• Title/Summary/Keyword: Microaggregation

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Study on the Inoculation Augmentation of paecizomyces japonicus to the Silkworm, Bombyx mori, Using Dexamethasone (Dexamethasone을 이용한 누에(Bombyx mori)에 대한 동충하초균 (Paecilomyces japonicus)의 접종율 제고에 관한 연구)

  • 김길호;박영진;김용균;이영인
    • Korean journal of applied entomology
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    • v.40 no.1
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    • pp.51-58
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    • 2001
  • Entomopathogenic fungus, Paecilomyces japonicus, has been commercially used as medicinal purpose . The silkworm, Bombyx mori, as an optimal host for the fungi, has been selected and used for the production of the fungal fruit bodies. In current method, newly molted fifth instal larvae should be exposed to the adverse stress environment of high temperature (3$0^{\circ}C$), high relative humidity ( 90%), and starvation for 24h for better fungal inoculation to the host insects. In this study, an alternative method using chemical agent, dexamethasone (DEX: an eicosanoid biosynthesis inhibitor), was tried to get the immunodepressive effect on the larvae to elevate the inoculation rate of the fungi to the silkworm without any harsh rearing environment. DEX (100$\mu\textrm{g}$) showed significantly synergistic effect on the hemocyte lethality of the fungus, and was effective to decrease cellular immune responses measured by the number of hemocyte microaggregation and phenoloxidase activity of the fifth instar larvae in response to the fungal injection. A detergent of 0.05% Triton-X was effective to increase the in- oculation rate of the fungi to the larvae and used in all fungal spraying solutions. Without any environ- mental stress treatment, only DEX (100$\mu\textrm{g}$) injection to the fifth instar larvae followed by the fungal spray was effective to get the inoculation rate equivalent to the current fungal spray method requiring harsh rearing environment. These results suggest that the inoculation of P. japonicus can be elevated by the help of DEX and that the silkworms use eicosanoids to elicit cellular immune response against fungal pathogen.

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Statistical disclosure control for public microdata: present and future (마이크로데이터 공표를 위한 통계적 노출제어 방법론 고찰)

  • Park, Min-Jeong;Kim, Hang J.
    • The Korean Journal of Applied Statistics
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    • v.29 no.6
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    • pp.1041-1059
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    • 2016
  • The increasing demand from researchers and policy makers for microdata has also increased related privacy and security concerns. During the past two decades, a large volume of literature on statistical disclosure control (SDC) has been published in international journals. This review paper introduces relatively recent SDC approaches to the communities of Korean statisticians and statistical agencies. In addition to the traditional masking techniques (such as microaggregation and noise addition), we introduce an online analytic system, differential privacy, and synthetic data. For each approach, the application example (with pros and cons, as well as methodology) is highlighted, so that the paper can assist statical agencies that seek a practical SDC approach.