• Molecules and Cells
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• v.40 no.10
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• pp.761-772
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• 2017

Glioblastoma is the most frequent and most aggressive brain tumor in adults. Solute carrier family 8 member 2 (SLC8A2) is only expressed in normal brain, but not present in other human normal tissues or in gliomas. Therefore, we hypothesized that SLC8A2 might be a glioma tumor suppressor gene and detected the role of SLC8A2 in glioblastoma and explored the underlying molecular mechanism. The glioblastoma U87MG cells stably transfected with the lentivirus plasmid containg SLC8A2 (U87MG-SLC8A2) and negative control (U87MG-NC) were constructed. In the present study, we found that the tumorigenicity of U87MG in nude mice was totally inhibited by SLC8A2. Overexpression of SLC8A2 had no effect on cell proliferation or cell cycle, but impaired the invasion and migration of U87MG cells, most likely through inactivating the extracellular signal-related kinases (ERK)1/2 signaling pathway, inhibiting the nuclear translocation and DNA binding activity of nuclear factor kappa B ($NF-{\kappa}B$), reducing the level of matrix metalloproteinases (MMPs) and urokinase-type plasminogen activator (uPA)-its receptor (uPAR) system (ERK1/2-$NF-{\kappa}B$-MMPs/uPA-uPAR), and altering the protein levels of epithelial to mesenchymal transitions (EMT)-associated proteins E-cardherin, vimentin and Snail. In addition, SLC8A2 inhibited the angiogenesis of U87MG cells, probably through combined inhibition of endothelium-dependent and endothelium-nondependent angiogenesis (vascular mimicry pattern). Totally, SLC8A2 serves as a tumor suppressor gene and inhibits invasion, angiogenesis and growth of glioblastoma.

• Korean journal of food and cookery science
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• v.11 no.5
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• pp.472-478
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• 1995

녹두가루와 전분의 일반성분 및 무기성분을 조사하고 녹두가루의 농도(4-8%,건량기준), 녹두전분의 농도(3-8%,건량기준)에 따른 호화 특성 및 리올로지특성을 비교하였다. 녹두가루의 일반성분은 수분 6.8%, 단백질 25.0%,지방 1.21%,회분 1.77%,비타민 B$_1$과 B$_2$는 각각 0.57과 0.11 mg/100 g이었다. 전분의 비타민 함량은 B$_1$이 0.002 mg/100 g, B$_2$가 0.02 mg/100 g이었다. 가루의 무기질함량은 칼슘이 374.9 mg/100 g으로 가장 높았고 인(353.0 mg/100 g), 칼륨(176.3 mg/100 g), 마그네슘(116.9 mg/100 g), 나트륨(107.6 mg/100 g) 순서이었다. 전분현탁액의 광투과도로 부터 예측한 전분의 호화온도는 67$^{\circ}C$이었다 녹두가루와전분의 팽윤력은 60-8$0^{\circ}C$의 가열온도에서 직선적으로 증가하였으며 그 정도는 전분이 가루보다 컸다. 용해도는 $65^{\circ}C$이후부터 증가하여 녹두 가루는 8$0^{\circ}C$까지 지속적으로 증가하였으나 전분은 7$0^{\circ}C$ 이후에는 완만하게 증가하였다 녹두가루는 농도가 증가함에 따라 아밀로그래프의 최고 점도가 증가하였으나 전분은 6-8%의 농도에서 최고점도를 나타내지 않았으며92.5$^{\circ}C$에서 15분간 유지하는 동안 점도는 지속적으로 증가하였다. 최고점도의 대수값과 농도는 직선적인 관계를 나타냈으며 동일한 최고점도를 나타내는 농도는 전분이 가루보다 2.6ft'3도 낮았다. 녹두가루(4-8%, 건량기준)와 전분(3-8%, 건량기준)현탁액을 95$^{\circ}C$에서 40분간 유지시켜 호화시킨 액을 6$0^{\circ}C$에서 측정한 결과, 항복응력과 점조 도지수는 농도가 증가함에 따라 커졌고 유동지수값은 1.0보다 작아 녹두가 루와 전분호화액은 항복응력을 가진 의가소성 유체의 성질을 나타내었다 동일한 점조도지수값을 나타내는 농도는 전분이 가루보다 약 1.3%정도 낮았다.

