• Journal of Biomedical Engineering Research
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• v.37 no.5
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• pp.195-207
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• 2016

This study examined the effectiveness of micro-current stimulation (MCS) to improve the facial skin qualities by performing clinical test. The MCS is generally known that provide healing response in the damaged tissue and pain relief through activating the adenosine triphosphate (ATP) and protein synthesis. In here, we can hypothesize that the improvement of facial skin qualities may occur due to MCS, since the induction of micro-current from outside may enhance the cellular activity according to ATP activation. From the clinical test, our results showed that a variety of evaluating categories, which is able to assess the skin qualities, significantly enhanced due to stimulation of micro-current after 7 and 14 days. Therefore, we can estimate that MCS in human facial skin may be effective to improve the skin qualities.

• The Journal of Korean Physical Therapy
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• v.16 no.3
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• pp.9-21
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• 2004

The purpose of study is that we will observe the change of c-fos with the immunohistochemistry method and then we will study the effect of micro current stimulation following the frequency after inducing pain to rats with capsaicin. Rat were classified to SD and they have growed for 8 weeks. We classify to four groups, ordinal group is used in experiment I, the group which we induce pain is used in experiment II, the application group which we induce pain and then the high frequency micro current stimulation is used in experiment III, the application group which we induce pain and then the low frequency micro current stimulation is used in experiment IV, we get the following result. Compare with experiment II, experiment III, and experiment IV from acute pain modal in the immunohistochemistry experiment which has c-fos protein as an antigen, c-fos immunoreactive positive neurons significantly after induced pain for two hours(p<0.001). According to these results, from rats induced pain, micro current stimulation effect to reducing pain, but following frequency micro current stimulation theraphy isn't different from immunoreactive c-fos

• Journal of Biomedical Engineering Research
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• v.39 no.6
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• pp.284-296
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• 2018

Obesity is considered as a primary health problem over the past century in line with life environmental changes. It is mainly associated with increased risk of numerous chronic diseases which may significantly reduce health-related quality of life. Therefore, efforts to reduce weight should be performed. This study suggested a novel approach to reduce body fat by applying external stimulation which is micro-current stimulation (MCS). In this clinical study, we evaluated the potential effects of MCS for reduction of abdominal fat and weight loss. Prior to the clinical test, computational simulation was conducted to find the proper MCS conditions that allow externally applied stimulation to reach the internal fat section from the external skin. Particularly, the clinical study evaluated the unilateral effects of MCS for body fat loss and lipolysis without any additional limitations such as physical exercise and dietary therapy. The results showed that whole body fat, waist circumferences, and abdominal fat are gradually decreased after intervention in proportion to the time. From the results, we can estimate that MCS can be effective on the body fat loss by activation of lipolysis in human adipose.

• Journal of Biomedical Engineering Research
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• v.40 no.2
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• pp.68-74
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• 2019

Lateral epicondylitis is caused by repeated use of the wrist, which causes inflammation and pain in the wrist extensor and tendon of the humerus. Delayed onset muscle soreness (DOMS) caused by repetitive resistance exercise affects the tendons connected in series with the muscle, leading to lateral epicondylitis. Although micro-current stimulation has been suggested as a possible treatment for tendinitis, there are insufficient studies on specific variables such as frequency. In this study, 15 healthy adult males and females developed DOMS in the wrist extensor and tendon in the humerus. The experimental group consisted of a low frequency group applying 20 Hz and a high frequency group applying 100 Hz according to the micro-current frequency. Each subject underwent an experiment for 5 days after DOMS, and the recovery rates were compared by measuring AROM, GPT, MST, PPT, and VAS. As a result, the 20 Hz group showed significant changes in AROM, MST, and VAS compared to the control group on the 4th day, and the recovery rate was also higher than that of the 100 Hz group. On the 5th day, recovery rate of 100 Hz group was higher than 20 Hz in AROM and PPT, and MST showed higher recovery rate than 20 Hz group, but there was no significant difference. These results indicate that microcurrent stimulation is effective for the treatment of delayed myalgia and tendon inflammation and that the 100 Hz group has faster recovery than the 20 Hz group.

• Journal of Biomedical Engineering Research
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• v.41 no.6
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• pp.235-246
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• 2020

This study aimed to evaluate the inhibitory effect of micro-current stimulation(MCS) on adipogenesis regarding with Wnt/β-catenin pathway using the ob/ob mouse and 3T3-L1 cell line. 6-week old ob/ob male mice were equally assigned to four groups: obese group(ob), obese with MCS groups(50 μA, 200 μA, and 400 μA). 6-week old C57BL/6J male mice were assigned to the control group(CON). We analyzed abdominal adipose tissue volume by using in vivo micro-CT and measured the body weight, feed intake, liver weight and triglycerides in serum. All the MCS groups showed that significantly reduced body weight and triglycerides in serum. In the case of liver weight and abdominal adipose tissue volume, the inhibitory effect of adipogenesis was shown in the 200 μA and 400 μA groups. To elucidate the anti-obesity effect of MCS, β-catenin, C/EBPα and FAS protein expressions were analyzed by western blotting. β-catenin expression was upregulated, C/EBPα and FAS expression were down-regulated in the relatively high-intensity groups(200 μA and 400 μA). Thus, the 200 μA and 400 μA for the intensity of MCS were chosen for cell experiments. In the 3T3-L1 cell line, Wnt/β-catenin pathway including Wnt10b, Wnt3a, β-catenin and Cyclin D1 was activated in all MCS groups. Accordingly, the expression level of C/EBPα was decreased during the differentiation and lipid droplet was significantly reduced in Oil red O staining results. These results suggest that the Wnt/β-catenin signaling might be activated by MCS with current intensities between 200-400 μA and it may lead to anti-obesity effects.

