• Asian-Australasian Journal of Animal Sciences
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• 제13권4호
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• pp.490-496
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• 2000

In vivo digestibility, nitrogen retention and microbial protein yield from diets of 100% ammonia treated rice straw (ARS) ($D_1$); 65% untreated rice straw (URS)+30% rice bran (RB)+5% SBM ($D_2$) and 85% ARS+15% RB ($D_3$) were determined using three Japanese Corriedale wethers in a $3{\times}3$ Latin Square Design. Results showed that DM consumption and organic matter digestibility were highest in $D_3$; but did not promote high protein digestibility, which RB+SBM had effected in URS based-diet. Dry matter intake and OM digestibility were the same for $D_1$ and $D_3$. Solubility of fiber bonds was increased by ammoniation, resulting in higher NDF digestibility. Nitrogen retention and microbial protein yield of rice bran supplemented groups was higher than ARS, but supplementation did not significantly increase efficiency of microbial protein synthesis from ARS which did occur when RB+SBM was added to untreated straw. The quality of ammoniated rice straw could be improved through RB supplementation because of its positive effects on DM digestibility, nitrogen retention and microbial protein yield. However, the addition of RB+SBM to URS resulted to more efficient N utilization.

• 한국토양비료학회지
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• 제34권1호
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• pp.8-16
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• 2001

미생물제 비료시용이 배추의 생육과 토양의 화학성 변화 및 미생물상에 미치는 영향을 포장시험으로 실시하였다. 대조구에 비하여 TLB미생물제 비료를 시용함으로써 배추의 생육은 양호하였으며, 배추수량에 있어서도 유의성 있는 증수 효과를 보였다. 그러나 TLB미생물제 비료를 시용하더라도 배추생육시 요소비료의 추비량 및 퇴비 시용량을 줄일 경우에는 대조구에 비하여 수량이 다소 감소하는 경향을 보였다. 토양의 화학적 성질에 미치는 TLB 미생물제 비료 시용의 효과는 대조구에 비하여 수확기의 토양유기물 함량의 감소가 가장 큰 것으로 나타났고, 토양중 전질소 함량은 0.76~1.44% 범위로 대조구보다 오히려 미생물제 비료 시용구가 감소하는 경향을 보였다. 토양중 유효인산 함량은 시험전 토양의 559ppm에 비하여 배추의 수확기에 대조구 755ppm 그리고 미생물제 시용구 653ppm으로 대조구와 비교하여 미생물제 시용으로 인하여 다소 낮은 편이었다. 한편 배추 수확 후 배추의 화학적 성분은 전 질소 2.62~2.94%, 인산 1.48~1.55%, 칼리 3.60~4.38%범위이었으며, 각 처리간에 함량의 뚜렷한 차이는 없었다. 토양 미생물상중 전세균수는 대조구보다 미생물제 시용으로 다소 감소하였으나, 배추수확기 토양에서 Pseudomonas속이 3배 이상 높은 군락을 보였다. 그러나 방선균은 큰 차이를 보이지 않았지만, 사상균수는 수확기 토양에서 미생물제 비료 시용으로 현저히 높은 군락을 형성하였다.

• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.69-74
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• 2000

The medium for erythritol production by Torula sp. in a 500-ml baffled flask was optimized to be 300 g/I sucrose, 10 g/I yeast extract, 3 g/I $KH_2PO_4$, and 10 mg/I $CuSO_4{\cdot}5H_2O{\;}at{\;}34^{\circ}C$ with initial pH of 5.5. Using this optimal medium, erythritol of 166 g/I was obtained after 140 h of cultivation, corresponding to 55.3% of the erythritol yield from sucrose with a productivity of 1.11 g/I/h. Optimal concentrations of carbbon and nitrogen sources in a fermentor were higher than that in a flask due to the higher oxygen supply of the fermentor. Employing the medium containing 300 g/I or 400 g/I sucrose for the determination of optimal C/N ratio, the C/N ratio was found to be more important than the nitrogen concentration for effective erythritol production, The optimal ratio of yeast extract to sucrose (g/g) was 20. The yield and productivity of erythritol were maximal in the medium containing 400 g/I sucrose and 20 g/I yeast extract. when dissolved oxygen in the culture was increased, the cell mass increased but the erythritol production was manimal in the range of 5 to 10% of dissolved oxygen. Under the optimal the rane of 5 to 10% of dissolved oxygen. Under the optimal culture condition of the fermentor, a final erythritol concentration of 200 gI was obtained after 120 h with a yield of 50% and the productivity was 1.67 g/I/h. The yield was the highest among erythritol-producting microorganisms

