• CELLMED
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• v.4 no.1
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• pp.5.1-5.8
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• 2014

Healthcare-associated infection represents a frequent cause of mortality that increases hospital costs. Due to increasing microbial resistance to antibiotics, it is necessary to search for alternative therapies. Consequently, novel alternatives for the control of resistant microorganisms have been studied. Among them, plant antimicrobial protein presents enormous potential, with flowers being a new source of antimicrobial molecules. In this work, the antimicrobial activity of protein-rich fractions from flower tissues from 18 different species was evaluated against several human pathogenic bacteria. The results showed that protein-rich fractions of 12 species were able to control bacterial development. Due its broad inhibition spectrum and high antibacterial activity, the protein-rich fraction of Hibiscus rosa-sinensis was subjected to DEAE-Sepharose chromatography, yielding a retained fraction and a non-retained fraction. The retained fraction inhibits 29.5% of Klebsiella pneumoniae growth, and the non-retained fraction showed 31.5% of growth inhibition against the same bacteria. The protein profile of the chromatography fractions was analyzed by using SDS-PAGE, revealing the presence of two major protein bands in the retained fraction, of 20 and 15 kDa. The results indicate that medicinal plants have the biotechnological potential to increase knowledge about antimicrobial protein structure and action mechanisms, assisting in the rational design of antimicrobial compounds for the development of new antibiotic drugs.

• Korean Journal of Ecology and Environment
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• v.43 no.3
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• pp.400-408
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• 2010

The growth and microcystins production characteristics of three species of Microcystis (M. aeruginosa, M. ichthyoblabe, M. viridis) isolated from Yeongchun dam and Ankei dam in Kyungpook Province, South Korea were investigated at temperatures of $15{\sim}35^{\circ}C$ and light intensities of $35{\sim}180\;{\mu}mol\;m^{-2}\;s^{-1}$. All of the three species exhibited the highest growth rates (${\mu}_{max}$) over the $30^{\circ}C$. The maximum growth rates of M. aeruginosa and M. ichthyoblabe was observed at $70\;{\mu}mol\;m^{-2}\;s^{-1}$, while M. viridis showed maximum growth rate at $35\;{\mu}mol\;m^{-2}\;s^{-1}$. The maximum production of total microcystins was observed at $20^{\circ}C$, and the production of microcystins decreased according as temperature increase. The highest microcystins production of M. aeruginosa, M. ichthyoblabe and M. viridis observed at light intensities of $120\;{\mu}mol\;m^{-2}\;s^{-1}$, $70\;{\mu}mol\;m^{-2}\;s^{-1}$ and $35\;{\mu}mol\;m^{-2}\;s^{-1}$, respectively. The concentration of microcyst in production and microcystin types of three species according to temperatures and light intensities showed clear difference between the species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.5
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• pp.425-431
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• 2006

In order to investigate the effects of ethylene on the seed development of three corn types (dent, sweet, and super sweet corns), aminoethoxyvinyl glycine (AVG) and silver thiosulfate (STS) and ethephon (2-chloroethylphosphonic acid, CEPA) were applied either on whole plants or shanks of ears at 9 and 21 days after silking. Ethylene production of developing super sweet corn seeds was much higher than those of sweet and dent corns. The cavity in the endosperm tissues of the super sweet corn started earlier and endosperm was collapsed more severely compared to those of sweet and dent corns. Ethylene production seemed to be related to the death of endosperm cells to form a cavity. Application of AVG and STS reduced ethylene production and delayed cavity formation in endosperm of super sweet corn seeds, while CEPA increased ethylene production and enhanced the time of cavity formation. AVG and STS increased 100-seed weight, while CEPA decreased.

• Journal of Pharmacopuncture
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• v.20 no.2
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• pp.93-99
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• 2017

Objectives: Selaginella bryopteris L. (family: Selaginaceae), is often used in traditional Indian systems of medicine for the prevention and cure of several disorders and for the treatment of patient with spermatorrhoea, venereal disease, constipation, colitis, urinary tract infections, fever, epilepsy, leucorrhoea, beri-beri and cancer. It is also used as a strength tonic. This study aimed to evaluate the mechanisms underlying the anti-inflammatory effects of topically administered aqueous, polar and non-polar methanolic fractions ($10mg/20{\mu}L$) of Selaginella bryopteris. Methods: An acute oral toxicity study of Selaginella bryopteris at doses from 250 to 2,000 mg/kg body weight (bw) was performed. Aqueous, polar and non-polar methanolic extracts ($10mg/20{\mu}L$) applied topically for 5 days were evaluated for their anti-inflammatory effects against 12-tetra-O-decanoyl phorbol acetate (TPA)- induced inflammation by using the redness in the ear, the ear's weight (edema), oxidative stress parameters, such as lipid-peroxide (LPO) and nitric oxide (NO), and the pro-inflammatory cytokines involved in inflammation, such as tumour necrosis factor $(TNF)-{\alpha}$, interleukin $(IL)-1{\beta}$ and IL-6. Indomethacine ($0.5mg/20{\mu}L$) was used for the positive control. Results: Selaginella bryopteris produced no mortalities when administered orally at doses from 250 to 2,000 mg/kg bw. Topical treatment with the non-polar methanolic fraction ($10mg/20{\mu}L$) significantly suppressed redness ($2.4{\pm}0.5$) and edema ($30.4{\pm}1$) and effectively reduced the LPO level ($32.3{\pm}3.3$). The NO level was ($8.07{\pm}0.55$), and the $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6 levels were decreased to $69.6{\pm}15.5$, $7.7{\pm}4.8$ and $82.6{\pm}5.9$, respectively. Conclusion: This study demonstrated for the first time the mechanisms underlying the anti-inflammatory effect of medicinal plants like Selaginella bryopteris and quantified the pharmacological interactions between them. The present study showed this herbal product to be a promising anti-inflammatory phytomedicine for the treatment of patients with inflammatory skin diseases.

