• The Journal of Internal Korean Medicine
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• v.35 no.1
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• pp.37-49
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• 2014

Objectives & Methods : The objective of this study was to evaluate the single oral dose toxicity of Chongmyung-tang (CMT) in ICR mice. Korean traditional herbal prescription CMT has traditionally been used as a neuroprotective for treatment of learning disability and memory improvement. CMT, lyophilized aqueous extracts (yield=9.7%) were administered to female and male mice with oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) Guidelines. Animals were monitored for mortality, changes in body weight, clinical signs and gross observation during 14 days after administration upon necropsy; organ weight and histopathology of 14 principle organs were examined. Results : We could not find any CMT extracts treatment related mortalities, clinical signs, changes in body and organ weight, or gross and histopathological observations against 14 principle organs up to 2,000 mg/kg in both female and male mice, except for some accidental sporadic findings which did not show any obvious dose-relations and most of which also demonstrated in both the female and male vehicle control mice in this experiments. Conclusions : Based on the results of this experiment, the 50% lethal dose ($LD_{50}$) and approximate lethal dose (ALD) of CMT extracts after single oral treatment in female and male mice can be considered to be over 2,000 mg/kg, and is likely to be safe in humans.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.5
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• pp.738-742
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• 2005

This study was designed to investigate the anti-dementia effects of acorn (Quercus acutissima CARR.) in brain of the mouse. Dementia model was induced by administration of scopolamin (30 mg/kg BW) Male ICR mouse $(30{\pm}2g\;BW)$ were fed basal diet (control group), and experimental diets (AP-5 and AP-10 groups) added $5\%\;and\;10\%$ of dried acorn powder to basal diet for 8 months. Acetylcholine content significantly increase in AP-5 and AP-10 groups ($4.2\%\;and\;11.3\%$, respectively) compared with control group. Acetylcholinesterase activities were significantly inhibited ($13.5\%\;and\;17.6\%$, respectively) in brain of AP-5 and AP-10 groups. Monoamine oxidase-B (MAO-B) activities were significantly inhibited ($10.0\%\;and\;12.7\%$, respectively) in brain of AP-S and AP-10 groups. These results suggest that acorn (Q. acutissima CARR.) may play an effective role in an attenuating various age-related changes such as dementia including learning and memory impairments in brain.

• The Korean Journal of Physiology and Pharmacology
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• v.24 no.1
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• pp.11-18
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• 2020

The present study was aimed to explore the neuroprotective role of imatinib in global ischemia-reperfusion-induced cerebral injury along with possible mechanisms. Global ischemia was induced in mice by bilateral carotid artery occlusion for 20 min, which was followed by reperfusion for 24 h by restoring the blood flow to the brain. The extent of cerebral injury was assessed after 24 h of global ischemia by measuring the locomotor activity (actophotometer test), motor coordination (inclined beam walking test), neurological severity score, learning and memory (object recognition test) and cerebral infarction (triphenyl tetrazolium chloride stain). Ischemia-reperfusion injury produced significant cerebral infarction, impaired the behavioral parameters and decreased the expression of connexin 43 and phosphorylated signal transducer and activator of transcription 3 (p-STAT3) in the brain. A single dose administration of imatinib (20 and 40 mg/kg) attenuated ischemia-reperfusion-induced behavioral deficits and the extent of cerebral infarction along with the restoration of connexin 43 and p-STAT3 levels. However, administration of AG490, a selective Janus-activated kinase 2 (JAK2)/STAT3 inhibitor, abolished the neuroprotective actions of imatinib and decreased the expression of connexin 43 and p-STAT3. It is concluded that imatinib has the potential of attenuating global ischemia-reperfusion-induced cerebral injury, which may be possibly attributed to activation of JAK2/STAT3 signaling pathway along with the increase in the expression of connexin 43.

