• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.3
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• pp.273-280
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• 1999

Hypocotyl explants of Medicago saliva cv. Vernal were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of growth regulators. After six weeks of culture, somatic embryos were formed from calli on MS medium containing $4mg/{\ell}$ 2,4-D and $0.1mg/{\ell}$ kinetin, or $4mg/{\ell}$ 2,4-D and $0.5mg/{\ell}$ kinetin. The mature somatic embryos were developed to plantlets when subcultured on MS basal medium. In order to obtain secondary somatic embryogenic calli, cotyledon of regenerated plantlets were cultured on a callus induction medium. Embryogenic calli were formed on MS medium containing $4mg/{\ell}$ 2,4-D alone. For induction and development of secondary somatic embryogenesis, the embryogenic calli were transferred to MS basal medium containing either 2,4-D or NAA. Multi-secondary somatic embryogenesis was the most effective on MS basal medium with $0.1mg/{\ell}$ 2,4-D. The rate of secondary somatic embryo formation of regenerated plants was 18 times higher than that of seed grown plants. The mature secondary somatic embryo were germinated into plantlets on MS basal medium after six weeks of culture.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.2
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• pp.245-253
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• 2020

Objective: To develop the fermentation quality and chemical composition of alfalfa (Medicago sativa Lam.) silage, plants were inoculated with different lactic acid bacteria (LAB) strains at field 24 hours before harvest. Methods: The treatment groups were as follow: silage without additive as a control and inoculated with each strains of Lactobacillus brevis (LS-55-2-2), Leuconostoc citerum (L. citerum; L-70-6-1), Lactobacillus bifermentans (L. bifermentans; LS-65-2-1), Lactobacillus plantarum (L. plantarum; LS-3-3) and L. plantarum (LS-72-2). All the silages were stored at 25℃. Parameters such as pH, microorganism and volatile fatty acid contents, crude protein, neutral detergent fiber, acid detergent fiber, net gas, metabolizable energy, organic matter digestibility, dry matter intake and relative feed value were measured to determine fermentation quality, chemical compositions and relative feed value of alfalfa silages. Results: Significant differences were found among the control and treated groups in terms of pH and microorganism contents at all opening times and crude protein, net gas, metabolizable energy and organic matter digestibility of final silage. The pH values ranged from 4.70 to 5.52 for all treatments and control silage had the highest value of overall treatments at T₇₅d silages. Volatile fatty acid of silages was not influenced significantly by inoculations. However, lactic acid content of L. bifermentans (LS-65-2-1) was higher than the other treatments. The highest metabolizable energy and organic matter digestibility were recorded from L. citerum (L-70-6-1) inoculation. In addition, no significant differences were found among treatments in terms of neutral detergent fiber, acid detergent fiber, dry matter intake and relative feed value. Conclusion: Among the treated LAB isolates, L. bifermentans came into prominence especially in terms of organic acid composition and quality characters of silages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.228-235
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• 1997

This experiments were conducted to determine the effect of allelopathy and autotoxicity in alfalfa. Among several alfalfa cultivars, the substances exudated from seven cultivars including Medicago sativa, c.v. Luna, Sparta, Magnum, Husky, Milkmaker, Challenger and Anchor inhibited the germination and seedling growth of radish, ranging from 60 to 80％. When allelopathic substances were exudated from the soil of the alfalfa-cultivated field, the increased levels of substance retarded the germination rate and seedling growth of radish and alfalfa. The inhibition rate was about 80％, compared with that of control. Thus, this study indicates that the allelopathy and autotoxicity substances of alfalfa influenced the germination and seedling growth in alfalfa itself and radish. When allelopathic substances were analysed by using gas chromatograph, several kinds of phenol compounds were detected as follows; Salicylic acid, hydroxybenzoic acid, vanillic acid, syringic acid, coumaric acid, and ferulic acid.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.641-644
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• 2000

In vitro true digestibility of cup-plant (Silphium perfoliatum L.) is higher than other alternative forages and comparative to alfalfa (Medicago sativa L.) even at the high neutral detergent fiber (NDF) concentration. This study was conducted to determine whether the digestion kinetic parameters of cup-plant could explain high in vitro true digestibility of cup-plant at the several NDF levels. Cup-plant and alfalfa were both collected in Arlington and Lancaster, Wisconsin to meet the NDF content within 40 to 50% range. The collected samples were incubated with rumen juice to investigate the digestion kinetics at 3, 6, 9, 14, 20, 28, 36, 48, and 72 h. Kinetics was estimated by the model $R=D_0\;e-k(t-L)+U$ where R is residue remaining at time t, and $D_0$ is digestible fraction, k is digestion rate constant, L is discrete lag time, and U is indigestible fraction. Parameters of the model were estimated by the direct nonlinear least squares (DNLS) method. Digestion rate and potential extent of digestion were not statistically different in either forage. However, alfalfa had shorter lag time (p<0.05). The indigestible fraction increased with maturation in alfalfa and in cup-plant (p<0.05). The ratio of indigestible fraction to acid detergent lignin (ADL) was higher in cup-plant than in alfalfa (p<0.05). From the results, alfalfa is probably digested more rapidly than cup-plant, however, cup-plant maintains higher digestibility with maturation due to a relatively slower increase of indigestible fraction in NDF.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.88-92
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• 1994

