• YAKHAK HOEJI
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• v.37 no.3
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• pp.278-285
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• 1993

The anticlastogenic effect of N-acetylcysteine was tested in vivo in mouse bone-marrow micronucleus assay. The frequencies of micronuclei induced by adriamycin (5 mg/kg i.p.) in bonemarrow cells were decreased by the oral administration of N-acetylcysteine at 12 h before adriamycin injection. The observed suppressing effect was not a reflection of a delay in the formation of micronuclei by the cytotoxic effect of N-acetylcysteine. The anticlastogenic effects of SH compound including N-acetylcysteine, cysteine, cystine, S-carboxy methylcysteine and glutathione were also investigated by the multiple pretreatment. Each SH compound was administered orally every day for 5 days and adriamycin (5 mg/kg i.p.) was injected at 24h after the last dose of test compound. N-acetylcysteine and glutathione showed significantly the suppressive effect at dose of 10 and 25 mg/kg for N-acetylcysteine and at the dose of 25 mg/kg for glutathione. Our study suggests that N-acetylcysteine is capable of protecting the chromosomal damages in the normal cells during cancer chemotherapy by adriamycin, and may act as an anticlastogen against induction of micronuclei by superoxide generating agent such as adriamycin.

• Archives of Pharmacal Research
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• v.20 no.1
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• pp.53-57
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• 1997

For the purpose of defining the effects of the N-substituted alkyl groups on the anticonvulsant activities of N-Cbz-.alpha.-aminosuccinimides, various (R)- and (S)-N-alkyl substituted N-Cbz-.alpha.-aminosuccinimides (1 and 2) were prepared from the corresponding (R)- and (S)-N-Cbz-aspartic acid by using known reaction and were evaluated the anticonvulsant activies in the MES and PTZ tests, including their neurotoxicities. The most active compound in the MES test was (R)N-Cbz-.alpha.-amino-N-methylsuccinimide (1b) $(ED_{50}=52.5 mg/kg, Pl=3.2)$. And in case of the PTZ test, (R)-N-Cbz-.alpha.-amino-N-ethylsuccinimide (1c) was the most active compound $(ED_{50}/=32.5mg/kg, Pl=3.1)$. The order of anticonvulsant activities of these compounds against the MES test, as judged from the ED_50values for the R series (1), was N-methyl > N-isobutyl > non-substituted > N-ethyl, N-allyl > N-benzyl compound; for the S series (2) N-methyl > N-altyl > non-substituted > N-isobutyl > N-ethyl > N-benzyl compound. The anticonvulsant activities in the PTZ tests of these compounds exhibited somewhat different pattern ; for the R series (1) Nethyl > N-methyl > N-isobutyl> non-substituted > N-allyl > N-benzyl compound in order of decreasing activity; for S series (2) N-ethyl > N-allyl, non-substituted > N-isobutyl > N-methyl > N-benzyl compound in order of decreasing activity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.4
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• pp.324-330
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• 1994

The objective of this study is to obtain the basic data for investigating the potentiality of continuous utili~ation (first cutting in the late fall and regrowth yield in next spring) of forage rape seeded in fall. Fresh yield and the content of nitrogenous compound in leaves and roots were measured during a growth period. Fresh yield accumulation was very low between the late fall(470kg/10a) and the early regrowth period( 1.070kg /IOa). Total nitrogen content in leaves until the wintering period was decreased from 4.71% to 3.70%. while that in roots slightly increased during this period. The highest content(4.84%) in roots was observed in the early regrowth period, and then rapidly decreased as growth advanced. Protein-N was the largest pool of nitrogenous compound in leaves and roots through entire growth period, Its content in leaves decreased until the wintering period (Feb. 4), and then increased until the bolting stage(Apr. 10). Protein-N in roots highly accumulated from the late fall(11.1mg/gDM) to the early regrowth period(l6.9mg/gDM), and then decreased until the early tlowering stage. The content of amino acid-N in leaves showed a little change with a range from 5.7 to 8.5mgIgDM during entire growth period, while that in roots rapidly decreased from early regrowth period. The content of $NO_3$-N decreased from 7.0 to 4.3mglgDM in leaves, while increased from 0.9 to 2.3mg/gDM in roots from the late fall to the wintering period. 7he content of $NH_4$-N was lower and less varillble than other nitrogen compound during entire growth period. The results clearly showed that protein-N was the main storage form and highly accumulated in roots of overwintering forage rape.