• The Korea Journal of Herbology
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• v.31 no.1
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• pp.7-15
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• 2016

Objectives : Polygalae Radix (POL) has an ameliorating effect on learning and memory impairment caused by cerebral hypoperfusion. In regard to POL's action mechanism, this study was carried out to investigate the effects of POL on oxidative damage and neuronal apoptosis induced by cerebral hypoperfusion in rats.Methods : The cerebral hypoperfusion was induced by permanent bilateral common carotid artery occlusion (pBCAO) in Sprague-Dawley rats. POL was administered orally once a day (130 mg/kg of water-extract) for 28 days starting at 4 weeks after the pBCAO. Superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels in the brain tissue were measured using ELISA method. Expressions of 4-hydroxynonenal (4HNE) and 8-hydroxy-2'- deoxyguanosine (8-OHdG) were observed using immunohistochemistry. In addition, neuronal apoptosis was evaluated with Cresyl violet staining, TUNEL labeling, and immunohistochemistry against Bax and caspase-3.Results : POL treatment significantly increased SOD activities and significantly reduced MDA levels in the cerebral cortex. The up-regulations of 4HNE and 8-OHdG expression caused by pBCAO in the CA1 of hippocampus were significantly attenuated by POL treatment. POL treatment also restored the reduction of CA1 thickness and CA1 neurons caused by pBCAO and significantly attenuated the apoptotic markers including TUNEL-positive cells, Bax, and caspase-3 expression in the CA1 of hippocampus.Conclusions : The results show that POL attenuated the oxidative damage in brain tissue and neuronal apoptosis in the hippocampus caused by the cerebral hypoperfusion. It suggests that POL can be a beneficial medicinal herb to treat the brain diseases related to cerebral hypoperfusion.

• Journal of Korean Society of Archives and Records Management
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• v.11 no.1
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• pp.139-159
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• 2011

This study investigated the perceived significance of laboratory(lab) notebooks and the creation and maintenance of them from the perspectives of graduate students and professors in science fields. Semi-structured interviews of three professors and four graduate students were conducted. The interviewees were from three university laboratories of physics, chemistry and biological science. While professors perceived administrative continuity to be a reason for creating lab notebooks, students valued lab notebooks as a memory, and emphasized learning professional knowledge and skills through the recordkeeping practice. Students understood the ownership of lab notebooks and professors wanted to keep lab notebooks until they retire. Interviewees focused more on the content of lab notebooks rather than the form and the structure. They were also not willing to adopt an electronic lab notebook system. Students learned how to create lab notebooks through a course, their current or former academic advisors, seniors in the lab or well-written sample lab notebooks. In particular, students have learned how to create lab notebooks within their laboratories, which are considered to be communities of practice. In this sense, professors responsible for managing laboratories play an important part in teaching students the recordkeeping practice. University archives should also be more concerned with managing and preserving lab notebooks created from their universities.

• Geophysics and Geophysical Exploration
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• v.26 no.3
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• pp.126-137
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• 2023

In this study, a recurrent neural network (RNN) was employed as a methodological approach to classify dolphin click signals derived from ocean monitoring data. To improve the accuracy of click signal classification, the single time series data were transformed into fractional domains using fractional Fourier transform to expand its features. Transformed data were used as input for three RNN models: long short-term memory (LSTM), gated recurrent unit (GRU), and bidirectional LSTM (BiLSTM), which were compared to determine the optimal network for the classification of signals. Because the fractional Fourier transform displayed different characteristics depending on the chosen angle parameter, the optimal angle range for each RNN was first determined. To evaluate network performance, metrics such as accuracy, precision, recall, and F1-score were employed. Numerical experiments demonstrated that all three networks performed well, however, the BiLSTM network outperformed LSTM and GRU in terms of learning results. Furthermore, the BiLSTM network provided lower misclassification than the other networks and was deemed the most practically appliable to field data.

