• 제목/요약/키워드: Micro-Propagation

검색결과 241건 처리시간 0.026초

철피석곡의 기내 Protocorm Like Bodys(PLBs) 재증식 및 신초형성에 미치는 생장조절제 및 탄소원의 영향 (Effect of plant growth regulators and carbon sources on proliferation and shoot formation of PLBs in Dendrobium candidum)

  • 장지우;김창길;;이도진;정미영
    • Journal of Plant Biotechnology
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    • 제46권1호
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    • pp.9-16
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    • 2019
  • 본 연구는 철피석곡의 PLB 기내 대량증식 방법에 대한 기초자료를 제공함으로써 다양한 분야의 소재활용 수요를 충족시키고 아울러 멸종위기에 처해 있는 철피석곡을 보존하고 수행하였다. 종자유래의 PLB 재 증식에는 H3P4 기본배지에 NAA $0.1mg{\cdot}L^{-1}$과 kinetin $0.1mg{\cdot}L^{-1}$를 혼용한 배지가 가장 적합하였다. H3P4 기본배지 내 탄소원으로 sucrose $10g{\cdot}L^{-1}$를 첨가 했을 때 PLB의 생장이 가장 양호하였다. 재 증식된 PLB로부터 shoot 형성율은 1/4MS 또는 H1P2 배지에 sucrose를 $10g{\cdot}L^{-1}$ 첨가한 배지에서 가장 높았고 shoot의 초장도 길어졌다.

초음파 나노표면 개질처리를 통한 베어링강의 회전접촉피로 및 잔류응력 특성에 대한 연구 (Rolling Contact Fatigue and Residual Stress Properties of SAE52100 Steel by Ultrasonic Nano-Crystalline Surface Modification (UNSM))

  • 이창순;박인규;조인식;홍정화;지태구;편영식
    • 열처리공학회지
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    • 제21권1호
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    • pp.10-19
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    • 2008
  • To investigate the effect of ultrasonic nano-crystalline surface modification (UNSM) treatment on rolling contact fatigue and residual stress properties of bearing steels, this paper carried out a rolling contact fatigue test, measured residual stress and retained austenite, performed a wear test, observed microstructure, measured micro hardness, and analyzed surface topology. After the UNSM treatment, it was found that the surface became minute by over $100{\mu}m$. The micro surface hardness was changed from Hv730~740 of base material to Hv850~880 with about 20% improvement, and hardening depth was about 1.3 mm. The compressive residual stress was measured as high as -700~-900 MPa, and the quantity of retained austenite was reduced to 27% from 34%. The polymet RCF-6 ball type rolling contact fatigue test showed over 4 times longer fatigue lifetime after the UNSM treatment under 551 kgf load and 8,000 rpm. In addition, this paper observed the samples, which went through the rolling contact fatigue test, with OM and SEM, and it was found that the samples had a spalling phenomenon (the race way is decentralized) after the UNSM treatment. However, before the treatment, the samples had excessive spalling and complete exploration. Comparison of the test samples before and after the UNSM treatment showed a big difference in the fatigue lifetime, which seems to result from the complicated effects of micro particles, compressive residual stress, retained austenite, and surface topology.

디지털 시그널 프로세서를 이용한 스카라 로봇의 적응-신경제어기 설계 (Design of Adaptive-Neuro Controller of SCARA Robot Using Digital Signal Processor)

  • 한성현
    • 한국생산제조학회지
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    • 제6권1호
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    • pp.7-17
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    • 1997
  • During the past decade, there were many well-established theories for the adaptive control of linear systems, but there exists relatively little general theory for the adaptive control of nonlinear systems. Adaptive control technique is essential for providing a stable and robust performance for application of industrial robot control. Neural network computing methods provide one approach to the development of adaptive and learning behavior in robotic system for manufacturing. Computational neural networks have been demonstrated which exhibit capabilities for supervised learning, matching, and generalization for problems on an experimental scale. Supervised learning could improve the efficiency of training and development of robotic systems. In this paper, a new scheme of adaptive-neuro control system to implement real-time control of robot manipulator using digital signal processors is proposed. Digital signal processors, DSPs, are micro-processors that are developed particularly for fast numerical computations involving sums and products of variables. The proposed neuro control algorithm is one of learning a model based error back-propagation scheme using Lyapunov stability analysis method. The proposed adaptive-neuro control scheme is illustrated to be an efficient control scheme for implementation of real-time control for SCARA robot with four-axes by experiment.