• 한국환경농학회지
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• 제17권3호
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• pp.254-259
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• 1998

농가에서 실행되고 있는 유기농업의 실증적 연구를 위하여 돈분, 또는 돈분과 계분을 혼합하여 만든 발효퇴비를 10a 당 3, 6, 12ton씩 시비하고 다시 미생물제를 처리한 후 토마토의 생육, 수량 및 토양 중 양분의 변화를 조사하였던 바 다음과 같은 결과를 얻었다. 1. 토마토 초장은 퇴비 시용구에서 관행구에 비하여 길었으며 엽폭은 관행구에서 더 긴 것으로 나타났다. 엽록소 함량은 관행구에서 계속적으로 높은 값을 보였으며 퇴비 시용량의 차이에 따른 엽록소 함량의 차이는 정식 45일 후부터 아주 낮아지는 것으로 나타났다. 2. 미생물제를 사용하여 충분히 발효시킨 경우 12ton/10a의 시용에서 토마토의 수량이 관행구의 50-60%에 불과하였으나, 재배도중 추비하였을 경우에는 6ton/10a 시용구에서 관행구의 82%정도에 달하는 수량을 얻었다. 3. 일차 발효된 퇴비의 시용 후에 미생물제의 토양 살포는 질소성분의 빠른 유실을 초래하였으며 토양중 미생물의 밀도를 상당히 높혀주는 것으로 나타났다. 4. 과실의 색깔은 모든 퇴비 시용구에서 관행구에 비하여 붉은 색이 선명하게 나타났으며, 당도와 산도는 퇴비 시용량이 많은 12ton/10a 처리구에서 가장 높게 나타났으며, 경도는 관행구에서 유기물 시용구에 비하여 높게 나타났다. 5. 퇴비 시용구에서 $NO_3-N$와 $NH_4-N$의 함량은 시용 직후에는 많은 양이 검출되었으나 급속히 감속되어 4-5주 후부터 아주 미량이 검출되었다. 그러나 인산, 칼륨, 마그네슘 및 칼슘의 함량은 서서히 감소되는 경향을 보였다.

• 한국환경농학회지
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• 제19권5호
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• pp.375-381
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• 2000

Quantifying the changes of soil microbial biomass and activity of enzymes are important to understand the dynamics of active soil C and N pools. The dynamics of soil microbial biomass C and N and the activity of enzymes over entire growth period of soybean-(Glycine max (L) Merr.)-wheat (Triticum aestivum L.) sequence on a Typic Haplustert as influenced by organic manure and inorganic fertilizer N were investigated in a field experiment. The application of farmyard manure at 4 to 16 $Mg{\cdot}ha^{-1}\;y^{-1}r^{-1}$ along with fertilizer nitrogen at 50 or 180 $kg{\cdot}ha^{-1}$ increased the mean soil microbial biomass from 1.12 to 2.05 fold over unmanured soils under soybean-wheat system. Irrespective of organic and chemical fertilizer N application, the soil microbial biomass was maximum during the first two months at active growing stage of the crops and subsequently declined with crop maturity. The mean annual microbial activity was significantly increased when manure and chemical fertilizer at 8 $Mg{\cdot}ha^{-1}$ and 50/180 N $kg{\cdot}ha^{-1}$, respectively were applied. The C turnover rate decreased by 47 to 72 % when the level of farmyard manure was increased from 4 to 8 and 16 $Mg{\cdot}ha^{-1}$. There were significant correlations between biomass C, available N, dehydrogenase, phosphatase and yield of the crops.

