• Journal of Plant Biotechnology
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• v.49 no.1
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• pp.61-73
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• 2022

In this study, we developed a reliable and efficient Agrobacterium-mediated genetic transformation system by applying sonication and vacuum infiltration to six chickpea cultivars (ICCV2, ICCV10, ICCV92944, ICCV37, JAKI9218, and JG11) using embryo axis explants. Wounded explants were precultured for 3 days in shoot induction medium (SIM) before sonication and vacuum infiltration with an Agrobacterium suspension and co-cultivated for 3 days in co-cultivation medium containing 100 µM/l of acetosyringone and 200 mg/l of L-cysteine. Responsive explants with putatively transformed shoots were selected using a gradual increase in kanamycin from 25 mg/l to 100 mg/l in selection medium to eliminate escapes. Results showed optimal transformation efficiency at a bacterial density of 1.0, an optical density at 600 nm wavelength (OD₆₀₀), and an infection duration of 30 min. The presence and stable integration of the β-glucuronidase (gusA) gene into the chickpea genome were confirmed using GUS histochemical assay and polymerase chain reaction. A high transformation efficiency was achieved among the different factors tested using embryo axis explants of cv. JAKI 9218. Of the six chickpea cultivars tested, JAKI9218 showed the highest transformation efficiency of 8.6%, followed by JG11 (7.2%), ICCV92944 (6.8%), ICCV37 (5.4%), ICCV2 (4.8%), and ICCV10 (4.6%). These findings showed that the Agrobacterium-mediated genetic transformation system will help transfer novel candidate genes into chickpea.

• Vacuum Magazine
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• v.3 no.2
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• pp.4-10
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• 2016

Photovoltaic technology has been intensively developed as one of the most powerful renewable energies, replacing a fossil fuel such as coal and petroleum. Every country in the world has emphasized on development of photovoltaic technology and our government has invested heavily in low cost and high efficiency. Korea institute of industrial technology (KITECH) has lastingly constructed PV R&D infra for development of cost-effective and high efficiency solar cells as well as support of commercialization in PV's small and medium enterprises. In this paper, we introduce the next generation PV R&D and infra in KITECH.

• Journal of Korean Vacuum Science & Technology
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• v.2 no.2
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• pp.85-91
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• 1998

The electrochromic behaviors of de-magnetron sputtered tungsten oxide thin films were investigated during coloration and bleach cycles using in situ real-time spectroscopic ellipsometry. Effective medium approximation and least-squares regression analyses were employed to investigate the electrochromic process. The optical properties of the tungsten oxide film were analyzed using the oscillator model and the evolution of the process using a reaction-limited model. In these analyses, we found that two different reaction rates were associated with the process. We ascribe this behavior to the microstructure of this films.

• Tribology and Lubricants
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• v.10 no.3
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• pp.29-38
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• 1994

The friction and wear of mild steel at dry sliding surfaces under different vacuum conditions have been investigated to understand the wear mechanisms. For the test, a ball-ondisk typed wear-rig has been built and implemented, allowing control of sliding speed, load and vacuum. Results show that, at a high sliding velocity, friction of low carbon steel (SS41) under a high vacuum is much higher than that of ambient condition and wear is much severer. It is due to lack of effective oxidation film formation on which steel surfaces could protect themselves against the severe wear. It has been shown, however, that there is a critical regime with contact conditions (at a low sliding velocity, a low load, and under a medium vacuum) at which effective, protective films of low carbon steel have been built on the surfaces in a friction process with a self-regulating way, resulting in both very low coefficients of friction (about 0.3) and mild wear. In order to investigate the protective films on steel surfaces, the worn surfaces and the wear debris have been experimentally analyzed with SEM, AES/SAM and XRD. A theoretical analysis of frictional heating at sliding surfaces, and an experimental analysis of the influence of oxidation wear under various vacuum conditions are described. The important variables on which self-formations of protective films at dry sliding surfaces depend, and the wear mechanisms are also investigated.

• Preventive Nutrition and Food Science
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• v.8 no.2
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• pp.196-199
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• 2003

We investigated the antagonistic effects of two lactococcal bacteriocins, nisin or lacticin NK24, on the growth and the survival of Listeria monocytogenes in vacuum or modified atmosphere-packaged ground beef, Ground beef was inoculated with approximately 3 log colony-forming units (CFU) of L. monocytogenes ATCC 15313 culture per gram of ground beef. Inoculated samples were blended with/without 100 AU/g nisin or lacticin NK24, and subsequently vacuum or modified atmosphere packed at 4$^{\circ}C$. Listeria in the bacteriocin-treated and control samples was subsequently isolated from both vacuum and modified atmosphere packs and enumerated as CFU on Listeria Isolation Agar medium. Microbial counts in ground beef treated with bacteriocin declined steadily, while those of non-treated beef samples increased steadily. The results obtained demonstrate that nisin inhibits the growth of L. monocytogenes more effectively than lacticin NK24 at 100 AU/g. The use of lactococcal bacteriocins, such as nisin or lacticin NK24, in vacuum or modified atmosphere packaging offers a promising approach for eliminating or reducing the risk of L monocytogenes contamination in ground beef.