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중탄소강의 피로크랙 개폐구의 특성 및 피로강도의 평가 (Evaluation of Fatigue Strength and Characteristics of Fatigue Crack Closure in SM35C Steel)

    • 한국생산제조학회지
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    • 제6권1호
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    • pp.45-50
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    • 1997
  • It is not clearly known how surface defects or inclusions of a medium carbon steel affect a fatigue strength. In this study, we used SM35C specimens with spheroidized cementite structure to eliminate dependence of micro structure of fatigue crack. The investigation was carried out by behavior of crack closure at non-propagation crack and effect of the fatigue limit according to the artificial defects size. Experimental findings are obtained as follows : (1) Fatigue crack initiation point of medium carbon steel with spheroidized cementite structure is at the surface defects. (2) Non-propagating crack length of smooth specimen is equal to the critical size of defect. (3) Considering the opening and closure behavior of fatigue crack, the defect shape results in various crack opening displacement, while it does not affects the fatigue limit level of medium carbon steel with spheroidized cementite structure. (4) The critical length of the non-propagation crack of smooth specimen is the same as critical size of defect in transient area which determines threshold condition in steel with spheroidized cementite structure.

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자동차 흡기계 공기 여과기 필터의 음향학적 모델 (Acoustic modeling of an air cleaner filter in the engine intake system)

  • 이정권;강장훈
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2006년도 춘계학술대회논문집
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    • pp.114-117
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    • 2006
  • The air filter in engine intake system has a function of filtrating the dirt in the scavenging air as well as attenuating the noise. The noise attenuation within the air cleaner filter, however, has been regarded as negligible by the field engineers. In this paper, for the analysis of the acoustical performance of air filter, an acoustical model was suggested and the characteristics of air filter system were investigated. Fibrous structure of the filter element was modeled as a micro-perforated panel using the flow resistivity and porosity. The pleated geometry of the filter element was modeled as two coupled ducts that have permeable walls, in which each duct area was assumed being constant. Using such simplified geometry, a mathematical model was developed for the sound propagation within a narrow duct system. Visco-thermal effect was considered in modeling the sound propagation through such tubes; the filter box was modeled as a rigid rectangular box. By combining two models, a four-pole transfer matrix was derived. For the validation purpose, transmission loss was measured for a plastic rectangular box containing an air filter. A noticeable effect of the air filter element was observed by including the filter into the box. Comparing the predicted and measured data, we found that the predicted TL agrees well with experimental results, in particular, in magnitude and frequency at TL troughs.

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Time-Varying Seismogenic Coulomb Electric Fields as a Probable Source for Pre-Earthquake Variation in the Ionospheric F2-Layer

  • Kim, Vitaly P.;Hegai, Valery V.;Liu, Jann Yenq;Ryu, Kwangsun;Chung, Jong-Kyun
    • Journal of Astronomy and Space Sciences
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    • 제34권4호
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    • pp.251-256
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    • 2017
  • The electric coupling between the lithosphere and the ionosphere is examined. The electric field is considered as a timevarying irregular vertical Coulomb field presumably produced on the Earth's surface before an earthquake within its epicentral zone by some micro-processes in the lithosphere. It is shown that the Fourier component of this electric field with a frequency of 500 Hz and a horizontal scale-size of 100 km produces in the nighttime ionosphere of high and middle latitudes a transverse electric field with a magnitude of ~20 mV/m if the peak value of the amplitude of this Fourier component is just 30 V/m. The time-varying vertical Coulomb field with a frequency of 500 Hz penetrates from the ground into the ionosphere by a factor of ${\sim}7{\times}10^5$ more efficient than a time independent vertical electrostatic field of the same scale size. The transverse electric field with amplitude of 20 mV/m will cause perturbations in the nighttime F region electron density through heating the F region plasma resulting in a reduction of the downward plasma flux from the protonosphere and an excitation of acoustic gravity waves.

The effect of particle size on the edge notched disk (END) using particle flow code in three dimension

  • Haeri, Hadi;Sarfarazi, Vahab;Zhu, Zheming;Marji, Mohammad Fatehi
    • Smart Structures and Systems
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    • 제22권6호
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    • pp.663-673
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    • 2018
  • In this study, the effect of particle size on the cracks propagation and coalescence or cracking pattern of the edge notched disc specimens are investigated. Firstly, calibration of PFC3D was performed using Brazilian experimental test output. Then micro parameters were used to build edge notched disc specimen. The horizontal wall of the assembly is let to move downward with a standard low speed of 0.016 m/s. The numerical results show that the tensile cracks are dominant failure pattern for the modeled discs. These tensile cracks initiate from the pre-existing notch tip and propagate parallel to the loading direction then interact with the upper boundary of the modeled specimen. As the size of the balls (ball diameter) decrease the number of tensile cracks increase. The tensile fracture toughness of the samples also decreases as the particle size increases. Understanding the crack propagation and crack coalescence phenomena in brittle materials such as concretes and rocks is of paramount importance in the stability analyses for engineering structures such as rock slopes, underground structures and tunneling.

