• The Journal of the Korea Contents Association
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• v.14 no.12
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• pp.694-705
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• 2014

Recently media use is in the period of upheaval because of the advent of smart media. At this present, there are many discussions about the multiplexing or convergence of media use and these effects of everyday life, based on the theory of 'media repertoires'. But many researches just divide media repertoire categories and show media use hours by these categories. To have more mighty power of explanation, the study about media repertoires must execute both a static analysis of media use hours and a dynamic analysis of media use by media repertoire categories in everyday life.

• Korean journal of communication and information
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• v.49
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• pp.20-38
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• 2010

This paper was attempting to identify determinants of media repertoires based on new services and technologies. Using the regression and discriminant models, this study examined determinants that included five independent factors such as the degree of innovation, social networks, social influences, demographic variables and media uses. The analyses revealed that all of independent variables except the degree of innovation were significant determinants of media repertoires. Secondly, the results of discriminant analyses showed that terrestrial television use, age, disposable income were significant factors discriminating new media service adopters from the sample. For new media related technologies adopters, family income, and media uses such as newspaper, Internet and radio were significant discriminant variables.

• Proceedings of the Korea Contents Association Conference
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• 2011.05a
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• pp.281-282
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• 2011

다양한 미디어가 공존하는 가운데 사용자의 미디어사용방식이 다양해지고 있다. 본 연구에서는 관광객이 여행전에, 여행하는 중에 어떤 관광정보미디어를 이용했는지 유형별로 살펴보고, 그 유형을 인구통계학적 변인과 관광객의 여행특성 변인을 통해 설명하고자 한다.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.408-420
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• 2014

Yeast surface-displayed antibody libraries provide an efficient and quantitative screening resource for given antigens, but suffer from typically modest library sizes owing to low yeast transformation efficiency. Yeast mating is an attractive method for overcoming the limit of yeast transformation to construct a large, combinatorial antibody library, but the optimal conditions have not been reported. Here, we report a large synthetic human Fab (antigen binding fragment) yeast surface-displayed library generated by stepwise optimization of yeast mating conditions. We first constructed HC (heavy chain) and LC (light chain) libraries, where all of the six CDRs (complementarity-determining regions) of the variable domains were diversified mimicking the human germline antibody repertoires by degenerate codons, onto single frameworks of VH3-23 and $V{\kappa}1$-16 germline sequences, in two haploid cells of opposite mating types. Yeast mating conditions were optimized in the order of cell density, media pH, and cell growth phase, yielding a mating efficiency of ~58% between the two haploid cells carrying HC and LC libraries. We constructed two combinatorial Fab libraries with CDR-H3 of 9 or 11 residues in length with colony diversities of more than $10^9$ by one round of yeast mating between the two haploid HC and LC libraries, with modest diversity sizes of ${\sim}10^7$. The synthetic human Fab yeast-displayed libraries exhibited relative amino acid compositions in each position of the six CDRs that were very similar to those of the designed repertoires, suggesting that they are a promising source for human Fab antibody screening.