This study was carried out to investigate the effects of lime(0, 250, 500 and 1,000 kg/lOa) and phosphate (0, 17 and 34 kg/lOa) applications on growth and nitrogen fixation of alfalfa (Medicago sativa L.). The phosphate applications improved(p<0.05) shoot, root and root nodule dry weight of Alfalfa and acetylene reduction activity of alfalfa with increasing levels of phosphate in the soil pH 6.2 at 7 and 12 weeks(ear1y bloom) after sowing, but these were not influnced with lime applications. Total nitrogen content of each part of alfalfa was increased with lime application, but was not significantly different by levels of lime application at 7 weeks after sowing. Application of phosphate did not affect total nitrogen content of each part of alfalfa. Total nitrogen contents of each part of alfalfa were not significantly different between lime and non-lime application at 12 weeks(ear1y bloom) after sowing but were decreased(p<0.05) with phosphate application(p< 0.05). These results suggest that lime(250 kg/lOa) and phosphate(34 kg/lOa) applications are effective for the growth and the nitrogen fixation of alfalfa at the soil pH 6.20.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.3
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• pp.243-247
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• 2016

Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.

• Plant Biotechnology Reports
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• v.3 no.4
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• pp.301-307
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• 2009

To understand the adaptability of alfalfa (Medicago sativa L.) to chilling stress, we analyzed the antioxidative mechanism during seed germination. The germination rates of six alfalfa cultivars were studied comparatively at $10^{\circ}C$. Xinmu No. 1 and Northstar were selected as chilling stress-tolerant and stress-sensitive cultivars for further characterization. After chilling treatment, Xinmu No. 1 showed higher seedling growth than Northstar. Xinmu No. 1 exhibited low levels of hydrogen peroxide and lipid peroxidation compared with Northstar. In addition, shoots in Xinmu No. 1 treated with chilling showed higher activities of the superoxide dismutase, ascorbate peroxidase (APX), and catalase than those of Northstar, whereas Xinmu No. 1 showed higher APX activity in roots that Northstar. These results indicated that high antioxidation activity in Xinmu No. 1 under chilling stress is well associated with tolerance to chilling condition during germination.

• Korean Journal of Plant Resources
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• v.19 no.3
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• pp.405-410
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• 2006

A series of aqueous or methanol extracts from four different Korean salad plants were assayed to determine their allelopathy and antioxidant activity. The extracts applied on filter paper in a Petri-dish bioassay significantly inhibited root growth of against alfalfa (Medicago sativa) seedlings. Leaf extracts from 40 g dry tissue $L^{-1}$ of Aster yomena was most phytotoxic to alfalfa root growth, and followed by that of Cirsium japonicum, Taraxacum officinale, and Ixeris dentate. Methanol extracts of plants dose-dependently increased DPPH free radical scavenging activity in vitro. Antioxidant activity of methanol extracts from the same plant species was investigated, and the result showed high DPPH free radical scavenging activity in Cirsium japonicum, Aster yomena, and Ixeris dentate, however, in Taraxacum officinale was least activity. By means of HPLC analysis, chlorogenic acid, p-coumaric acid, and total phenolics with 7.68, 17.47 and 18.64 mg, $100g^{-1}$, respectively, showed the highest amounts in methanol extracts from Cirsium japonicum leaves. These results suggest that Compositae salad plants contain water-soluble substances with allelopathic potential as well as antioxidant activity.

• Journal of Animal Science and Technology
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• v.64 no.2
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• pp.262-273
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• 2022

Under the four-cut system, low-lignin alfalfa (Medicago sativa L.) may extend harvesting intervals improving harvest management flexibility and producing forage products with higher nutritive values. The objective of this study was to compare forage yield and nutritive values of low-lignin and conventional alfalfa varieties when applied to six different harvest schedules in the first (2016) and second (2017) production years. There were 12 treatments of two alfalfa varieties as whole plots and six harvest schedules as subplots. Across harvest schedules, there were four cuttings in two production years. Three harvest intervals including "Standard" (high quality, HQ), "Standard+5-day" (medium quality, MQ), and "Standard+10-day" (high yield, HY) were chosen for the first cutting, and 30-day (HQ) and 35-day (HY) for the second cuttings. The third and fourth cuttings in 2016 were timed near final harvest date and in 2017 occurred at 35-day (MQ) and 40-day (HY). Variety by harvest schedule interaction was not significant, but the whole plot and sub-plot effects were significant. Hi-Gest 360 was consistently higher in nutritive value and with a similar yield as Gunner. Harvest schedules did not consistently differ in forage yield and nutritive values. HS-1 ("Standard" + 35-day + Medium Quality + High Yield) with shorter first two cutting intervals provided lower acid detergent fiber (ADF), neutral detergent fiber (NDF), higher relative feed value (RFV), and similar forage yield compared to other schedules. HS-1 had the highest economic incomes when considering RFV and yield among the six different harvest schedules.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.3
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• pp.177-182
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• 2023

Iron (Fe) is a vital element for plants and other organisms, involving in several physiological processes including respiration, chlorophyll biosynthesis, and photosynthesis. Unfortunately, how Fe accumulation regulates in response to light quality has not been well established in plants. Therefore, the aim of the study was to explore the mechanism of Fe homeostasis by light quality. In this study, we found morpho-physiological attributes were significantly improved in response to blue (λmax: 450) compared to white (λmax: 500) and red (λmax: 660) light. The root-shoot length, plant biomass, photosynthesis efficiency (Fv/Fm) and leafgreen (SPAD) significantly declined in response to white and red light. However, these parameters were improved and iron deficiency was substantially alleviated by blue light exposure in alfalfa seedlings. This study might be useful to the forage breeders and farmers for improving alfalfa yield and nutritional benefits.