• Journal of Microbiology and Biotechnology
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• v.5 no.5
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• pp.264-268
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• 1995

Sulforhodamine B(SRB) assay was performed on the human lung carcinoma, A549 cell line to screen soil microorganisms for production of anti-cancer agent. Among 4, 265 microorganisms, 45 isolates were selected for their cytotoxicity and tested for their effects on the expression of c-myc by RNA slot blot and Northern blot analysis resulting in selection of No.2303 isolate. This No.2303 was identified as Streptomyces sp. by ISP classification and the chemotaxonomic analysis method. NO.2303 inhibited the expression of cmyc in Col0320 DM and A549 cell lines. The culture extract of No. 2303 also inhibited the progression of the cell cycle of Go in NIH 313 cells, implying that the extract also inhibited the expression of c-myc in NIH 313 cell.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.7
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• pp.3145-3149
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• 2014

Gene expression profiling facilitates the understanding of biological characteristics of gliomas. Previous studies mainly used regression/variance analysis without considering various background biological and environmental factors. The aim of this study was to investigate gene expression differences between grade III and IV gliomas through partial least squares (PLS) based analysis. The expression data set was from the Gene Expression Omnibus database. PLS based analysis was performed with the R statistical software. A total of 1,378 differentially expressed genes were identified. Survival analysis identified four pathways, including Prion diseases, colorectal cancer, CAMs, and PI3K-Akt signaling, which may be related with the prognosis of the patients. Network analysis identified two hub genes, ELAVL1 and FN1, which have been reported to be related with glioma previously. Our results provide new understanding of glioma pathogenesis and prognosis with the hope to offer theoretical support for future therapeutic studies.

• Journal of the Korean Society of Costume
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• v.52 no.4
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• pp.155-171
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• 2002

Trompe-1’oeil technique strategically conceived with a view to effectively realize creative ideas among the expression techniques of fine arts style has provided the driving force in development of fine arts and has continuously influenced development of the modern fashion pursuing unique individuality. The purpose of this study is to open a new horizon for the development of fashion as a practical art, and to seek the expansion of the creative domain and ultimately to contribute to the creation of original and creative fashion by examining the interrelationship between Trompe-1’oeil, which has long been utilized and positioned as one of the leading fine arts techniques with the advent of surrealism in the beginning of the 20th century and the modern fashion. The study is focused first on finding out how Trompe-1’oeil technique originated in connection with researching the fashion of Trompe-1’oeil and on analysing the techniques of expression, and then on investigating into Elsa Schiaparelli, pioneer of Trompe-1’oeil technique to identify her influences, and finally on classifying clothes employing Trompe-1’oeil technique by their expression method to examine how Trompe-1’oeil technique has been applied to modern clothes. As for the research method, the researcher has referred to fine arts books, collection of pictorial records and the like to gain conceptual understanding of Trompe-1’oeil and to examine the expression method and the features of Trompe-1’oeil, and collected and referred to fashion books and fashion marazines to understand Elsa Schiaparelli and the expression tendencies of Tromprf-1’oeil in modern fashions. Particularly, the researcher has attempted to search the correlation between modern fashion and Trompe-1’oeil technique. As a result of this research, the researcher has managed to classify Trompe-1’oeil technique expressed in modern fashion into ‘harmony’, ‘application of the human body’, ‘front and back’, ‘surface and inside’. ‘completion of the incomplete’ and ‘detail.’ The researcher has also noted that Elsa Schiaparelli, a surrealist first applied Trompe-1’oeil technique to clothes and confirmed that quite a few avant-garde clothes designers following Elia Schiaparelli, by using Trompe-1’oeil technique in clothes, recently recreate fresh feelings.

• The Korean Journal of Nuclear Medicine
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• v.34 no.1
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• pp.1-9
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• 2000

The rapid progress of molecular genetic methods over the past two decades has necessitated the development of methods to detect and quantify genetic activity within living bodies. Reporter genes provide a rapid and convenient tool to monitor gene expression by yielding a readily measurable phenotype upon expression when introduced into a biological system. Conventional reporter systems, however, are limited in their usefulness for in vivo experiments or human gene therapy because of its invasive nature which requires cell damage before assays can be performed. This offers an unique opportunity for nuclear imaging techniques to develope a novel method for imaging both the location and amount of gene expression noninvasively. Current developments to achieve this goal rely on utilizing either reporter enzymes that accumulate radiolabeled substrates or reporter receptors that bind specific radioligands. This overview includes a brief introduction to the background for such research, a summary of published results, and an outlook for future directions.