• Journal of Preventive Medicine and Public Health
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• v.40 no.1
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• pp.71-76
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• 2007

Objectives : This study was performed to investigate the distribution of apolipoproteins A-I and B among Korean employees and their partners. Methods : The study population consisted of 7,633 men and women (4,578 men and 3,054 women) residing in Seoul and Kyung-gee Do, with an average age of $43.5{\pm}8.3$ years. Blood samples were collected following at least 12 hours of fasting. Apolipoproteins A-I and B were measured using a Behring Nephelometer analyzer. The body mass index (BMI) for each participant was calculated as weight (kg) divided by height squared $(m^2)$. Information on health-related behaviors such as exercise, alcohol intake, and smoking habits was collected through self-administrated questionnaires. Results : The mean concentrations of Apo A-I were $132.6{\pm}22.3mg/dL$ and $142.9{\pm}24.8mg/dL$ in the men and women, respectively. The concentration of Apo A-I increased significantly across all age categories of men. The mean concentrations of Apo B were $101.7{\pm}23.2mg/dL$ and $87.8{\pm}23.5mg/dL$ in the men and women, respectively, and Apo B increased significantly across all age categories for both the men and women. Exercise and BMI were major determinants for Apo A-I and B levels. The 10th percentile of Apo A-I concentration was 109 mg/dL in the men and 113 mg/dL in the women, and the 90th percentile of Apo B concentration was 131 mg/dL in the men and 118 mg/dL women. Conclusions : For the prevention of coronary artery disease, we recommend that for individuals in the 10th percentile of concentration for Apo A-I and the 90th percentile of concentration for Apo B, active preventive interventions such as weight loss and exercise should be taken. This study, within its limitations, may be useful for evaluating apolipoprotein A-I and B concentrations in Korean adults.

• Korean Journal of Pharmacognosy
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• v.36 no.2 s.141
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• pp.70-74
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• 2005

High performance liquid chromatography(HPLC) was used for the determination of eleutherosides B and E in various parts of Acanthopanax species. Eleutheroside B was detected in the stem (1.45 mg/g) and the root (0.59 mg/g) of A. senticosus and the stem (0.31 mg/g) of A. senticosus grafted on A. sessiliflorus. But there is no detection of eleutheroside B in A. sessiliflorus and the root of A. senticosus grafted on A. sessiliflorus. Eleutheroside E was detected in the stem (0.92, 0.97 and 0.30 mg/g, respectively) and the root (0.65, 0.94, 0.34 mg/g, respectively) of A. senticosus, A. sessiliflorus and A. senticosus grafted on A. sessiliflorus.

• Journal of Korean Society of Water and Wastewater
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• v.24 no.2
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• pp.195-202
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• 2010

The evaluation of organic matters and nutrients removal was investigated for the synthetic wastewater and the high strength of dairy wastewater. Two different systems were performed for this research. System A composing of a single RBC with tapered aeration was fed with the synthetic wastewater for 74 days with 173L/day of influent, 200% of internal return and 100% of sludge return for the period 1 and 2. The feed conditions were maintained 346L/day of influent, 50% of internal return and sludge return for the period 3. The dairy wastewater was introduced to evaluate treatment efficiency for system B composing of dual RBCs and tapered aeration tanks for 50 days of experimental run time, in which hydraulic rates were maintained at the constant ratios of 346L/day, 50% of internal return and 50% of sludge return. The spiral string media made of nylon fibre was attached by Bacillus sp. in RBC for both systems. The specific area of string media was $1.4m^2$/m and biomass was maintained at the concentrations of 23g/m. The synthetic wastewater was supplied by 1,800mg/L of glucose, 500mg/L of $NH_4Cl$, and 500mg/L of $KH_2PO_4$ to system A. The dairy wastewater was supplied to system B from dairy production plant. The average influent concentrations were 1,334mg/L of BOD, 2,014mg/L of CODcr, 160mg/L of T-N, and 12mg/L of T-P in system A. The average influent concentrations of parameters were 1,006 mg/L for BOD, 1,875mg/L for $COD_{cr}$, 51.6mg/L for T-N and 8.9mg/L for T-P in system B. Results indicated that removal efficiencies of BOD and $COD_{cr}$ were more than 90% however, the removal efficiency of T-N was 87%, and that of T-P was 82% for system A. Removal efficiencies were 98.5% of BOD, 91.3% of nitrogen and 89% of phosphorus for system B. The removal efficiencies of organic matters, T-N and T-P were higher in system B than in system A. The effluent quality issued by the stringent national legislations for the discharge of the high strength of dairy products wastewater can be improved using sequential RBCsand tapered aeration reactors rather than a single RBC and tapered aeration reactors with Bacillus sp.

