• Textile Coloration and Finishing
• /
• v.12 no.5
• /
• pp.315-322
• /
• 2000

The purpose of this study was to investigate the antibacterial effects of natural colors extracted from yellow natural dyes(Tumeric, Amur Cork Tree and Onion Shell). The water and the methanol extracts of Tumeric and Amur Cork Tree significally decreased the growth of E. coli in vitro and the methanol extract of Tumeric exhibited the strongest inhibitory effect among the samples. Silk and nylon fabrics dyed with water and methanol extracts of the yellow natural dyes showed antimicrobial activities against E. coli and Staph aureus in the Bioassay Test. Nylon fabric dyed with methanol extracts of them showed strong antibacterial effect on E. coli compared with that of water extracts. However, slik fabrics dyed with the extracts could not reduce the growth of E. coli. Silk or nylon fabrics dyed with methanol or water extracts of yellow natural dyes showed antimicrobial activities against Staph aureus. The antimicrobial activity of the fabrics dyed with methanol extracts from Tumeric, Amur Cork Tree and Onion Shell was stronger than that of water extracts, especially, the fabrics dyed with Tumeric extract showed the highest antibacterial property among the dyed fabrics.

• Food Science and Biotechnology
• /
• v.14 no.3
• /
• pp.311-316
• /
• 2005

This study was performed to investigate the antimicrobial effect of Sophora angustifolia extracts against food-borne pathogens. First, Sophora angustifolia was extracted with methanol at room temperature, and the methanol extracts from Sophora angustifolia were fractionated using petroleum ether, chloroform, ethyl acetate, and methanol. The antimicrobial activity of the Sophora angustifolia extracts was determined using the paper disc method against food-borne pathogens and food spoilage bacteria. The methanol extracts of Sophora angustifolia showed the highest antimicrobial activity against Staphylococcus aureus and Salmonella typhimurium. A synergistic effect was found in the combined extracts of Sophora angustifolia and Portulaca oleracea, compared to the activity of each extract alone. Finally, the growth inhibition curve was determined using the methanol extracts of Sophora angustifolia against Staphylococcus aureus and Salmonella typhimurium. The methanol extract of Sophora angustifolia showed strong antimicrobial activity against Staphylococcus aureus at a concentration of 5,000 ppm. The 5,000 ppm methanol extract from Sophora angustifolia retarded the growth of S. aureus for more than 24 hours and of Salmonella typhimurium for up to 12 hours.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.34 no.6
• /
• pp.750-754
• /
• 2005

The antioxidative capacity of methanol extracts prepared from jujubes (Zizyphus jujuba) were investigated by measuring total phenolic compounds, electron donating ability and induction period on lard. The extracts of large, medium and small jujubes of $80\%$ methanol extracts (methanol extracts) and soaking in the water before $80\%$ methanol extracts (water-methanol extracts) were Iyophilized. Total phenolic compounds of small, medium and large jujube of methanol extracts were 326.46 mg$\%,223.13\\;mg\%,\;158.06\;mg\%$ and that of water-methanol extracts were $113.23\;mg\%,\;81.45\;mg\%,\;72.78\;mg\%$, respectively. The Electron donating ability for the water-methanol extracts added samples of large (1.5$\~$3.0$\%$), medium and small jujube $(1.0\~3.0\%)$ were superior to the $0.02\%$ BHT added sample. On the induction period, all samples with jujube extracts were exhibited higher Antioxidant Index (AI: Induction period of lard with jujube extracts divided by induction period of lard) than that of the control and the water-methanol extracts were more effective than the methanol extracts on the antioxidative capacity for the lard. This result indicated that two lyophilized extracts of jujubes showed excellent free radical scavenging ability and long induction period on lard.

• Preventive Nutrition and Food Science
• /
• v.14 no.3
• /
• pp.195-200
• /
• 2009

This study was carried out to investigate the biological activities of star anise extracts obtained from four different solvents (water, methanol, ethanol and chloroform) by measuring total polyphenol and flavonoid contents, DPPH radical scavenging, hydroxyl radical scavenging, nitrite scavenging activity and antimicrobial activity. The methanol extract showed the highest extraction yield, followed by ethanol, water and chloroform. The properties of the extracting solvents significantly affected the total polyphenol content. Methanol extracts contained more total polyphenols than any other extracts. The highest DPPH radical scavenging activity was found in methanol extract. The hydroxyl radical and nitrite scavenging activity were highest in methanol and ethanol extracts. In antimicrobial activity, water extract showed stronger activities than methanol and ethanol extract against Micrococcus luteus and Bacillus subtilis, and no inhibitory effects on Gram negative bacteria were found in all extracts at the concentration used.

