• KSBB Journal
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• 제13권6호
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• pp.718-723
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• 1998

The effect of glycyrrhizin on melanoma cells was investigated. DNA fragmentation in cultured melanoma cells was promoted by the addition of glycyrrhizin in a dose dependent manner. Administration(i.m.) of glycyrrhizin to Mel/ret transgenic mice resulted in apoptosis induction, reduction of mitochondrial transmembrane potential in melanoma cells. Decreased B220+ B cells were recovered by the treatment of glycyrrhizin in splenocytes and mesenteric lymph node cells, while Thy-1+ T cells were not influenced. Results suggested that glycyrrhizin acted as an inducer of apoptosis of melanoma cells and an immuno-potentiator via recovered B lymphocyte population in Mel/ret transgenic mice.

• Journal of Acupuncture Research
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• 제18권4호
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• pp.101-115
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• 2001

Objective : To characterize the antitumorigenic potential of Apamin, one of the major components of bee venom, its effects on cell proliferation and the mitogen-activated protein kinase (MAPK) signal transduction pathway were characterized using the human melanoma cell line SK-MEL-2. Methods & Results : Cell counting analysis for cell death demonstrated that consistent with a previous results, SK-MEL-2 cells treated with $0.5-2.0{\mu}g/ml$ of Apamin showed no recognizable cytotoxic effect whereas detectable induction of cell death was identified at concentrations over $5.0{\mu}g/ml$. [3H]thymidine incorporation assay for cell proliferation demonstrated that DNA replication of SK-MEL-2 cells is inhibited by Apamin in a dose- and time-dependent manner. To explore whether Apamin-induced growth suppression is associated with the MAPK signaling pathway, phosphorylation of Erk, a function mediator of MAPK growth-stimulating signal, was examined Western blot assay using a phospho-specific Erkl/2 antibody. A significant increase of Erkl/2 phosphorylation level was observed in Apamin-treated cells compared with untreated control cells. Qantitative RT-PCR analysis revealed that Apamin inhibit expression of MAPK downstream genes such as c-Jun, c-Fos, and cyclin D1 but not expression of MAPK pathway component genes including Ha-Ras, c-Raf-1, MEK1, and Erk. Conclusion : It is strongly suggested that the antitumorigenic activity of Apamin might result in part from its inhibitory effect on the MAPK signaling pathway in human melanoma cells SK-MEL-2.

• Archives of Pharmacal Research
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• 제29권3호
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• pp.224-234
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• 2006

We employed human SK-MEL-28 cells as a model system to identify cellular proteins that accompany N-(4-methyl)phenyl-O-(4-methoxy)phenyl-thionocarbamate (MMTC)-induced apoptosis based on a proteomic approach. Cell viability tests revealed that SK-MEL-28 skin cancer cells underwent more cell death than normal HaCaT cells in a dose-dependent manner after treatment with MMTC. Two-dimensional electrophoresis in conjunction with matrixassisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis or computer matching with a protein database further revealed that the MMTC-induced apoptosis is accompanied by increased levels of caspase-1, checkpoint suppressor-1, caspase-4, NF-kB inhibitor, AP-2, c-Jun-N-terminal kinase, melanoma inhibitor, granzyme K, G1/S specific cyclin D3, cystein rich protein, Ras-related protein Rab-37 or Ras-related protein Rab-13, and reduced levels of EMS (oncogene), ATP synthase, tyrosine-phosphatase, Cdc25c, 14-3-3 protein or specific structure of nuclear receptor. The migration suppressing effect of MMTC on SK-MEL-28 cell was tested. MMTC suppressed the metastasis of SK-MEL-8 cells. It was also identified that MMTC had little angiogenic effect because it did not suppress the proliferation of HUVEC cell line. These results suggest that MMTC is a novel chemotherapeutic and metastatic agents against the SK-MEL-28 human melanoma cell line.