• Korean Journal of Materials Research
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• v.9 no.1
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• pp.8-13
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• 1999

$ZrB _2$ was prepared from a mixture of $ZrO_2$, $B_2$$O_3$and Mg by SHS method. The combustion products were successfully obtained from a mixture of $Zro_2$:$B_2$$O_3$:Mg=1:2.0:8.5(molar ratio). MgO, by-product, was removed to 92.7% by leaching with 1M HCl solution at 9$0^{\circ}C$, for 10 hours. After leaching, the mean particle size of the resultant $ZrB_2$powders was 23.6$\mu\textrm{m}$. $ZrB_2$-ZrC composite was suitably obtained from a mixture of C/Zr=1.2 molar ratio by arc-melting method. The density of arc-melted specimen increased by adding excess zirconium content(x). The bulk density was 6.17g/㎤ for x=0, and 6.37g/㎤ x=4. Vickers hardness of arc-melted specimen was /$1290kg\textrm{mm}^2$ for x=0, and fracture toughness increased to 4.2MPa.m\ulcornerforx=4 compared to 3.4MPa.m\ulcornerfor x=0.

• Culinary science and hospitality research
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• v.17 no.4
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• pp.251-262
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• 2011

The preference for maesengee sauce was investigated by developing 3 types of maesengee sauces. Through the comparative analysis of the developed maesangee sauces, the study aimed to develop maesangee sauce as a health functional food and to activate the local economy by the development of maesangee sauce as the processed food in the future. For the composition analysis of maesangee, the maesangee that was purchased from Gangjin-gun in July, 2009 was used as a base material. Based on the investigation results of preference for maesangee sauces, the following 3 types of maesangee sauces including maesangee red vinegar sauce, maesangee plain yogurt sauce and maesangee wrapping sauce were developed. By performing the comparative analysis of preference for the developed maesangee sauce types, this study aimed to put perfection in the research. The sample product of the developed maesangee sauce was used for the questionnaire survey for 42 students attending culinary colleges located in Gwangju from November 9, 2009 to November 16, 2009. For the statistical analysis, frequency analysis was used. The composition analysis result of maesangee revealed the presence of p(227.98mg/100g), Ca(58.32mg/100g) and Fe(7.8mg/100g), No detection of Vitamin A($0{\mu}gRE$/100g), Vitamin $B_1$(0.0837mg/100g), Vitamin $B_2$(0.0641mg/100g), Vitamin B6(0.0261mg/100g), Vitamin C(19.9877mg/100g), Vitamin E(0.3677mgaTF/100g), Vitamin K($7.2079{\mu}g$/100g), and Niacin(1.5057mgaNF/100g). Based upon the questionnaire survey results, the sample product development of maesangee red vinegar sauce, maesangee plain yogurt sauce and maesangee wrapping sauce was achieved along with their preparation methods.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.6
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• pp.784-793
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• 2004

