• 한국수산과학회지
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• 제55권4호
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• pp.431-436
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• 2022

For the safety assessment of microbiological and chemical hazards in katsuobushi, fifteen samples of katsuobushi were purchased from supermarkets. The contamination levels of total viable bacteria, coliforms, Escherichia coli, and nine pathogenic bacteria [Staphylococcus aureus, Salmonella spp., Listeria monocytogenes, Bacillus cereus, Vibrio parahaemolyticus, Clostridium perfringens, Enterohemorrhagic E. coli (EHEC), Yersinia enterocolitica and Campylobacter jejuni/coli] were quantitatively or qualitatively assessed. Additionally, the heavy metals (total and methyl mercury) content, radioactivity (¹³¹ I, ¹³⁴ Cs⁺ and ¹³⁷ Cs) were quantitatively assessed. Microbial and chemical analyses were performed using standard methods in Korean food code. The contamination level of total viable bacteria was 2.70 (1.18-4.42) log CFU/g. Coliforms, E. coli and S. aureus were not detected in any samples. Other eight pathogenic bacteria were negative in all samples. The contamination levels of total and methyl mercury were 0.366 (0.227-0.481) and 0.120 (0.002-0.241) mg/kg, respectively. In addition, radioactivity was not detected in any samples. The results will be helpful in revitalizing domestic use and boosting exports of katsuobushi because the microbiological and chemical safety of katsuobushi has been assured. Furthermore, the results may be used as a basis for performing chemical and microbial risk assessments of katsuobushi.

• 한국식품위생안전성학회지
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• 제1권1호
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• pp.77-95
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• 1986

In 1962 the governing bodies of FAO and WHO approved the establishment of a joint FAO/WHO Food Standards Programme, the creation of a jointly sponsored body to be known as the Codex Alimentarius commission to implement the Programme. It can reasonably be claimed that the Commission has assumad the leading role in establishing internation food standards throughout the world. The Codex Committee of Food Hygiene has received much advice and assistance from other international organization which have been working in this field for a number of years. In particular, it has received valuable background documentation from the International Commission on Microbiological Specifications for Foods(ICMSF) which was set up by the International Association of Microbiological Societies(IAMS), and also from the International Organization for Standardization (ISO). Nevertheless, in spite of the information supplied by governments and research bodies in this field, microbiological standards have proved to be a highly controversial subject from the point of view of Codex standards. When it is decided to establish a microbiological standard for a food or class of foods, the following technical and administrative aspects must be considered: 1) The standard should be based on factual studies and serve one or more of the following objectives: (1) to determine the conditions of hygiene under which the food should be manufactured; (2) to minimize the hazards to public health; (3) to measure the keeping quality and storage potential of the food 2) The standard should be attainable under practicable operating and commercial conditions and should not entail the use of excessive heat treatment or the additions of extra preservatives. 3) The standard should be determined after investigation of the processing operation. 4) The standard should be as simple and inexpensive to administer as possible, the number of tests being kept to a minimum. 5) Details of methods to be used for sampling, examining and reporting should accompany all published microbiological standards. 6) In establishing tolerance levels for the permissible number of defective samples, allowance should be made for sampling and other variations due to differences in the laboratory methods. The following additional points should be kept in mind: 1) It is not satisfactory to establish one set of microbiological standards for a miscellaneous group of foods, such as“frozen foods”or“precooked foods”. 2) Microbiological standards should be applied first to the more hazardous types of food on the basis of experience of expected microbiological levels, taking into account variations in composition, processing procedures, and storage. 3) When a standard is established, there should be a definite relationship between the standard and the hazard against which it is meant to protect the public. 4) The sensitivity, reliability, and reproducibility of the sampling and analytical methods should be compared in different laboratories and the methods to be used should be specified in detail as part of the standard. 5) Tolerances should be included in the standard to account for inaccuracies of sampling and analysis. 6) Standards should be applied on a voluntary basis before compliance is made mandatory.