• Korean Institute of Interior Design Journal
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• v.18 no.6
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• pp.44-51
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• 2009

Various and complex cultural phenomena have led to many changes in the space. The concept of unconsciousness, among others, and experimental expression technique and the concepts from surrealism that rather attaches great importance to contingency than intentional attempt have been employed in the space. The abstract expression as expressional characteristics of surrealism makes the space ambiguous through the concept of automatism using the approach of indeterminism and double image expression, and represents the space providing diversity through the dualism. In contrast, objective expression, which was based on depaysement, generates the tension and expresses the dramatic situation in such a way and manner which are intentional, distorted transformed, together with heterogenous meanings aligned through the reversal appeared in the techniques of collage and paranoiac-critical method. However if such space accommodating the expressional characteristics of surrealism is approached as superficial concept in analyzing the human's inner consciousness and imaginary society, expression of the ultimate concept contained in the expressional characteristics of surrealism can hardly be achieved. Hence, the spatial analysis from the semiotic approach, instead of unilateral method aimed to convey the meaning only, which creates the signification, was adopted. The study, as effective approach to the space accommodating the expressional characteristics of surrealism, was intended systematically evaluate the concreteness of understanding and generation of meaning through the semiotic method. Greimas' semiotic square analysis to the space plays the important role in generating the spatial meaning, as well as is needed for objective analysis of the importance of various characteristics and methodology which the space contains through the expressional characteristics of surrealism.

• Women's Health Nursing
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• v.14 no.2
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• pp.143-149
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• 2008

Purpose: This study was done to examine the relationship between self-reported attachment style, trait anger and anger expression in adolescent women. Method: Five hundred and eighty adolescent women were recruited from March to May, 2006. The instruments were Spielberger's state-trait anger expression inventory-Korean version (Chon, Han, Lee, & Spilelberger, 1997), and Batholomew & Holowitz's attachment style Questionnaire (1991). Data was analyzed by descriptive statistics, and ANOVA using the pc-SPSS (version 10.0) program. Result: The mean score of trait anger and anger-in were higher in adolescent women with an insecure attachment style compared to women with a secure attachment style. Conclusion: This finding suggests that family environment factors such as attachment styles are related to trait anger and anger expression in adolescent women. There is a need to further clarify the relationship of attachment style, trait anger and anger expression in adolescent men.

• International Journal of Internet, Broadcasting and Communication
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• v.10 no.4
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• pp.43-49
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• 2018

Modern people communicate in a digital space without being limited by physical space and time. On the internet, people's desire for self-expression is getting bigger, and the most common self-expression tool is characters. Characters are visual materials to express human thoughts and desires. This study is a method to positively express the viewer's self in an interactive Hangeul system. The proposed system produces new formative shapes and meanings of Hangeul through the process of deconstructing and reconstructing the characters. Immediate character transformations that are synchronized with the motion of the viewer act as a tool of self-expression by inducing the viewer's active interaction repeatedly. Therefore, the proposed interactive Hangeul is more than enough to be used as a new graphic tool beyond the text to be read, and it is highly likely to develop into cultural contents using it.

• Journal of Digital Convergence
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• v.12 no.12
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• pp.243-248
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• 2014

In this paper, we propose a method to recognize the facial expressions by selecting face elements and finding its status. The face elements are selected by using image area segmentation method and the facial expression is decided by using the normal distribution of the change rate of the face elements. In order to recognize the proper facial expression, we have built database of facial expressions of 90 people and propose a method to decide one of the four expressions (happy, anger, stress, and sad). The proposed method has been simulated and verified by face element detection rate and facial expressions recognition rate.

• Journal of the Korea Society of Computer and Information
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• v.14 no.11
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• pp.73-79
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• 2009

In this paper, a method is proposed that enhances the performance of the facial expression recognition using template matching of Local Binary Pattern(LBP) histogram. In this method, the face image is segmented into blocks, and the LBP histogram is constructed to be used as the feature of the block. Block dissimilarity is calculated between a block of input image and the corresponding block of the model image. Image dissimilarity is defined as the weighted sum of the block dissimilarities. In conventional methods, the block weights are assigned by intuition. In this paper a new method is proposed that optimizes the weights from training samples. An experiment shows the recognition rate is enhanced by the proposed method.

• Journal of Pharmacopuncture
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• v.6 no.2
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• pp.45-56
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• 2003

Objective : The purpose of this study was to investigate the effects of Bee Venom on the lipopolysaccharide(LPS), sodium nitroprusside(SNP), hydrogen peroxide$(H_2O_2)$-induced expression inducilble nitric oxide synthetase(iNOS), tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) and nuclear factor kappa B(NF-kB) in RAW 264.7 cells, a murine macrophage cell line. Method : The expressions of expression iNOS and TNF-${\alpha}$ were determined by western blotting with corresponding antibodies. The expressions of expression NF-kB was assayed by EMSA method. Results : 1. The 0.5, 1 and $5{\mu}g/mg$ of bee venom on LPS-induced expression of iNOS, the $5{\mu}g/mg$ of bee venom on SNP-induced expression of iNOS and the $1{\mu}g/mg$ of bee venom on $H_2O_2$-induced expression of iNOS compared with control were inhibited significantly. 2. The 0.5, 1 and $5{\mu}g/mg$ of bee venom inhibited significantly LPS and $H_2O_2$-induced expression of TNF-${\alpha}$ compared with control, respectively. The $0.5{\mu}g/mg$ of bee venom increased significantly SNP-induced expression of TNF-${\alpha}$ compared with control. 3. The $5{\mu}g/mg$ of bee venom on LPS-induced expression of NF-kB, the $0.5{\mu}g/mg$ of bee venom on SNP-induced expression of NF-kB and the 0.5, $5{\mu}g/mg$ of bee venom on $H_2O_2$-induced expression of NF-kB were inhibited significantly compared with control, respectively.