• 한국가시화정보학회:학술대회논문집
• /
• 2002.11a
• /
• pp.45-48
• /
• 2002

To investigate the flow related to the mixing, micro PIV measurements were performed in the middle plane of the channel. A passive micro mixer analyzed in this work has been designed in the shape of a three-dimensional microchannel and fabricated with PDMS molding process by KAIST. The mixing performance was evaluated for different flow rates using phenolphthalein and sodium hydroxide solutions. Results show that mixing is enhanced by the increase of flow rate, which yields stronger secondary flows with helical streamlines.

• Proceedings of the KSME Conference
• /
• 2002.08a
• /
• pp.423-426
• /
• 2002

A custom micro-PIV optics assembly has been used to measure the flow field inside a T-shaped microchannel. The micro-PIV system consists of microscope objectives of various magnifications, a dichroic cube, and an 8-bit CCD camera. Fluorescent particles of diameters 620nm have been used with a Nd:YAG laser and color filters. A programmable syringe pump with Teflon tubings were used to inject particle-seeded distilled water into the channel at flow rates of $420,\;40,\;60{\mu}L/hr$. The microchannels are fabricated with PDMS with a silicon mold, then $O_2-ion$ bonded onto a slide glass. Results show differences in flow characteristics and resolution according to fluid injection rates, and magnifications, respectively. The results show PIV results with vector-to-vector distances of $2{\mu}m$ with 32 pixel-square interrogation windows at $50{\%}$ overlap.

• Journal of the Korean Society of Visualization
• /
• v.15 no.3
• /
• pp.14-19
• /
• 2017

Increase of blood viscosity significantly changes the flow resistance and wall shear stress which are related with cardiovascular diseases. For measurement of blood viscosity, microfluidic method has proposed by monitoring pressure between sample and reference flows in the downstream of a microchannel with two inlets. However, it is difficult to apply this method to unknown flow conditions. To measure blood viscosity under unknown flow conditions, a microfluidic method based on micro particle image velocimetry(PIV) is proposed in this study. Flow rate in the microchannel was estimated by assuming velocity profiles represent mean value along channel depth. To demonstrate the measurement accuracy of flow rate, the flow rates measured at the upstream and downstream of a T-shaped microchannel were compared with injection flow rate. The present results indicate that blood viscosity could be reasonably estimated according to shear rate by measuring the interfacial width and flow rate of blood flow. This method would be useful for understanding the effects of hemorheological features on the cardiovascular diseases.

• Proceedings of the KSME Conference
• /
• 2002.08a
• /
• pp.419-422
• /
• 2002

A three-dimensional inlet flow structure inside a microfluidic element has been investigated using a micro-PIV(particle image velocimetry) measurement as well as a numerical analysis. The present study employs a state-of-art micro-PIV system which consists of epi-fluorescence microscope, 620nm diameter fluorescent seed particles and an 8-bit megapixel CCD camera. For the numerical analysis, a commercial software CFD-ACE+(V6.6) was employed for comparison with experimental data. Fixed pressure boundary condition and a 39900 structured grid system was used for numerical analysis. Velocity vector fields with a resolution of $6.7{\times}6.7{\mu}m$ has been obtained, and the attention has been paid on the effect of varying measurement conditions of particle diameter and particle concentration on the resulting PIV results. In this study, the microfluidic elements were fabricated on plastic chips by means of MEMS processes and a subsequent melding process.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.30 no.4 s.247
• /
• pp.314-319
• /
• 2006

To analyze in-vivo blood flow characteristics in a chicken embryo, in-vivo experiment was carried out using micro-PIV technique. Because endothelial cells in blood vessels are subject to shear stress of blood flow, it is important to get velocity field information of the placental blood flow. Instantaneous velocity fields of an extraembryonic blood vessel using a high-speed camera and intravital microscope. The flow images of RBCs were obtained with a spatial resolution of $20\times20{\mu}m$ in the whole blood vessels. The mean velocity field data confirm that the blood flow does show non-Newtonian fluid characteristic. The blood in a branched vessel merged smoothly without any flow separation into the main blood vessel with the presence of a slight bump. This in-vivo micro-PIV measurement technique can be used as a powerful tool in various blood flow researches.

