• Asian Pacific Journal of Cancer Prevention
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• 제16권4호
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• pp.1575-1579
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• 2015

Some microRNAs (miRNAs) have been shown to act as oncogenes or tumor suppressor genes in human melanomas. miR-328 is upregulated in blood cells of melanoma patients compared to in healthy controls. This suggests a role for miR-328 in melanoma that warrants investigation. In this study, we demonstrated miR-328 levels to be dramatically decreased in human melanoma cell lines. Moreover, forced expression of miR-328 inhibited proliferation and induced G1-phase arrest of the SK-MEL-1 melanoma cell line. We identified TGFB2 as a direct target gene for miR-328 using a fluorescent reporter assay and western blotting. Levels of TGFB2 were dramatically increased in human melanoma cell lines and were inversely correlated with the miR-328 expression level. Our findings provide new insights into the mechanisms of human melanoma development, indicating that miR-328 has therapeutic potential for this disease.

• 한국컴퓨터정보학회논문지
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• 제13권6호
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• pp.211-216
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• 2008

휴대전화를 통해서 양질의 DMB(Digital Multimedia Broadcasting) 서비스를 받기 위하여 지하나 건물사이 음영지역을 제거하기 의한 갭필러(Gap filler) 안테나가 필요하다. 동축 급전선과 4개의 임피던스 정합회로를 필요로 하는 종래의 방식에 비하여 PCB의 피딩 라인에 의한 위상 차이를 이용한 Quadrifilar Helical Antenna(QHA)를 설계하고 제작한다. MicroWave Studio를 이용한 시뮬레이션을 통하여 기존 방식의 QHA에 비해 안테나의 크기와 직경이 안테나 특성에 많은 영향을 주는 것을 파악하여 QHA 제작에 활용하였다. 시뮬레이션을 통한 설계 데이터를 근거로 별도의 임피던스 정합회로가 필요 없는 QHA를 제작하여, 전기적 특성을 측정한 결과 설계치와 유사한 특성을 얻었다.

• 센서학회지
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• 제30권3호
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• pp.170-174
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• 2021

As the rising attention to the medical and healthcare issue, Bio-MEMS (Micro electro mechanical systems) platform such as bio sensor, cell culture system, and microfluidics device has been studied extensively. Bio-MEMS platform mostly has high resolution structure made by biocompatible material such as polydimethylsiloxane (PDMS). In addition, three dimension structure has been applied to the bio-MEMS. Lithography can be used to fabricate complex structure by multiple process, however, non-rectangular cross section can be implemented by introducing optical apparatus to lithography technic. X-ray lithography can be used even for sub-micron scale. Here in, we demonstrated lines with round shape cross section using the tilted gold absorber which was deposited on the oblique structure as the X-ray mask. This structure was used as a mold for PDMS. Molded PDMS was applied to the cell culture platform. Moreover, molded PDMS was bonded to flat PDMS to utilize to the sub-micro channel. This work has potential to the large area bio-MEMS.

• 한국작물학회지
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• 제50권5호
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• pp.346-355
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• 2005

To investigate the application of biochemical markers' and small-sample methods using whole-wheat flours for screening in early generation in Korean wheat breeding system, 74 Korean wheats, including cultivars, local breeding lines and experimental lines, were analyzed. Seed storage protein and amylose contents of grains were evaluated. Biochemical makers, including granule bound starch synthase (GBSS), high molecular weigh glutenin subunits (HMW-GS) and friabilin were also evaluated by using one-dimensional sodium dodecyl sulfate-polyacryla-mide gel electrophoresis with a single kernel. The small­sample methods, including modified SDS-sedimentation test (MST), micro-alkaline water retention capacity (AWRC) and whole-wheat flour swelling volume (WSV) were also tested in this study. Protein content, MST and AWRC was $11.0 - 15.8\%$, 2.7 - 26.2 ml and $71.9 - 109.7\%$, respectively. Apparent and total amylose content and WSV was $20.6 - 25.0\%$, $26.1 - 32.4\%$ and 9.0 - 16.9 ml, respectively. There were highly significant correlations between MST and AWRC (r=0.592, P<0.001), but Korean wheats showed no significant difference in protein content, amylose content and small-sample methods. In the biochemical markers, Korean wheats contained all three GBSS encoded by Wx loci, except for Suwon 252. Korean wheats showed the high frequency ($58.1\%$) of 1Dx2.2 + 1Dy12 subunits of HMW-GS. Friabilin band was present in 46 lines ($62.2\%$) and absent in 28 lines ($37.8\%$). Friabilin-absence lines showed the higher MST (14.9 ml) and AWRC ($92.1\%$) value than friabilin-presence lines (8.5 ml and $82.4\%$, respectively).