• Mycobiology
• /
• v.37 no.3
• /
• pp.203-206
• /
• 2009

To develop new antidementia nutraceuticals, a potent acetylcholinesterase (AChE)-inhibiting extract was screened from various extracts of nutritional mushrooms and lichens nutritional and its physicochemical properties were investigated. Among the several extracts tested, methanol extracts of Umbilicaria esculenta fruiting body showed the highest AChE inhibitory activity of 22.4%. U. esculenta AChE inhibitor was maximally extracted when fruiting bodies were treated with 80% methanol at $40^{\circ}C$ for 18 h. The methanol extracts contained 18.9% crude lipid, 18.8% crude protein, and 11.6% total sugar. In addition, they contained 444 mg/g glutamic acid, 44 mg/g histidine, and 41 mg/g aspartic acid. The methanol extracts were soluble in a solution of methanol and 20% dimethylsulfoxide, insoluble in n-hexane, chloroform, and water, and were stable at $20{\sim}60^{\circ}C$ and pH $1.0{\sim}5.0$ for 1 h.

• Culinary science and hospitality research
• /
• v.9 no.4
• /
• pp.69-80
• /
• 2003

To develope natural food preservatives antimicrobial effect of the natural products against food-related bacteria and yeast The purpose of this study was evaluate antimicrobial effect of the citrus seeds. antimicrobial activities of methanol extracts from the citrus seeds investigate against Escherichia coli O26, Staphylococcus aureus 6358, Saccharomyces cerevisiae IBM 4274, Bacillus licheuiformis 9945a and Alcoligenes faecalis. Citrus seeds is containing to moisture 4∼6.5%, curd protein 11∼15%, curd lipid 32∼46%, curd carbohydrate 22∼45 % and ash 2∼4 %, that is containing to flavornoid 12∼48mg% and phenolic compound 22∼53mg%. Solidity content of the methanol extract from the citrus seeds was 0.8∼1.2%. Almost all of the methanol extracts from citrus seeds exhibited growth inhibiting activities for most of microorganisms tested. The methanol extracts from Citrus grandis, C. sunki, C. sulcata showed the growth inhibitory effects against Escherichia coli O26. The methanol extracts from C. obovoidea, C. sulcata, C. aurantium showed the growth inhibitory effects against Staphylococcus aureus 6358. The methanol extracts from C. obovoidea, C. sulcata, C. tangerina showed the growth inhibitory effects against Saccharomyces cerevisiae IBM 4274. The methanol extracts from C. obovoidea, C sunki, C. sulcata, C. tangerinan, C. natsudaidai, C. iyo, C. aurantium showed the growth inhibitory effects against Bacillus licheuiformis 9945a. The methanol extracts from C. obovoidea, C sunki, C. sulcata, C. aurantium showed the growth inhibitory effects against Alcoligenes faecalis. Among this especially, Showed growth inhibiting activity of the methanol extracts from Citrus sulcata that about microorganisms investigated. If apply searching suitable application method about such the citrus seeds antimicrobial activity, role as good antimicorbial material in storage or cooking of food, processing is expected.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.51 no.4
• /
• pp.322-328
• /
• 2006

