• 한국식품위생안전성학회지
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• 제13권1호
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• pp.62-67
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• 1998

유부의 가공공정에 대한 위해 분석을 통한 위생관리 방안을 모색하기 위하여 가공공정별 시설.설비와 작업장 환경 및 원재료와 가공공정별 시료에 대한 미생물 변화와 오염원인을 분석하였으며, 포장유부를 $10^{\circ}C$에서 저장하면서 저장수명을 예측하고 이의 적절한 평가를 위한 품질지표를 도출코자 하였다. 가공공정의 효율성을 도모하고 최종제품의 저장수명을 개선하기 위해서는 가공공정 특히 사용용수, 침지, 성형, 절단, 유탕 이후의 공정에서의 작업장 환경과 시설.설비에 대한 위생 관리 대책과 침지와 성형공정에서의 미생물 증식 억제방안의 마련이 필요하였다. 유부의 저장수명을 예측하기 위한 품질지표로는 조직감 같은 관능지표와 육안적 곰팡이 발생 및 일반세균수가 매우 유용하게 이용될 것으로 판단되었으며, 유부의 저장수명은 최대 6일로 예측되었다. 이러한 연구는 유부의 가공조건 설정과 저장수명 평가에 대해 적절한 기초자료로 활용될 수 있을 것으로 기대된다.

• 한국환경보건학회지
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• 제39권1호
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• pp.32-42
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• 2013

Objectives: Cryptosporidium, a protozoan parasite, has been recognized as a frequent cause of waterborne disease due to its extremely strong resistance against chlorine disinfection. Although there has as yet been no report of a Cryptosporidium outbreak through drinking water in Korea, it is important to estimate the health risk of Cryptosporidium in water supply systems because of the various infection cases in human and domestic animals and frequent detection reports on their oocysts in water environments. Methods: This study evaluated the annual infection risk of Cryptosporidium in tap water using the quantitative microbial risk assessment technique. Exposure assessment was performed upon the results of a national survey on Cryptosporidium on the water sources of 97 large-scale water purification plants in Korea, water treatment efficacy, and daily unboiled tap water consumption. The estimates of the US Environmental Protection Agency on the mean likelihood of infection from ingesting one oocyst were applied for effect assessment. Results: Using probabilistic methods, mean annual infection risk of Cryptosporidiosis by the intake of tap water was estimated to fall within the range of $2.3{\times}10^{-4}$ to $1.0{\times}10^{-3}$ (median $5.7{\times}10^{-4}$). The risk in using river sources was predicted to be four times higher than with lake sources. With 0.5-log higher removal efficacy, the risk was estimated to be $1.8{\times}10^{-4}$, and could then be lowered by one-third. Conclusions: These estimations can be compared with acceptable risk and then used to determine the adequacy and priority of various drinking water quality strategies such as the establishment of new treatment technology.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1502-1509
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• 2015

The objectives of this study were to evaluate the effect of combined treatments (slightly acidic electrolyzed water (SAEW), ultrasound (US), or mild heat (60℃)) on the growth of Listeria monocytogenes and Salmonella enterica serovar Typhimurium in fresh-cut bell pepper, and the shelf-life and sensory quality (color and texture) were followed during storage at 4℃ and 25℃. An additional 0.65, 1.72, and 2.70 log CFU/g reduction was achieved by heat treatments at 60℃ for 1 min for DW, SAEW, and SAEW+US, respectively. Regardless of the type of pathogen, the combined treatment (SAEW+US+60℃) achieved a significantly (p < 0.05) longer lag time in all treatment groups. This combined treatment also prolonged the shelf-life of bell pepper up to 8 days and 30 h for the storage at 4℃ and 25℃, respectively. There was also no significant difference in the color and hardness of treated (SAEW+US+60℃) bell pepper from that of control during the storage. This new hurdle approach is thus expected to improve the microbial safety of bell peppers during storage and distribution.

• 한국습지학회지
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• 제26권2호
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• pp.168-181
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• 2024

