• Korean Journal of Soil Science and Fertilizer
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• v.31 no.1
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• pp.1-8
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• 1998

To compare different methods for the determination of available soil P in the relationship between the soil P and plant uptake of P, rice and corn were cultivated in the pot filled with soils of different available P contents. The soils were taken from 20 rice fields and 20 upland soils. The available soil P were determined before the experiment by Olsen, Lancaster, Bray 1, Bray 2, Mehlich II and Calcium lactate methods and the correlations between the available soil P measured by different methods and the amount of P taken up by corn at 10 leaf stage and by rice at maximum tillering stage were investigated. In upland soils, the available P measured by all the methods was linearly regressed with high significance to the amount of P taken up by corn. The coefficients of determination($R^2$) in the regression equations ranged from 0.596(Calcium lactate method) to 0.891(Olsen method). In rice soils, the soil available P did not regressed by first-order to the amount of P taken up by rice plant, while Michaelis-Menten equation better explained the relationship between the two parameters. In the Michaelis-Menten equation the coefficients of determination for each analytical method ranged from 0.607(Mehlich II method) to 0.923(Lancaster method). Based upon these observations it is concluded that for testing of available P for corn soils Olsen, Bray 2 and Lancaster methods are equally superior to other methods. For rice soils Lancaster, Bray 1, and Bray 2 methods are better than others.

• Bulletin of the Korean Chemical Society
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• v.25 no.7
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• pp.997-1002
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• 2004

A hydrogenperoxide sensor containing peroxidase extracted from horseradish was constructed and pH effect on its sensing ability was investigated. Current profiles of the biosensor with pH and the electrophoretic analysis showed that horseradish peroxidase consists of two isozymes. Assuming that it is a hypothetical twoisozyme mixture, the current profiles were deconvoluted into two Gaussians. Application of the new Michaelis-Menten equation connoting pH concept to this system enabled to find all the related dissociation constants of the isozyme-substrates and the isozyme-proton complexes and to determine pHs for the maximal isozyme activities.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.418-421
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• 2000

A model was developed to describe the microbial decontamination of diesel contaminated soil in a soil column. The biodegradation rate of diesel in nature depends on temperature and the pH of soil, availability of nutrients, oxygen and water. The soil moisture content is one of the essential factors because it characterizes the availability not only of water to microorganisms but also of oxygen and nutrient dissolved in soil. In this work, the rate of biodegradation was modeled by coupling Michaelis-Menten kinetics for the aqueous-phase solute with adsoption-desoption equation for diesel sorption and desorption from soil.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.426-429
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• 2000

Recombinant E. coli JM109(pZH3-5/pMT) harboring manganese transport gene(mntA) and metal sequestering protein, metallothionein(MT), was cultivated to accumulate cadmium in aqueous phase. Bioaccumulation followed Michaelis-Menten type kinetics. Equilibrium isotherm showed Langmuir type isotherm. The optimum pH for $Cd^{2+}$ uptake was 7-7.5.

• Bulletin of the Korean Chemical Society
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• v.34 no.1
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• pp.243-245
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• 2013

Rate equations are exactly solved for the reversible consecutive reaction of the first-order and the time-dependence of concentrations is analytically determined for species in the reaction. With the assumption of pseudo first-order reaction, the calculation applies and determines the concentration of product accurately and explicitly as a function of time in the unimolecular decomposition of Lindemann and in the enzyme catalysis of Michaelis-Menten whose rate laws have been approximated in terms of reactant concentrations by the steady-state approximation.

• Journal of the Korean Chemical Society
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• v.15 no.1
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• pp.11-14
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• 1971

A modified form of the integrated Michaelis-Menten equation would provide a useful means of evaluating enzyme kinetic parameters in nonlinear progress reaction with time. A slight modification of the Lineweaver-Burk form (and other variants) using for the velocity, the change in substrate concentration divided by time ($\={v}$), and for the velocity, the change in substrate for the time interval ($\={S}$), allows this linear reciprocal form to be used with negligible error even when as much as half of the substrate is utilized during the time interval.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.6
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• pp.157-165
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• 2016

Among high-tech products, the mobile phone has experienced a rapid rate of innovation and a shortening of its product life cycle. The shortened product life cycle poses major challenges to those involved in the creation of forecasting methods fundamental to strategic management and planning systems. This study examined whether the best model applies to the entire diffusion life span of a mobile phone. Mobile phone sales data from a specific mobile service provider in Korea from March of 2013 to August of 2014 were analyzed to compare the performance of two S-shaped diffusion models and two non-linear regression models, the Gompertz, logistic, Michaelis-Menten, and logarithmic models. The experimental results indicated that the logistic model outperforms the other three models over the fitted region of the diffusion. For forecasting, the logistic model outperformed the Gompertz model for the period prior to diffusion saturation, whereas the Gompertz model was superior after saturation approaches. This analysis may help those estimate the potential mobile phone market size and perform inventory and order management of mobile phones.