Enhancing the ability of strain energy release rate criterion for fracture assessment of orthotropic materials under mixed-mode I/II loading considering the effect of crack tip damage zone

  • Khaji, Zahra;Fakoor, Mahdi
    • Steel and Composite Structures
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    • 제44권6호
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    • pp.817-828
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    • 2022
  • In this study, considering dissipated energy in fracture process zone (FPZ), a novel criterion based on maximum strain energy release rate (SER) for orthotropic materials is presented. General case of in-plane loading for cracks along the fibers is assumed. According to the experimental observations, crack propagation is supposed along the fibers and the reinforcement isotropic solid (RIS) concept is employed as a superior model for orthotropic materials. SER in crack initiation and propagation phases is investigated. Elastic properties of FPZ are extracted as a function of undamaged matrix media and micro-crack density. This criterion meaningfully links between dissipated energy due to toughening mechanisms of FPZ and the macroscopic fracture by defining stress intensity factors of the damaged zone. These coefficients are used in equations of maximum SER criterion. The effect of crack initiation angle and the damaged zone is considered simultaneously in this criterion and mode II stress intensity factor is extracted in terms of stress intensity factors of damage zone and crack initiation angle. This criterion can evaluate the effects of FPZ on the fracture behavior of orthotropic material. Good agreement between extracted fracture limit curves (FLC's) and available experimental data proves the ability of the new proposed criterion.

오옥신과 사이토키닌 호르몬 처리를 이용한 칼라 기내 식물체 대량증식체계 확립 (Establishment of propagation system for in vitro calla plants(Zantedeschia spp.) by using auxin and cytokinin hormones treatments)

  • 이상희;김영진;양환래;김종보
    • 문화기술의 융합
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    • 제4권4호
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    • pp.325-329
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    • 2018
  • 천남성과에 속한 칼라는 생육 조건에 따라 두 종류로 구분이 가능한데 그 중 꽃 모양과 화색이 다양한 유색 칼라는 절화로서 인기가 매우 높다. 그러나 유색 칼라는 자연 번식률이 매우 낮고 개화구근을 얻기 까지 오랜 시간이 소요되어 우량 품종의 대량 번식 체계 확립이 시급하다. 따라서 본 연구에서는 칼라 기내 식물체의 생육을 향상시키고자 다양한 식물 성장 호르몬을 처리하여 실험을 진행하였다. 뿌리 발생은 기본 MS 배지에서 81.5 %로 가장 높았고 신초 및 다신초 발생률은 BA 2.0 mg/L와 IBA 1.0 mg/L 혼용 처리구에서 100.0 %와 36.4 %로 가장 높았다. 또한 생체중에서는 BA 2.0 mg/L 단일 처리구에서 11.2배가 증가하여 모든 처리구중 가장 높은 증가율을 보였다. Auxin과 cytokinin 혼용 처리 실험은 이전 연구에서도 많이 이용되고 있고 칼라 식물 생장에도 긍정적인 효과를 줌으로써 미소대량증식체계 확립에 도움을 줄 것이라고 판단된다.

부유 및 퇴적의 분체 조건이 화재폭발 특성에 미치는 영향 (Effect of Powder Condition on the Fire and Explosion Characteristics of Suspended and Deposited Dusts)

  • 한우섭;서동현;최이락;임진호
    • Korean Chemical Engineering Research
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    • 제60권2호
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    • pp.229-236
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    • 2022
  • 동일 분체특성의 분진이 평균입경, 농도, 분진조건(부유 또는 퇴적) 변화에 따른 화재폭발 위험성을 조사하였다. 이를 위해 20L분진폭발시험장치, 열중량분석장치, 연소속도시험장치(UN시험법)를 사용하였다. 4종 분진(Sugar, Mg, Al, Zr)의 입경이 서로 다른 8개 분진 시료에 대하여 부유 분진의 폭발특성과 화염전파속도(FPV), 그리고 퇴적분진의 화염확산속도(FSV)를 조사하였다. 부유 분진 조건에서 Mg 및 Al 분진은 입경이 감소하면 폭발 위험성이 증가하였지만, Sugar는 입경 변화에 따른 폭발 위험성의 영향이 거의 나타나지 않았다. 부유 분진의 화염전파속도(FPV)는 마이크로 범위에서의 입경 변화보다 마이크로에서 나노로 입경이 감소하면 크게 증가하였다. 퇴적층의 화염확산속도(FSV)는 수평면(기울기 0°)보다 경사면(기울기 30°)에서 증가하는 경향을 나타냈으며, 경사면(기울기 30°) 퇴적층 조건에서는 상방 전파가 하방 전파보다 높게 나타났다.