• Journal of Oriental Neuropsychiatry
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• v.2 no.1
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• pp.3-18
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• 1991

Serotonin was chemically identified as 5-hydrowytryptamin which occurs in plants, animals, and human beings. The end product of metabolism was excreted as 5-HIAA in urine. Many scientists, specially biochemist and psychiatrist reported that the change of serotonin concentration caused mental disorder and pathological condiions such as schizophrenia and carcinoid. This study was carried out to observe the urinary 5-HIAA excretion rates changed by three recipes treatment (Kamisoyosan, Ondamtang, kalgunhaegitang) according to the classification of endogenous, exogenous and non-exo-endogenous, causes of disease. The urinary 5-HIAA excretion rates before and after three recipes treatment on normal groups divided into three groups on the basis of physical constitutional differences and on patients groups, divided into three groups according to the causal factor were measured. The results were as follows; 1. The urinary 5-HIAA volume of patients groups appeared non-exoendogenous, endogenous, exogenous causes of disease in order of three causative classification. 2. The urinary 5-HIAA volume of Normal groups was $2.50{\pm}088mg/24hrs$(range $0.30{\sim}6.90mg/24hrs$) 3. Thr urinary 5-HIAA volume was $3.70{\pm}0.89mg/24hrs$(range $0.90{\sim}6.50mg/24hrs$) before kamisoyosan treatment and $3.20{\pm}0.72mg/24hrs$ (range $0.80{\sim}6.20mg/24hrs$) after the treatment. 4. The value was $2.60{\pm}0.10mg/24hrs$ (range $0.60{\sim}6.50mg/24hrs$) before Ondamtange treatment and $2.00{\pm}0.12mg/24hrs$(range $0.20{\sim}6.10mg/24hrs$) after the treatment. 5. The value was $4.30{\pm}0.75mg/24hrs$(range $0.92{\sim}6.50mg/24hrs$) before kalgunhaegitang treatment and $3.10{\pm}0.10mg/24hrs$(range $0.80{\sim}5.80mg/24hrs$) after the treatment. Considering the above-mentioned the study on the changes of urinary 5-HIAA volume will make a significant contribution to the diagnosis and the evaluation of therapeutic effect successive research and modified application will be in need.

• Research in Plant Disease
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• v.26 no.3
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• pp.159-169
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• 2020

Pectobacterium carotovorum subsp. carotovorum (Pcc) causes bacterial soft rot on a wide range of crops worldwide, especially in countries with warm and humid climates. This study was conducted to establish an efficient screening method for resistant cultivars of Chinese cabbage to bacterial soft rot. Resistance degrees of 65 commercial Chinese cabbage cultivars to the Pcc KACC 10225 isolate were investigated. For further study, three Chinse cabbage cultivars (Taebong, Hadaejangkun, CR Alchan) showing different level of resistance to the bacterium were selected. The development of bacterial soft rot on the cultivars was tested according to several conditions such as growth stage of Chinse cabbage seedling, inoculum concentration, and incubation temperature after inoculation. On the basis of the results, we suggest that an efficient screening method for resistant Chinses cabbage to Pcc is to inoculate twenty one-day-old seedlings with a bacterial suspension of Pcc at a concentration of 1×10⁷ cfu/ml, and to incubate the plants in a dew chamber at 25℃ for 24 hr and then to cultivate in a growth room at 25℃ and 80% relative humidity with 12-hr light per day.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.3
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• pp.234-238
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• 1989

This study was carried out to find out favorable soil conditions to give good quality of Scutellaria baicalensis George for Korean medicine. The samples were collected from the 7 fields of An-dong gun and Eui-sung gun. The plants were grown under soils of different physicochemical properties and the available constituents from their roots were analyzed by HPLC. The average N, P, K contents in roots was determined to be 0.28-1.26%, 0.17-0.46% and 1.00-1.49%, respectively. The baicalin contents of root was determined to be 11.41-14.76% (An-dong gun) and 13.67-16.31% (Eui-sung gun). The contents of N, P in root were negatively correlated with the contents of exchangeable cations (K, Ca, Mg), clay and organic matter in soil, whereas it was not significant with the contents of total nitrogen and available phosphorus of surface soils. The baicalin contents was positively correlated with the exchangeable potassium contents in soil, but negatively correlated with nitrogen and phosphorus contents in the root of Scutellaria baicalensis George.

