• Genomics & Informatics
• /
• v.3 no.1
• /
• pp.15-23
• /
• 2005

Background MicroRNAs (miRNAs) are a class of noncoding RNAs found in various organisms such as plants and mammals. However, most of the mRNAs regulated by miRNAs are unknown. Furthermore, miRNA targets in genomes cannot be identified by standard sequence comparison since their complementarity to the target sequence is imperfect in general. In this paper, we propose a kernel-based method for the efficient prediction of miRNA targets. To help in distinguishing the false positives from potentially valid targets, we elucidate the features common in experimentally confirmed targets. Results The performance of our prediction method was evaluated by five-fold cross-validation. Our method showed 0.64 and 0.98 in sensitivity and in specificity, respectively. Also, the proposed method reduced the number of false positives by half compared with TargetScan. We investigated the effect of feature sets on the classification of miRNA targets. Finally, we predicted miRNA targets for several miRNAs in the Caenorhabditis elegans (C. elegans) 3' untranslated region (3' UTR) database. Condusions The targets predicted by the suggested method will help in validating more miRNA targets and ultimately in revealing the role of small RNAs in the regulation of genomes. Our algorithm for miRNA target site detection will be able to be improved by additional experimental­knowledge. Also, the increase of the number of confirmed targets is expected to reveal general structural features that can be used to improve their detection.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.21 no.8
• /
• pp.760-765
• /
• 2008

Low power laser therapy is internationally certified and is known to be effective in stimulating DNA in living organisms, increasing protein synthesis and activating cell division, smoothing blood circulation, promoting cell activation, cell regeneration and function. It also has anti-inflammatory, anti-edemic, anti-fibrous dysplastic and neuralogic hyperfunctional effects. This study was intended to verify the effect of LED irradiation therapy on wound healing in cell and animal tests by applying LED irradiator using a laser and laser diode, which was independently designed and developed to emit beams of similar wavelength to that of a laser. This equipment was fabricated using a micro-controller and a high brightness LED, and designed to enable us to control light irradiation time, intensity and reservation. In case of cell proliferation experiment, each experiment was performed to irradiation group and non-irradiation group for tissue cells. MTT assay method was chosen to verify the cell increase of two groups and the effect of irradiation on cell proliferation was examined by measuring 590 nm transmittance of micro-plate reader. In the wound healing experiment, 1$cm^2$ wounds on the skin wound of SD-Rat(Sprague-Dawley Rat) were made. Light irradiation group and none light irradiation group divided, each group was irradiated one hour a day for 9 days. As a result, the cell increase of tissue cells was verified in irradiation group as compared to non-irradiation group. And, compared with none light irradiation group, the lower incidence of inflammation and faster recovery was shown in light irradiation group.

• Ocean and Polar Research
• /
• v.44 no.4
• /
• pp.331-338
• /
• 2022

The high latitude scleractinian coral Alveopora japonica Eguchi, 1965 occurs in high density in the shallow rocky subtidal in Jeju Island, forming coral carpets. Despite its ecological role providing a unique habitat for other benthic organisms, the benthic fauna associated with the A. japonica coral carpet is poorly known. To identify fauna associated with the coral carpet, we explored three sites dominated by A. japonica and one control site on northwestern Jeju Island in May 2013. Using SCUBA, we collected A. japonica and the epibenthic mega-fauna associated with the colonies in 1×1 m² and identified them to the species level. At a depth of 10 to 15 m, A. japonica colonies heavily covered the seafloor, forming a layer called a coral carpet, with a density of 94 (Keumneung-ri), 133 (Biyangdo), and 155 (Gwidok-ri) colonies/m². Thirty-four molluscan species were identified from the four sites, including 20 bivalves and 14 gastropods. The coral carpets were enriched with sessile bivalves compared to the control site, as we identified twenty bivalve and eight gastropod species from the coral carpets. Most bivalve species associated with the coral carpets had tropical-subtropical affinities, while gastropods were mainly subtropical and subtropical-low boreal species. Leiosolenus lischkei M. Huber, 2010, in the family Mytilidae and Barbatia steamsi (Pilsbry, 1895), in the family Arcidae, were the two most abundant bivalve species in the coral carpet, L. lischkei being a borer, and B. stearnsi a nestler. The tropical to subtropical Pacific star shell Astralium haematragum (Menke, 1829)was the most abundant gastropod at the study sites. The bivalves and gastropods associated with the coral carpet were small-sized juveniles or sub-adults, suggesting that the coral carpet provides a micro-habitat for the bivalves and gastropods.