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.1739-1744
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• 2016

We report herein an in vitro anticancer evaluation of a series of seven curcumin analogues (3a-g). The National Cancer Institute (NCI US) Protocol was followed and all the compounds were evaluated for their anticancer activity on nine different panels (leukemia, non small cell lung cancer, colon cancer, CNS cancer, melanoma, ovarian cancer, renal cancer, prostate cancer and breast cancer) represented by 60 NCI human cancer cell lines. All the compounds showed significant anticancer activity in one dose assay (drug concentration $10{\mu}M$) and hence were evaluated further in five dose assays (0.01, 0.1, 1, 10 and $100{\mu}M$) and three dose related parameters $GI_{50}$, TGI and $LC_{50}$ were calculated for each (3a-g) in micro molar drug concentrations (${\mu}M$). The compound 3d (NSC 757927) showed maximum mean percent growth inhibition (PGI) of 112.2%, while compound 3g (NSC 763374) showed less mean PGI of 40.1% in the one dose assay. The maximum anticancer activity was observed with the SR (leukemia) cell line with a $GI_{50}$ of $0.03{\mu}M$. The calculated average sensitivity of all cell lines of a particular subpanel toward the test agent showed that all the curcumin analogues showed maximum activity on leukemia cell lines with $GI_{50}$ values between 0.23 and $2.67{\mu}M$.

• 비파괴검사학회지
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• 제28권2호
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• pp.131-136
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• 2008

정재초음파를 이용하면 유체에 잠겨 있거나 유체를 따라서 흐르는 미세 입자의 조작이 가능하다. 정재초음파 장이 입자에 힘을 작용하여 입자를 음압마디 또는 반음압마디로 이동시킨다. 본 연구에서는 정재초음파의 주파수를 조정함으로써 유동 중 미세 입자의 위치를 제어하는 방법을 제안하고자 한다. 이를 위해 먼저 수침형 초음파 트랜스듀서를 이용하여 폭 수 밀리미터의 미세 채널을 가지는 정재초음파 발생시스템을 구성하였다. 제안한 발생시스템을 이용하여 주파수 2 MHz부터 2.5 MHz까지 영역에서 정재초음파 장을 발생시키고, 물을 따라 흐르는 수 마이크로미터 내외의 탄화규소 입자가 음압 마디로 잘 이동함을 확인하였다. 이때, 미세 채널의 폭과 주파수가 입자의 거동에 미치는 영향을 관찰하였으며, 주파수가 미세입자의 이동 위치를 결정하는 중요한 파라미터임을 확인하였다. 결과적으로, 초음파의 주파수를 조정함으로써 입자의 이동 위치를 제어할 수 있음을 실험을 통해 확인하였으며 최대 범위는 약 261 마이크로미터이다. 본 연구로부터 유체내의 입자 조작에 있어서 정재초음파의 다양한 응용 가능성을 확인할 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권5호
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• pp.1839-1843
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• 2015

