• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2003년도 봄 학술발표회 논문집
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• pp.912-917
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• 2003

In this paper a new truss modeling technique for describing the beam shear resistance mechanism is proposed based on the reinterpretation of the well-known relationship between shear and the rate of change of bending moment in a reinforced concrete beam subjected to combined shear and moment loads. The core of the model is that a new perspective on the shear resistance can be gained by viewing the internal stress filed in terms of the superposition of two base components of shear resistance; arch action and beam action. The arch action can be described as a simple tied-arch which is consisted of a curved compression chord and a tension tie of the longitudinal steel, while the beam action between the two chords can be modeled as a membrane shearing element with forming a smeared truss action. The compatibility of deformation associated to the two action is taken into account by employing an experimental factor or internal state force factor a. Then the base equation of V=dM/dx is numerically duplicated. The new model was examined by the 362 experimental results. The shear strength predicted by the internal force state factor a show better correlation with the tested values than the present shear design.

• 한국수소및신에너지학회논문집
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• 제23권2호
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• pp.150-155
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• 2012

Polymer Electrolyte Membrane Fuel Cell (PEMFC) stack power output is needed to be approximately 100 kW to meet the requirements of automotive applications. In order to secure the electric safety for drivers, passengers and mechanics, it is very important to understand phenomena of an electric insulation in a fuel cell vehicle. In this study, we studied the electric insulation properties and the insulation resistance of stack, system and vehicle in the field of fuel cell was estimated at the applied voltage of 500 V, respectively. Also we discussed the insulation factors such as the conductivity of coolant, the element of vehicle design and the intrinsic resistance of the vehicle components.

• BMB Reports
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• 제41권10호
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• pp.728-732
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• 2008

FoxO3a is a member of the forkhead box class O (FoxO) transcription factor family and an important regulator of apoptosis. This work aimed to elucidate the involvement of FoxO3a in transforming growth factor-${\beta}1$(TGF-${\beta}1$)-induced apoptosis in FaO rat hepatoma cells. TGF-${\beta}1$ caused a time-dependent activation of FoxO3a and a subsequent increase in FoxO response-element-containing luciferase reporter activity, which was Akt-sensitive. The FaO cells stably transfected with a wild type FoxO3a were more susceptible to the formation of apoptotic bodies, populations of sub-G1 apoptotic cells, and collapse of the mitochondrial-membrane potential triggered by TGF-${\beta}1$. In contrast, transfection with small-interfering RNA (siRNA) oligonucleotide specific for FoxO3a significantly inhibited caspase activation in FaO cells treated with TGF-${\beta}1$. It thus appears that FoxO3a plays a crucial mediatory role in the TGF-${\beta}1$ signaling pathway leading to apoptosis.

• 한국공간구조학회논문집
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• 제8권2호
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• pp.105-113
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• 2008

쉘형 구조물은 외력에 대해 효과적으로 저항할 수 있어, 두께를 얇게하면서 대공간 구조물을 만들 수 있는 장점이 있다. 이러한 장점은 구조형태에 크게 의존한다. 그러므로 많은 설계자들은 최적 형태를 설계에 반영하고자 하지만, 이는 간단치 않다. 지금까지 보다 최적의 형태를 얻기 위한 많은 기법들이 발표되어 왔고, 이들은 각각 장단점을 나타낸다. 본 논문에서는 최적의 곡면을 얻기 위해 기하학적 비선형을 고려한 유한요소법을 이용한 비교적 간단한 방법을 제안한다. 이러한 방법을 이용하여 다양한 쉘형 곡면에 적용하여 최적곡면을 얻고, 이를 비교한다.

• 한국소음진동공학회논문집
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• 제15권5호
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• pp.595-603
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• 2005

In this work, the multiple dynamic absorber( MDA ) is introduced to reduce several vibration modes of shadow mask simultaneously and its design method is developed from the theory of the simple dynamic absorber. When designing the dynamic absorber, there are three significant design parameters such as mass, damping ratio and tuning frequency. Therefore the sensitivity analysis for those parameters has been executed in order to find out the design criteria of multiple dynamic absorber using the finite element model of shadow mask. The multiple dynamic absorber(MDA) designed by the proposed method is tested theoretically and experimentally to estimate the efficiency of vibration reduction. From the results, it is verified that the method is feasible to apply the system having the multiple nitration modes and more efficient than the thin wire-type damper used commercially to reduce the vibration of shadow mask.