• Archives of Pharmacal Research
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• v.22 no.5
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• pp.491-495
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• 1999

In order to examine the effects of N-substituted alkyl group on the anticonvulsant activities of N-Cbz-$\alpha$-aminoglutarimides as novel anticonvulsants with broad spectrum, a series of (R) or (S) N-Cbz-$\alpha$-amino-N-alkylglutarimides (1 and 2) were prepared from the corresponding (R) or (S) N-Cbz-glutamic acid and evaluated for the anticonvulsant activities in the maximal electroshock seizure (MES) test and pentylenetetrazol induced seizure(PTZ) test, including the neurotoxicity. The most potent compound in the MES test was (S) N-Cbz-$\alpha$-amino-N-methylglutarimide($ED_{50}$=36.3 mg/kg, PI=1.7). This compound was also most potent in the PTZ test ($ED_{50}$=12.5 mg/kg, PI=5.0). The order of anticonvulsant activities against the MES test as evaluated form $ED_{50}$ values for (R) series was N-methyl > N-H > N-ethyl > N-allyl ; for the (S) series N-methyl > N-H > N-ethyl > N-alkyl > N-isobutyl compound. Against the PTZ tests, the order of anticonvulsant activities showed similar pattern ; for the (R) series, N-methyl > N-H > N-ethyl > N-allyl ; for the (S) series N-methyl > N-H > N-ethyl > N-allyl > N-isobutyl compound. From the above results, N-substituted alkyl groups were though to play an important role for the anticonvulsant activities of N-Cbz-$\alpha$-amino-N-alkylgutarimides.

• Journal of the Korean Ceramic Society
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• v.35 no.3
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• pp.303-309
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• 1998

The fabrication of Al2O3/Al composite by pressureless infiltration was investigated by the change of Mg and Si content in Al alloy infiltration process and infiltration atmosphere. The effect of alloying elements infiltration atmosphere and interfacial reactants between Al alloy matrix and Al2O3 particles were in-vestigated in terms of bendingstrength and harness test,. The fabrication of Al2O3/Al composite by the vestigated in terms of bending strength and hardness test. The fabrication of Al2O3/Al composite by the pressureless infiltration was done in nitrogen atmosphere with Mg in Al alloy. It was successfully fabricated at $700^{\circ}C$ according to Mg contents in Al alloy and infiltration condition. Because Mg in the Al alloy and ni-trogen atmosphere of infiltratio condition produced Mg-N compound(Mg3N2) it decreased the wetting an-gle between molten Al alloy and Al2O3 particles by coating on surface of Al2O3 particles. The fracture strength of Al2O3/Al-Mg composite was 800MPa and Al2O3/Al-Si-Mg composite was 400MPa. Si in Al alloy decreased the interfacial strength between Al alloy matrix and Al2O3 particles.

• Analytical Science and Technology
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• v.6 no.3
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• pp.307-311
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• 1993

Ionophore, which contains 5-membered heterocyclic compound, was prepared. $Mg^{2+}$ was determined by salting-out technique using ionophore as a chelating reagent. After $Mg^{2+}$ was extracted into the acetonitrile layer as a Mg-$(Ionophore)_2$ complex from acetate buffered aqueous solution by salting-out extraction technique, absorbance of complex was recorded by atomic absorption spectrophotometry. Optimum pH was between 2.5 and 5.0 for extraction and 1:2([$Mg^{2+}$]/[ionophore]) complex were formed. The range of detection was 0.24ppm~2.4ppm and $Ca^{2+}$ and EDTA were interfered in the determination of $Mg^{2+}$.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2002.04b
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• pp.42-43
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• 2002

