• Title/Summary/Keyword: Media concern

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Rapid and Sensitive Detection of Lettuce Necrotic Yellows Virus and Cucumber Mosaic Virus Infecting Lettuce (Lactuca sativa L.) by Reverse Transcription Loop-Mediated Isothermal Amplification

  • Zhang, Yubao;Xie, Zhongkui;Fletcher, John D;Wang, Yajun;Wang, Ruoyu;Guo, Zhihong;He, Yuhui
    • The Plant Pathology Journal
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    • v.36 no.1
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    • pp.76-86
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    • 2020
  • Cucumber mosaic virus (CMV) is damaging to the growth and quality of lettuce crops in Lanzhou, China. Recently, however, for the first time an isolate of lettuce necrotic yellows virus (LNYV) has been detected in lettuce crops in China, and there is concern that this virus may also pose a threat to lettuce production in China. Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed to detect the nucleoprotein (N) and coat protein (CP) genes of LNYV and CMV, respectively. RT-LAMP amplification products were visually assessed in reaction tubes separately using green fluorescence and gel electrophoresis. The assays successfully detected both viruses in infected plants without cross reactivity recorded from either CMV or LNYV or four other related plant viruses. Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg2+ at 65℃ for LNYV and 60℃ for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. This rapid, specific, and sensitive technique should be more widely applied due to its low cost and minimal equipment requirements.

Regional Image, Reality and Regional Identity Confusion : focusing on the case of Hwaseong Dongtan New Town (지역 이미지, 리얼리티 그리고 지역정체성의 혼란 - 화성 동탄 신도시를 사례로 -)

  • Cho, Il Hwan
    • Journal of the Korean association of regional geographers
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    • v.19 no.4
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    • pp.697-711
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    • 2013
  • Hwaseong serial murders are not now having any significant concern either with Hwaseong City or Dongtan New Town, a region in Hwaseong City, both spatial and temporal manners. However, despite that, people have come to see Hwaseong City with negative ideas as the mass media drove the people to do so. In this study, how films and newspaper articles have created those negative images of Hwaseong City was investigated and afterwards, residents and students living in Dongtan New Town had interviews on what they think about the negative regional images. The study, then, worked on a mental map and others based on what it learned from the interviews. Regional identity confusion is now being discovered out of the people's resistance against the negative images of the city and through such research process described before, the study discussed this regional identity confusion caused by living as residents in Hwaseong and living as residents in Dongtan New Town. In addition, the study revealed sense of place of Dongtan New Town and aspects of the regional identity confusion with this Dongtan New Town growing both with symbolic meanings as a place of murders, an awful crime, and spatial meanings as another place of dynamic developments and improvements.

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Digital Restoration of Cultural Properties Based on Virtual Reality (가상현실 기술을 이용한 문화재의 디지털 복원)

  • 박소연;양종열
    • Archives of design research
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    • v.16 no.1
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    • pp.219-228
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    • 2003
  • Nowadays, the progress of high technology in modern society provides humankind with the opportunity to obtain diverse information easily and quickly. Among those rapidly progressing fields of high technology, digital media field has the ability to substitute for the multimedia information society by being applied to many related fields and daily life. Specifically, the concern and enthusiasm for the restoration of cultural property has risen. So, effective methods are required to decide on the preservation, restoration and academic value of cultural property. Among these, image processing technology using computers has been introduced. The restoration of cultural property using this technique has been studied vigorously in many advanced countries because this would allow us to restore damaged parts without destroying the original asset. However, in Korea, though we have numerous examples of cultural property with recorded history, research related to the items has scarcely been conducted. Accordingly, this study focuses on the investigation of virtual reality and the techniques applicable to restoring cultural property in addition to suggesting the significance and possibility of digitally restoring cultural property following the introduction of applicable examples both domestically and abroad.