인공습지는(CW)는 침투, 흡착, 저류, 식물과 미생물의 증발산 등과 같은 수문학적 및 생태학적 기작에 의하여 오염물질 제거, 탄소흡수 및 저장, 생물다양성 향상 등의 생태계서비스를 제공한다. 본 연구는 수평지하흐름 인공습지(HSSF CW)의 미생물 군집과 토양의 물리·화학적 특성 및 처리효율의 상관관계를 분석하기 위하여 수행되었다. 연구를 위한 모니터링은 강우시 수질특성, 토양특성, 미생물 분석이 수행되었다. 따뜻한 계절(>15℃) 에서 TSS, COD, TN, TP 및 중금속(Fe, Zn, Cd) 제거효율이33~74% 범위로 나타났다. 그러나 추운 계절(≤15℃)에서 TOC 35%로 가장 높은 제거 효율이 나타났다. 인공습지 내 토양은 인근에서 채취한 토양의 토양유기탄소(SOC) 함량보다 3.3배 더 높은 함량을 가지고 있는 것으로 나타났다. 유입부와 유출부의 탄소(C), 질소(N) 및 인(P)의 화학양론비(C:N:P)는 각각 120:1.5:1 및 135.2:0.4:1로 나타났으며, 탄소에 비해 질소와 인의 비율이 매우 낮아 미생물 성장에 문제가 발생될 수 있다. 미생물 분석에서는 생물다양성 지수를 통해 미생물 군집의 풍부도, 다양성, 균질성 및 균일성이 따뜻한 계절이 추운 계절에 비해 높게 나타났다. 인공습지의 강우유출수 오염물질 중 질소고정 미생물인 Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes가 우점종으로 미생물 생장을 촉진하는 것으로 평가되었는데 이는 특정 토양특성 및 유입수 특성이 미생물 풍부도와 밀접한 관련이 있음을 의미한다.

• 한국환경보건학회지
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• 제27권4호
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• pp.15-24
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• 2001

The objective of this study it to evaluate the microbial safety in rapid sand filters adapted in most drinking water treatment plants. The potential pathogens to cause water quality problems Are presumed to be Giardia and Gryptosporidium. They look like particles in view of their size. It has been reported that if the number of particles (larger than 2 ${\mu}{\textrm}{m}$ in water) is less than 100 per mL and its turbidity is below 0.1 NTU, it is considered as a safe water in terms of pathogens. In order to achieve such a good water quality. filter-aids (chemicals) were added to the inlet-channel of filter and their effectiveness was evaluated on the basis of water quality factors such as turbidity and particle counting. This study was conducted in she three steps of experiment: jar test. pilot plant test and real water treatment plant test (P plant in seoul). The experiment reult of the P water treatment showed that cationic polyamine was the most effective in the removal of particles and turbidity at the does of 0.25 mg/L. The turbidity without filer-aids showed in the range of 0.12 ~0.17 NTU during filtration and 0.14 NTU on the average. However. with addition of polyamine, the turbidity represented below(or less than) 0.1 NTU after 20 min in the start of filtration and kept 0.08 NTU on the average. On the other hand, as for number of particles, while no filter-aids led to the range of 111 ~270 per mL and 190 on the average, addition of polydmine led to 113 per mL on talc average, and kept below100 per mL after 20 min in the start of filtration.

• Environmental Engineering Research
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• 제19권3호
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• pp.275-281
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• 2014

Aim of this study was to investigate biofilms attached in pipeline of water reuse from the MBR system treating sewage without chlorination in correlation to the outflow water quality. Two general pipe materials: polyvinyl chloride (PVC) and polyethylene (PE) were employed in the experiment. The peak growths were found at week 4 in both pipes. The maximum biofilms in PE pipe was $33mgVSS/cm^2$ with the growth rate of $4.75mgVSS/cm^2-d$ which was significant higher than that of PVC pipe. Biofilms examined by PCR-DGGE technique revealed five bacterial species in PE biofilms namely Sinorhizobium medicae WSM419, Sinorhizobium fredii NGR234, Geobacter sp. M18, Parachlamydia acanthamoebae UV-7, and Mycobacterium chubuense NBB4. The VSS concentrations in outflow had directly correlated to the biofilm attachment and detachment. High COD concentrations of outflow appeared during biofilm detaching phase. In summary, water quality of reuse water corresponded to the biofilms attachment and detachment in the pipeline.

• 한국식품과학회지
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• 제31권5호
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• pp.1196-1202
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• 1999

마이크로파를 이용한 두유의 HTST저온살균시 미생물의 사멸효과와 이화학적 성분 변화를 상법과 비교하고자 하였다. 이때 HTST($90^{\circ}C$, 21초)법으로 살균하되 열수만을 이용한 경우(MP0)와 마이크로파로 온도를 올린 후 열수에서 holding한 경우(MP1)와 마이크로파만을 활용한 경우(MP2)의 세가지 경우로 구분하여 살균전후 변화를 비교해 보았다. 총균수의 경우 세가지 HTST살균법 모두 살균후 초기수의 4 log정도 감소하는 살균효과를 보여주었고 살균효과는 MP0>MP1>MP2의 순서를 보였다. 대장균과 저온성세균 및 phosphatase activity의 경우 세가지 HTST살균법 모두 살균후 우유의 배양실험에서 집락이 확인되지 않거나 살균후 음성(negative)인 것으로 나타났다. 세가지 살균방법 모두 80%이상의 trypsin 잔존율을 보여 trypsin inhibitor가 효과적으로 불활성화되었음을 알 수 있었으며 불활성화 효과는 MP0>MP1>MP2의 순서를 보였다. 두유의 pH와 적정산도, 점도 및 비타민 $B_2$의 경우 살균전후 큰 차이가 관찰되지 않았으며 살균방법별로도 차이가 없었다. 이로부터 마이크로파로 승온시킨 후 holding하는 병용법(MP1)이나 마이크로파 단독처리(MP2)를 활용할 경우 통상적 HTST방법(MP0)보다 우수하거나 대등한 살균효과 및 이화학적 품질보존효과가 있을 것으로 생각되었다. 이러한 연구결과는 다른 액상식품의 살균연구에도 확대 적용할 수 있으리라 기대된다.