• Journal of The Korean Society of Integrative Medicine
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• v.3 no.2
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• pp.9-16
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• 2015

Purpose: The purpose of this research was to ascertain the effect of Micro-current on a pain, balance, of knee joint in the patients with degenerative arthritis. Method: The 30 subjects who had micro-current in this research and randomly assigned into two group, an experiment group and a control group. micro-current was applied to 15 subject in the experiment group after general physical therapy. experiment group was applies general therapies and micro-current stimulation. The measurement were analyzed by using SPSS(V.20). In order to compare the post to the amount of pre-changes and post-changes of each group, the paired t-test was used. The difference between the experiment group and the control group was analyzed by using the analysis of convariance. Results: The results of this study were as follows; 1) In VAS measures, the pain point was significantly decreased in both the experimental and the control group. 2) Mc Gill Pain Questionnaire(MPQ), the pain point was significantly decreased in both the experimental and the control group. 3) TUG measures, the second was significantly decreased in both the experimental and the control group. 4) Weight distribution measures, the authority was significantly decreased in both the experimental and the control group. Conclusion: The micro-current is considered a degenerative knee arthritis that can be presented as an effective physical therapy intervention.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.521-524
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• 2009

Response to nerve stimulation platform for implementing measures to detect finger movement has been functioning as an important factor. This stimulated finger on the nerve and muscle responses would vary. In other words, the finger movement of the muscle response to nerve stimulation and sensing Actuator for the H/W development is needed. In addition, a low power embedded CPU based on the top was used. H/W configuration portion of the isolation power, constant current control, High impedance INA, amplifier parts, and the stimulus mode and the Micro-control the status of current, AD converter Low Data obtained through the processing system is implemented.

• Journal of Biosystems Engineering
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• v.38 no.2
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• pp.113-120
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• 2013

Purpose: Stem cells provide new opportunities in the regenerative medicine for human or animal tissue regeneration. In this study, we report an efficient method for the modulating behaviors of electro-active stem cells by micro-electric current stimulation (mES) without using chemical agents, such as serum or induction chemicals. Methods: Dental pulp stem cells (DPSCs) were cultured on the tissue culture dish in the mES system. To find a suitable mES condition to promote the DPSC functions, the response surface analysis was used. Results: We found that a working micro-current of 38 ${\mu}A$ showed higher DPSC proliferation compared with other working conditions. The mES altered the expressions of intracellular and extracellular proteins compared to those in unstimulated cells. The mES with 38 ${\mu}A$ significantly increased osteogenesis of DPSCs compared with ones without mES. Conclusions: Our findings indicate that mES may induce DPSC proliferation and differentiation, resulting in applying to DPSCs-based human or animal tissue regeneration.

• Journal of Biomedical Engineering Research
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• v.43 no.1
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• pp.1-10
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• 2022

Non-alcoholic fatty liver disease(NAFLD) is excessive hepatic lipid accumulation mainly caused by obesity. This study aimed to evaluate whether micro-current stimulation(MCS) could modulate lipid metabolism regarding the Sirt1/AMPK pathway, fatty acid β-oxidation pathway, and lipolysis and lipogenesis-related factors in FL83B cells. For the NAFLD cell model, FL83B cells were treated with oleic acid for lipid accumulation. MCS were stimulated for 1 hr and used frequency 10 Hz, duty cycle 50%, and biphasic rectangular current pulse. The intensity of MCS was divided into 50, 100, 200, and 400 ㎂. Through the results of Oil red O staining, it was confirmed that MCSs with the intensity of 200 ㎂ and 400 ㎂ significantly reduced the degree of lipid droplet formation. Thus, these MCS intensities were applied to western blot analysis. Western blot analysis was performed to analyze the effects of MCS on lipid metabolism. MCS with the intensity of 400 ㎂ showed that significantly activated the Sirt1/AMPK pathway, a key pathway for regulating lipid metabolism in hepatocytes, and fatty acid β-oxidation-related transcription factors. Moreover, it activated the lipolysis pathway and suppressed lipogenesis-related transcription factors such as SREBP-1c, FAS, and PPARγ. In the case of MCS with the intensity of 200 ㎂, only PGC1α and SREBP-1c showed significant differences compared to cells treated only with oleic acid. Taken together, these results suggested that MCS with the intensity of 400 ㎂ could alleviate hepatic lipid accumulation by modulating lipid metabolism in hepatocytes.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.6
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• pp.1505-1510
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• 2010

Response to nerve stimulation platform for implementing measures to detect finger movement has been functioning as an important factor. The finger movement of the muscle response to nerve stimulation and sensing Actuator for the H/W development is needed. In addition, a low power embedded CPU based on the top was used. H/W configuration portion of the isolation power, constant current control, High impedance INA, amplifier parts, and the stimulus mode and the Micro-control the status of current, AD converter Low Data obtained through the processing system is implemented.