• 한국작물학회:학술대회논문집
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• 한국작물학회 1996년도 추계 학술대회지
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• pp.102-103
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• 1996

• The Korean Journal of Ecology
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• 제27권1호
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• pp.43-47
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• 2004

본 연구는 구리 내성을 가진 식물을 선별하기 위한 기본 자료를 마련하기 위하여 피, 알팔파, 닭의장풀, 해바라기, 옥수수, 어저귀 등 6종 유식물의 토양 내 구리 농도별 생장율 및 구리 제거율 등을 조사하였다. 대조구 및 50, 100, 200, 300 ㎎-CuCl₂/㎏ 의 토양에 각 식물의 종자를 심어 14일 후 수거하여 발아율, 유식물의 생장 및 생체량, 구리 축적 및 제거력 등을 관찰하였다. 구리 농도에 따른 발아율 저해가 나타나지 않은 식물로는 피, 해바라기, 닭의장풀 등이었다. 그러나 해바라기의 경우 300 ㎎-CuCl₂/㎏ 에서 유식물의 뿌리 및 지상부의 생장율이 대조구의 약 7% 수준으로 큰 저해를 받았으며 생체량도 대조구의 약 35%에 불과해 구리에 의한 생체량 저해가 가장 심한 종으로 나타났다. 이에 반해 피와 닭의장풀은 고농도의 구리 오염 토양에서도 생장률이 높아 구리에 대한 내성이 가장 뛰어난 식물로 나타났다. 특히 피의 경우, 토양 내 구리 제거율이 30% 이상이었으며, 토양 내 구리 농도가 증가할수록 축적량이 꾸준히 증가하여 300 ㎎-CuCl₂/㎏ 에서 ㎏당 약 1020㎎의 Cu를 축적하는 것으로 나타나 phytoremediation에 사용될 가능성 있는 종으로 사료된다.

• 한국초지조사료학회지
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• 제13권4호
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• pp.274-277
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• 1993

본 시험은 석회(0, 250, 500, 1,000kg/10a)와 인산(0, 17, 34kg/10a) 시용이 alfalfa(Medicago sativa L.)의 생장 및 질소고정에 미치는 영향을 구명하기 위해 전남대학교 농과대학내 온실에서 pot로 수행되었다. 파종후 9과에 alfalfa의 지상부와 지하부의 건물중 및 아세틸렌 환원력에 있어서 석회 및 인산시용효과가 나타났으며(p<0.05). 파종후 14과(개화초기)에는 alfalfa의 지상부와 지하부의 건물중에 있어서 석회시용 효과는 인정되지 않았으나 인산시용이 증가함에 따라서 건물중은 증가하였다(p<0.01). 또한 석회 및 인산시용이 증가함에 따라서 아세틸렌 환원력은 유의적으로 증가하였다(p<0.05). 파종후 9과에서 전 질소함량은 석회 및 인산 시용구에서 무시용구보다 감소되었으며(p<0.05), 파종후 14과에서는 석회 시용구는 무시용구보다 전 질소함량이 증가되었으나 인산 시용구는 무시용구 보다 낮아지는 경향을 보였다(p<0.05).

• 한국초지조사료학회지
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• 제11권3호
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• pp.145-152
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• 1991

This experiment was carried out to investigate the effects of liming, manure application, and inoculation with Rhizobium on the early growth, nodulation, seedling establishment, winter survival, and forage yield and quality of alfalfa(Medicago sativa L.) at the Experimental Livestock Farm, College of Agriculture, Seoul National University, Suweon in 1985 and 1986. The results obtained are as follows: 1. Seedling vigor and nodule formation of alfalfa at early stage were the best in the treatments of liminginoculation( L1) and liming-manure-inoculation(LM1). 2. Seedling establishment was not significantly affected by the treatments of liming, manure application, nodulation and the combination of these. 3. The LI and LMI treatments showed the highest winter survival of 94.3 and 83.5%, respectively, but the lowest winter su.rvival of 55.9 and 58.5% was found in no treatment(None) and inoculation( I ), respectively. 4. No differences in crude protein(CP1, acid detergent fiber(ADF) and neutral detergent fiber(NDF) contents were found among the treatments. 5. There were significant differences in the total dry matter yield of alfalfa among the treatments. The total dry matter yields of 10,618, 11,830, 11,855 and 13,456 kg/ha from the treatments of L, LM, LI and LMI, respectively, were significantly higher than those of None, M and I treatments. 6. Based on the results of the experiment, it appears that the dry matter yield of alfalfa could be enhanced by the treatments of liming with Rhizobium inoculation and also liming, manure application and Rhizobium inoculation as a practical method.