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Design and Implementation of the Traffic and Travel Information Service for Terrestrial DMB System (T-DMB에서의 교통여행정보서비스 설계 및 구현)

  • Kwon, Dae-Bok;Chae, Young-Seok
    • Journal of Broadcast Engineering
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    • v.12 no.3
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    • pp.203-213
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    • 2007
  • DMB video/audio service was successfully launched. DMB data service is a matter of primary concern now. The TTI(Traffic and Travel Information) service is considered as a killer application of the data service. Tn this paper, we propose a method for design and implementation of the broadcasting system for TTI service. The proposed TTI service system consists of an authoring tool, the DB module, the Transmission module and Monitoring module. This system satisfies the national TTA standards of the Traffic and Travel Information data services for terrestrial DMB; TPEG(Transport Protocol Experts Group) platform. The system was designed to support real-time automatic transmission for CTT and CTT SUM, and non real-time authoring & automatic transmission for POI and SDI, and was focused on the capability to make high-quality contents efficiently and to send them to the data inserter reliably. The performance of the implemented system was proven through the conformance tests with the various commercial receivers. After the continuous upgrade, the system is being used in commercial service.

A study on determinants of flow status in smoking simulation game (흡연시뮬레이션 게임에서 몰입 결정요인에 대한 연구)

  • Jang, Han-Jin;Noh, Ghee-Young
    • Journal of Korea Game Society
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    • v.17 no.2
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    • pp.165-174
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    • 2017
  • This research was conducted to find out determinant variables which can influence the degree of flow in smoking simulation game. We set up flow experience as a dependent variable. Several factors including empathy, health concern, and brainwave(${\alpha}$, ${\beta}$) EEG(Electroencephalogram) as a physiological indicator, which was expected to affect flow, were set up as independent variables to perform regression analysis. As a result, empathy, health consciousness, and ${\beta}$ wave had positive influences on flow in smoking simulation game but ${\alpha}$ wave had negative effect. This research empirically analyzed users' experience and neurophysiological changes which can influence on flow in serious game and contributed to theoretical and methodological development in health-related game research.

Teacher's acceptance of digital games and related factors (학교 장면에서 디지털 게임 이용에 대한 교사의 수용도와 이에 영향을 미치는 요인)

  • Kim, Jee Yeon;Doh, Young Yim
    • Journal of Korea Game Society
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    • v.17 no.2
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    • pp.123-134
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    • 2017
  • The purpose of this study is to investigate how teachers' acceptance of digital games is constructed in the school context and what factors influence teachers' acceptance. To do this, we conducted a survey on teachers, school counselors, and professional counselors in August 2016. A total of 250 data were analyzed. As a result, it was confirmed that the teachers' acceptance of digital game is composed of 5 factors - need for supervision, willingness to use, concern & monitoring, acceptance as alternative activities, acceptance efficacy. As a result of multi-variate multiple regression analysis, it was found that the teacher's age, the 2 factors of digital media literacy, the teacher's attitude toward student's digital game usage, and teacher's evaluation of the impact of digital games on school achievement have different effects on the 5 factors of teachers' acceptance. This study is meaningful to identify how teachers' acceptance of digital games is constructed and to explore the main factors affecting teachers' acceptance of digital games.

Cancer News Coverage in Korean Newspapers: An Analytic Study in Terms of Cancer Awareness

  • Min, Hye Sook;Yun, E Hwa;Park, Jinsil;Kim, Young Ae
    • Journal of Preventive Medicine and Public Health
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    • v.53 no.2
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    • pp.126-134
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    • 2020
  • Objectives: Cancer diagnoses have a tremendous impact on individuals and communities, drawing intense public concern. The objective of the current research was to examine news coverage and content related to cancer-related issues in Korean newspapers. Methods: Primarily using the database system of the Korea Press Foundation, we conducted a content analysis of 2806 articles from 9 Korean daily newspapers during a recent 3-year period from 2015 to 2017. Thematic categories, the types of articles, attitudes and tone, and the number of sources in each article were coded and classified. Results: Many news articles dealt with a diverse range of themes related to cancer, including general healthcare information, the latest research and development, specific medical institutions and personnel, and technology and products, which jointly accounted for 74.8% of all articles. Those thematic categories differed markedly in terms of article type, tone, and the number of cited sources. News articles provided extensive information about healthcare resources, and many articles seemed to contain advertising content. However, the content related to complex social issues such as National Health Insurance did not include enough information for the reader to contextualize the issues properly or present the issues systematically. Conclusions: It can be assumed that the media exert differential influence on individuals through news coverage. Within the present reporting framework, the availability and usefulness of information are likely to depend solely on individuals' capabilities, such as financial and health literacy; this dependency has a negative impact on knowledge gaps and health inequities.

