• Title/Summary/Keyword: Microbial stability

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Temperature and microbial changes of corn silage during aerobic exposure

  • Lee, Seong Shin;Lee, Hyuk Jun;Paradhipta, Dimas Hand Vidya;Joo, Young Ho;Kim, Sang Bum;Kim, Dong Hyeon;Kim, Sam Churl
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.7
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    • pp.988-995
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    • 2019
  • Objective: This study was conducted to estimate the temperature and microbial changes of corn silages during aerobic exposure. Methods: Kwangpyeongok (KW) and Pioneer 1543 (PI) corn hybrids were harvested at 29.7% of dry matter and chopped to 3 to 5 cm lengths. Homo (Lactobacillus plantarum; LP) or hetero (Lactobacillus buchneri; LB) fermentative inoculants at $1.2{\times}10^5$ colony forming unit/g of fresh forage was applied to the chopped corn forage which was then ensiled in quadruplicate with a $2{\times}2$ (hybrid${\times}$inoculant) treatment arrangement for 100 days. After the silo was opened, silage was sub-sampled for analysis of chemical compositions, in vitro digestibility, and fermentation indices. The fresh silage was continued to determine aerobic exposure qualities by recorded temperature and microbial changes. Results: The KW silages had higher (p<0.01) in vitro digestibilities of dry matter and neutral detergent fiber than those of PI silages. Silages applied with LB had higher (p<0.001) acetate concentration, but lower (p<0.01) lactate concentration and lactate to acetate ratio than those of LP silages. The interaction effect among hybrid and inoculant was detected in acetate production (p = 0.008), aerobic stability (p = 0.006), and lactic acid bacteria count (p = 0.048). The yeast was lower (p = 0.018) in LB silages than that in LP silages. During the aerobic exposure, PI silages showed higher (p<0.05) temperature and mold than KW silages, while LP silages had higher (p<0.05) lactic acid bacteria and yeast than LB silages. Conclusion: The results indicated that the changes of silage temperature during aerobic exposure seems mainly affected by mold growth, while applied LB only enhanced aerobic stability of PI silages.

The Effect of Microbial Transglutaminase on Textural and Sensory Properties of Noodles Mixed with Rice Flour (미생물유래 Transglutaminase 첨가가 쌀가루 혼합분 반죽과 조리면의 조직감 및 관능특성에 미치는 영향)

  • Shin, Weon-Sun;Seo, Hee-Sun;Woo, Gun-Jo;Jeong, Yong-Seob
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.9
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    • pp.1434-1442
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    • 2005
  • The Present study was attempted to investigate the Possibility of modification of functional properties of the rice flour by crosslinking proteins using microbial transglutaminase (mTGase) derived from a variant of Streptoverticillium sp. MTGase was added at various levels (3,000, 5,000, 7,000 and 10,000 ppm) during making noodles mixed with the rice flour. Mixograph and farinograph showed that imported wheat flour (IWF) had strong dough stability, while the rice flour showed very weak dough strength. However addition of mTGase (3,000, 5,000 and 7,000 ppm) resulted in improvement of dough stability of the rice flour. Texture profile analysis (TPA) results indicated that most of texture parameters (gumminess, chewiness and hardness) of cooked noodles prepared from the rice flour were significantly lower than those of noodles prepared from IWF. However, by addition of mTGase (at the levels of 3,000, 5,000, 7,000 ppm) dough stability and all the TPA values and sensory score (at the level of 7,000 ppm mTGase) on chewiness and hardness of cooked noodles made with $30\%$ rice flour were improved significantly. These results suggest that dough stability and texture of rice noodles as well as sensory characteristics could be improved by addition of mTGase to the rice flour.

Effect of Modified Atmosphere Packaging on Preservation of the Steamed Bread Added with Black Rice (변형기체포장이 흑미 첨가 기능성 찐빵의 저장성에 미치는 영향)

  • Choi, Dong-Man;Kim, Nam Yong;Chung, Sun-Kyung;Kwon, Ho Ryoung;Lee, Dong Sun
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.18 no.1_2
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    • pp.33-38
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    • 2012
  • Modified atmosphere packaging (MAP) was applied to steamed bread added with black rice in 10%. In terms of storage stability, the steamed bread was found to be susceptible to microbial spoilage and could be helped in its preservation and shelf life extension by MAP. According to microbial and sensory quality criteria, the product shelf life was determined as < 5 days for air package, 5 days for 100% $N_2$ package, 8 days for package of 60% $CO_2$/40% $N_2$, and 15 days for 100% $CO_2$ package. Even though MAP condition with $CO_2$ inclusion was effective in extending the shelf life based on microbial quality, it did not affect the staling of the bread.