Major objective was to evaluate three doses of bST (POSILAC(R)) injected into Holstein cows during the transition period and through 56 d of lactation for potential to improve DMI, BCS, BW, metabolites, hormones, IGF-I and milk production. Biweekly injections of bST (0, 5.1, 10.2, or 15.3 mg bST/d) began 28 d before expected parturition and continued through 56 d postpartum. Twenty-three of the 25 multiparous Holstein cows assigned randomly to four groups completed experiment (7, 5, 6 and 5 cows/group, respectively). The DMI, BW and BCS were recorded weekly throughout the prepartum and postpartum periods and blood samples were collected thrice weekly for analyses of ST, insulin, $T_{4}$, $T_{3}$, IGF-I, glucose and NEFA. Milk yields were recorded daily through 60 d postpartum and milk components measured once weekly. Mathematical model for data analyses for prepartum and postpartum periods included treatment, calving month, and the two-factor interaction. Cows injected with 10.2 and 15.3 mg bST prepartum had greater mean prepartum concentrations of ST and IGF-I. Prepartum injections of bST did not affect prepartum BW or BCS. On average, cows injected postpartum better maintained their BCS during first 60 d of lactation (3.15$\pm$0.06, 3.12$\pm$0.007, 3.20$\pm$0.006 and 3.58$\pm$0.009). Treatments did not affect mean prepartum DMI but cows injected with 15.3 mg bST/d had greatest DMI and greatest mean daily MY during the first 3 wk and tended to be greater during first 60 d of lactation. Cows injected with two highest bST doses (10.1 and 15.2 mg/d) had greater mean postpartum concentrations of ST and $T_{3}$, but IGF-I, $T_{4}$, glucose and NEFA did not differ across groups. No adverse effects of bST treatment were observed.

• Korean Journal of Food Science and Technology
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• v.12 no.1
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• pp.77-81
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• 1980

The effect of dietary vegetable oils, sesame, perilla, soybean and rice bran oils, on the blood cholesterol level of rabbit was examined using isocaloric and isonitrogenous diets. The gain in body weight, liver weight, serum and liver cholesterol levels, globulin, blood sugar and acid phosphatase activity in relation to cholesterol level were studied. The results are summarized as follows : 1. The gain in body weight (g/day) of rabbit was 16.3 for control, 15.3 for A, 18.3 for 15.3 for A, 18.3 for B, 15.3 for C and 18.1 for D groups. 2. Liver weight of the control A, B, C and D groups were 30.35, 37.25, 38.25, 31.05 and 39.54, respectively. 3. Serum cholesterol levels (mg/100 ml serum) of the control, A, B, C, and D groups were 71.5, 112.0, 110, 93 and 96 respectively. 4. Liver cholesterol levels (mg/100 ml liver fat) of the control, A, B, C and D groups were 255, 292, 255, 317 and 195 respectively. 5. The contents of alpha-1-globulin for control was 0.60 %, for C, 0.35 % and for D groups, 0.32% of total globulin. The content of alpha-2-globulin for control was 0.68 % of total globulin and for D, 0.26 % of total globulin. 6. The contents of blood glucose (mg/100 ml) of the control, A, B, C and D groups were 40.34, 22.37, 77.0, 28.6 and 34.1 respectively. 7. Acid phosphatase of the control, A, B, C, and D groups were 3.95, 4.52, 3.98, 4.55 and 4.53 nM/hr/l serum respectively. 8. According to the regression and correlation in coefficient in blood components of rabbit, there were positive correlations between serum cholesterol and alpha-1-globulin, and between liver cholesterol and gamma-globulin.

• The Korean Journal of Food And Nutrition
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• v.11 no.3
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• pp.319-322
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• 1998

The whole cell of alkalophilic Streptomyces sp. B-2 which produce glucose isomerase was immobilized by entrapment method for the effective production of high fructose syrup. The highest immobilized activity was achieved when the enzyme was bound to 2% $textsc{k}$-carrageenan. Immobilized glucose isomerase the pH optimum was about pH 7.5~8.5. Immobilization of alkalophilic Streptomyces sp. B-2 on 2% $textsc{k}$-carrageenan at 7$0^{\circ}C$ showed an increase in glucose isomerase activity. GI activity of immobilized cells was maximum Co2+ concentration 10-3M, Mg2+ concentration 10-3M.