• Journal of Electrical Engineering and Technology
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• v.3 no.3
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• pp.422-429
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• 2008

This paper presents compact low frequency ultra-wide band (UWB) sensor design and study of the partial discharge diagnosis by sensing electromagnetic pulse emitted from the partial discharge source with the newly designed UWB sensor. In this study, we designed a new type of compact low frequency UWB sensor based on microstrip antenna technology to detect both the low frequency and high frequency band of the partial discharge signal. Experiments of offline PD testing on medium voltage (22.9kV) underground cable mention the comparative results with the traditional HFCT as a reference sensor in the laboratory. In the series of comparative tests, the calibration signal injection test provided with the conventional IEC 60270 method and high voltage injection testing are included.

• Korean Journal of Microbiology
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• v.21 no.3
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• pp.156-162
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• 1983

Conditions for effcient formation and regeneration of protoplasts of Micromonospora rosaria and Micromonospora purpurea were investigated. The state of inoculm, culture stage and growth in a medium containing partially growth-inhibiting concentration of glycing have significant effects on portoplasting. A high frequency of regeneration (up to 30%) was accomplished with a hypertonic regeneration agar medium defined by Okanishi for Strptomyces. Using the optimal conditions for protroplasting and regeneration, protoplast fusion of auxotrophic M.rosaria was carried out. Polyethylene glycol 1,000 was chosen for fusogenic agent. When signgle auxotrophs were used, the recombinant frequency of auxortrophic markers varied from 1.3 to 3.2%. Using two double auxotrophs, the recombinant frequencies of 0.7-4.3% were obtained. Much lower frequencies(three or more orders of magnitude) were observed by the conventional matings.

• Journal of Plant Biotechnology
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• v.30 no.3
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• pp.233-239
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• 2003

We have developed a reliable and high-frequency genetic transformation and regeneration system via somatic embryogensis of Eleutherococcus sessiliflorus. Embryogenic callus obtained from seed were co- cultivated with Agrobacterium tumefaciens strain EHA101/pIG121Hm harboring genes for intron-$\beta$-glucoronidase(GUS), kanamycin and hygromycin resistance. Following co-cultivation, two types of samples(fine embrogenic calli and early globular embryo clusters) were cultivated on Murashige and Skoog(MS) medium containing 1 mg/L2.4-D for 3day in dark. Transient expression of GUS gene was found to be higher in the early globular embryo clusters than in the embryogenic calli. Also, co-cultivated period affected expression of GUS gene; the best result was obtained when globular embryo clusters were co-cultivated with Agrobacterium for 3 days. Subsequently, this callus transferred to selective MS medium containing 1mg/L2.4-D, 50mg/L kanamycin or/and 30mg/L hygromycin and 300mg/L cefortaxime. These embryogenic calls were subcultured to the same selection medium at every 2 weeks intervals. Approximately 24.5% of the early globular embryos co-cultivated with Agrobacterium for 3days produced kanamycin or/and hygromycin-resistant calli. Transgenic somatic embryos were converted into plantlets in half strength MS medium supplemented with 3mg/L GA$_3$ kanamycin and were confirmed by GUS histochemical assay and polymerase chain reaction analysis. Genomic Southem blot hybridization confirmed the incorporation of NPT II gene into the host genome.

• Plant Biotechnology Reports
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• v.2 no.1
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• pp.7-11
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• 2008

A simple, high-frequency and reproducible protocol for induction of adventitious shoot buds and plant regeneration from leaf-disc cultures of Jatropha curcas L. has been developed. Adventitious shoot buds were induced from very young leaf explants of in vitro germinated seedlings as well as mature field-grown plants cultured on Murashige and Skoog's (MS) medium supplemented with thidiazuron (TDZ) ($2.27{\mu}M$), 6-benzylaminopurine (BA) ($2.22{\mu}M$) and indole-3-butyric acid (IBA) ($0.49{\mu}M$). The presence of TDZ in the induction medium has greater influence on the induction of adventitious shoot buds, whereas BA in the absence of TDZ promoted callus induction rather than shoot buds. Induced shoot buds were multiplied and elongated into shoots following transfer to the MS medium supplemented with BA ($4.44{\mu}M$), kinetin (Kn) ($2.33{\mu}M$), indole-3-acetic acid (IAA) ($1.43{\mu}M$), and gibberellic acid ($GA_3$) ($0.72{\mu}M$). Well-developed shoots were rooted on MS medium supplemented with IBA ($0.5{\mu}M$) after 30 days. Regenerated plants after 2 months of acclimatization were successfully transferred to the field without visible morphological variation. This protocol might find use in mass production of true-to-type plants and in production of transgenic plants through Agrobacterium/biolistic-mediated transformation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.615-625
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• 1997

An efficient protocol for plant regeneration from protoplasts of the indica rice variety IR43 has been developed. The procedure involved plating of embryogenic suspension-derived protoplasts on the surface of a filter membrane overlaying agarose-embedded feeder cells. Lolium multiflorum cell suspensions were preferable to these of Oryza ridleyi as feeder cells and Lolium suspensions supported colony formation from up to 0.68％ of the protoplasts, depending on the age of cell suspensions. Plant regeneration frequency was significantly improved by using maltose alone or in a 1:1(w/w) combination with sucrose as carbohydrate source and a simple dehydration treatment using a high concentration of agarose in the regeneration medium. Medium containing maltose or maltose mixed with sucrose increased the plant regeneration frequency compared with medium containing sucrose alone. The plant regeneration frequency was increased to 30.7 to 70.7％ following dehydration treatment, while the non-treated controls showed a regeneration frequency of 3.1 to 30.6％. Protoplast-derived plants were transferred to the glasshouse, flowered with morphologically normal.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2003.11a
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• pp.824-830
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• 2003

