Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Phytotoxic Effect, DPPH Radical Scavenging Activity and Chlorogenic Acid Level of Methanol Extracts from Aerial Parts of Several Korean Salad Plants

  • Chon Sang-Uk (Research Institute of Bioresources, Callus Co. Ltd., Gwangju Institute of Science and Technology) ;
  • Kim Young-Min (Dongeuinara Co. Ltd., BIC, Dongshin University) ;
  • Kim Dong-Kwan (Jeonnam Agricultural Research and Extension Services) ;
  • Heo Buk-Gu (Senoco Co. Ltd.) ;
  • Cho Ja-Yong (Dept. of Medicinal Resources & Horticulture Development, Namdo Provincial College)
  • Published : 2006.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

A series of aqueous or methanol extracts from four different Korean salad plants were assayed to determine their allelopathy and antioxidant activity. The extracts applied on filter paper in a Petri-dish bioassay significantly inhibited root growth of against alfalfa (Medicago sativa) seedlings. Leaf extracts from 40 g dry tissue $L^{-1}$ of Aster yomena was most phytotoxic to alfalfa root growth, and followed by that of Cirsium japonicum, Taraxacum officinale, and Ixeris dentate. Methanol extracts of plants dose-dependently increased DPPH free radical scavenging activity in vitro. Antioxidant activity of methanol extracts from the same plant species was investigated, and the result showed high DPPH free radical scavenging activity in Cirsium japonicum, Aster yomena, and Ixeris dentate, however, in Taraxacum officinale was least activity. By means of HPLC analysis, chlorogenic acid, p-coumaric acid, and total phenolics with 7.68, 17.47 and 18.64 mg, $100g^{-1}$, respectively, showed the highest amounts in methanol extracts from Cirsium japonicum leaves. These results suggest that Compositae salad plants contain water-soluble substances with allelopathic potential as well as antioxidant activity.