A series of methanol extracts from leaf and root parts in spring- and winter-barley plants were assayed to determine their allelopathy and antioxidant activity. The methanol extracts applied on filter paper in a Petri-dish significantly inhibited root growth of Chinese milkvetch (Astragalus sinicus L.) seedlings. Leaf extracts at 25 and 50 g $L^{-1}$ inhibited root growth of Chinese milkvetch seedlings more than root extracts. No difference in phytotoxic effects of spring- and winter-barley seedlings extracts on root growth of Chinese milkvetch was observed. Methanol extracts dose-dependently increased DPPH free radical scavenging activity in vitro. DPPH free radical scavenging activity was higher in the methanol extracts from winter-barley seedlings than in those from spring-barley seedlings, and from leaf extracts than from root extracts. The antioxidant potential of the individual fraction from the methanol extracts of spring-barley seedlings was in order of n-butanol>ethyl acetate>water>chloroform>n-hexane fraction. By means of HPLC analysis, spring-barley (200.62 mg $100g^{-1}$) had more amount of total phenol acid than winter-barley (114.08 mg $100g^{-1}$). Especially, ferulic acid was detected in spring-barley extract (183.46 mg $100g^{-1}$) as the greatest amount. These results suggest that early seedlings of barley plants had potent allelopathy and antioxidant activity, and their activities were differently exhibited depending on plant parts and growing condition.

• Journal of the East Asian Society of Dietary Life
• /
• v.15 no.2
• /
• pp.213-218
• /
• 2005

This study was performed to investigate the antimicrobial effect of Ulmus davidiana var. japonica extracts against food-borne pathogens. First, Ulmus davidiana var. japonica was extracted with methanol at room temperature and the fractionation of the methanol extracts was carried out by using petroleum ether, chloroform, and ethyl acetate, and methanol respectively. The antimicrobial activity of Ulmus davidiana var. japonica extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The methanol extracts of Ulmus davidiana var. japonica showed the highest antimicrobial activity against Staphylococcus epidermidis and Escherichia coli. Finally, the growth inhibition curves were determined by using methanol extracts of Ulmus davidiana var. japonica against Staphylococcus epidermidis and Salmonella typhimurium. The methanol extract of Ulmus davidiana var. japonica had strong antimicrobial activity against Staphylococcus epidermidis at the concentration of 4,000 ppm. At this concentration, the growth of Salmonella typhimurium was retarded more than 48 hours and up to 12 hours for Staphylococcus epidermidis. In conclusion, the methanol extracts of Ulmus davidiana var. japonica inhibit efficiently Staphylococcus epidermidis and Salmonella typhimurium.

• Korean Journal of Plant Resources
• /
• v.21 no.6
• /
• pp.413-419
• /
• 2008

Phenolics level, total flavonoid content, and antioxidant were determined from the methanol extracts of the eight medicinal plants using roots. Total phenolics were found as the highest levels in the methanol extracts of Arctium lappa, and followed by Youngia sonchifolia and Cirsium japonicum. Total amount of the each phenol compounds were detected in C. japonicum extracts ($319.2\;mg\;kg^{-1}$) as the greatest component, and followed by A. lappa ($96.3\;mg\;kg^{-1}$) and Y. sonchifolia ($22.9\;mg\;kg^{-1}$). Total flavonoid content showed the highest amount in methanol extracts from A. lappa (68.1 mg $100\;g^{-1}$) and followed by Y. sonchifolia (11.2 mg $100\;g^{-1}$) Lycoris radiate extracts (87.2%) had the highest nitrite scavenging activity and followed by A. lappa (81.5%) and Y. sonchifolia (77.5%). Methanol extracts of A. lappa at 25 mg $100\;g^{-1}$ exhibited the highest DPPH radical scavenging activity by 90.8%, even though less activity than synthetic antioxidants Vitamin C or butylated hydroxytoluene (BHT). Level of polyphenols was highly correlated with anti oxidative activity ($r^2$ = 0.85). The results suggest that several medicinal plants selected had the potent biological activities, and that their activities were differential depending on plant species.

• YAKHAK HOEJI
• /
• v.40 no.5
• /
• pp.582-590
• /
• 1996

For the research of antifungal active constituents from natural products, 226 plants were extracted with ether and methanol, separately, and screened antifungal activity against Candida albicans and Penicillium avellaneum cells. The results demonstrated that 30 samlpes showed antifungal activity in ether or methanol extracts and 17 samples in ether extracts and 20 samples in methanol extracts against C. albicans. Against P. avellaneum, 19 samlpes showed antifungal activity in ether or methanol extracts and 17 samples in ether extracts and 11 samples in methanol extracts, respectively. The antifungal activity of natural products against C. albicans were showed more sensitive than P. avellaneum, and the polarity of the solvent was not specific in antifungal activity.