• 한국초지조사료학회지
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• 제20권4호
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• pp.275-284
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• 2000

To investigate the effect of Mycorrhiza (Glomus intradics) and Rhizobium inoculation on the N, P utilization and the growth of Alfalfa (Medicago sativa L.), four treatments (non-inoculation, control ; Mycorrhiza inoculation, M ; Rhizobiurn inoculation, R and Mycorrhiza and Rhizobium inoculation, M+R) were applied. The associated analyses were carried out on the early vegetative growth stage (DAS 56, 56 days after seeding) and on the early flowering stage (DAS 126). The decreased rate of total N and P content in soil, with advancing plant growth, was relatively higher in the mycorrhiza andor rhizobium treatments than control. The content of available phosphorus in soil at DAS 126 increased by about 40 % in M and M+R treatment compared to control (141.0 mg PzOg'kg DM), while non-significant changes was observed in R treatment. Total N uptake and P uptake in the control at DAS 126 were 33.91 mglplant and 2.42 mdplant, respectively, about 21, 50 and 51 % of increases in total N uptake and 30, 11 and 47 % of increases in total P uptake were estimated in M, R and M+R treatment. Comparing to control, dry matter yield significantly increased by 8, 27 and 28 %, and crude protein yield also by 21, 42 and 39 %, respectively, in M, R and M+R treatment. The present data indicated that mycorrhiza orland rhizobium inoculation improved N, P utilization, and consequently increased the yield of alfalfa. (Key words : Alfalfa, Mycorrhiza, Rhizobium, N and P Utilization, Growth, Yield)

• Asian-Australasian Journal of Animal Sciences
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• 제22권4호
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• pp.520-527
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• 2009

Alfalfa (Medicago sativa L) is a high-quality forage for ruminants and the main stem is the dominant morphological component contributing to the forage nutritive value in mature alfalfa forage. Shearing force, a fracturing property of plant stem, is an important indictor of forage value. The objectives of this study were to investigate the effects of morphological characteristic on shearing force, the relationship between shearing force and chemical composition, and the relationship between shearing force and in situ digestibility of alfalfa stem. The results showed that linear density (weight per unit length of stem) was more important than chemical composition in affecting shearing force. There was a positive relationship between lignin content and shearing force (r = 0.78). Correlations were not found between shearing force and other chemical components such as neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose and hemicelluloses. In situ digestibility (of dry matter and NDF) was related to shearing force. A negative correlation was found between shearing force and dry matter (DM) digestibility (r = -0.70), and there was also a negative correlation between shearing force and NDF digestibility (r = -0.87). When shearing force was standardized for stem diameter or stem linear density, the relationship between shearing force and digestibility was consistent regardless of stem diameter and stem linear density. Shearing force was significantly correlated with lignin content and in situ digestibility (of DM and NDF), and was a more direct indicator for estimating forage nutritive value related to animal performance, so it can be used to predict the forage value of alfalfa.

• Asian-Australasian Journal of Animal Sciences
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• 제27권6호
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• pp.825-831
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• 2014

The aim of this study was to determine the nutritive value of some legume species in salt-affected soils of South-East Anatolian region using chemical composition and in vitro gas production kinetics. In this study, Lotus corniculatus, Trifolium alexandrinum, Medicago sativa were sown and tested in four different locations. A 3 by 4 factorial design with 3 legume species and 4 salt levels (non salty electrical conductivity (EC)<4 dS/m; low salt: 4 dS/m>EC<8 dS/m, medium saline: 8 dS/m>EC<16 dS/m and high salt: 16 dS/m>EC) was used in the study. Results indicated that salinity and plants had no significant effect on ash and ether extract. Dry matter (DM), acid detergent fiber, digestible dry matter, dry matter intake (DMI) were affected by plant, salinity and plant${\times}$salinity interaction. On the other hand neutral detergent fiber, relative feed value (RFV), and DMI were affected by salinity and plant${\times}$salinity interaction. Mineral contents were affected by plant species, salinity and salinity${\times}$plants interactions. In vitro gas production, their kinetics and estimated parameters such as were not affected by salinity whereas the gas production up to 48 h, organic matter digestibility, metabolizable energy (ME), and net energy lactation ($NE_L$) were affected by plant and plant${\times}$salt interaction. Generally RFVs of all species ranged from 120 to 210 and were quite satisfactory in salty conditions. Current results show that the feed value of Medicago sativa is higher compared to Lotus corniculatus and Trifolium alexandrinum.