• 동아시아식생활학회지
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• 제27권5호
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• pp.576-582
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• 2017

본 연구는 기후변화에 따른 온도 및 습도 상승에 대한 건조분말 식품에서의 미생물적인 품질 변화를 평가하였다. 건조분말 시료를 고온 및 다양한 상대습도에서 저장하며 총균수, 대장균군, 효모 및 곰팡이를 측정한 결과 높은 상대습도에서 건조분말 식품의 수분활성도가 유의적으로 증가하였으며, 총균수와 진균류가 저장기간 동안 유의적으로 증가하는 것으로 나타났다. 특히, 진균류는 저장 기간 동안 건조분말식품의 수분활성도의 증가와 높은 상관성을 나타내어 건조분말 식품의 보관 시 가장 관리가 필요한 미생물 군으로 확인되었다. 반면 상대습도 85% 이하에서는 미생물의 높은 생육이 관찰되지 않아 건조분말 식품의 보관에서 저장 중 상대습도의 관리가 매우 중요함을 알 수 있다. 건조분말 식품은 낮은 수분활성도로 인하여 개봉 후에도 주로 상온에 장기간 보관하는 식품이다. 하지만 본 연구결과로부터 높은 상대습도는 건조분말 식품의 수분활성도와 미생물 품질에 유의적인 영향을 줄 수 있으므로, 개봉된 건조분말 식품은 밀봉하거나, 냉장 보관하는 등 저장 시 온도 및 습도를 철저히 관리할 필요가 있다.

• 한국축산식품학회지
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• 제38권5호
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• pp.936-949
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• 2018

Changes in microbial community and physicochemical traits of chicken summer sausage made from spent layer thigh added with different level (0%, 0.1%, 0.3%, and 0.5% w/w) of ground chopi (Zanthoxylum piperitum) during manufacture were analyzed. The microbial community was profiled and analyzed by sequencing 16S rRNA gene using Illumina MiSeq. Samples were taken from raw sausage batter, after 15 h of fermentation, 8 h of cooking including cooling down, and 7 d of drying. The final pH of the sausage was reduced by the addition of ground chopi. However, no clear effect on water activity was observed. Ground chopi inhibited the development of red curing color after fermentation as it exhibited antimicrobial effect. However, the effect on species richness and microbial composition after cooking was unclear. Ground chopi delayed lipid oxidation during manufacture and the effect was dependent on the addition level. Fermentation reduced the species richness with a dominancy of lactic acid bacteria. The profile of microbiota in the raw batter was different from other stages, while the closest relationship was observed after cooking and drying. Proteobacteria was predominant, followed by Firmicutes and Bacteroidetes in raw samples. Firmicutes became dominating after fermentation and so forth, whereas other predominant phylum decreased. At genus level, unclassified Lactobacillales was the most abundant group found after fermentation and so forth. Therefore, the overall microbial composition aspects were mainly controlled during fermentation by the abundance of lactic acid bacteria, while bacterial counts and lipid oxidation were controlled by cooking and the addition of ground chopi.

• 상하수도학회지
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• 제19권1호
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• pp.47-52
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• 2005

This study was conducted to evaluate the performance of submerged hollow fiber MF processes to treat secondary wastewater for water reuse. Specifically, membrane productivity and filtrate water quality were investigated under various operating conditions (i.e. flux, recovery, and backwash rate) at pilot-scale. Membrane fouling became more severe with increasing flux and recovery, suggesting that low flux operation (< 25 LMH) was desirable. At high flux operating(> 37.5 LMH), increasing backwash rate showed only limited success. The biofouling, quantified by PEPA and BFHPC, was also significant in wastewater reclamation, and biogrowth control by chlorine, were necessary to improve membrane productivity. Filtrate water qualities are in good compliance with water reuse regulations regardless of operating conditions (flux, recovery and backwash rate). Particle (e.g. turbidity) removal ranged from 89 to 98%, while only 11 to 21% of organics (e.g. NPDOC) were removed by MF membrane. Only small improvement in biostability (e.g. AOC) was achieved by MF system, and thus, without post disinfection, significant microorganisms might be present in the filtrate due to regrowth. Lastly, in order to further investigate pathogen removal, controlled microbial challenge tests were performed by monitoring Giardia, Cryptosporidium, bacteria and virus, and showed relatively good microbial removal.