• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.610-610
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• 2003

• KSBB Journal
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• 제22권1호
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• pp.62-65
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• 2007

대사 공학을 이용한 생산 균주 개발의 핵심 기술은 원하는 대사산물을 과량으로 얻기 위하여 기존의 대사회로에서 제거, 증폭, 변경을 시켜야 할 유전자를 선정하는 것이다. 대사조절 분석 기법은 대사 흐름이 특정 효소의 활성에 따라 어떻게 변하는지를 예측하는 기술이다. 본 논문에서는 대장균의 threonine 생합성 효소 반응 kinetic model과 대사조절 분석 기법을 이용하여 threonine 생합성 flux를 가장 효과적으로 증가시키기 위하여 활성 증가가 필요한 효소가 aspartate semialdehyde dehyogenase라는 것을 밝혔다. 이러한 결과를 확인하기 위하여 asd가 과발현된 vector와 threonine 생합성 경로의 다른 효소인 aspartate kinase를 coding하는 thrA를 과발현 시키는 vector를 제작하여 threonine 생산 균주인 TF5015에 형질전환하여 threonine 농도를 측정하였다. Flask 배양결과 대사조절 분석 기법으로 확인된 유전자 asd를 과발현시킬 경우가 생합성 경로의 다른 유전자를 과발현시킨 경우보다 더 높은 threonine 농도의 증가를 보였다. 이러한 연구 결과들은 효소 반응 kinetic model과 대사조절 분석 기법을 이용하여 원하는 product를 효율적으로 생산할 수 있는 생산 균주를 제작할 수 있게 할 것이다.

• Journal of Microbiology and Biotechnology
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• 제22권5호
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• pp.659-667
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• 2012

2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.12-12
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• 2005

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.564-564
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• 2003

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.58-58
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• 2002

• Journal of Microbiology and Biotechnology
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• 제28권5호
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• pp.784-795
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• 2018

Bacillus amyloliquefaciens Pc3 was isolated from Antarctic seawater with antifungal activity. In order to investigate the metabolic regulation mechanism in the biosynthesis of lipopeptides in B. amyloliquefaciens Pc3, GC/MS-based metabolomics was used when exogenous indole was added. The intracellular metabolite profiles showed decreased asparagine, aspartic acid, glutamine, glutamic acid, threonine, valine, isoleucine, hexadecanoic acid, and octadecanoic acid in the indole-treated groups, which were involved in the biosynthesis of lipopeptides. B. amyloliquefaciens Pc3 exhibited a growth promotion, bacterial total protein increase, and lipopeptide biosynthesis inhibition upon the addition of indole. Besides this, real-time PCR analysis further revealed that the transcription of lipopeptide biosynthesis genes ituD, fenA, and srfA-A were downregulated by indole with 22.4-, 21.98-, and 26.0-fold, respectively. It therefore was speculated that as the metabolic flux of most of the amino acids and fatty acids were transferred to the synthesis of proteins and biomass, lipopeptide biosynthesis was weakened owing to the lack of precursor amino acids and fatty acids.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.793-794
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• 2005

• BMB Reports
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• 제47권3호
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• pp.158-166
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• 2014

Cell proliferation is a delicately regulated process that couples growth signals and metabolic demands to produce daughter cells. Interestingly, the proliferation of tumor cells immensely depends on glycolysis, the Warburg effect, to ensure a sufficient amount of metabolic flux and bioenergetics for macromolecule synthesis and cell division. This unique metabolic derangement would provide an opportunity for developing cancer therapeutic strategy, particularly when other diverse anti-cancer treatments have been proved ineffective in achieving durable response, largely due to the emergence of resistance. Recent advances in deeper understanding of cancer metabolism usher in new horizons of the next generation strategy for cancer therapy. Here, we discuss the focused review of cancer energy metabolism, and the therapeutic exploitation of glycolysis and OXPHOS as a novel anti-cancer strategy, with particular emphasis on the promise of this approach, among other cancer metabolism targeted therapies that reveal unexpected complexity and context-dependent metabolic adaptability, complicating the development of effective strategies.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.423-423
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• 2005