• 대한의용생체공학회:의공학회지
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• 제24권5호
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• pp.421-425
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• 2003

한 개 척추 운동 분절에 대한 유한요소 모델을 이용하여 수직 충격력이 가해질 경우 발생되는 척추 경막의 교합을 해석 하였다. 교합은 수직 충격력의 지속 시간 변화에 따른 골편의 이동으로 인한 척추 경막의 단면적 변화를 계산하여 계량화 하였다. 계산 결과 8∼12ms 의 충격 지속 시간에서 골편의 이동으로 인한 가장 큰 척추 경막의 단면적 변화가 나타났으며 $\Delta$t = 400 ms의 경우에서는 4%만의 척추 경막의 단면적 변화가 나타났다, 이와 같은 해석 결과는 6kN의 수직 정 하중이 가해질 경우에 발생되는 량이 유사한 값으로 이 정도의 충격 지속 시간은 준 정 하중 상태와 유사함을 보이고 있었다.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1998년도 봄 학술발표회 논문집
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• pp.93-100
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• 1998

The basic systems of spatial structures such as shells, membrane, cable-nets and tensegrity structure have been developed to create the large spaces without column. These structures may have large freedom in scale and form, and especially tensegrity structures are received much attention from the view points of their light weight and aesthetics. But There re some difficulties concerning structural stability, surface formation and construction method. One of the way to solve these problems reasonably is a combination of tensile members and rigid members. A structural system based on this concept is referred to as the "HTS ( Hybrid Tension Structure )". This is a type of flexible structural system which is unstable initially, because the cable material has little initial rigidity. As cable - dome hybrid structures is a type of HTS, the initial stress for the self- equilibrated system having stable state have to be introduced. To determine initial stress having stable state, the shape finding analysis is required before the stress - deformation analysis. In this paper, the primary objective is to derive the nonlinear finite element formula of cable and truss members considering geometric nonlinearity for shape finding of cable-dome, and to propose the method to decide the initial stress by the shape analysis of cable-dome hybrid structure with the self-equilibrated state.

• Molecules and Cells
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• 제20권2호
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• pp.196-200
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• 2005

The neurofibromatosis type2 (NF2) tumor suppressor gene product, merlin, is structurally related to the ezrin-radixin-moesin (ERM) family of proteins that anchor the actin cytoskeleton to specific membrane proteins and participate in cell signaling. However, the basis of the tumor suppressing activity of merlin is not well understood. Previously, we identified a role of merlin as an inhibitor of the Ras-ERK signaling pathway. Recent studies have suggested that phosphorylation of merlin, as of other ERM proteins, may regulate its function. To determine whether phosphorylation of merlin affects its suppression of Ras-ERK signaling, we generated plasmids expressing full-length merlin with substitutions of serine 518, a potential phosphorylation site. A substitution that mimics constitutive phosphorylation (S518D) abrogated the ability of merlin to suppress effects of the Ras-ERK signaling pathway such as Ras-induced SRE transactivation, Elk-mediated SRE transactivation, Ras-induced ERK phosphorylation and Ras-induced focus formation. On the other hand, an S518A mutant, which mimics nonphosphorylated merlin, acted like wild type merlin. These observations show that mimicking merlin phosphorylation impairs not only growth suppression by merlin but also its inhibitory action on the Ras-ERK signaling pathway.

• 미생물학회지
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• 제30권5호
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• pp.333-338
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• 1992

Saccharomyces cerevisiae 의 mother cell과 daughter cell 의 연결 부위의 원형질막 바로 안쪽에 존재하는 10-nm filament ring 은 세포형태 형성과정에 중요한 역할을 하리라 간주되나 그 명확한 생성기작과 기능은 밝혀지지 않았다. 본연구에서는 CDC12 유전자로부터 gene fusion technique 을 이용하여 CDC12 단백질을 만들고 이로부터 항체를 형성하였다. 이항체를 이용하여 10-nm filament ring 의 생성기작과 기능에 대하여 연구하였다. 그 결과 CDC12 단백질은 cell cycle 전주기동안 항상 정이되나 bud 가 나오기 바고 직전에 bud 가 나올 부위에 polymerization 되었다가 세포질분열 바로 직후에 unpolymerization 되며 cytoskeletal element 의 일종인 actin 과는 무관하게 행동하는 건이 밝혀졌다. 이러한 10-nm filament 는 bud 가 나올 부위의 올바른 선정과 세포질 분열에 중요한 역할을 하리라 간주된다.

• Bulletin of the Korean Chemical Society
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• 제32권3호
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• pp.768-778
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• 2011

Calixpyrroles and related macrocycles are non-planer synthetic anion receptors that have attracted considerable attentions in recent years. Although the synthesis of calix[4]pyrrole (known as meso-octamethylporphyrinogen) was reported more than 100 years ago, the anion binding properties were first discovered in 1996. The simple calix[4]pyrroles can be synthesized in single step in high yield by condensation of pyrrole with acetone. The compounds showed preferential binding for halide anions including fluoride, phosphate, carboxylate, and chloride in organic media. Efforts to improve the anion affinity of calix[4]pyrrole and to enhance its selectivity have led to the synthesis of a variety of new calixpyrrole derivatives. Among the various modifications, introduction of straps on one side of the calix[4]pyrroles are the most effective. Incorporation of aromatic rings other than pyrroles also exhibited interesting binding behaviour. Introduction of signalling units as part of the strapping element enable to detect the anions on chromogenic or fluorogenic fashion. Finding of the anion transport properties across the membrane and cytotoxic effects of the calix[4]pyrroles open new window for calixpyrrole-related research. The polymer-incorporated systems have also been employed as anion complexants in solvent-solvent extraction. These old, yet easy-to-make macrocycles have well advanced more recently with the discovery of the ion-pair complexation properties. In this review, the synthetic developments and anion binding properties of calixpyrroles for the last decades will be discussed and will cover the advances in calixpyrrole chemistry.