• Korean Journal of Medicinal Crop Science
• /
• v.14 no.6
• /
• pp.336-341
• /
• 2006

The endophytic bacteria were isolated from the rusty-root ginseng. This isolated bacteria were occurred the rusty-root ginseng with artificial inoculation. For the suppressing of rusty-ginseng, disinfectants, antibiotics, kitosan, micro-organisms and metabolites were tested to isolated endophytic bacterium. All of the isolated bacteria strains were sensitive sodium hypochlorite, however, some of isolated bacteria lines were sensitive to other tested materials. For example, D (didecyl dimethyl ammonium bromide), CIO$_2$, ODDA (octyldecyl dimethyl ammonium chloride + diocyul dimethyl ammonium chloride + alkyl diethyl benzyl ammonium chloride), GD (glutaraldehyde + dimethy cocobenzyl ammonium chloride) suppressed some of bacteria strains. Otherwise, some of antibiotics (e.g. ampicillin, chloramphenicol, erythromycin, kanamycin, neomycin, rifampin, streptomycin, tetracycline) were sensitive to the isolated bacteria strains. All of isolated bacteria strainswere inhibitive to the mixed formation with neomycin and streptomycin, and neomycin and tetracycline. Both sodium hypochlorite and antibiotic mixing of neomycin and tetracycline were effective to prevention of rusty-root ginseng of sub-merging ginseng in the ginseng field.

• Korean Journal of Soil Science and Fertilizer
• /
• v.45 no.6
• /
• pp.936-942
• /
• 2012

This review was to elucidate the fate of Bentazon(3-isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one-2,2-dioxide) and its metabolites in soil. Bentazon is rapidly degraded to form polar metabolites which are mostly adsorbed to soil components, such as humin or fulvic acid, as non extractable forms and mineralized into $CO_2$ by light or micro-organisms in both aerobic or nonaerobic condition. The degradation of Bentazon is dependent on the rate of organic matters in soil and the use of land for the tillage. The degradation rate is decreased as the amount of organic matters in soil increases and if the land is under use for tillage. Sorption and mobility of Bentazon depends on soil pH and the content of organic matters in soil. Usually, the sorption of the metabolites of Bentazon is decreased with increase in the mobility and pH. Almost all of Bentazon is degraded within rhizosphere or forms conjugate bonds with soil organic matters before it reaches to the ground water.

• Fisheries and Aquatic Sciences
• /
• v.2 no.2
• /
• pp.172-175
• /
• 1999

Relative DNA contents in some harmful algae were measured using DAPI staining and image analysis system. This method was useful to identify some morphologically similar species and isolates from harmful algal blooms (HABs). In exponential phase, Prorocentrum micans had higher relative DNA content (RD) of $1.83\pm0.52$ than any other isolates, followed by Cochlodinium polykrikoides $(1.10\pm0.46)$ Alexandrium tamarense $(0.93\pm0.32)$ Gyrodinium impudicum $(0.56\pm0.17)$, Scrippsiella trochoidea $(0.41\pm0.26)$ and P. minimum$(0.05\pm0.01)$. When they were fixed with Lugol's solution, it was difficult to d,iscern C. polykrikoides from G. impudicum under the light microscope, but the DNA contents were quite different in two species. C. polykrikoides contained about twice as much RD as G. impudicum under the same culture conditions and exponential phase. DAPI­stained DNA feature in C. polykrikodes showed concentrated in the peripheral part of the cell, but in G. impudicum showed a compact structure in the central part. Although A. tamarense and S. trochoidea were morphologically similar under the light microscope, nuclear DNA content of A. tamarense was twice as much as that of S. trochoidea.