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The Relationship between Microbial Characteristics and Glomalin Concentrations in Paddy Soils of Gyeongnam Province (경남지역 논토양 미생물 특성과 글로말린 함량 상관관계)

  • Lee, Young-Han;Kim, Min-Keun;Ok, Yong Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.5
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    • pp.792-797
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    • 2012
  • Glomalin-related soil protein has been suggested as an enhancer for soil stability by promoting the aggregation. In this study, we examined the concentrations of glomalin and characteristics of microbial community in 20 paddy soils sampled from Gyeongnam Province. Total soil glomalin as glomalin-related soil protein (GRSP) had a significant positive correlation with soil organic matter (p<0.01) and soil dehydrogenase activity (p<0.01). The concentration of GRSP significantly correlated to soil microbial biomass carbon (p<0.001) and the total bacterial community (p<0.01) in paddy soils. In addition, the GRSP had a significant positive correlation with gram-negative bacteria community (p<0.05) and ratio of cy19:0 to 18:$1{\omega}7c$ (p<0.05) in paddy soils. In conclusion, the concentration of GRSP could be an indicator of soil health that simplify the inspection steps for sustainable agriculture in paddy soils.

Antimicrobial & Physiological Characteristics of Ethanol Extract from Pinus rigida Miller Leaves (리기다소나무 잎 에탄올 추출물의 항균 및 생리특성)

  • Oh, Byung-Tae;Choi, Sung-Gil;Cho, Sung-Hwan
    • Food Science and Preservation
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    • v.13 no.5
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    • pp.629-633
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    • 2006
  • Pinus rigida Miller leaf extract (PRLE) showed antimicrobial activity remarkably against food pathogenic and spoilage bacteria at concentrations of $100{\sim}250{\mu}g/mL$. Alcohol-soluble PRLE had higher antimicrobial activity against Staphylococcus aureus and E-coli than any other-soluble PRLE such as butanol, ethyl acetate, ether and water. As PRLE concentration increased alcohol-soluble PRLE increased the remarkable inhibitory zone of microbial growth on the microbial media. PRLE showed good stability against temperature and pH in the range of $40{\sim}150^{\circ}C$ and $4{\sim}11$, respectively. This may indicate that PRLE can be a potential anti-microbial agent for industrial application. In addition, SEM of Listeria monocytogenes suggested that it antimicrobial component would perturb the functions of microbial cell membranes synergistically. In the feeding experiment the formaldehyde content in the serum of formalin-fed and PRLE-treated me decreased remarkably due to the lysis of formaldehyde and the rate of hemoglobin biosynthesis was recovered to the orignal state within a short breeding time.

Immobilization of Microbial Cells and Or-ganelles by Entrapment with Urethane Prepolymers

  • Jin, Ing-Nyol
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1979.04a
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    • pp.115.4-116
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    • 1979
  • Acetone-dried cells of Arthrobacter simplex were entrapped in several preparations of hydrophilic urethane prepolymers and their steroid converting ability was examined. SeVeral solvents, such as methanol and propylone glycol, wereeffective for the conversion of hydrocortisone to prednisolone. The stability of the immobilized cells during storage and on repeated reactions was also examined. Thisconvenient entrapping method was also applicable for the immobilization of cellular organelles. yeast peroxisomes. The entrapped peroxi-somessh owed the activities of alcohol oxidase and catalase.

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Structural Changes of Poly(tetrametylene succinate)/Polycarbonate copolymers on Hydrolysis (Poly(tetrametylene succinate)/Polycarbonate copolymer의 가수분해시의 구조변화)

  • Wanduk Lee;Min Shin;Seungsoon Im
    • Proceedings of the Korean Fiber Society Conference
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    • 2003.10b
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    • pp.209-210
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    • 2003
  • The durability and anti-microbial stability of plastics, which have been thought to be favorable characteristics, cause ecoloical problems due to non-degradation after disposal. For a possible solution of these ecological and environmental problems, the biodegradable polymers. especially aliphatic polyesters, have been widely investigated. Poly(tetramethylene succinate)(PTMS) is one of the most promising biodegradable polyesters. (omitted)

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김치 발효소시지가 미생물학적 안정성에 미치는 효과

  • Lee, Ju-Yeon
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2004.05a
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    • pp.61-87
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    • 2004
  • ${\cdot}$ The LAB as an integrated part of kimchi were well adapted to the new habitat of fermenting sausage and exhibited good souring properties that are comparable to those commercial starter cultures. ${\cdot}$ With the added kimchi (5-15%) and kimchi-powder (2-5%), the necessary microbial stability of real fermented sausages was achieved. ${\cdot}$ In particular, kimchi-powder contributed to improving the safety of the fermented sausages as compared to the conventional one treated with starter culture.

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A Study on the Improvement in Impact Resistance and Thermal Stability of Microbial Polyester by Biodegradable Plasticizer (생분해성 가소제에 의한 미생물 폴리에스테르의 내충격성 및 열안정성 증진에 대한 연구)

  • 최재신;김소현;이택승;박원호
    • Proceedings of the Korean Fiber Society Conference
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    • 2001.10a
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    • pp.199-202
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    • 2001
  • 현재 플라스틱에 의한 환경오염이 사회문제로까지 확대되면서 이를 해결하기 위한 한가지 대책으로 분해성 플라스틱의 개발이 절실히 요구되어 전세계적으로 많은 연구가 이루어지고 있다. 생분해성을 가지는 미생물 폴리에스테르 중에서 가장 대표적인 poly(3-hydroxybutyrate), PHB는 우수한 역학적 성질을 가지며, 또한 천연고분자 중에서 융점을 가지는 유일한 열가소성 재료이다. (중략)

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