• 환경생물
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• 제23권4호
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• pp.383-389
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• 2005

The effect of different mulching materials on mungbean production was studied. The general objective was to assess the ecological effects of mulching materials in sustainable mungbean production. Specifically, the study aimed to determine the effects of different mulching materials on the chemical, physical and biological soil properties, on weed control and yield, and to identify mulching materials that are environmentally friendly in mungbean production. The experiment was conducted at the Fruit and Vegetables Seeds Center, Science City of $Mu/tilde{n}oz$, Nueva Ecija, Philippines from May to July 2004. The initial soil chemical properties were: pH of 6.4, 2.0 percent organic matter content, 0.10 percent total nitrogen, 22 ppm phosphorus, and 370 ppm available potassium. The soil microbial loads were $8\times10^4\;CFU\;g^{-1}$ for bacteria and $14\times10^4\;CFU\;g^{-1}$ for fungi. Mushroom spent mulch increased soil organic matter with an average of 3.13 percent, nitrogen with an average of 0.16 percent and the highest number of bacterial count with $3.4\times10^8\;CFU\;g^{-1}$. Use of mulch, except rice straw mulch, generally increased mungbean yield. The best mulching material for high yield production of mungbean was black polyethylene plastic film, although environmentally unfriendly.

• 한국토양비료학회지
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• 제49권5호
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• pp.531-540
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• 2016

The adoption of legume cover crops in no-tillage system can contribute to improve soil fertility by providing several benefits, including reduction in soil erosion, suppression of weed growth and N supply to subsequent crops. We conducted a field study to investigate the effect of cover crops and nitrogen fertilization rates on yield and nitrogen use efficiency of waxy corn (Zea mays L.) in no-tillage upland field. Two legume cover crops, hairy vetch (Vicia villosa Roth) and crimson clover (Trifolium incarnuturn L.) were mechanically terminated with roller in early June. For each cover crop treatment, nitrogen (N) fertilizer was applied at three different rates (145, 72.5 and $0kg\;N\;ha^{-1}$). The growth and yield characteristics of corn were significantly affected by the N fertilization rates in crimson clover plots, which suggest N mineralization from the cover crop residue was not sufficient. In contrast, N fertilization rates had no significant effect on growth and yield of corn in hairy vetch plots, indicating that the amount of N released from the cover crop is large enough to meet most of the N requirement of corn. However, the application of N fertilizer in hairy vetch cover plots resulted in slight increase of crop yield, though not statically significant, and high levels of N concentration in corn plant tissue possibly due to luxury consumption of N. Organic residues on the soil surface in hairy vetch cover plots had substantial amounts of N after harvest, ranging from 100 to $116kg\;N\;ha^{-1}$, which is presumably retained during winter season and released by microbial mineralization in subsequent year. The highest nitrogen yield efficiency was achieved in the plot with hairy vetch cover and no N fertilizer application, followed by the plot with hairy vetch cover and $72.5kg\;N\;ha^{-1}$ fertilization rate. In conclusion, hairy vetch showed better performance in corn productivity as compared with crimson clover. In addition, it was concluded that the application of N fertilizer between 0 and $72.5kg\;N\;ha^{-1}$ in combination with hairy vetch cover crop might be most efficient for corn yield under no-tillage system with climatic and soil characteristics similar to those of the experimental site.

• 상하수도학회지
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• 제8권1호
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• pp.18-26
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• 1994

This research investigated efficient operation mode for the successful performance of SBR(sequencing batch reactor) treating fish processing wastewater, and the effect of sodium chloride (NaCl) on treatment efficiency. 2-hour-annerobic, 6-hour-aerobic and 3-hour-anoxic operation during reaction period was found an effective operating method for organic and nitrogen removal from fish processing wastewater in SBR system. The average removal efficiencies of COD, BOD, and total nitrogen in SBR operated continuousely were 91%, 95%, and 67.1%, respectively. The estimated values of biomass yield coefficient(Y), microbial decay coefficient($K_d$), and bioreaction rate constant(K) were $0.35gMLSS/gCOD_{removed}$, $0.015day^{-1}$, and $0.209hr^{-1}$, respectively. As NaCl concentration increased from 5 to 30g/L, sludge settleability was cnhanced but organic removal in the reactor was decreased. NaCl of influent had considerable relationship with COD removal, whereas it did not significant affect nitrogen removal.

