• Title/Summary/Keyword: Media composition

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Optimization of Media Composition and Culture Conditions for the Mycelial Growth of Coriolus versicolor and Lentinus edodes (Coriolus versicolor와 Lentinus erodes의 영양배지 조성 및 배양조건의 최적화)

  • 박경숙;이재성
    • KSBB Journal
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    • v.6 no.1
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    • pp.91-98
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    • 1991
  • The media compositions and culture conditions were optimized for mycelial growth of Coriolus versicolor and Lentinus edodes. Media composition for optimal growth of Coriolus versicolor was 2.0% glucose 0.4% peptone and 0.6% yeast extract. Media composition for optimal growth of Lenttnus edodes was 2.0% glucose 2.0% starch 0.4% bacto-soytone and 0.6% yeast extract. The media supplemented with KH2PO4, 0.046% KH2PO4 0.1% and MgSO4, .7H2O 0.05% supported better mycelial growth than the media without mineral salts. The optimum temperature for mycelial growth ranged from $25^{\circ}C$-28$^{\circ}C$. The optimum pH range for mycelial growth of Coriolus versicolor was 5.2~5.6 while that of Lentinus edodes appeared to be 5.75.

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Scene Composition Technology Based on HTML5 in Hybrid Broadcasting Environment (하이브리드 방송 환경 하에서 HTML5 기반 장면구성 기술)

  • Jo, Minwoo;Park, Jungwook;Kim, Kyuheon
    • Journal of Broadcast Engineering
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    • v.18 no.2
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    • pp.237-248
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    • 2013
  • Hybrid broadcasting environment is convergence of broadcasting and communication environment. In hybrid broadcasting environment, a number of media can be delivered using both broadcasting channel and other network unlike traditional broadcast environment that is able to deliver a couple of media by the limited bandwidth. Now, starting with smart TV, hybrid broadcasting environment combining broadcasting channel and IP network is established, and a variety of services are appearing. Moreover, the services using hybrid broadcasting environment are expected to appear soon for the other smart terminals such as smart phone and tablet PC. Scene composition is one of the methods that can consume effectively a number of media delivered from hybrid broadcasting environment. Using scene composition, multiple media can be consumed through the specified presentation time and space. Therefore, in this paper, it proposes the scene composition technology that is suitable for hybrid broadcasting environment and smart terminals. However, the spatial composition and temporal composition of media using script language and style language of HTML5 might increase the complexity of processing, and cause limitation of avaliable terminals. Also, a document of HTML5 can describe only one scene. By these reason, the proposed scene composition technology extends HTML5 in order to provide the spatial and temporal composition of media and description of multiple scene through markup language. In addition, it includes the extension of HTML5 in terms of utilization in hybrid broadcasting environment. For this proposal, this paper describes the technology of HTML5 and proposed scene composition. Also, it verifies the scene composition with both implementations and experiments.

Intermediate Data Structure for MPEG-4 Scene Description

  • Cha, Kyung-Ae;Kim, Hee-Sun;Kim, Sang-Wook
    • Proceedings of the Korea Multimedia Society Conference
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    • 2001.06a
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    • pp.192-195
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    • 2001
  • MPEG-4 content is streaming media that are composed of different types of media objects, organized in a hierarchical fashion. This paper proposes scene composition model for authoring MPEG-4 contents which can support object based interactions. And we have developed MPEG-4 contents authoring tool applied the proposed scene composition model as intermediate data structure. Particularly, for supporting interoperability between multimedia contents, the scene composition model should be used independent of file format. So visual scene composed of media objects on the from of scene composition tree can be transformed variable data format including BIFS, scene description from proposed by MPEG-4 standard and also support the extension of capability.

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The optimum media size and composition for sawdust culture of Lentinula edodes (Strain: JNM3022) (표고버섯(JNM3022) 톱밥재배에 적합한 배지조성과 배지크기)

  • Choi, Duck Soo;Jung, Kyung Ju
    • Journal of Mushroom
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    • v.6 no.3_4
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    • pp.131-137
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    • 2008
  • These experiments were conducted to search for the optimum media size and composition for culture of Lentinula edodes (JNM3022). Considering the incubation period and the successful culturing rate, the optimum media size was length 25 $cm{\times}diameter$ 14 cm (weight 2.5 kg). The saturation moisture amount of Oak sawdust was 251 ml/100 g. It is a half of pine sawdust 495 ml/100 g. Considering the hypha growing speed and hypha density, the optimum media composition was Oak sawdust 80%+ wheat bran 20% and Oak sawdust 80%+ rice bran 20% In 10 treatments of media composition. The optimum moisture amount of media for growing Lentinula edodes (JNM3022) was 55%. Injection pipe with 20 holes in media was reduced the incubation periods to 11 days when 20cm length media and 32 days when 30cm length media compare to control. The best method for browning media surface was change temperature condition above $10^{\circ}C$ and punching the vinyl bag. This treat could shortened the browning periods to 54 days.