• 한국전산유체공학회:학술대회논문집
• /
• 2008.03b
• /
• pp.585-588
• /
• 2008

Thermal mixing phenomena in a Y-type microchannel were investigated using a micro-PIV. Two inlet reservoirs of the microchannel were controlled individually so that the characteristics of thermal mixing in the channel with temperature difference were compared with those without the difference. The velocity field in the mixing process was measured using the micro-PIV system that includes an ICCD (Intensified CCD) camera. The mixing area and uniformity were also analyzed. It is observed that the flow fluctuation in spanwise direction is induced by temperature difference, which enhances mixing process in microchannels.

• Journal of the Korean Society of Visualization
• /
• v.8 no.4
• /
• pp.31-37
• /
• 2010

In this study, a novel hybrid micro/nano PIV system combining defocusing and TIRFM technique has been developed for the multiscale flow measurement. With the developed system, both far and near field velocity fields have been measured simultaneously in a 2D straight microchannel and the particle trajectories were extracted by the nearest tracking algorithm. The shear rate values taken from experimental results have been estimated by comparing with the analytical solution of 2D Poiseuille flow and it is confirmed that the result shows good agreement with the theoretical value.

• Journal of the Korean Society of Visualization
• /
• v.3 no.1
• /
• pp.20-25
• /
• 2005

An x-ray PIV (Particle Image Velocimetry) technique was developed fur measuring quantitative information on flows inside opaque conduits and/or opaque-fluid flows. To check the performance of the x-ray PIV technique developed, it was applied to a liquid flow in an opaque Teflon tube. To acquire x-ray images suitable for PIV velocity field measurements, the refraction-based edge enhancement mechanism was employed with seeding detectable tracer particles. The amassed velocity field data obtained were in a reasonable agreement with the theoretical prediction. The x-ray PIV technique was also applied to get velocity fields of blood flow and to measure size and velocity of micro-bubbles simultaneously, and to visualize the water refilling process in bamboo leaves. The x-ray PIV was found to be a powerful transmission-type flow imaging technique fur measuring quantitative information of flows inside opaque objects and various opaque-fluid flows.

• Proceedings of the KSME Conference
• /
• 2004.11a
• /
• pp.1534-1539
• /
• 2004

This experiment has been carried out to measure the process of droplet formation between water phase fluid(PVA 3%) and organic phase fluid(oil) and vector fields measured by a Dynamic Micro-PIV method in the inside of a droplet while generated. Droplet length controlled by changing flow rate conditions in microchannel. Water-in-oil(W/O) droplets successfully generated at a Y junction and cross microchannel. But oil-in-water(O/W) droplets could not be formed at a Y junction microchannel. That is, PVA 3% flow could not be detached from the PDMS surface and ran parallel with oil flow. When PVA 3% flow rate was constant, droplet length and time period decreased as oil flow rate increased, but droplet frequency increased. When PVA 3% and oil flow rate ratio was constant, droplet length and time period decreased as flow rate increased, but droplet frequency increased. All that case, Standard deviation of droplet formation have less than 5% at averaged droplet length and regular-sized droplets were reproducibly formed.

• 한국전산유체공학회:학술대회논문집
• /
• 2008.03b
• /
• pp.105-106
• /
• 2008

As a carrier of malaria and sneak of blood, mosquitoes are an unpleasant insect. However, there are several unknown natural secretes related with mosquitoes. Among them, we focused on the blood sucking process of a female mosquito. The main objective of this study is to understand the mosquito's blood sucking mechanism that can be used to resolve the problem encountered in the injection or transport of infinitesimal biological fluids in a micro-chip. At first, the velocity fields of blood-sucking flow in a proboscis were measured using a micro-particle image velocimetry (PIV) technique. The velocity signals of flow in the proboscis show periodic variation. This seems to be resulted from the beating of the pharyngeal pump which works as driving power. To analyze the pumping mechanism, the temporal variation of the pharyngeal pump was visualized using the synchrotron X-ray micro-imaging technique. The volume variation was estimated by the help of digital image processing techniques. Once the main mechanism of blood sucking process was found, a effective micro-pumping system with high efficiency would be developed in near future.