The Power Flow Finite Element Method(PFFEM) offers very promising results in predicting the vibration responses of system structures, and the first PFFEM software, PFADS has been developed in Seoul National University for the vibration predictions and analysis of coupled system structures in medium-to-high frequency ranges. PFFEM is numerical method which solves energy governing equation using finite element technique for complicated structures where the exact solutions are not available. Through the upgrades, the current version PFADS R3 could cover the general beam and plate structures including various kinds of beam-plate rigid joints, spring-damper connection and rigid body connection within beam and plate in addition. This software is composed of three parts; translator, model converter and solver. The translator makes its own FE-model from bulk data of commercial FE software, and the model converter is used to convert FE-model to PFFE-model automatically. The solver calculates vibrational energy density and intensity for PFFE-model by solving global matrix equations of PFFEM. For the applications of PFADS R3, two vehicle models and a container model are examined with respect to major parameters, and reliable results are obtained.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.22 no.11
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• pp.1033-1041
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• 2012

Power flow analysis(PFA) is introduced for solving the noise and vibration analysis of system structures in medium-to-high frequency ranges. The vibration analysis software, $PFADS_{C++}$ R4 based on power flow finite element method(PFFEM) and the noise prediction software, $NASPFA_{C++}$ R1 based on power flow boundary element method(PFBEM) are developed. In this paper, the coupled PFFE/PFBE method is used to investigate the vibration and radiated noise of the rotary compressor. PFFEM is employed to analyze the vibrational responses of the rotary compressor, and PFBEM is applied to analyze the radiation noise around rotary compressor. The vibrational energy of the structure is used as an acoustic intensity boundary condition of PFBEM. Numerical simulations are presented for the rotary compressor, and reliable results have been obtained.

• Journal of Ship and Ocean Technology
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• v.8 no.4
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• pp.26-33
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• 2004

PFFEM software, PFADS has been developed for the vibration predictions and analysis of coupled system structures in medium-to-high frequency ranges. PFFEM is numerical method which solves energy governing equation using finite element technique for complicated structures where the exact solutions are not available. Through the upgrades, present PFADS R3 could cover the general beam and plate structures including various kinds of beam-plate rigid joints and other joint systems such as spring-damper junction and rigid bar connection. This software is composed of 3 parts; translator, model converter and solver. The translator makes its own FE-model from bulk data of commercial FE software, and the model converter is used to convert FE-model to PFFE-model automatically. The solver calculates vibrational energy density and intensity for PFFE-model by solving global matrix equations of PFFEM. For the applications of real transportation systems, a container ship model has been examined with respect to major parameters, and reliable results have been obtained.

• Journal of Sensor Science and Technology
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• v.29 no.1
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• pp.33-39
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• 2020

Typically, photoacoustic images are obtained in water or gelatin because the impedance of these mediums is similar to that of the human body. However, these acoustic mediums can have an additional mass effect that changes the resonance frequency of the transducer. The acoustic radiation impedance in air is negligible because it is very small compared to that of the transducer. However, the high acoustic impedance of mediums such as the human body and water is quite large compared to that of air, making it difficult to ignore. Specifically, in a case where the equivalent mass is very small, such as with a micro-machined ultrasound transducer, the additional mass effects of the acoustic medium should be considered for an accurate resonance frequency design. In this study, a piezoelectric micro-machined ultrasonic transducer (pMUT) was designed to have a resonance frequency of 10 MHz in the acoustic medium of water, which has similar impedance as the human body. At that time, the resonance frequency of the pMUT in air was calculated at 15.2 MHz. When measuring the center displacement of the manufactured pMUT using a laser vibrometer, the resonance frequencies were measured as 14.3-15.1 MHz, which is consistent with the finite element method (FEM) simulation results. Finally, photoacoustic images of human hair samples were successfully obtained using the fabricated pMUT.

• Horticultural Science & Technology
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• v.18 no.6
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• pp.811-814
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• 2000

Effects of kanamycin concentration on regeneration and rooting of transgenic 'McIntosh Wijcik' were investigated to establish the efficient Agrobacterium-mediated transformation system. Relatively high regeneration frequency of explants appeared even at the high concentration of $150mg{\cdot}L^{-1}$ kanamycin, but the regeneration frequency and the number of normal shoots decreased significantly at a concentration of higher than $100mg{\cdot}L^{-1}$ kanamycin in the gelrite-gellifying medium. Rooting response varied with the transgenic lines in the agar-solidifying medium supplemented with the different concentrations of kanamycin and they were grouped with the inhibition level at $30mg{\cdot}L^{-1}$ concentration. No correlation between copy number and root response was observed. The optimum concentrations of kanamycin for the regeneration of 'McIntosh Wijcik' apple in the medium gellified with gelrite and for indirect-selection of putative transformants in the rooting medium solidified with agar were found to be $100mg{\cdot}L^{-1}$ and $30mg{\cdot}L^{-1}$ respectively.