• 전기의세계
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• v.29 no.8
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• pp.519-523
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• 1980

The traditional mechanical manometer is improved to develop more convenient and precise manometer in continuous measurement of the gas pressure in medium-low vacuum range (1x10$^{-1}$ -10 Torr.) Glass (solid) is used as a detector material of the improved manometer. Using the strain gauge adhered to thin glass board, mechanical strain corresponding to variation of pressure in measurement system is converted into quantity of electricity, and the quantity of electricity is amplified. Experiments have also shown that the improved manometer have more advantages in reproducibility, measured sensibility, and responsible velocity than taditional one.

• Journal of the Korean Ceramic Society
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• v.15 no.2
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• pp.85-88
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• 1978

Influence of cooling atmosphere on the whiteness in white portland cement was studied. The effective composition of gaseous medium of cooling atmosphere is desirable less than 0.5% of $O_2$ and 0.5~1.5% of CO. When theclinker is quenched in nitrogen atmosphere, the color of clinker becomes bright pink. In a weakly reducing gaseous medium or vacuum, the absence of oxygen brings defects in crystal and transformation in the coordination of iron, and as a result, whiteness is raised.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.20-20
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• 2011

It has been known that, under certain conditions, application of low-temperature atmospheric-pressure plasmas can enhance proliferation of cells. In this study, conditions for optimal cell proliferation were examined for various cells relevant for orthopaedic applications. Plasmas used in our experiments were generated by dielectric barrier discharge (DBD) with a helium flow (of approximately 3 litter/min) into ambient air at atmospheric pressure by a 10 kV~20 kHz power supply. Such plasmas were directly applied to a medium, in which cells of interest were cultured. The cells examined in this study were human synoviocytes, rat mesenchymal stem cells derived from bone marrow or adipose tissue, a mouse osteoblastic cell line (MC3T3-E1), a mouse embryonic mesenchymal cell line (C3H-10T1/2), human osteosarcoma cells (HOS), a mouse myoblast cell line (C2C12), and rat Schwann cells. Since cell proliferation can be enhanced even if the cells are not directly exposed to plasmas but cultured in a medium that is pre-treated by plasma application, it is surmised that long-life free radicals generated in the medium by plasma application stimulate cell proliferation if their densities are appropriate. The level of free radical generation in the medium was examined by dROMs tests and correlation between cell proliferation and oxidative stress was observed. Other applications of plasma medicine in orthopaedics, such as plasma modification of artificial bones and wound healing effects by direct plasma application for mouse models, will be also discussed. The work has been done in collaboration with Prof. H. Yoshikawa and his group members at the School of Medicine, Osaka University.

• Journal of Plant Biotechnology
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• v.4 no.4
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• pp.151-154
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• 2002

Conditions for lily pollen growth in vitro and transformation were optimized. Active pollen tube development was achieved effectively in a medium containing 7% sucrose with pH adjusted to 5.7 at the temperature of 27$^{\circ}C$ for about 16-24 hours. Pollen growth was little impaired by the presence of kanamycin at concentration up to 100 mg/L. Pollen rains near the beginning of germination stage were more reliable for Agrobacterium-mediated GUS DNA transformation via vacuum infiltration lasted for 20-40 minutes. GUS DNA integration and its expression in fully developed pollen tubes could be confirmed by Southern blot hybridization, RT-PCR and histochemical staining.

• Journal of Power System Engineering
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• v.17 no.1
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• pp.36-41
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• 2013

A study of the possible axisymmetric modes that propagate at low frequencies in buried, water-filled pipes is presented. It is well known that for a vacuum-pipe-vacuum system the sole non-torsional axisymmetric mode that exists at low frequencies is the fundamental L(0,1) mode. When a pipe is filled with water and still surrounded by a vacuum it is also known that another mode then appears which at low frequencies is characterized by predominantly axial water-borne displacements. In addition to these modes. this paper explores two other, less well known axisymmetric modes whose exitence depends on the acoustic properties of the outer medium that surrounds a pipe. The predicted characteristics of these modes are presented and the likelihood of them propagating over any significant distance in a buried water pipe is discussed.