• Archives of Plastic Surgery
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• 제50권6호
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• pp.610-614
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• 2023

Necrotizing fasciitis is an uncommon yet fatal soft tissue infection. Current recommended treatment includes antibiotics with repeat surgical exploration and wound debridement followed by reconstruction. In burn patients, the Meek micrograft has demonstrated a higher true expansion ratio, faster reepithelialization rate, more resilient toward infection, and reduced risk of graft failure as compared with meshed graft. To our best knowledge, the use of Meek micrografting technique in reconstruction of postdebridement wounds of necrotizing fasciitis has not been reported. Hereby, we present a case of a 57-year-old gentleman who was referred to us for wound reconstruction after surgical debridement of Fournier's gangrene and extensive necrotizing fasciitis involving the anterior abdomen and bilateral femoral region. Meek micrografting technique was used to reconstruct the anterior abdomen as the wound bed was large. Although the graft was complicated with a small area of localized infection, it did not spread across the entire graft and was successfully treated with topical antibiotics and regular wound dressing. In our case, wound reconstruction using Meek micrografting technique in a patient with extensive necrotizing fasciitis was successful and showed positive outcome. Therefore, we suggest further studies to be conducted to investigate the applications and outcomes of the Meek micrografting technique, especially in patients with extensive wound bed and limited donor site availability.

• 한국산림과학회지
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• 제82권3호
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• pp.254-259
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• 1993

이병묘로부터 마이코플라즈마 무병주의 생산법을 확립하기 위하여 대추나무 시험관묘의 액아에서 생장점을 채취하여 기내접목을 실시하였다. 대목으로는 기내에서 발아시킨 대추나무 실생묘의 배축을 사용하였다. 접수중 10%(30점 중 3점씩 2회)가 정상적으로 생장하였고 계속적인 증식과 발근이 가능하여 유식물체로 분화되었다. 마이코플라즈마의 존재여부를 전자현미경과 형광현미경 검경방법을 이용하여 검경한 결과, 접수로 사용한 시험관묘에서는 마이코플라즈마가 관찰되었으나 접목묘에서는 관찰되지 않았다. 이로써 마이코플라즈마를 보균한 시험관묘의 생장점을 접수로 한 기내 미세접목을 통하여 건전묘 생산의 가능성을 보여주었다.

• Plant Biotechnology Reports
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• 제2권2호
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• pp.137-143
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• 2008

Production of Indian citrus ringspot virus (ICRSV)-free plants from an infected plant of kinnow mandarin (Citrus nobilis Lour ${\times}$ C. deliciosa Tenora) is reported. The shoot apices of different sizes (0.2-1.0 mm) excised from the ICRSV-infected plant were micrografted onto decapitated rootstock seedlings of rough lemon (C. jambhiri). Micrograft survival depended on the size of shoot apex and the sucrose concentration of the culture medium. Increase in scion size from 0.2 to 0.7 mm resulted in an increase in micrografting success rate from 30.55 to 51.88%. Further, micrograft survival obtained with 0.2 mm was improved from 30.55 to 38.88% by increasing sucrose concentration in the culture media from 5 to 7.5%. The micrografted plants were tested for ICRSV using ELISA and RT-PCR. All plants raised from 0.2-mm scion were found negative with both ELISA and RT-PCR whereas only 20% of the ELISA negative plants raised from 0.3-mm scion were found negative for ICRSV with RT-PCR. The outcome of this research is the successful establishment, acclimatization and virus testing of micrografted plants.

• 생명과학회지
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• 제23권2호
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• pp.267-272
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• 2013

본 연구는 바이러스에 감염된 감귤나무로부터 열처리와 경정접목을 병행 처리하여 바이러스를 제거코자 수행하였다. '세토카' 교잡종을 포함한 6품종을 재료로서 사용하였으며 이를 조사된 모든 개체에서는 외관적으로 바이러스 감염 증상이 관찰되지 않았으나 TAS-ELISA 검정을 통해 전원 CTV에 감염되어 있음이 확인되었다. '세토카'를 대상으로 특정 온도 시험을 수행한 결과, 주 야간 $40^{\circ}C$ 온도 조건에서 높은 비율로 바이러스가 제거되었다. 항혈청법으로 할 수 있었으며, 그 처리에서 획득된 무균의 신초를 이용하여 기내 파종된 탱자 대목에 경정 접목하였다. 경정 접목된 모든 개체에서는 바이러스 병징이 발견되지 않았고 TAS-ELISA와 SDV 크로마토법에 의해서도 무균이 확인되었다. CTV, SDV와 CTLV를 대상으로 한 RT-PCR 검사를 통해서도 극소량의 바이러스조차 발견되지 않았다. 따라서, 열처리와 경정접목을 통해 '세토카', '삼다조생', '풍광', '부지화' 및 '에히메카시 다이28고' 품종에서 총 11개의 무균의 식물체를 획득하였다.

• 한국자원식물학회지
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• 제31권6호
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• pp.684-694
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• 2018

This study describes the successful establishment of a cryopreservation protocol for Citrus limon cultivars: 'Frost Eureka limon' and 'Cook Eureka limon', using a droplet-vitrification method. The shoot tips that were excised from in vitro grown seedlings of the two cultivars were preserved in liquid nitrogen (LN) and successfully regenerated into whole plants. Excised shoot tips were pre-cultured for 1 or 2 days in 0.3 M and 0.5 M sucrose solutions at $25^{\circ}C$ and incubated in a loading solution (LS) composed of 17.5% glycerol + 17.5% sucrose in Murashige and Skoog (MS) medium for 40 min at $25^{\circ}C$. Prior to direct immersion in LN for 1 h, the shoot tips were dehydrated with plant vitrification solution 2 (PVS2) at $0^{\circ}C$ or PVS3 at $25^{\circ}C$. The frozen shoot tips were re-warmed and unloaded with 1.2 M sucrose in $\text\tiny{^1/_2}$ MS for 30 min at $25^{\circ}C$. Shoot tips were post-cultured overnight on survival medium and then micrografted onto 'trifoliate orange' (Poncirus trifoliate (L.) Raf. seedling rootstocks for recovery and to produce whole plants. The highest regrowth rates were 53.5% and 50.3% for cryopreserved shoot tips of 'Frost Eureka limon' and 'Cook Eureka limon', respectively, when pre-cultured in 0.3 M and 0.5 M sucrose concentrations in a sequencing manner, with LS and treated with PVS2 for 60 min at $0^{\circ}C$. We also investigated whether the ammonium ion concentration on post-culture medium affected the viability of the cryopreserved Citrus shoot tips. The viability of cooled samples, following culturing on woody plant media (WPM) containing $\text\tiny{^1/_4}$ ammonium nitrate overnight before micrografting, was the highest (70.3%) in 'Frost Eureka limon'. The study described here is a cost-effective and safe method to conserve Citrus fruit cultivars, for the improvement and large-scale multiplication of fruit plants and for breeding disease resistance.