• 한국약용작물학회지
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• 제12권3호
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• pp.255-261
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• 2004

21 종의 생약 추출물을 선정하여 $5\;{\mu}g/ml$의 농도에서 SK-MEL-28 cell 에 대한 세포독성을 조사한 결과 목단피의 메탄올 추출물이 74.3%의 성장율을 보였다. 활성 물질을 찾기 위하여 목단피로부터 silica gel column chromatography를 실시하여 hexane 분획과 EtOAc 분획에서 충 5개의 화합물을 분리하였고 $mp,\;UV,\;IR,\;^1H-NMR,\;^{13}C-NMR$ 등 각종 물리, 화학적 data로부터 그 구조를 paeonol (1), benzoylpaeoniflorin (2), benzoic acid (3), 2,5-dihydroxy-4-methoxy-acetophenone (4), paeoniflorin (5)으로 동정하였다. 분리한 물질을 human 피부 암세포인 SK-MEL-28 세포주에 대하여 $10\;{\mu}g/ml$의 농도에서 SRB방법으로 세포독성을 측정한 결과 compound 4가 $ED_50$ 값이 $5.92\;{\mu}g/ml$로 가장 좋은 세포독성을 나타내었다. 이결과는 compound 4가 SK-MEL-28 melanoma 세포주에 대한 새로운 항암 후보 물질임을 제시한다.

• 한방안이비인후피부과학회지
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• 제37권1호
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• pp.1-16
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• 2024

Objectives : We aimed to study the effect of Smilax China L.(SCL), which has anti-inflammatory, antioxidant, and anticancer effects, on the growth of skin cancer cells. Methods : HaCaT cells, a normal human cell line, and skin cancer cells including A431, SK-MEL-5 and SK-MEL-28 cells were treated with Smilax China L. ethanol extract(SCL-EtOH) at concentrations of 5, 10, 20 and 40㎍/㎖. Meanwhile, JB6 Cl41, a normal mouse epithelial cell line, was treated with epidermal growth factor(EGF) and phorbol 12-myristate 13-acetate(TPA), an inflammatory factor, to induce cell transformation and treated with SCL-EtOH. In addition, we treated SK-MEL-5 and SK-MEL-28 cells with SCL-EtOH at various concentrations and checked the effect on the cell cycle. Results : As a result, it showed no toxicity to HaCaT cells up to the highest concentration of 40㎍/㎖, and significant cell growth inhibition to A431, SK-MEL-5 and SK-MEL-28 cells in a time- and concentration-dependent manner. In addition, as a result of checking the shape of skin cancer cells according to SCL-EtOH treatment, it was observed that as the concentration increased, the number of normally attached and growing cells decreased and the shape of the cells changed. Colony formation was significantly reduced in a concentration-dependent manner in JB6 Cl41 cells treated with EGF or TPA. Flow cytometry analysis with propidium iodide(PI) staining showed that SCL-EtOH induced the G2/M phase arrest. We further confirmed the decrease in Cyclin B1 expression and increase in p27 expression associated with the G2/M phase of the cell cycle through western blot analysis. Flow cytometry analysis confirmed that SCL-EtOH induced cell apoptosis. Furthermore, through Western blot analysis, it was observed that the expression of cleaved-caspase-7, which is related to apoptosis, increased. Finally, it was confirmed that the expression of COX-2, an inflammatory marker protein, decreased in a concentration-dependent manner with SCL-EtOH. Conclusions : Through the above results, we have established a basis for applying SCL to the treatment of skin cancer.

• 인터넷정보학회논문지
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• 제1권2호
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• pp.49-59
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• 2000

컴퓨터의 발달과 인터넷 사용자의 증대로 자연어 처리의 연구에 관한 관심이 증대되고 있다. 그러나 대부분의 연구가 자연어 분석 및 이해에 집중되고 있어 자연어 생성에 관한 연구는 주목을 받지 못해 왔으며 자연어 생성을 자연어 분석의 역 과정으로 간단하게 생각하는 경향마저도 있다. 하지만 Web상에서의 다국어간 번역 자연어 인터페이스 자연어 검색 시스템 등 자연어처리에 관한 필요성이 증가함에 따라 자연어 생성의 필요성도 자연히 증가하고 있는 실정이며 좀 더 체계적인 자연어 생성 시스템 개발을 위해서는 자연어 생성에 관한 보다 구체적인 알고리즘에 관한 연구가 필요하다. 본 논문에서는 영문 생성에 있어서 보다 자연스러운 문장을 생성하기 위한 알고리즘을 제안하며 특히 Igor Mel'uk (Mel'uk ＆ Zholkovsky, 1988)의 어휘 함수(LFs)를 이용한 어휘 결합을 통하여 절 길이의 설명문을 생성하는 영문 생성기의 구현에 대하여 논한다.

