• Archives of Reconstructive Microsurgery
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• v.6 no.1
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• pp.1-8
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• 1997

Recently prostaglandin $E_1(PGE_1)$ has been shown to ensure flap survival by producing vasodilation of the peripheral vessels and platelet disaggreation. However, direct observation and detailed quantitative studies of the effects of $PGE_1$ on the cutaneous microcirculation have not been reported. In the present study, we investigated cutaneous microcirculatory changes in the rabbit ear chamber(REC) with an intravital microscope following intravenous administration of $PGE_1$. The results obtained in this study indicate that $PGE_1$ administered intravenously at a rate of 200ng/kg/min might act directly on the vessels and cause dilatation of metarterioles and capillaries without affecting vasomotion and systemic blood pressure. Clinically in order to evaluate the effect of an intravenous administration of $PGE_1$ on the cutaneous microcirculation, cutaneous blood flow, skin temperature and transcutaneous $Po_2$ in the pedicle or free flap of operated patients were evaluated by the combination of several measurements following the administration of $PGE_1$. The present study suggests that improvement of cutaneous microcirculation by $PGE_1$ may enhance the survival rate of flap or replantation. Both vessel arterial ischemia and venous congestion are main factors of tissue necrosis in the flap surgery. Vasodilatory or antithrombotic agents have been used in salvage of flap necrosis. However, the therapeutic effects of those drugs are still not well elucidated. Recently prostaglandin $E_1(PGE_1)$ has been shown to ensure flap survival by producing vasodilatation of the peripheral vessels and platelet disaggregation[1-3]. Emerson and sykes[4] have obtained significant improvement in the flap survival in the rat using $PGI_2$. Suzuki et al.[5] have reported prolonged flap survival length by using $PGE_1$ in the rabbit and concluded that $PGE_1$ improved the microcircuration in the flap. However, direct observation and detailed quantitative studies of the effects of $PGE_1$ on the cutaneous microcirculation have not been reported. In the present study, we investigated microcirculatory changes in the rabbit ear chamber[6,7] with an intravital microscope following intravenous administration of $PGE_1$.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.1
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• pp.85-90
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• 2010

Several studies have demonstrated that ginseng-berry extract has several beneficial properties, including anti-inflammatory, antioxidant, and vasodilation properties. Ginseng-berry extract has also been shown to have the great potential against skin aging. Its beneficial mechanism against skin aging, however, has not been examined in detail. Also, the effects of ginseng-berry extract on microcirculation and skin cellular responses have not been investigated. Inhibition of skin microcirculation is the primary cause of many adverse biological effects, which is responsible for the skin aging and darkening. We investigated the beneficial effects of ginseng-berry extract on blood circulation, transcutaneous oxygen pressure in vivo model and also on skin microcirculation, cellular response and skin brightening effect in clinical trial. We found that oral administration of ginseng-berry extract markedly increased blood flow rate and transcutaneous $O_2$ pressure, but decreased transcutaneous $CO_2$ pressure. Also, it improved skin tone on cheeks, as is skin brighteness. These results suggest that ginseng-berry extract is a potent candidate for the treatment of skin aging and brightening by improving skin microcirculation.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1923-1929
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• 2003

The present study investigated the microcirculation of blood in the left common artery (LCCA). In order to develop a mathematical model for microcirculation in LCCA, the present study adopted preexisted set of measured morphological data on anatomy, mechanical properties of the coronary vessels, viscosity of blood, the basic laws of physics, and the appropriate boundary conditions. In this study, the statistical distribution of blood pressure, blood flow, and blood volume in the LCCA were determined based on the anatomical branching pattern of the coronary arterial tree and the statistical data of blood vessel dimensions. Our calculations were in good agreement with the previous studies. The present results showed that the mean longitudinal pressure drop profile was function of the vessel order numbers. It was found that the normalized pressure drop was a logarithmic function of the compliance.

• Journal of Veterinary Clinics
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• v.34 no.4
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• pp.249-254
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• 2017

The cutaneous microcirculation plays a role in various physiological processes and pathological conditions. Two non-invasive methods were used in this study to obtain reference values for cutaneous microcirculation in intact male beagles. Twenty intact male beagles were used. The experimental environments were standardized. Laser Doppler flowmetry was used to measure cutaneous blood flow, and an infrared thermometer was used to measure cutaneous temperature. The blood flow and temperature were measured from the right side of the subjects at 20 cutaneous sites. Based on the laser Doppler flowmetry, the region with the highest blood flow was the periocular region that with the lowest was the forelimb foot pad. In addition, the standard deviation of the chest wall was the highest while that of the periocular region was the lowest. For skin temperature, the inguinal region had the highest mean skin temperature and the forelimb foot pad had the lowest. The correlation coefficient between the two methods was 0.72. Similar to a previous study, the values derived from repeated measurements at the 20 regions are reproducible and can contribute to research. Compared to the results of a previous study, the temperatures of the two smallest skin regions were the same; however, no specific trend was observed. The correlation coefficient between the two methods was significantly comparable, and this good correlation can reduce their limitations and variables complementarily. In addition to possible use in human studies, accumulated resources on measurements of skin blood flow in the future will potentiate its use in the veterinary medicine field.