• 한국초지조사료학회지
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• 제16권2호
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• pp.113-120
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• 1996

The experiment was carried out to investigate the effects of phosphate fertilizer level on growth, crude protein content and nitrogen fixation ability of alfalfa(Medicugo sariva L.) at sowing year. The experimental field was conducted using a randomized block design with three replications according to the phosphate application level (200 and 400kfia) in farm of Kongju National University. Research samples were collected at intervals of 7 days from October 7 to December 9 and nitrogen fixation activity of nodule was measured by using the acetylene reduction method (Hardy et al. 1968). The results obtained are summarized as follows : 1. Number of branches, root length and dry matter weight at phosphate fertilizer level of 400kg in sowing year were more increase than those of 200kfia. The number of nodules was not significantly difference between phosphate fertilizer level, because of low temperature to be enough to nodulation of alfalfa. 2. The crude protein content of leaf, stem and root were not significantly affected by the phosphate fertilizer level. but that of leaf was increased at 400kgha on 72 days after sowing. The crude protein content of each part according to growing stages was increased at 44 days and 72 days after sowing. 3. The dry weight of root was not significantly difference between phosphate fertilizer level from 30 days to 51 days after sowing, but that was increased at phosphate fertilizer level of 400kgha from 51 days afier sowing. 4. The amount of ethylene in the samples taken 2 hours and calculated amount of nitrogen fixation were increased at phosphate fertilizer level of 400kfia from 30 days to 65 days after sowing. but there was vice versa from 58 days after sowing.

• 한국작물학회지
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• 제34권1호
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• pp.48-54
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• 1989

외국에서 도입한 32개의 서로 다른 Alfalfa 품종을 우리나라 기후풍토에서 재배하여 초장과 건물중 등의 생육상태를 비교 검사한 결과 가장 우수한 생육상태를 나타낸 품종들은 다음과 같다. 1. 초장생육이 우수하였던 상위 7개 품종들은 Diamond>Milkmaker> Arrow>Chalienger>Sapphire > Dekalb brand 135> LS-1920의 품종순이고 최하위 초장의 7개 품종을 하위순으로 열거하여 보면 Pioneer brand 5929> Maxidor> Dekalb brand 167> Pierce> Blazer> Raidor> Pioneer brand 532의 순이었다. 2. 건물중이 많은 상위 7개 품종은 Pike>LS-1920>Challenger>Salute>Diamond>Decathlon>Pioneer brand 581의 품종순이며 최하위 건물중의 7개 품종은 Maxidor>Pioneer brand 5929>Pioneer brand 532>Florida 77> Pierce> Magnum> Blazer의 순이었다. 3. 초장과 건물중의 결과를 비교하여 보면 대체로 초장에서 우수하였거나 열거하였던 품종들이 역시 건물중에서도 우세하거나 열거하여 서로가 비례하는 결과를 보였다. 4. 이상에서 조사한 바와 같이 가장 좋은 생육상태 및 수활량을 보인 품종들은 Pike, LS-1920, Challenger, Salute, Diamond 등이었으며 이들중 Challenger 품중을 제외한 나머지 품종들은 내한성이 중간정도인 Semi-dormant 계통으로 우리 나라 재배환경에 가장 적합할 것으로 생각된다.

• 한국초지조사료학회지
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• 제21권1호
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• pp.27-30
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• 2001

알팔파 (Medicago sativa L.)의 줄기부분을 절단하여, SH 배지에 IBA를 첨가한 농도별로 뿌리 유도 정도를 조사한 결과, IBA 농도를 1.0~1.5 mg/$\ell$로 첨가한 처리구에서 뿌리 유도 정도가 70~75%로서 가장 좋았다. 캘러스로부터 shoot를 유도한 다음 줄기를 취하여 처리한 경우에는. 처리 10일째에 1/2 SH-0 배지에서 10개 (50%), SH-0 배지에서 10 (50%), SH-0.5IBA 배지에서 12개 (60%), SH-1.0IBA 배지에서 15개 (75%), SH-1.5IBA 배지에서 15개 (75%), SH-2.0IBA 배지에서 10개 (50%), SH-2.5IBA 배지에서 9개 (45%), SH-3.0IBA 배지에서 8개 (40%)의 뿌리 유도를 나타내었다.