• Journal of information and communication convergence engineering
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• 제1권4호
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• pp.209-212
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• 2003

In this paper, we proposed new speech feature parameter through parts-based feature extraction of speech spectrum using Non-Negative Matrix Factorization (NMF). NMF can effectively reduce dimension for multi-dimensional data through matrix factorization under the non-negativity constraints, and dimensionally reduced data should be presented parts-based features of input data. For speech feature extraction, we applied Mel-scaled filter bank outputs to inputs of NMF, than used outputs of NMF for inputs of speech recognizer. From recognition experiment result, we could confirm that proposed feature parameter is superior in recognition performance than mel frequency cepstral coefficient (MFCC) that is used generally.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.273.1-273.1
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• 2002

Temperature change is one of the major environmental factors to influence human skin. However. the relationship between temperature and melanogenesis has received little attention. In the present study. we investigated the effects of temperature change including heat shock on melanogenesis using a mouse melanocyte cell line, Mel-Ab. Our results demonstrated that cells maintained at 37$^{\circ}C$ showed maximal melanin synthesis. (omitted)

• Journal of Acupuncture Research
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• 제18권1호
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• pp.129-145
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• 2001

Objectives : To characterize the antitumorigenic potential of three representative bee venom components, Melittin, Apamin, and Phospholipase A2, their effects on cell proliferation and apotosis of the human melanoma cell line SK-MEL-2 were analyzed using molecular biological approaches. Methodes & Results : To determine the doses of the drugs that do not induce cytotoxic damage to this cell line, cell viability was examined by MTT assay. While SK-MEL-2 cells treated with 0.5 - 2.0㎍/㎖ of each drug showed no recognizable cytotoxic effect, marked reductions of cell viability were detected at concentrations over 5.0㎍/㎖. [3H]thymidine incorporation assay for cell proliferation demonstrated that DNA replication of SK-MEL-2 cells is inhibited by Apamin and Phospholipase A2 in a dose-dependent manner. Consistent with this result, the cells were accumulated at the G1 phase of the cell cycle after treatment with Apamin and Phospholipase A2, whereas no detectable change in cell proliferation was identified by Melittin treatment. In addition, tryphan blue exclusion and flow cytometric analyses showed that all of these drugs can trigger apoptotic cell death of SK-MEL-2, suggesting that Melittin, Apamin, and Phospholipase A2 have antitumorigenic potential through the suppression of cell growth and/or induction of apoptosis. Qantitative RT-PCR analysis revealed that Apamin and Phospholipase A2 inhibit expression of growth-promoting genes such as c-Jun, c-Fos, and Cyciin D1. Furthermore, Phospholipase A2 induced tumor suppressors p53 and p21/Wafl. In addition, all three drugs were found to activate expression of a representative apoptosis-inducing gene Bax while expression of apoptosis-suppressing Bcl-2 and Bcl-XL genes was not changed. Taken together, this study strongly suggests that Metittin, Apamin, and Phosphalipase A2 may have antitumorigenic activities, which are associated with its growth-inhibiting and/or apoptosis-inducing potentials.

• 한국음향학회지
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• 제25권1호
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• pp.36-42
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• 2006

본 논문에서는 화자검증 시스템의 성능향상을 위해서 주성분 분석 (PCA) 기반 Mel-Frequency Discrete Wavelet Coefficients (MFDWC) 추출방법을 제안한다. 제안된 방법에서는 멜척도 (Mel-scale)를 근사화한 각 레벨 (level)의 각 노드 (node) 에너지를 계산하기 위해 기존의 평균치 대신 주성분 분석을 이용한 첫 번째 eigenvector를 이용한다. 이 eigenvecto.의 제곱의 합은 1로서 일반적인 가중 함수 (weighting function)의 조건을 만족하고, 또한 각 화자마다 서로 다른 값을 갖게 되므로, 화자의 특징을 더 잘 나타내는 MFDWC를 추출할 수 있다. 화자검증은 Gaussian Mixture Model (GMM) 기반의 백그라운드 모델과 화자 모델과의 점수를 비교하는 이진 결정 (binary decision) 방법을 이용하여 Universal 백그라운드 모델 (UBM)과 각 화자 모델의 값을 프레임단위로 비교하여 대상 화자의 수락/거부 여부를 결정하는 방법을 채택하였다. 특징 파라미터에 따른 화자 검증 성능변화를 확인하기 위하여 제안된 화자종속 가중함수를 이용한 MFDWC를 특징 파라미터로 이용한 경우와 Mel-Frequency Cepstral Coefficients (MFCC), Linear Predictive Cepstral Coefficients (LPCC), 기존의 MFDWC를 특징 파라미터로 이용한 경우에 대하여 성능비교실험을 수행한 결과 각각 $0.80\%,\;5.14\%,\; 6.69\%$의 향상된 성능을 나타내어 제안한 방법의 유효성을 확인할 수 있었다.