Keywords

References

  1. Banwart, W.L., P.M. Porter, T.C. Granato and J.J. Hassett. 1985. HPLC separation and wavelength area ratios of more than 50 phenolic acids and flavonoids. J. Chem. Ecol. 11: 383-395 https://doi.org/10.1007/BF01411424
  2. Bell, D.B. 1974. The influence of osmotic pressure in tests for allelopathy. Trans. Ill. State Acad. Sci. 67: 312-317
  3. Blosi, M.S. 1958. Antioxidant determinations by use of a stable free radical. Nature 26: 1199-1200
  4. Boyer, J.S. and E.B. Knipling.1965. Isopiestic technique for measuring leaf water potentials with a thermocouple psychrometer. Proc. N.A.S. 54: 1044-1051
  5. Branen, A.L. 1975. Toxicology and biochemistry of butylateed hydroxyanisole and butylated hydroxytoluene. JAOCS 52: 59-63 https://doi.org/10.1007/BF02901825
  6. Chon, S.U., J.H. Coutts and C.J.Nelson. 2004.Osmotic and autotoxic effects of leaf extracts on germination and seedling growth of alfalfa. Agronomy J. 96: 1673-1679 https://doi.org/10.2134/agronj2004.1673
  7. Chon, S.U., Y.M. Kimand J.C. Lee. 2003. Herbicidal Potential and Quantification of Causative Allelochemicals from Several CompositaeWeeds.Weed Res. 43: 444-450 https://doi.org/10.1046/j.0043-1737.2003.00361.x
  8. Chou, C.H. and L.L. Leu. 1992. Allelopathic substances and interactions of Delonix regia (BOJ) Raf. J. Chem. Ecol. 18: 2285-2303 https://doi.org/10.1007/BF00984951
  9. Chung, I.M. and D.A.Miller. 1995. Effect of alfalfa plant and soil extracts on germination and seedling growth. Agron. J. 87: 762-767 https://doi.org/10.2134/agronj1995.00021962008700040025x
  10. Cuvelier, M.E., H. Richard and C. Berset. 1992. Comparison of the antioxidative activity of some acid-phenols: Structure-activity relationship. Biosci. Biotech. Biochem. 56: 324-325 https://doi.org/10.1271/bbb.56.324
  11. Deshpande, S. S., S. K. Sathe and D.K. Salunkhe. 1984. Chemistry and safety of plant polyphenols. In Nutritional and toxicological aspects of food safety. (Freidman,M. Ed.), Plenum: NewYork. pp. 457-495
  12. Frankle, E.N. 1980. Lipid oxidation. A review. Progress in Lipid Research 19: 1-22 https://doi.org/10.1016/0163-7827(80)90006-5
  13. Harborne, J.B. and M. Turner. 1984. Plant chemosystematics. Academic Press, London, UK
  14. Hodgson, J.M. 1968. The nature, ecology and control of Canada thistle. Tech. Bull. No. 1386, Agri. Res. Serve. USDA, USA
  15. Larson, R.A. 1988. The antioxidants of higher plants. Phytochemistry 27: 969-978 https://doi.org/10.1016/0031-9422(88)80254-1
  16. Miller, D.A. 1996. Allelopathy in forage crop systems. Agron. J. 88: 854-859 https://doi.org/10.2134/agronj1996.00021962003600060003x
  17. Molisch, H. 1937. Der Einfluss Einer Pflanze Auf die Andere-Allelopathie. Fischer, Jena, Germany
  18. Naczk, M. and F. Shadhidi. 2004. Extraction and analysis of phenolics in food. Chromatography A. 1054: 95-111 https://doi.org/10.1016/S0021-9673(04)01409-8
  19. Rice, E.L. 1984. Allelopathy. 2nd ed. Academic Press, New York, USA
  20. SAS (Statistical Analysis Systems) Institute. 2000. SAS/STAT user's guide. Version 7. Electronic Version. Cary, NC, USA
  21. Schuler, P. 1990. Natural antioxidants exploited commercially. In Food Antioxidants, ed. B.J. F. Hudson, Elsevier Applied Science, London, pp. 99-191
  22. Shahidi, F. and M.Naczk. 2004. Phenolics in food and nutraceuticals: Sources, applications and health effects. CRC Press. Boca Raton, FL, USA
  23. Singleton,V.L. and J.A. Rossi. 1965.Acolorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16: 144-158
  24. Stachon, W.J. and R.L. Zimdal. 1980. Allelopathic activitity of Canada Thistle (Cirsium arvense) in Colorado. Weed Sci. 28: 83-86
  25. Stich, H.F., M.D. Rosin, C.H. Wu and W.D. Powrie. 1981. A comparative genoxicity study of chlorogenic acid (3-O-caffeoylquinic acid). Mut. Res. 90: 201-212 https://doi.org/10.1016/0165-1218(81)90001-X
  26. Wanasundara, P.K.J.P.D., F. Shahidi and V.K.S. Shukla. 1997. Endogenous antioxidants fromoilseeds and edible oils. Food Rev. International. 13: 225-292 https://doi.org/10.1080/87559129709541106
  27. Wanasundara, U.N. and F. Shahidi. 1994. Canola extracts as an alternative natural antioxidant for canola oil. J. Ame. Oil Chem. Soc. 71: 817-822 https://doi.org/10.1007/BF02540455
  28. Wardle, D.A., K.S. Nicholson and M. Ahmed. 1992. Comparison of osmotic and allelopathic effects of grass leaf extracts on grass seed germination and radicle elongation. Plant Soil. 140: 315-319 https://doi.org/10.1007/BF00010609
  29. Yoshida, T., K.Mori, T.Hatono, T.Okumura, I.Uehara, K.Komagoe, Y. Fujita and T. Okuda, 1989. Studies on inhibition mechanismof antioxidation by tannins and flavonoids.V. Radical-scavenging effects of tannins and related polyphenols on DPPH radical. Chem. Pharm. Bull. 37: 1919-1921 https://doi.org/10.1248/cpb.37.1919