A Legal & Institutional Supporting on Local Broadcast Development - Syntactic Approach about Special Act on the Development of Local Broadcasting (지역방송발전을 위한 법적·제도적 지원방안 -지역방송 발전지원 특별법의 성과와 한계를 중심으로)

  • Kang, Chul-Soo;Kim, Dug-Mo
    • Journal of Digital Convergence
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    • v.15 no.1
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    • pp.45-52
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    • 2017
  • According to the change in media environment, a support plan for local broadcast has been required. In terms of legal and institutional support for the development of local broadcast, the enactment of special Act for local broadcast has become a breakthrough. In this point of view, we analyzed the performance and the limitation of the special Act. As a literature research, analyzing the literature and related-ordinances, we have sought legal and institutional support plans for the local broadcast. As a result, with the Local Broadcast Development Council being in charge of legislative right and responsibility, a plan to empower them to have legal and institutional authority should be drawn; another plan to secure independence in operating a fund and to promote the capability to produce local broadcast programs and the distribution system should be drawn; the other elaborate plan for the locality index that assesses the locality is required. Local broadcast should be on a way to secure not only public interest and concern but locality and diversity; legal and institutional plans should be sought.

Analysis on Awareness and Information Acquisition Behavior Regarding Food Hygiene and Safety of University Students in Shandong Province, China (중국 산동성 지역 대학생의 식품 위생.안전성에 대한 인식도 및 정보 획득 행동 분석)

  • Sun, Xiaoqing;Kim, Hyochung;Kim, Meera
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.2
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    • pp.153-170
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    • 2013
  • This study examined awareness and information acquisition behavior regarding food hygiene and safety of university students to improve safe dietary practices and to get basic information to develop educational materials for food safety. It was conducted among 276 university students of Qingdao University and Liaocheng University in Shandong province, China through a self-administered questionnaire. Data were analyzed by SPSS Windows V.19.0. To describe characteristics of the respondents, frequency distributions were used. In addition, t test, one-way analysis of variance and Duncan's multiple range tests were conducted. The results were as follows: The respondents were generally interested in food hygiene and safety. However, the level of awareness regarding the food hygiene and safety system in China was not high. Almost forty-five percent of the respondents did not think that foods produced and distributed in China were safe. For the reasons for their distrust, most of the students replied that they did not trust the safety 'because of distrust for hygiene of the food process', followed by 'because of distrust for food distribution' and 'because of excess or illegal use of food additives on food process'. The respondents answered for the concern level toward food hygiene and safety foodborne disease threatened food safety the most, followed by heavy metal contamination and endocrine disruptors. The respondents acquired most information from electronic media including TV and radio. Most of the students wanted to participate in food hygiene and safety education. Finally, when they found hazardous or foreign materials in food the most frequent reaction was 'paying attention in the next purchase', followed by 'notifying others', and 'letting it go this time but never buying that product in the future'.

Sensitivity and Rejection Capability of Thermal Asperity Induced by Sub-Micron Contamination Particles (미세 입자에 의한 thermal asperity의 민감도 해석 및 감소 방안)

  • 좌성훈
    • Journal of the Korean Magnetics Society
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    • v.10 no.6
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    • pp.310-317
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    • 2000
  • With use of (G)MR head, thermal asperity (TA) has been a big concern in drive industry. In this study, we investigated several factors of heads and disks which affects the TA sensitivity of the drive. TA experiments were conducted by introducing the particles on the drives using a particle injection chamber. It was found that the slider ABS shape can help to reduce TA or contamination in the head/media interface. However, TA sensitivity of the drive mainly depend on the intrinsic property of (G)MR sensor. GMR head is much less sensitive to TA compared with MR head. However, in case that the same bias current was applied for both of MR and GMR head, TA sensitivity of GMR head became almost identical to that of MR head. Therefore it was found that the bias current is a dominant factor in determining TA sensitivity of the head. TA sensitivity of different types of disks was also studied. The scratch resistance of the carbon overcoat layer is the one of the main factors which influence TA rejection capability of the disks.

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