• 한국식품위생안전성학회지
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• 제10권2호
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• pp.103-109
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• 1995

Microbiological and organoleptic qualities of boiled anchovies were evaluated during storage for 8 months at different temperatures and packaging methods. Microbial populations of marketing samples were 106~107 CFU/g in aerobic bacteria, 103~106 CFU/g in yeasts/molds and 103~105 CFU/g in coliforms, respectively, which were the highest in retail sample, followed by in military goods and wholesale sample. Moreover, anchovies supplied for retail sale and military goods were contaminated with sanitary indicative microbes. The samples stored at ambient condition(15~33$^{\circ}C$, RH 5$0^{\circ}C$95%) lost their marketable quaity mainly due to microbial propagation prior to 6 months, irrespective of packaging methods, corrugated-cardboard box and laminated-film(nylon 15${\mu}{\textrm}{m}$/PE 100${\mu}{\textrm}{m}$). However, cooling(5~1$0^{\circ}C$) as well as well as freezing temperatures($\leq$-18$^{\circ}C$) following laminated-film packaging were effective for keeping the organoleptic qualities of stored anchovies up to 8 months. The population of yeasts and molds was shown the quality-indicative criteria for stored anchovies and their critical levels were 5.00 log counts/g, showing a higher negative-correlationship(r=-0.901) with changes in organoleptic quality.

• 한국식품위생안전성학회지
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• 제11권3호
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• pp.177-187
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• 1996

Time-temperature relationship and microbiological quality were assessed and critical control points were identified through hazard analysis during the phases of production in two different packaged meals (Dosirak) manufacturing establishments (A, B:Kim Pab). Microbiological tests on foods, equipments and utensils were done according to standard procedures and included total plate count, coliforms and fecal coliforms. The results of the study are summarized as follows : time-temperature control management was needed because time-temperature abuse more than 8 hours at dangerous temperature zone (5-6$0^{\circ}C$) was observed from pre-preperation to distribution phase; Poor sanitary practices of employees were observed in hand washing and using disposable gloves; Microbiological analysis results of equirpments and utensils showed possible cross-contamination risks when foods were contacted with them; Kim Pab needed thorough quality control because it included various mixed ingredients of cooked and uncooked and had many apportunities of cross-contamination either by equipments or hands through whole production processes.

• 한국식품위생안전성학회지
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• 제6권3호
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• pp.157-163
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• 1991

염장미역은 염농도 감소와 저장조건의 완화를 위한 연구의 일환으로서 2~4kGy의 감마선을 조사하고 ${30{\pm}2^{\circ}C와\;10{\pm}1^{\circ}C$에 저장하면서 미생물적, 관능적 품질을 평가한 결과는 다음과 같다. 염장미역의 변질에 관련된 원인 미생물은 호염성 세균과 효모로 확인되었으며, 2~4kGy의 감마선 조사는 초기의 미생물 수준을 1~2 log orders 감소시켰으나 저장 기간 중 증식에는 뚜렷한 영향이 없었다. 관능적 품질에서도 감마선 조사에 의한 미역조직의 연화현상으로 비조사군보다 낮은 기호도를 나타내어 저장 중 품질 안정성 향상에 별 효과가 없는 것으로 나타났다.

• 한국축산식품학회지
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• 제33권4호
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• pp.549-554
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• 2013

Food safety is important issue for consumers and recently the usage of food ingredients especially food preservatives are limited by regulations. However, some manufacturers use food preservatives instead of improving their hygienic production. Therefore, the levels of sodium benzoate and potassium sorbate of 147 vacuum packaged Kashar (fresh) cheese samples produced in Black Sea Region, Turkey were investigated and some microbiological properties were determined. Research results demonstrated that the production of vacuumed Kashar cheese in Black Sea Region was not standardized for all production periods depending on the microbiological properties. Coliform and E. coli counts detected in the cheese samples showed that necessary hygienic conditions were not provided for Kashar cheese production. Staphylococcus aureus was not determined in the cheese samples. The sodium benzoate and potassium sorbate were analyzed by HPLC-DAD. Potassium sorbate levels (69.39 mg/kg) of Kashar cheese samples were determined to be lower than the maximum permitted concentration of Turkish Food Codex. Although the utilization of sodium benzoate is prohibited by the Codex, the average level of sodium benzoate of cheese samples was detected to be 68.63 mg/kg. Sodium benzoate can be naturally occurred in fresh cheese at concentrations of up to 50 mg/kg.