• Journal of Radiopharmaceuticals and Molecular Probes
• /
• v.4 no.1
• /
• pp.36-42
• /
• 2018

Molecular imaging refers to detect the biochemical process in living organisms at the cellular and molecular levels and to quantify them. Due to several advantages of nanomaterials, various molecular images using nanomaterials are being tried. Attempts have been made to combine nanoparticles, known as micro- or nanosized nanomaterials, with radioactive isotopes for molecular imaging probe. The radiolabeled nanoparticles will expend the molecular imaging due to nanoparticle's size-dependent nature. In particular, iron oxide nanoparticles can be used for magnetic resonance imaging, can be adjusted in size, easily functionalized, and biocompatible, making it a very good platform for molecular imaging. In addition, iron oxide nanoparticles may be the best example for a new approach to molecular imaging techniques. In this paper, we introduce various methods for preparation of iron oxide nanoparticle combined with radioisotope starting from various synthesis methods of iron oxide nanoparticles to utilize iron oxide nanoparticles as a platform for molecular imaging through radioactive labeling.

• Journal of Environmental Health Sciences
• /
• v.24 no.1
• /
• pp.104-111
• /
• 1998

The utilization of food garbage as composting was investigated by using the batch reactor and by varying the initial temperature of the fermentation reactors. As the straw controlled under 50 to 55% of moisture content and mixed 5% of EM(Effective Micro-organisms) microbial agent. An agitator continuously operated 1 rpm, supplying the amount of air(2l/kg.min). Reactor temperature changed three type of 40$\circ$C, 50$\circ$C, 60$\circ$C. In the case of 50$\circ$C operated 72 hr after organic contents showed lowest 48%, and weight reduction rate of showed 77%. The reaction gas was showed 30 min after 19. 9% of the lowest level at 20.9% concentration of oxygen and CO$_2$ gas was produced 0.9% due to organic disintegration on initial react time. NH$_4$, H$_2$ and CH$_4$ gas concentration showed 589 ppm lhr after, 83 ppm and 0.3%, but 8hr after gas product was complete. As using the straw of bulking agent, the Reduction by disintegration should be more effectively than composting.

• YAKHAK HOEJI
• /
• v.21 no.4
• /
• pp.193-199
• /
• 1977

ABS, Alkyl benzene sulfonate, persists for long periods in stream because of its resistance to biologic degradation. Its bio-degradation is very varied in the environments. This investigation was therefore undertaken in order to know the biodegradation of synthetic detergents which are comprising soft and hard forms from February 1976 to September 1976. The biodegradations by spontaneuos settling and aeration were determined. The reduction rates of ABS, DBS, and LAS were 9.8%, 13.7% and 10.4% by the settlings for 5 days at $25^{\circ}$ and 63.3%, 27.2%, and 43.9% by aeration respectively. It was not contributed effectively to biodegradate the synthetic detergents by the simulaneous incubation with micro-organisms isolated from sewage such as Enterobacter sp., E. coli, Bacillus sp., Flavobacterium sp., Pseudomonas sp., Staphylococcus sp., and etc. Tricking filter could also eliminate some amount of detergent. By the application of this investigation, it could be designed that detergents would be regulated in abuse in context with water pollution and be treated at a proper process in the sewage treatment plant to be installed.

• Particle and aerosol research
• /
• v.5 no.4
• /
• pp.171-178
• /
• 2009

$PM_{2.5}$ is one of critical air pollutants due to its high absorbability of heavy metallic fumes, PAH and bacillary micro organisms. Such a fine particulate matter is often formed through various nucleation processes including condensation. This study attempts to find the nucleation behaviors of $PM_{2.5}$ arisen from coal power stations using a classical heterogeneous Fletcher's theory. The numerical simulation by C-language could approximate the nucleation process of $PM_{2.5}$ from water vapor, of which approach revealed the required energy for embryo formation and embryo size and nucleation rate. As a result of the calculation, it was found that wetting agents could affect the particle nucleation in vapor condensation. In particular, critical contact angle relates closely with the vapor saturation. Particle condensation could be reduced by lowering the angles. The wetting agents aid to decrease the contact angle and surface tensions, thereby may contribute to save the formation energy.