• Natural Product Sciences
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• v.13 no.2
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• pp.164-168
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• 2007

To investigate natural antibiotics from plant essential oils and to evaluate their synergism with current antimicrobial drugs in inhibiting antibiotic-resistant strains of Streptococcus pneumoniae. The minimal inhibitory concentrations (MICs) of eleven plant essential oils and their main components were established for two antibiotic-susceptible and two antibiotic-resistant strains of S. pneumoniae, using broth microdilution tests. Potential synergism with oxacillin, norfloxacin, or erythromycin was evaluated using a checkerboard microtitre assay. Among the tested oils, Mentha piperita oil and its main component, menthol, exhibited the strongest inhibitory activities against all of the tested strains. The activity of antibiotics against antibiotic-resistant strains of S. pneumoniae was enhanced significantly by combination with Mentha piperita oils and its main component, menthol. In conclusion, the combination Mentha piperita essential oil or menthol with antibiotics could be used to reduce the effective dose of antibiotic and to modulate the resistance of S. pneumoniae strains.

• Journal of Life Science
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• v.19 no.9
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• pp.1333-1336
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• 2009

The purpose of this study was to characterize the aromas of rose tea and rosehip tea. Aroma compounds were extracted by simultaneous distillation and extraction method using a Likens and Nickerson's extraction apparatus. The concentrated aroma extracts were analyzed and identified by GC and GC-MS. Thirty-eight compounds, including phenylethyl alcohol, citronellol, menthol, menthone, linalool and geraniol, were isolated and identified in rose tea. Thirty-six compounds, including menthol, $\alpha$-anethole, $\alpha$-terpinolene, menthone, linalool and 6-methyl-5-heptene-2-one, were isolated and identified in rosehip tea. Large amounts of phenyl ethyl alcohol and citronellol were found in rose tea, while large amounts of menthol and $\alpha$-anethole were found in rosehip tea.

• Journal of the Korean Society of Tobacco Science
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• v.1 no.2
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• pp.149-151
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• 1979

• Korean Journal of Clinical Pharmacy
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• v.26 no.3
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• pp.187-194
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• 2016

Background: Alopecia areata (AA) is a very disturbing and expensive disorder in which the exact etiology is not known and it is yet to be treated completely well. Most alopecia patients exhibit some inflammation in the hair follicles regardless of the causes. The clinical symptoms of alopecia present very diversely while the prime symptom is local intermittent fever which are related to inflamed cells. Methods: This study aimed to evaluate how repetitive intermittent fever can damage the normal human dermal fibroblast (NHDF) cells and investigated the cytotoxic and proliferative effects after application of new candidate drugs (ibuprofen, menthol, cetirizine) for alopecia in comparison to minoxidil. Results: This study demonstrated that ibuprofen, menthol, or/and cetirizine can prevent or slow down the damage of NHDF cells from intermittent fever in early alopecia. Aggressive preventative intervention with those drugs before complete destruction of hair follicle by excessive repetitive fever, is a very important step for alopecia therapy and these drugs are recommended as candidate drugs for alopecia in the future. Conclusion: Aggressive preventative intervention with drugs before complete destruction of hair follicles (NHDF cells) by excessive repetitive fever is a very important step for alopecia therapy or progression.

• Journal of Sericultural and Entomological Science
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• v.2
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• pp.33-39
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• 1962

The results for the investigation of the various aromatic plants applied diets on silkworm raising from spring to autumn crops are found as followings. 1. There was no difference between the chemical menthol solution (1.0%) enriched diet and the normal diet for silkworm physiology. Neither injury nor advantage was obtained from the investigation. 2. It was found that there was no danger to use the mulberry leaves as silkworm diet by planting Mentha arvensis L. in the vacansy of mulberry farm, and no worse effect was found by rubbing the leaves of Mentha arvensis L. to the surface of mulberry leaves before feeding to silkworm. 3. For the investigation due to Perilla Ocymoides var application in stead of menthol plant ascribed in Paragraph (2) was obtained the same result. 4. As a conclusion of the study, the plantation of the both aromatical plants with mulberry trees is harmless for silkworm growing even though farmers worry about these to plant together with mulberry tree on mulberry farm.

• Parasites, Hosts and Diseases
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• v.49 no.3
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• pp.255-263
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• 2011

The present work evaluated the effects of alcoholic extracts of salvia (Salvia officinalis), thyme (Thymus vulgaris), and 2 pure compounds (thymol and menthol) on the viability of Echinococcus granulosus protoscolices in vitro. Four different concentrations of each extract (2,500, 1,500, 1,000, and 500 ${\mu}g$/ml) and 3 different concentrations each of thymol and menthol (50, 10, and 1 ${\mu}g$/ml) were used. Concentration of 2500 ${\mu}g$/ml of both extracts showed a significant protoscolicidal activity on the 6th day. Complete loss of viability of protoscolices occurred with 500 ${\mu}g$/ml concentration of both extracts at day 6 and day 7 post-treatment (PT), respectively. Pure compounds, i.e., menthol and thymol, showed potent effects with 50 ${\mu}g$/ml concentration at day 2 and day 5 PT, respectively. These effects were compared with those of albendazole sulfoxide (800 ${\mu}g$/ml), a commonly used treatment drug for hydatidosis. Krebs-Ringer solution and the hydatid cystic fluid at a ratio of 4:1 was a good preservative solution which kept the protoscolices viable for 15 days.

