will often have to rely on quick-turn around, manual methods of analysis to guide the choice of which management action should be implemented. This paper was prepared to focus on the definition and importance of TSM, specifically associated with monitoring and evaluating traffic performance in the context of TSM startegies. A simple case study of reversible lane technique was presented. The purposes of the case study is to illustrate the methodology of evaluating TSM strategies and demonstrate to identify the benefit from the reversible lane technique, which may otherwise be overlooked in real world. Applying the reversible lane technique to Sam-Il elevate highway, it was found to be a very promising low cost alternative to reduce total travel time(or delay) and fuel consumption.

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Study on Hydrogen Effect in TIG Welded Stainless Steel (TIG 용접된 스테인리스강의 수소영향에 대한 연구)

  • Lee, Jin-Kyung;Lee, Sang-Pill;Bae, Dong-Su;Lee, Joon-Hyun
    • Journal of Power System Engineering
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    • v.20 no.6
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    • pp.58-63
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    • 2016
  • A stainless steel has high corrosion resistance because of nickel in material, so it is used as materials for transportation and storage of hydrogen. In this study, TIG(tungsten ingot gas) welding was carried out on the stainless steel using the storage vessel of hydrogen. The microscopic structures at each region of TIG welded material such as HAZ, weld and base metals using optical microscope were observed. And the damage behavior of stainless steel that underwent the hydrogen charging using nondestructive evaluation was also studied. Ultrasonic test, which is the most generalized nondestructive technique, was applied to evaluate the relationship between the ultrasonic wave and mechanical properties at each zone of TIG welded stainless steel. The velocity and attenuation coefficients of ultrasonic wave didn't show a remarkable difference at each region of welded stainless steel. However, the attenuation coefficient was the highest at the weld zone when hydrogen charged stainless steel. In addition, acoustic emission test was also used to study the dynamic behavior of stainless steel experienced both hydrogen charging and weld. Lots of AE event at elastic region of stress-strain curve were occurred both the hydrogen charged specimen and the free specimen.

An investigation on the infection of Encephalitozoon cuniculi and pathological changes in laboratory rabbits (실험용 토끼에서 Encephalitozoon cunuculi의 감염과 병리학적 변화)

  • Yoon, Byong-Ill;Lee, Sang-Koo
    • Korean Journal of Veterinary Pathology
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    • v.1 no.1
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    • pp.66-71
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    • 1997
  • Eighteen NZW rabbits used for local skin irritation study were examined grossly and microscopically for natural infection with Encephalitozoon cuniculi. For microscopic tissue evaluation histochemical techniques such as PAS Gram iron hematoxylin and HE stain were used. Although rabbits in the study had no abnormal clinical signs 7/18(38.9%) animals were microscopically infected with E. cuniculi. The affected rabbits had gray-whitish and depressed approximately 0.1∼0.6 cm diameter lesions in the kidneys. All other organs examined were grossly unremarkable. Histopathologically however in addition to segmental interstitial nephritis focal lymphocytic myocarditis and granulomatous inflammatory reaction in portal areas of the liver multifocal granulomatous foci with vasculitis were present in the brain kidney and lungs. Aggregates of minute oval organisms were observed in brain and kidney sections frequently within the granulomatous foci and sometimes without any inflammatory reaction particularly in the renal tubules. in histochemical stains the organisms were gram positive stained with iron hematoxylin and had PAS positive granule at one pole. They measured approximately 1.5×2.5μm consistent with E. cuniculi. Histochemical characterization is important to differentiate E. cuniculi from other common protozoal infection such as Toxoplasma gondii. This study demonstrate the importance of subclinical. E. cunuculi infection and the associated histological alterations may interfere with tissue evaluation in toxicologic studies.

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