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Effect of Drainpipe Height and Media Composition on Growth and Yield of Soilless Cultivated Cut Rose in Container Culture (절화장미 용기재배 시 배수구 높이, 배지조성이 생육과 수량에 미치는 영향)

  • Choi, Gyeong-Lee;Cho, Myeong-Whan;Cheong, Jae-Woan;Roh, Mi-Young;Rhee, Han-Cheol
    • Journal of Bio-Environment Control
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    • v.19 no.4
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    • pp.240-245
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    • 2010
  • The objective of this study was to determine the effect of different height of drainpipe and growing media soilless cultivated cut rose in container culture. Two experiment were to examine the effect of the drainpipe height and media composition on yield and quality of cut rose, Four different drainpipe height (0, 3, 6, 9 cm) were treated to determine of optimal container type. Yield was the highest at 3 cm drainpipe height, but quality was not significantly affected by drainpipe height. Survival rate of rose was 100%, 100%, 92%, and 92%, respectively. Two different drainpipe height (0, 3 cm) and 7 media composition (pure coir and pelite, and mixed with two media 3 : 1, 2 : 1, 1 : 1, 1 : 2, 1 : 3 v/v) was treated to determine of media composition related to drainpipe height. The supply of nutrient solution was controlled by the signal of water potential at -5 kPa using frequency domain reflectometry (FDR) sensor in mixed coir with pelite 3 : 1, 1 : 1, 1 : 3 (v/v), respectively. Irrigation frequency reduced in high ratio of coir media and 3 cm height of drainpipe. Quality of cut rose except for flower weight and yield until 2nd harvest was not significantly affected by drainpipe height, but yield after 3rd was higher at 3 cm than 0 cm height of darinpipe. In the media composition, yield and qulity of cut rose was increased at high ratio of coir media.

Effects of the Nitrate and Phosphate Starvation on the Biosynthesis of Phospholipid and the Composition of Fatty Acids in Chlorella Chloroplasts (Chlorella 엽록체의 인지질 생합성 및 지방산조성에 미치는 Nitrate와 Phosphate 결핍효과)

  • 이점규
    • Journal of Plant Biology
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    • v.31 no.3
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    • pp.187-196
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    • 1988
  • Chlorella ellipsoidea were cultured in "cold" media starvated with the nitrate and phsophate sources. The effects of the nitrate and phsophate starvation on the biosynthesis of phospholipid and the composition of fatty acids in chloroplasts isolated from these cells were analyzed. The syntheses of phosphatidylcholine and phosphatidylinositol in the nitrate and phosphate starvation were similarly inhibited as compared with the control but phsophatidylethanolamine synthesis in the nitrate starvation was extremely lower than that in the phosphate starvation. The major fatty acids utilized in phospholipid formation within chloroplasts were palmitic acid and linolenic aicd. However, palmitic acid and stearic acid were dominant in the condition of the nitrate starvation. The levels of palmitic acid were enhanced 3-fold than that of the control. These results suggest that the biosynthesis of phospholipid and the composition of fatty acids were affected by the nitrate and phosphate starvation in the culture media.ure media.

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Doxorubicin Productivity Improvement by the Recombinant Streptomyces peucetius with High-Copy Regulatory Genes Cultured in the Optimized Media Composition

  • PARK, HEE-SEOP;KANG, SEUNG-HOON;PARK, HYUN-JOO;KIM, EUNG-SOO
    • Journal of Microbiology and Biotechnology
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    • v.15 no.1
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    • pp.66-71
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    • 2005
  • Doxorubicin is a clinically important anticancer polyketide compound that is typically produced by Streptomyces peucetius var. caesius. To improve doxorubicin productivity by S. peucetius, a doxorubicin pathway-specific regulatory gene, dnrI, was cloned into a high-copy-number plasmid containing a catechol promoter system. The S. peucetius containing the recombinant plasmid exhibited approximately 9.5-fold higher doxorubicin productivity compared with the wild-type S. peucetius. The doxorubicin productivity by this recombinant S. peucetius strain was further improved through the optimization of culture media composition. Based on the Fractional Factorial Design (FFD), cornstarch, $K_2HPO_4$, and $MgSO_4$ were identified to be the key factors influencing doxorubicin productivity. The Response Surface Method (RSM) results based on 20 independent culture conditions with varying amounts of key factors predicted the highest theoretical doxorubicin productivity of 11.1 mg/l with corn starch of 46.33 g/l, $K_2HPO_4$ of 4.63 g/l, and $MgSO_4$ of 9.26 g/l. The doxorubicin productivity of the recombinant S. peucetius strain with the RSM-based optimized culture condition was experimentally verified to be 11.46 mg/l, which was approximately 30.8-fold higher productivity compared with the wild-type S. peucetius without culture media optimization.