• Journal of Food Hygiene and Safety
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• v.19 no.3
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• pp.112-118
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• 2004

It has been reported that various kinds of chinese herbs have an activity of promote growth rate in both animals and human. To investigate the growth promoting effect of the selected plants, fish and pigs were used as experimental animals. In fish, Eleutherococcus senticosus extract and Artemisia capillasis extract were found to be most effective. And these plant extracts were given to pigs. The result showed that plant extracts-fed pigs were significantly increased their body weight gain at 7.06% of average daily gain, as compared to control. The verify this results, secreted growth hormone (GH)levels and insulin-like growth factor-1 (ICF-1) levels into blood were measured. This result indicated that GH and IGF-1 levels in the blood in plant extracts-fed pigs were higher that those of control. To confirm growth promotion effect on human, we manufactured the mixture of these plant extracts, and coated this mixture onto rice, named as $Kiwoomi^{TM}$. When we administered $Kiwoomi^{TM}$ to elementary students, it was found to be effective in growth promotion. This result showed that $Kiwoomi^{TM}$-treated elementary students significantly increased their growth rate (about 2.14 times), as compared to untreated children. Taken together, it is suggested that this functional rice ($Kiwoomi^{TM}$) might be helpful for growing children without any side effects.

• The Korean Journal of Pesticide Science
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• v.13 no.1
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• pp.28-38
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• 2009

An extrapolation of residue data of seven commonly used pesticides namely bifenthrin, chlorothalonil, cypermethrin, diazinon, fenvalerate, phenthoate and procymidone on a total of 22 minor crops has been carried out in an experimental field trial. The pesticides were applied to 11 leafy-, 5 root- and 6 stem-crops grown in the experimental green-house and the crops and plants were randomly collected at 1, 3, 5, 7 days after application. The average recoveries of applied pesticides were ranged from 72.0 to 117.0% in leafy crops, from 81.3 to 105.0% in stem crops and from 70.1 to 108.1% in the root-crops. Limits of detection (LODs) were 0.005-0.1 mg/kg in the leafy crops and 0.001-0.005 mg/kg in both the stem & root crops. Based on the results of residual dissipation pattern and their morphology, all crops were classified into high and low residual groups. The results showed that it might be possible to extrapolate residual data of stem-crops to root-crops within the same group. Crops that have currently no registered pesticide for use, would be possible to use the pesticides which are already been registered for the similar crops.

• Journal of the Korean Professional Engineers Association
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• v.12 no.1
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• pp.12-20
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• 1979

The early history of gun powder (black powder) and explosives was closely connected with the discovery of methods of preparing and purifing salpetre (potassium nitrate KNO$_3$). The Chineses apparently became acquainted with salpetre firstly on about 11th century, and they were possibly the original discoverers of salpetre for raw material of gun powder. The Egyptians called it “Chinese snow”, and it is significant that Chingis-Khan, the Mongol conqueror, took the Chinese eenginees with him in 1218 to use it for attacking the fortifications of the Persian cities. The black powder was invented by chance by Chinese alchemists during the Song dynasty (11th century) in the process of manufacturing medicine, and the powder was introduced to Europe by Mongol army. The manufacturing method of salpetre and gun powder was introduced to Korea from China in 1374, and the powder alld gunnery manufacturing project was developed by Mu Sun Choe(崔茂宣), the first Korean engineer late in Koryo dynasty. Coming in to Yi dynasty the explosive technic, extractive method of salpetre, and gunnery manufacturing process were developed greatly by Mu Sun Choe and Hai Sin Choe (崔海臣). However, confronting with the Japanes invasion at Imjin War (1597) with more powerful western style rifles which had been introduced from the Portuguese, on the contrary Korean army with the traditional guns couldn't compete with them. The Chochong(烏銃, the western rifle introduced in Japane) were much superior to the Chinese style traditional guns in the shooting power and striking efficiency. On the other hand, the Japanese battle ships armed only with the Chochong, when confronted with the Korean turtle shaped ships under the commanding of Admiral Yi Sun-Sin(李舞臣), were defeated by the Korean canons on the ships. The technical development of the modern powder industry in Korea. with the construction of four big explosive plants from 1930 to 1945, has resulted the mass-production of explosives. This study was purposed to investigate to the process with regard to the details of introduction to the explosive technology in Korea, and intended to give a help to the engineers who are engaged in study of the explosive technics by means of giving a spot light data on the early process of the designs, and making suggestion to the researchers for further study and invent a new and modern explosive.