• Title/Summary/Keyword: Miconazole

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Cytoprotective effect of rhamnetin on miconazole-induced H9c2 cell damage

  • Lee, Kang Pa;Kim, Jai-Eun;Park, Won-Hwan
    • Nutrition Research and Practice
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    • v.9 no.6
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    • pp.586-591
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    • 2015
  • BACKGROUND/OBJECTIVES: Reactive oxygen species (ROS) formation is closely related to miconazole-induced heart dysfunction. Although rhamnetin has antioxidant effects, it remained unknown whether it can protect against miconazole-induced cardiomyocyte apoptosis. Thus, we investigated the effects of rhamnetin on miconazole-stimulated H9c2 cell apoptosis. MATERIALS/METHODS: Cell morphology was observed by inverted microscope and cell viability was determined using a WelCount$^{TM}$ cell proliferation assay kit. Miconazole-induced ROS production was evaluated by fluorescence-activated cell sorting with 6-carboxy-2',7'-dichlorofluoroscein diacetate ($H_2DCF$-DA) stain. Immunoblot analysis was used to determine apurinic/apyrimidinic endonuclease 1 (APE/Ref-1) and cleaved cysteine-aspartic protease (caspase) 3 expression. NADPH oxidase levels were measured using real-time polymerase chain reaction. RESULTS: Miconazole (3 and $10{\mu}M$) induced abnormal morphological changes and cell death in H9c2 cells. Rhamnetin enhanced the viability of miconazole ($3{\mu}M$)-treated cells in a dose-dependent manner. Rhamnetin (1 and $3{\mu}M$) treatment downregulated cleaved caspase 3 and upregulated APE/Ref-1 expression in miconazole-stimulated cells. Additionally, rhamnetin significantly reduced ROS generation. CONCLUSIONS: Our data suggest that rhamnetin may have cytoprotective effects in miconazole-stimulated H9c2 cardiomyocytes via ROS inhibition. This effect most likely occurs through the upregulation of APE/Ref-1 and attenuation of hydrogen peroxide levels.

Selective Combination Effect of Anethole to the Antifungal Activities of Miconazole and Amphotericin B (Miconazole과 Amphotericin B의 항진균 활성에 대한 Anethole의 선택적 병용 효과)

  • 이상화;김창진
    • YAKHAK HOEJI
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    • v.43 no.2
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    • pp.228-232
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    • 1999
  • The combination effect of anethole with amphotericin B, fluconazole, miconazole, or 5-fluorocytosine was investigated against Saccharomyces cerevisiae. When combined with $\frac{1}{2}$ minimum inhibitory concentration (MIC) or $\frac{1}{2}$ minimum fungicidal concentration (MFC) of anethole, the antifungal activities of fluconazole and 5-fluorocytosine were not changed, but the fungistatic and the fungicidal activities of miconazole were increased 64-fold, respectively. In the case of amphotericin B, the fungistatic activity was increased 2-fold, while the fungicidal activity was decreased 2-fold. The combination effect of anethole with miconazole or amphotericin B was also investigated at the various concentrations using the macrobroth dilution checkerboard method. The fractional inhibitory concentration (FIC) and the fractional fungicidal concentration (FFC) index between B exhibited the FIC index of 8.25 and the FFC of 32.06, respectively. Thus, it is analyzed that the combination of anethole with miconazole or amphotericin B on the antifungal action shows synergism and antagonism, respectively.

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Antibacterial Activity of Antimycotic Miconazole against Methicillin-Resistant Staphylococcus aureus

  • LEE, SANG-HWA;CHANG-JIN KIM
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.572-575
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    • 1999
  • Miconazole was estimated to have a minimum inhibitory concentration of 0.78 ㎍/㎖ against the methicillin-resistant strains of Staphylococcus aureus (MRSA). The antibacterial activity of miconazole against MRSA was bacteriostatic in cation-adjusted Mueller-Hinton broth, but bacteriocidal in saline solution. The anti-MRSA activity of miconazole did not show a significant change under the susceptibility-testing conditions such as various inoculum sizes, pHs, or cations (Ca/sup ++/ or Mg/sup ++/) which was added to the medium. However, the anti-MRSA activity of miconazole was completely suppressed by lipophilic a-tocopherol (50 ㎍/㎖), but definitely not by water-soluble ascorbic acid.