• Korean Journal of Food Science and Technology
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• v.44 no.4
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• pp.428-433
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• 2012

Probiotics and their products, such as yogurt and cheese have been widely consumed in many countries with proven health benefits including anti-microbial activity and anti-diarrheal activity. LHFM (Lactobacillus helveticus - fermented milk) is a processed skim milk powder, fermented by a probiotics, L. helveticus IDCC3801. In the present study, we aimed to investigate the neuroprotective effects and the cognitive improvements of LHFM. LHFM itself did not show any cytotoxicity to the human neuroblastoma cell line, SH-SY5Y; however, it dose-dependently protected against glutamate-induced neuronal cell death. LHFM also attenuated scopolamine-induced memory deficit in Y-maze and Morris-water maze. In the analysis of hippocampus after a behavior test, LHFM significantly increased the acetylcholine level and also inhibited acetylcholine esterase activity. Therefore, the raised acetylcholine release partially contributes to the improvement of learning and memory by a treatment with LHFM. These results suggest that LHFM is an effective material for prevention or improvement of cognitive impairments caused by neuronal cell damage and central cholinergic dysfunction.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.20 no.1
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• pp.163-169
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• 2020

Recently, research on automation and unmanned operation of machines in the industrial field has been conducted with the advent of AI, Big data, and the IoT, which are the core technologies of the Fourth Industrial Revolution. The machines for these automation processes are controlled based on the data collected from the sensors attached to them, and further, the processes are managed. Conventionally, the abnormalities of sensors are periodically checked and managed. However, due to various environmental factors and situations in the industrial field, there are cases where the inspection due to the failure is not missed or failures are not detected to prevent damage due to sensor failure. In addition, even if a failure occurs, it is not immediately detected, which worsens the process loss. Therefore, in order to prevent damage caused by such a sudden sensor failure, it is necessary to identify the failure of the sensor in an embedded system in real-time and to diagnose the failure and determine the type for a quick response. In this paper, a deep neural network-based fault diagnosis system is designed and implemented using Raspberry Pi to classify typical sensor fault types such as erratic fault, hard-over fault, spike fault, and stuck fault. In order to diagnose sensor failure, the network is constructed using Google's proposed Inverted residual block structure of MobilieNetV2. The proposed scheme reduces memory usage and improves the performance of the conventional CNN technique to classify sensor faults.

• The Journal of the Korea Contents Association
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• v.15 no.4
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• pp.29-44
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• 2015

Digital Signage, a way of advertising or delivering information to viewers through digital displays, has expanded from being just an advertising channel in public places. Recently, it has become widely prevalent in restaurants and retail stores. Despite its wide expansion, digital signage is limited to specific usages and services and the devices it uses are also quite expensive. This study introduces a stick-type digital signage product that operates on Android OS, which addresses all the weaknesses of digital signage with much more reasonable pricing and stable operation. For stability, performance tests were executed on the hardware and applications. The results for hardware performance were extremely promising, as each scenario's maximum performance results, measured by Load Runner programs, reached target indexes. Also, as a result of the usability test, all participants, including non-digital signage system users (novices), were able to easily learn all the tasks. As a result of user satisfaction survey, positive responses were exhibited for ease of learning and usability (LEU), helpfulness and problem solving capabilities (HPSC), affective aspect and multimedia properties (AAMP), commands and minimal memory load (CMML), and control and efficiency (CE).

• Herbal Formula Science
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• v.25 no.4
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• pp.483-497
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• 2017

Background : Behavioral stress has been suggested as one of the significant factors that is able to disrupt physiological systems and cause depression as well as changes in various body systems. The stressful events can alter cognition, learning, memory and emotional responses, resulting in mental disorders such as depression and anxiety. Results : We used a restraint stress model to evaluate the alteration of behavior and stress-related blood parameter. The animals were randomly divided into two groups of five animals each group. Furthermore, we assessed the change of body weight to evaluate the locomotor activity as well as status of emotional and anxiety in mice. After 7 days of restraint stress, the body weight had significantly decreased in the restraint stress group compared with the control group. We also observed stress-associated behavioral alterations, as there was a significant decrease in open field and forced swim test, whereas the immobilization time was significantly increased in the stress group compared to the control group. We observed the morphological changes of neuronal death and microglia by immunohistochemistry and western blot. In our study restraint stress did not cause change in neuronal cell density in the frontal cortex and CA1 hippocampus region, but there was a trend for an increased COX-2 and iNOS protein expression and microglia (CD11b) in brain, which is restraint stress. Conclusion : Our study, there were significant alterations observed in the behavioral studies. We found that mice undergoing restraint stress changed behavior, confirming the increased expression of inflammatory factors in the brain.