• Journal of the Korean Society of Tobacco Science
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• v.29 no.2
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• pp.98-103
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• 2007

This study compares menthol migration from fillers to filters and mainstream smoke in a type of leaf tobacco and according to the moisture content differences at the range of $11{\sim}15\;%$. The leaf tobacco used in this study consisted of Korea flue cured upper leaves B1O (KFUB1), A40R (KFUA4), lower leaves C1L (KFLC1), CD4L (KFLCD4), burley upper leaves A3T (KBUA3), lower leaves D3W (KBLD3), Orient Basma I/III (OB), Orient Izmir BIG (OI), expanded tobacco (KET), and reconstituted sheet (KRC). Menthol migration to the filter and mainstream was measured under constant conditions for 80 days with intervals of 20 days. In the comparison between flue cured types, there were significant differences in the filter parts, as follows. KFUB1(34.4 %) KFUA4(37.4 %), KFLC1(43 %) and KFLCD4 were 55.7 %. In the comparision between other types of leaf tobaccos, KFUB1 was 34.4 % and KET was 52.6 % at filter parts. In the methol transfer to mainstream smoke was $16.5{\sim}24.2\;%$. The menthol migration to filters was measured based on the moisture content of $11{\sim}15\;%$ for the KFUB1 after storing it for 80 days. The menthol migrations were $36{\sim}40\;%$ at the moisture content of $11{\sim}15\;%$, respectively. The transfers to mainstream smoke were $12.8{\sim}15.8\;%$.

• Advances in materials Research
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• v.2 no.1
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• pp.51-64
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• 2013

Enantioselective macromer of trimesic acid was prepared using S(-) menthol with trimesoyl chloride on polyimide (PI) ultrafiltration membrane. The chemical composition of macromer as well as polyimide ultrafiltration membrane was determined by ATR-FTIR Spectroscopy. The optical resolution of chiral alcohols was performed in pressure driven process. The effect of monomer solutions concentration, effect of air-drying time of S(-) menthol solution, effect of reaction time, effect of operating pressure, effect of feed concentration of racemate on the performance of macromer was studied. The synthesised material exhibits separation of chiral alcohols (menthol ~23% and sobrelol ~21%).

• Natural Product Sciences
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• v.16 no.3
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• pp.180-184
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• 2010

The simple and accurate method was established for the simultaneous determination of (-)-menthone and (-)-menthol in Menthae herba obtained from Korea and China. A quantitative analysis was performed with a gas chromatography-flame ionization detector and reference compounds were separated on a capillary HP-Innowax column (30 m $\times$ 0.23 mm, 0.50 ${\mu}m$, Agilent, MA, USA). The correlation coefficients of the compounds showed good linearity ($r^2$ > 0.9997) over the linear range. The precision, repeatability and stability showed less than 1.7% of relative standard deviation (RSD) values for two compounds. Recovery rates were within the range of 95.72 - 103.76%. The method was applied successfully to analyze 15 samples of Menthae herba and achieved sufficient and specific separation of reference compounds. The principal component analysis (PCA) exhibited the classification of 15 samples according to their locations of origin.

• KSBB Journal
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• v.8 no.5
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• pp.495-503
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• 1993

To investigate the characteristics of growth and oil production of peppermint cells during a batch culture, cells derived from peppermint callus was cultivated in an air bubble reactor. During the batch culture, effects of inoculum size, abiotic stress, yeast elicitor, and two stage culture on the cell growth, the productivity of oleolesin, and the formation of flavor components were determined and also the sugar concentrations and kinetics of cell growth were analyzed. Among the various sizes of inoculum, the culture with 2.0% packed cell volume inoculum showed the optimum condition for cell growth in the proposed bioreactor, and the cell yield and essential oil production reached to 5.7g/1 and 0.109g/1, respectively. When the abiotic stress of daily 8hr dark and $10^{\circ}C$ cold treatments were given to the culture cell growth decreased but essential oil production increased to 0.546g/l. In a modified Lin-Staba medium in which 100mg/l yeast extract as an elicitor was added to the culture, the cell growth and oil production increased, and menthol content was 22.5% of oil. In the two stage culture, in which the basic culture conditions of 27$^{\circ}C$, light, and without elicitor were employed during the first six days followed by the second stage with daily 8hr treatment of cold and dark condition, and also with yeast extract as an elicitor, cell growth decreased after eight days, essential oil production was not increased, and menthol was not detected. Dry cell yield was 0.38g dry cell/g sugar and specific growth rate was 0.25 day-1. The major terpenoid in the oil was not the menthol but pulegone and piperitone, precursors of menthol were accumulated. However, when yeast elicitor was added, menthol was produced to the level of 22.5% which was the highest value in the peppermint cell culture reported so far.