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Antifungal susceptibility of Candida spp isolated from bovine mammary glands and teat cups of milking machines (Candida속 균의 항진균성약제에 대한 감수성)

  • Yeo, Sang-geon;Chung, Kyu-young;Cho, Hee-tack
    • Korean Journal of Veterinary Research
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    • v.29 no.1
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    • pp.69-73
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    • 1989
  • In vitro antifungal susceptibility test was carried out on 53 strains of Candida spp isolated from milk of dairy cows with subclinical mastitis and teat cups of milking machines, Nystatin, clotrimazole, miconazole, econazole, 5-fluorocytosine, cycloheximide, haloprogin and griseofulvin were tested by the agar dilution method. The 84.8% to 98.2% of Candida strains were inhibited by clotrimazole, econazole and miconazole at $${\leq_-}25{\mu}g/ml$$, and clotrimazole was most active. Interspecies differences of antifungal susceptibility were recognized and these were as follows. C albicans was most sensitive to clotrimazole (GM-MIC, $5.49{\mu}g/ml$) followed by 5-fluorocytosine, econazole and miconazole. C pseudotropicalis and C guilliermondii were notably sensitive to haloprogin, clotrimazole, miconazole, cconazole, 5-fluorocytosine, and haloprogin (GM-MIC, $0.17{\sim}0.19{\mu}g/ml$) was most active. C krusei was most sensitive to cycloheximide (GM-MIC, $0.54{\mu}g/ml$) followed by clotrimazole, haloprogin, miconazole and econazole. C parapsilosis was somewhat sensitive to econazole, cycloheximide, clotrimazole, and econazole (GM-MIC, $7.26{\mu}g/ml$) was most active. C tropicalis showed very low sensitivity to all tested drugs (GM-MIC, $${\geq_-}20.32{\mu}g/ml$$).

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Synergistic Effect of Polygodial with Imidazole Drugs on the Antifungal Activity (천연물 Polygodial과 Imidazole계 화합물의 병용에 의한 항진균 활성의 증진)

  • 이상화;이재란;김창진
    • YAKHAK HOEJI
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    • v.43 no.2
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    • pp.221-227
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    • 1999
  • The fungistatic and fungicidal activities of amphotericin B, fluconazole, miconazole, econazole, and 5-fluorocytosine against Saccharomyces cerevisiae were estimated in the presence of 1/2 minimum inhibitory concentration (MIC) and 1/2 minimum fungicidal concentration (MFC) of polygodial, respectively. Among them, the antifungal activities of miconazoles by polygodial was still shown against several yeast-like fungi including Candida albicans, Candida utilis, Cryptococcus neoformans, except for Candida krusei. The combination of polygodial with imidazole drugs against Saccharomyces cerevisiae was further examined using the macrobroth dilution checkerboard method. The fractional inhibitory concentration (FIC) and the fractional fungicidal concentration (FFC) index between polygodial and miconazole were 0.16 and 0.19, while the combination of polygodial with econazole exhibited the FIC index of 0.19 and the FFC of 0.25, respectively. These results suggest that polygodial and the imidazoles on the fungistatic and fungicidal action are highly synergistic.

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Isolation of Candida albicans from Denture Patients and in Vitro Activities of Amphotericin B and Miconzole (의치환자에서 Candida albicans분리와 amphotericin B 및 miconazole에 시험관내 감수성)

  • Shin, Moo-Hak;Kim, Shin-Moo
    • Journal of Technologic Dentistry
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    • v.19 no.1
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    • pp.113-120
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    • 1997
  • Candida albicans is now well recognized among the denture stomatitis patients. The broth macrodilution test is the most widely used technique for antifungal susceptibility testing. The purpose of this study was to determine the C. albicans carrier rate of the denture patients in Iksan, chonbuk. To determine the C. albicans carrier rate of denture patients, culture were made from 227 sample taken in Iksan, Chonbuk during July 1997 to August 1997. Also activities of amphotericin B and miconzole against isolates of denture patients of C. albicans were tested by broth macrodilution test using RPMI medium 1640. The results were as follows : First C. ablicans was isolated from 6.6% of denture patients samples and the frequency of isolation fo C. albicans was highest(50%) in the age group of 71-year-old to 80-year-old denture patients. Second, against C. albicans, the MIC range of amphotericn B was $0.06{\sim}0.25{\mu}g/ml$. MIC50 and MIC90 were $0.13{\mu}g/ml$ and $0.25{\mu}g/ml$, respectively. Third, the MIC range of miconazole was $10-{\ge}20{\mu}g/ml$ and MIC50 and MIC90 were $20{\mu}g/ml$ and ${\ge}20{\mu}g/ml$, respectively. It was concluded from this study that C. albicans acrriages from healthy denture individuals only over 60-year-old ages were isolated, they remain susceptible to amphotericin B and not rarely resistant to miconzole.