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.1
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• pp.52-56
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• 1992

1. Application of N.P.K compound fertilizer increased N.P.K contents in soils and application of peat and Miwon organic fertilizer showed the trend of increasing N contents in soils also. 2. N, Ca and Mg contents in radish leaves(top) were higher than in radish(bottom) but P content was revered. And application of N.P.K compound fertilizer always incresased N content in radish plants regardless of any other fertilzer addition. 3. Application of N.P.K compound fertilizer increased N.P.K contents in cabbage plants more compare to no application of N.P.K compound fertilizer regardless of other fertilizer application. 4. Plant growth status and yield (fresh weight) of radish and cabbage revealed that every fertilizer application increased plant growth and yield compared to no fertilizer application, but N.P.K compound fertilizer showed higher increment compared to organic matter fertilizer application except Miwon(2 level)treatment. However, organic fertilizer application together with N.P.K compound fertilizer level recommended showed the highest in radish and cabbage yield. 5. Effects of four organic fertilizer on yields(fresh weight) of radish and cabbage were in the order of Miwon organic fertilizer ${\geq}$ Biovin organic fertilizer > Compost ${\leq}$ Peat.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.678-684
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• 2010

In an attempt to improve biomass and bioactive compound production, we cultured adventitious roots of $Eleutherococcus$ $koreanum$ in 250 mL Erlenmeyer flasks using Murashige and Skoog (MS) medium with different concentrations of auxins (IBA, NAA, IAA) and cytokinins (BA, kinetin, TDZ). Root biomass (fresh and dry weight) was enhanced at $5mg{\cdot}L^{-1}$ indole-3-butyric acid (IBA) after 5 weeks of culture. The content of total phenolics and flavonoids was also increased with $5mg{\cdot}L^{-1}$ IBA compared to ${\alpha}$-naphtalene acetic acid (NAA) or indole-3-acetic acid (IAA) treatments. The combination of $5mg{\cdot}L^{-1}$ IBA with $0.1mg{\cdot}L^{-1}$ thidiazuron (TDZ; N-phenyl-N'-1,2,3,-thidiazol-5-ylurea) enhanced root fresh and dry weight (1.4- and 1.6-fold, respectively) as well as the content of total phenolics and flavonoids compared to the relative control (without cytokinin). On the contrary, $N_6$-benzyladenine (BA) and 6-furfurylaminopurine (kinetin) did not significantly affect root biomass and bioactive compound production in adventitious roots of $E.$ $koreanum$. These results suggested that $5mg{\cdot}L^{-1}$ IBA combination with $0.1mg{\cdot}L^{-1}$ TDZ supplementation was most suitable for both biomass and bioactive compound production from adventitious roots of $E.$ $koreanum$.

• Journal of the Korean Ceramic Society
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• v.35 no.7
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• pp.685-690
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• 1998

The wetting behavior and the characteristic of spontaneous infiltration of pure Al and Al-(Si)-Mg alloys on {{{{ { {Al }_{ 2} O}_{3 } }} in vacuum argon and nitrogen atmosphere were investigated to find out the spontaneous in-filtration mechanism. The wetting of molten Al and Al alloys on {{{{ { {Al }_{ 2} O}_{3 } }} was only possible in cacuum at-mosphere but the sponataneous infiltration of molten Al-(Si)-Mg alloys was successfully made on {{{{ { {Al }_{ 2} O}_{3 } }} pre-form in nitrogen atmoshpere. The difference of wettability and spontaneous infiltration of molten Al and Al alloys on {{{{ { {Al }_{ 2} O}_{3 } }} were found to be related to formation of the Mg-N compound coated layer on {{{{ { {Al }_{ 2} O}_{3 } }} particles which was believd to increase wettability of molten Al alloys on {{{{ { {Al }_{ 2} O}_{3 }.