• Journal of Biomedical Engineering Research
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• v.43 no.4
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• pp.193-198
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• 2022

Contrast enhanced magnetic resonance imaging using gadolinium-based contrast agent (GBCA) is a very useful in vivo technique to visualize the inner ear pathology including endolymphatic hydrops. Although systemic intravenous (IV) administration can visualize the perilymph space, the visualization was possible by indirect passage of contrast agent through blood-perilymph barrier. All animal experimental procedures were performed under anesthesia with 5% isoflurane. Lipopolysaccharide (LPS) was instilled into the left tympanic cavity through the tympanic membrane using a sterile 27gauge needle to induce hydrops model. Tucker-Davis Technologies system was used to measure Auditory Brainstem Responses (ABRs). For intracerebroven-tricular (ICV) administration, 25 µmol of GADOVIST (Bayer, Berlin, Germany) was used and diluted GADOVIST injection was 10 µl. MR imaging was acquired with a 9.4 Tesla MRI scanner. Transmit-receive volume coil with 40 mm inner diameter and 75 mm out diameter was used. ICV administration well demonstrated the strong enhancement along the cerebrospinal fluid (CSF) microcirculation pathway including CSF fluid in the subarachnoid space and CSF space of the inner ear structures. On the other hand, IV administration showed no contrast enhancement along the CSF microcirculation pathway and showed weak enhancement in the inner ear structures. In case of rat hydrops model, ICV administration showed that the reduced contrast enhancement in the perilymph space of the hydrops induced inner ear compared to the contrast enhancement in the perilymph space of the normal inner ear. New systemic ICV administration method provide contrast enhancement of GBCA in the inner ear through CSF microcirculation pathway.

• 대한미세수술학회:학술대회논문집
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• 1996.10a
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• pp.183.1-183.1
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• 1996

• 대한미세수술학회:학술대회논문집
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• 1995.11a
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• pp.83.2-84
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• 1995

• Restorative Dentistry and Endodontics
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• v.21 no.1
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• pp.375-384
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• 1996

The purpose of this study was to investigate the functional involvement of sympathetic nerve in the control of the microcirculation in the dental pulp with the aim of elucidation of the involvement of neuropeptides and sympathetic nerve in neurogenic inflammation. Experiments were done on the 7 cats anesthetised with sodium pentobarbital, and sympathetic nerve to the' dental pulp was stimulated electrically (10 Hz, 4 V, 1.5 ms, 3.5 mins). Ana-adrenoceptor antagonist phentolamine and a neuropeptide Y antagonist D-myo-inositol-1,2,6-trisphosphate (PP56) were injected close intra-arterially into the dental pulp without changing the systemic blood pressure. The probe of laser Doppler flowmeter was placed on the buccal surface of ipsilateral canine teeth to the stimulation, and pulpal blood flow was measured. Stimulation of the sympathetic nerve decreased pulpal blood flow by $55.24{\pm}7.74\;%$ (mean${\pm}$SEM, n = 13). Stimulation of the sympathetic nerve following the injection of the ${\alpha}$-adrenoceptor antagonist phentolamine ($0.1{\mu}g$/kg) caused decrease of pulpal blood flow by $14.35{\pm}3.43%$ (mean${\pm}$SEM, n=5). Phentolamine attenuated the sympathetic nerve-induced pulpal blood flow decrease by $74.02{\pm}9.32%$ (mean${\pm}$SEM) Stimulation of the sympathetic nerve following the injection of the neuropeptide Y antagonist PP56 (2.3 mg/kg) caused decrease of pulpal blood flow by $30.64{\pm}7.92%$ (mean${\pm}$SEM, n=6). PP56 attenuated the sympathetic nerve-induced pulpal blood flow decrease by $44.37{\pm}11.01%$ (mean${\pm}$SEM). These data provide evidences of the co-contribution of nerepinephrine and neuropeptide Y on the sympathetic nerve-induced vasoconstriction in the feline dental pulp. In addition, they show functional evidences that sympathetic nerve plays an active role in controlling the microcirculation of the dental pulp.

• The Korea Journal of Herbology
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• v.22 no.2
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• pp.45-50
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• 2007

objectives: We investigated the moisturizing and cooling effects of the cosmetic product with Scrophularia Buergeriana, Poria Cocos, Lonicera Japonica, Portulaca Oleracea and Ginko Biloba extract on human skin by using non-invasive instrument. Methods: We measured physiological effects after volunteers(female, n=5) applied the cosmetic product containing the extract of oriental herbs. The forearm skin moisturization was measured with $Corneometer^{\circledR}$ CM825 after 15min, 3hr, and 6hr. The forearm skin surface roughness was with Scanning electron microscopy after 15min, and 6hr. The face skin temperatures cooling and microcirculation effects were measured with ThermaCAMTM PM525 and LDl-Periscan PlM II after 15min, and 30min. Results: The forearm skin moisturization was imcreased 108% after 15min. The forearm skin surface roughness was decreased significantly after 15min. The face skin temperatures were cooling down to $30.7^{\circ}C$. The face skin microcirculation effect was normalized time-dependently. Conclusions: We concluded that cosmetic product containing the extract of Scrophularia Buergeriana, Poria Cocos, Lonicera Japonica, Portulaca Oleracea and Ginko Biloba had a moisturization effect and improving the surface roughness, temperature and microcirculation on the human skin.

• Journal of the KSME
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• v.55 no.3
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• pp.38-41
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• 2015

이 글에서는 무세포 경계층(red blood cell free layer)의 생리학적 중요성 및 미세순환(microcirculation)에서 이루어지는 가스교환 예측을 위한 수치해석모델(numerical simulation) 개발을 소개하고자 한다.