• Journal of environmental and Sanitary engineering
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• v.22 no.4
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• pp.11-21
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• 2007

The recent investigation indicates that the kinetic constants for anionic ions were merely the result of ion exchange between the algae cell wall surface and the anionic ion. In this study, Zygnema sterile and Lepocinclism textra, floating flagellate alga as the dominant algae strains, were cultivated using HRABR(High Rate Algae Biomass Reactor) and the cultivation conditions were 24 hrs. and 12 hrs. irradiation and it was studied how this algal biomass acts on the biosorption mechanism of anionic N and P. Results are as follows : 1. Calculating the specific chl.-a growth rate using Michaelis-Menten model, the one of 24hrs. irradiation was about 55 times higher than the one of 12 hrs. irradiation 2. Calculating the specific chl.-a growth rate using Kuo model, the one of 24 hrs. irradiation was about 2.26 times higher than the one of 12 hrs. irradiation 3. Langmuir model can apply to the biosorption mechanism of anionic N and P in HRABP. 4. Regarding the chlorophyll-a concentration as unit weight of sorbent, the ion selectivity coefficients for N and P are as follows : $(NH_3-N)+(NO_3-N)$ in 24 hrs. irradiation ; 44.984 $PO_4-P$ in 24 hrs. irradiation ; 24.237 $(NH_3-N)+(NO_3-N)$ in 12 hrs. irradiation ; 1432.851 $PO_4-P$ in 12 hrs. irradiation ; 599.076

• BMB Reports
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• v.36 no.2
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• pp.159-166
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• 2003

Cytoplasmic $\alpha$-glycerol-3-phosphate dehydrogenase from fruit-bat-breast muscle was purified by ion-exchange and affinity chromatography. The specific activity of the purified enzyme was approximately 120 units/mg of protein. The apparent molecular weight of the native enzyme, as determined by gel filtration on Sephadex G-100 was $59,500{\pm}650$ daltons; its subunit size was estimated to be $35,700{\pm}140$ by SDS-polyacrylamide gel electrophoresis. The true Michaelis-Menten constants for all substrates at pH 7.5 were $3.9{\pm}0.7\;mM$, $0.65{\pm}0.05\;mM$, $0.26{\pm}0.06\;mM$, and $0.005{\pm}0.0004\;mM$ for L-glycerol-3-phosphate, $NAD^+$, DHAP, and NADH, respectively. The true Michaelis-Menten constants at pH 10.0 were $2.30{\pm}0.21\;mM$ and $0.20{\pm}0.01\;mM$ for L-glycerol-3-phosphate and $NAD^+$, respectively. The turnover number, $k_{cat}$, of the forward reaction was $1.9{\pm}0.2{\times}10^4\;s^{-1}$. The treatment of the enzyme with 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) under denaturing conditions indicated that there were a total of eight cysteine residues, while only two of these residues were reactive towards DTNB in the native enzyme. The overall results of the in vitro experiments suggest that $\alpha$-glycerol-3-phosphate dehydrogenase of the fruit bat preferentially catalyses the reduction of dihydroxyacetone phosphate to glycerol-3-phosphate.

• Journal of the Korean Chemical Society
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• v.33 no.6
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• pp.662-668
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• 1989

The decomposition reaction of hydrogen peroxide was carried out by using metal-4,4',4",4"'-tetraaminophthalocyanine [Mt-$PcNH_2$, Mt = Fe(III), Co(II)] supported on poly (styrene-co-methacrylic acid), in heterogeneous aqueous system. These catalysts showed a catalse-like activity and Fe(III)-$PcNH_2$ supported on the copolymer was particularly effective for the decomposition of hydrogen peroxide. It was found that the rate of decomposition increased smoothly in the higher pH region and catalytic reaction was interfered by adding $CN^-,\;CNS^-,\;{C_2O_4}^{-2},\;I^-$ ions. The kinetics of the catalytic reaction was also investigated and the reaction proceeds according to the Michaelis-Menten type mechanism.