The Optimal Composing the Filter-Bed for Coping with Daily Flow-Rate Fluctuation for the Performance of Sand Filtration Process (일유량 변동에 따른 여과수질 악화에 대처하기 위한 최적 여재구성에 관한 연구)

  • Park, Dong-Hak;Park, No-Suk;Kim, Seong-Su;Bae, Cheol-Ho;Jung, Nahm-Chung;Cjoi, Seung-II
    • Journal of Korean Society of Water and Wastewater
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    • v.21 no.4
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    • pp.485-491
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    • 2007
  • The fluctuation of inlet flow to a water treatment plant makes a serious problem that it can change the filtration rate abruptly, and ultimately occur the breakthrough of the detained particles inside filter media. Also, since it takes very short time (about 10 minutes) for the surface wave occurred from the fluctuation of inlet flow to reach the filtration process, it is impossible to control the filtration rate stably. Therefore, this study was conducted to evaluate the effect of daily flow-rate fluctuation on the performance of sand filtration process, and to suggest the dual media composition for coping with that effect. Comparative column tests have been carried out for various dual media (sand and anthracite) compositions. From the results of column tests, dual media, especially in the case of sand 45cm/anthracite 30cm, is more effective to cope with the effect of flow-rate fluctuation on the performance of filtration than single media (only sand). In addition, irrespective of dual media composition, managing ability to cope with that fluctuation tends to be weak at the end of allowable filtration duration time,

Volatiles Composition from Aerial Parts of the Insect-Pollinated and the Promising Medicinal Plant Spiraea hypericifolia L. Growing Wild in Northern Kazakhstan

  • Kirillov, Vitaliy;Stikhareva, Tamara;Atazhanova, Gayane;Ercisli, Sezai;Makubayeva, Aigerim;Krekova, Yana;Rakhimzhanov, Alimzhan;Adekenov, Sergazy
    • Natural Product Sciences
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    • v.27 no.1
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    • pp.36-44
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    • 2021
  • The essential oils from the aerial parts (leaves and flowers) of Spiraea hypericifolia L. (Rosaceae), collected in Northern Kazakhstan, were obtained by distillation in two dispersion media (distilled water and 15% NaCl solution). The chemical composition of the essential oils was evaluated by GC-MS for the first time. The yield of the essential oil was 0.04% (in fresh growth conditions) and 0.02% (in dry growth conditions) respectively regardless of which dispersion media (H2O or 15% NaCl solution) was used at the isolation of essential oil. The main compounds were aliphatic hydrocarbons (alkanes) (40.6-53.2%), aldehydes (8.4-17.4%), diterpenoids (9.1-16.7%) and ketones (6.2-8.7%). Content of monoterpenoids was depended on dispersion media (2.2-3.6% where H2O was dispersion media and 8.4-8.5% where 15% NaCl solution was dispersion media). n-Heneicosane (17.4-34.1%) and n-tricosane (14.3-19.5%) were the main constituents of the essential oil of S. hypericifolia. There were many insects from different classes in S. hypericifolia at flowering. Important components such as α-methylene-γ-butyrolactone (0.8-2.8%), benzyl cyanide (0.7-1.1%), β-damascenone (1.2-2.9%), (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (1.8-2.7%), β-ionone (0.5-1.8%) and others were detected in small amounts.

Optimization of Media Composition and Cultivation for the Mycelial Growth of Agrocybe Cylindracea (Agrocybe Cylindracea의 영양배지 조성 및 배양조건의 최적화)

  • Lee, Jae-Sung;Park, Shin;Park, Gyung-Sook
    • Korean Journal of Food Science and Technology
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    • v.21 no.3
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    • pp.399-403
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    • 1989
  • Studies were made to optimize the meida composition and cultural conditions for the mycelial growth of Agrocybe cylindracea. Media composition for optimal growth was found to be starch 20.0g/l, bacto-soytone 4.0g/l. The media supplemented with $KH_2PO_4\;0.46g/l,\;K_2HPO_4\;1.0g/l,\;MgSO_47H_2O\;0.5g/l$ supported mycelial growth better than the media without mineral salts. Optimum temperature and pH for the growth was $28^{\circ}C$, and 6.0 respectively. Temperature range for the mycelial growth appeared to be $10-35^{\circ}C$ and the mycelium evidently lost the vitality at $40^{\circ}C$.

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