• Asian-Australasian Journal of Animal Sciences
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• 제10권5호
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• pp.460-470
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• 1997

On an absolute straw diet, the effect of graded levels of green grass supplementation on intake, nutrient digestibility, rumen fermentation pattern and microbial N yield has been studied in cattle. Of the two trials conducted, 16 intact growing bulls of 304 kg weight and 32 months old, randomly allocated to four treatments in a completely randomized design in the 1st trial. While, in the, 2nd trial, four rumen cannulated local bulls of about 60 months old and 400 kg weight were used in a $4{\times}4$ Latin square design with four treatments in four periods. In both the trials, in addition to a mineral mixture, animals were supplemented with graded levels of naturally grown green grass of 0 kg (T1), 2 kg (T2), 4 kg (T3) or 6 kg (T4) to an ad libitum rice straw diet In the 1st trial, measurements were made on intake digestibility, growth rate, N balance and microbial N yield. While in the 2nd trial, in addition to the above parameters (except growth rate), rumen parameters were also studied. All levels of grass supplementation decreased the straw DM intake and increased the substitution rate. The rumen $NH_3-N$ concentration increased with the increase in grass level and ranges from 8-46 mg/l. The rumen pH and the rate and extent of DM degradability of straw were not affeceted by different rumen environments created by different levels of grass inclusion. At 48 h, straw DM degradability were 42, 44, 44 and 43% respectively for 0, 2, 4 and 6 kg grass supplementation daily. The whole gut digestibilities of DM, OM and ADF increased significantly (p < 0.05) only at 6 kg level daily. The microbial N yield was not affected by the levels of grass supplemented. The mean microbial N yield was 10 (SD 3.7) g/kg DOM apparently fermented in the rumen. The estimated minimum N loss and thus the maintenance requirement of tissue protein was 303 mg/kg $W^{0.75}/d$. All the animals lost live weight but 6 kg grass supplementation gave positive energy and N balances. Small amount of green grass supplementation is often recommended for optimization of rumen environment of a straw diet However, under the present experimental condition, no such beneficiary effect observed up to 6 kg (26% of DM intake) level of supplementation.

• 한국미생물·생명공학회지
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• 제40권1호
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• pp.30-38
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• 2012

두 종의 Bacillus와 한 종의 Pseudomonas로 구성된 컨소시엄 미생물제제를 고추 경작지에 첨가하였을 때 경작지 토양의 미생물 다양성 변화를 조사하기 위해 건전한 토양의 지표가 되는 질소순환관여 미생물과 근권토양 내 섬유소 분해기능, 인산가용화기능과 urea 가수분해기능을 가지는 기능성미생물의 활성과 밀도를 측정하였다. 고추 경작지 토양에 컨소시엄 PGPR 미생물인 B. subtilis AH18, B. licheniformis K11과 P. fluorescens 2112의 컨소시엄 미생물제제를 처리하여 토양미생물의 다양성에 변화를 측정하여 컨소시엄 미생물제제의 처리에 의한 근권토양 미생물의 다양성 변화와 고추의 생육에 미치는 영향을 분석하였다. 컨소시엄 미생물제제 처리구의 경우 Actinomyces spp., Trichoderma spp., 광합성세균과 Azotobacter spp.가 다른 처리구에 비하여 1.7-5배 이상 높았으며, 질소 순환에 관여하는 미생물은 1.4-4배 증가되었다. 또한 기능성 미생물의 다양성도 화학제제나 물처리구와 비교하였을 때 1.3-3배 증가하였다. 건고추 수확량은 컨소시엄 미생물제제를 처리하였을 경우 대조구나 화학제제를 처리하였을 때보다 15%이상 수확량이 증대되었다. 따라서 PGPR 컨소시엄 미생물제제의 처리로 인해 고추 경작지 토양의 근권미생물 다양성과 작물의 생산성에 긍정적인 영향을 주는 것으로 생각된다. 추후 메타지노믹스를 활용하여 미생물 다양성 변화를 추가적으로 확인할 예정이다.