Background: MicroRNAs are a class of noncoding RNAs which regulate multiple cellular processes during tumor development. The purpose of this report is to investigate the clinicopathological and prognostic significance of miR-218 in human gliomas. Materials and Methods: Quantitative RT-PCR (qRT-PCR) was conducted to detect the expression of miR-218 in primary normal human astrocytes, three glioma cell lines and 98 paired glioma and adjacent normal brain tissues.Associations of miR-218 with clinicopathological variables of glioma patients were statistically analyzed. Finally, a survival analysis was performed using the Kaplan-Meier method and Cox's proportional hazards model. Results: The expression level of miR-218 in primary normal human astrocytes was significantly higher than that in glioma cell lines (p<0.01). Also, the expression level of miR-218 in glioma tissues was significantly downregulated in comparison with that in the adjacent normal brain tissues (p<0.001). Statistical analyses demonstrated that low miR-218 expression was closely associated with advanced WHO grade (p=0.002) and low Karnofsky performance score (p=0.010) of glioma patients. Kaplan-Meier analysis with the log-rank test showed that patients with low-miR-218 expression had poorer disease-free survival and overall survival (p=0.0045 and 0.0124, respectively). Multivariate analysis revealed that miR-218 expression was independently associated with the disease-free survival (p=0.009) and overall survival (p=0.004) of glioma patients. Conclusions: Our results indicate that miR-218 is downregulated in gliomas and that its status might be a potential valuable biomarker for glioma patients.

• 한국작물학회지
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• 제51권5호
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• pp.458-465
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• 2006

Quality protein maize(QPM) 육성계통의 화학적 특성과 지방산 및 아미노산 조성을 검토하기 위하여 KS5/QPM 계통의 $BC_{1}F_{2}$ 및 KS135/QPM 계통의 $BC_{1}F_{2}$ 자식계통을 대상으로 opaque-2 specific micro-satellite marker인 umc1066를 사용하여 QPM 26계통을 선발하고 non-QPM 34계통을 공시하여 얻어진 결과를 요약하면 다음과 같다. 1. QPM계통은 non-QPM계통에 비하여 지방함량이 유의하게(p<0.05) 증가되었으나 단백질함량은 통계적 유의차가 없었다. 2. QPM 계통과 non-QPM 계통 모두 linoleic acid(Cl8:2)의 조성비가 가장 높고 oleic acid(Cl8:1), palmitic acid(Cl6:0), linolenic acid(Cl8:3), stearic acid(Cl8:0)의 순으로 지방산 조성비가 높았다. 3. QPM 계통은 non-QPM 계통에 비하여 포화지방산의 비율이 다소 높고 불포화지방산의 비율은 non-QPM 계통에 비하여 조성비가 낮았으나 통계적 유의성은 없었다. 4. QPM 계통은 non-QPM 계통에 비하여 lysine의 조성 비율이 높고 황함유 아미노산(cystine, methionine)의 비율이 증가되었다. 5. QPM 계통의 필수아미노산의 조성비율은 28.1%였고 non-QPM 계통은 27.1%로 QPM 계통이 영양학적인 면에서 유리한 것으로 판단되었다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2009년도 하계학술대회 논문집
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• pp.90-91
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• 2009

We study of the variation on non-uniform plasma in different layer of the semiconductor. The transient response in different plasma layer has been evaluated theoretically. The reflection function of dielectric microstrip lines resulting from the presence of plasma are evaluated by the transmission line model. The diffusion length is small compared to the absorption depth. The variation of characteristic response in plasma layer with microwave pulses which has in localized has been evaluated.

• 센서학회지
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• 제20권2호
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• pp.77-81
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• 2011

This paper presents a single-pole eight-throw(SP8T) switch based on proposed a radio-frequency(RF) microelectromechanical systems (MEMS) switches. The proposed switch was driven by a double stop(DS) comb drive, with a lateral resistive contact. Additionally, the proposed switch was designed to have tapered signal line and bi-directionally actuated. A forward actuation connects between signal lines and contact part, and the output becomes on-state. A reverse actuation connects between ground lines and contact part, and the output becomes off-state. The SP8T switch of 3-stage tree topology was developed based on an arrangement of the proposed RF MEMS switches. The developed SP8T switch had an actuation voltage of 12 V, an insertion loss of 1.3 dB, a return loss of 15.1 dB, and an isolation of 31.4 dB at 6 GHz.