Antifungal susceptibility of Candida spp isolated from bovine mammary glands and teat cups of milking machines

Candida속 균의 항진균성약제에 대한 감수성

In vitro antifungal susceptibility test was carried out on 53 strains of Candida spp isolated from milk of dairy cows with subclinical mastitis and teat cups of milking machines, Nystatin, clotrimazole, miconazole, econazole, 5-fluorocytosine, cycloheximide, haloprogin and griseofulvin were tested by the agar dilution method. The 84.8% to 98.2% of Candida strains were inhibited by clotrimazole, econazole and miconazole at $${\leq_-}25{\mu}g/ml$$, and clotrimazole was most active. Interspecies differences of antifungal susceptibility were recognized and these were as follows. C albicans was most sensitive to clotrimazole (GM-MIC, $5.49{\mu}g/ml$) followed by 5-fluorocytosine, econazole and miconazole. C pseudotropicalis and C guilliermondii were notably sensitive to haloprogin, clotrimazole, miconazole, cconazole, 5-fluorocytosine, and haloprogin (GM-MIC, $0.17{\sim}0.19{\mu}g/ml$) was most active. C krusei was most sensitive to cycloheximide (GM-MIC, $0.54{\mu}g/ml$) followed by clotrimazole, haloprogin, miconazole and econazole. C parapsilosis was somewhat sensitive to econazole, cycloheximide, clotrimazole, and econazole (GM-MIC, $7.26{\mu}g/ml$) was most active. C tropicalis showed very low sensitivity to all tested drugs (GM-MIC, $${\geq_-}20.32{\mu}g/ml$$).

준임상형 유방염 이환 젖소의 유즙과 유두컵으로부터 분리된 Candida속 균의 8종 항진균성약제에 대한 감수성을 조사하였던 결과는 다음과 같다. 총 53주의 Candida속균 중 98.2%, 94.4%, 84.8%의 균주가 각각 clotrimazole, econazole, miconazole의 농도 $25{\mu}g/ml$이하에서 발육이 억제됨으로서, 이들 약제의 항균력이 타 약제에 비하여 우수하였다. 균종별로 보면 C albicans는 clotrimazole에 대한 감수성이 가장 높았으며, 기타 비교적 감수성이 높았던 약제는 5-fluorocytosine, econazole, miconazole의 순이었다. C pseudotropicalis와 C guilliermondii는 haloprogin, clotrimazole, miconazole, econazole, 5-fluorocytosine등에 높은 감수성을 나타내었으며, haloprogin의 평균 MIC가 $0.17{\sim}0.19{\mu}g/ml$로 가장 우수하였다. C krusei는 cycloheximide에 대한 감수성이 가장 높았으며, 그의 비교적 감수성이 높았던 약제는 clotrimazole, haloprogin, miconazole, econazole의 순이었다. 한편 C parapsilosis는 econazole, cycloheximide, clotrimazole등에 다소 감수성을 나타내었으나, 이들 약제의 MIC는 econazole(기하평균 MIC $7.26{\mu}g/ml$)을 제외하고는 타균종에서 보다 현저히 낮았다. 또한 C tropicalis는 전 공시약제에 대하여 감수성이 낮은 경향이었다.