• 한국식품조리과학회지
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• 제14권4호
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• pp.367-374
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• 1998

The purpose of this study was to evaluate the microbiological quality of, and to assure the hygienic safety of, the kimbap production in the university foodservice facilities in accordance with the HACCP (Hazard Analysis Critical Control Point) Program. The time-temperature relationship and the microbiological quality (specifically, total plate count and coliform bacteria count) were assessed to find the critical control point (CCP) during each of the production phases. The average of the daily longest duration time of the kimbap at the facilities was 23.4 hours in summer, while 29.6 hours in winter. In the purchasing phase of the raw materials, the microbiological quality of laver, fish paste, carrot and cucumber in summer was not at an acceptable level according to the standard set by the Natick research center, especially the number of TPC and the coliform level of laver was higher than the threshold level. In the refrigerator storage phase, the temperature of the carrot was 7.4$^{\circ}C$. This temperature is far exceeding the standard so that the microbiological counts was increased by the 2 log cycle during the average storage time of 17 hours or more. In the preparation phase, the temperature of the blanching is too low compared to the standard. In the holding phase before serving, its time-temperature relationship was out of the FDA food code standard both in winter and summer. In the sewing phase, the number of microbiological count was higher than the threshold level in summer while that in winter was up to standard. According to the Harrigan and McCance standard, the number of microbiological count of the utensils was higher than the threshold level in summer while that in winter was up to standard.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2006년도 International Meeting of the Microbiological Society of Korea
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• pp.58-60
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• 2006

• 한국식품영양과학회지
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• 제38권10호
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• pp.1457-1465
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• 2009

유치원 급식소 위생관리의 문제점을 파악하고 그에 대한 개선방안을 모색하고자 대구 경북지역 유치원 급식소를 대상으로 급식시설 설비 및 조리환경에 대한 미생물학적 위해분석을 2008년 5월에서 7월 사이에 실시한 결과는 다음과 같다. 조사대상 급식소에서 일반세균수와 대장균군수의 한계관리기준을 초과하여 관리되고 있다고 분석된 항목은 전처리용 칼, 조리용 칼, 조리용 도마, 전처리용 작업대, 조리용 작업대, 냉장고(안), 바트, 바구니, 식판 등 총 9개 항목이었다. 이 중 부적합율이 높은 항목은 조리용 도마(37.5%), 조리용 작업대(37.5%), 전처리용 작업대(33.3%)의 순이었다. 조사대상 유치원 급식소의 모든 검체에서 대장균과 리스테리 아균, 살모넬라균은 검출되지 않았으나, B 급식소의 조리용 고무장갑과 트렌치에서 황색포도상구균이 검출되었다. 조사대상 급식소의 공중낙하균 검사결과 일반세균수는 관리권고기준 이하로 적합한 수준이었고, 황색포도상구균은 모든 검사구역에서 검출되지 않았으나 한 곳의 급식소 조리실에서 진균수가 관리권고기준 이상으로 검출되었다. 위의 연구결과를 종합해볼 때 유치원의 급식관리자는 미생물학적 위해분석 결과 부적합하게 관리되고 있다고 분석된 전처리용 칼, 조리용 칼, 조리용 도마, 전처리용 작업대, 조리용 작업대, 냉장고(안), 바트, 바구니, 식판, 음용수, 조리용 고무장갑, 트렌치 등 총 12개 항목에 대한 위생관리를 우선적으로 개선할 필요가 있다고 판단된다. 또한 조사대상 급식소의 위생관리 현장심사 평가 결과 양호하다고 평가된 항목에 대한 미생물 검사 결과 한계관리기준을 초과하는 경우가 있었으므로 급식소의 위생관리 평가 시 현장심사와 함께 주요한 미생물 수준 부적합 항목에 대한 미생물 검사를 병행할 필요가 있다고 생각된다. 그리고 유치원 급식소의 위생관리 수준 개선을 위해 급식소 운영 특성을 고려한 위생관리지침서를 개발 보급하고 급식관리자는 최종 조리음식에 대한 교차 오염과 식중독균의 혼입을 방지하기 위해 위생관리지침서를 올바르게 적용해나가야 할 것이다. 이와 함께 우리나라 급식소 환경에 적용하기에 적합한 조리시설 설비의 미생물수준 및 공중낙하균에 대한 한계관리기준을 마련하기 위한 후속 연구도 활발하게 진행될 필요가 있다고 판단된다.

• Journal of Acupuncture Research
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• 제32권4호
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• pp.37-45
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• 2015

Objectives : The main aim of this study is to evaluate changes in the microbiological safety of pharmacopuncture exposed to room temperature for an elapsed period of time. Methods : The four most frequently used pharmacopuncture products were stored in syringes at room temperature at three different hospitals for 24 hours and 48 hours respectively, and they were compared with pharmacopuncture products stored in vials through a sterility and microbial limited test. Results : Storage forms and duration of exposure to room temperature did not show significant difference in bacterial or fungal contamination, which was confirmed by the sterility and microbial limited test. Conclusions : Pharmacopuncture products stored in syringes at room temperature for 24 hours and 48 hours demonstrated their safety in terms of lack of microbiological contamination.