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Screening of Potential Anticancer Compounds from Marketed Drugs: Aripiprazole, Haloperidol, Miconazole, and Terfenadine Inhibit Cytochrome P450 2J2 (시판 약물의 시토크롬 2J2 약물대사효소 저해능 탐색)

  • Liu, Kwang-Hyeon
    • Journal of Life Science
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    • v.21 no.11
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    • pp.1558-1564
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    • 2011
  • Cytochrome P450 2J2 (CYP2J2) plays important roles in the metabolism of endogenous metabolites such as arachidonic acid as well as therapeutic drugs. CYP2J2 is overexpressed in human cancer tissues and cancer cell lines, as well as in epoxyeicosatrienoic acids (EETs) and CYP2J2-mediated metabolites, and prevent apoptosis of cancer cells. This study aimed to screen marketed drugs for inhibitory potential on CYP2J2 isoforms using human liver microsomes. The initial screen isolated 4 compounds, from 120 marketed drugs, that inhibited the CYP2J2-mediated astemizole O-demethylation more than 50% in the following the order: haloperidol (75%) > terfenadine (56%) > aripiprazole (55%) > miconazole (52%). Miconazole strongly inhibited CYP2J2-mediated ebastine hydroxylation ($IC_{50}$=11.2 ${\mu}M$) and terfenadine hydroxylation ($IC_{50}$=2.2 ${\mu}M$), and terfenadine also inhibited CYP2J2-mediated ebastine hydroxylation ($IC_{50}$=13.6 ${\mu}M$) in a dose dependent manner. The present data suggest that these drugs are potential candidates for further evaluation for their anti-cancer activities.

In vitro Antifungal Activity of 4-Hydroxyderricin and Acetylshikonin against Ascosphaera apis

  • Park, Sangchul;Shin, Yu-Kyong;Cho, MyoungLae;Kwon, Hyun Sook;Kwon, Yun Ju;Kim, Ki-Young
    • Journal of Apiculture
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    • v.34 no.2
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    • pp.125-129
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    • 2019
  • Honey bees are important pollinators in agriculture, but are threatened by the pathogen Ascosphaera apis, which causes chalkbrood. Despite attempts to control this fungus using synthetic fungicides, none of them have been proven to be completely effective. Among 640 natural compounds that we tested, 4-hydroxyderricin (MIC=3.125, 6.25 mg/L after 24 h and 48 h growth, respectively) exhibited the strongest anti- Ascosphaera apis activity, followed by acetylshikonin (MIC=12.5 mg/L for 24 h and 48 h growth). 4-Hydroxyderricin showed selective growth inhibition of Ascosphaera apis and Rhizopus oryzae among tested fungus strains. Treatment 4-hydroxyderricin with miconazole revealed a synergistic effect (FICI=0.65±0.13 at 48 h incubation). These findings suggest that 4-hydroxyderricin, which has antifungal activity against Ascosphaera apis but few other fungal species, can effectively control infectious fungal diseases. Combined treatment of bees with 4-hydroxyderricin and miconazole could reduce cytotoxicity and improve the cost effectiveness of treatment.

Trichophyton Verrucosum Infection in a Camel and its Handler (사슴과 그 취급자에 있어서 Trichophyton verrucosum 감염 1예)

  • 팔마헨드라;이창우
    • Journal of Veterinary Clinics
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    • v.17 no.1
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    • pp.293-294
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    • 2000
  • 6개월령의 숫 낙타와 29세의 남성 낙타 관리인에서 발생한 Trichophyton verrucosum에 의한 진균감염증에 대해 보고하였다. 이 감염증은 피부 병변으로부터 KOH 기술에 의해 균 요소를 증명하고, 또한 감염된 피부의 소파물로부터 Trichophyton verrucosum을 분리하여 확진하였다. 낙타에서는 안면에 국한하여 병변이 발생하고, 관리인에서는 손에 발생하였다. 화학요법으로서 낙타에게는 2% 옥도정기를 사용하고, 관리인에게는 2% miconazole 연고를 사용하였다. 이 보고는 인도에 사는 단봉낙타로부터 Trichophyton verrucosum이 직접 사람에게 전염된 최초의 보고로 생각된다. 이 보고에서 단봉낙타의 피부염 감별진단에 Trichophyton verrucosum도 고려해야 한다는 사실을 제시하였다.

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JNirrofporNm c$\alpha$air Infection in a Horse and its Transmission to Man (말에 있어서 Microsporum canis 감염증과 사람에의 전염)

  • 팔마헨드라;이창우
    • Journal of Veterinary Clinics
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    • v.15 no.1
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    • pp.196-198
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    • 1998
  • 젊은 말과 그 관리인에게 발생한 Microsporum canis 감염에 의한 백선에 대해 보 고하였다. 말과 사람에 있어서의 진단은 피부 병변의 KOH 표본에서 피부사상균을 직접 증명 하고,감염된 인설의 순수 배양물로부터 Microsporum canis를 분리한 것에 근거를 두었다. 말과 사람에 서 분리하고, PHOL 염색액으로 염색한 Microsporum canis는 현미경으로 자세히 관찰했을 때 형태학 적인 차이를 나타내지 않았다. 말에 있어서 옥도정기와 사람에 있어서 miconazole에 의한 국 소 요법은 효과적이었다. 병력은 관리인이 말로부터 감염된 것을 제시하였다.

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