• Archives of Pharmacal Research
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• v.20 no.4
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• pp.297-305
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• 1997

A new serum-free defined medium was developed that supports the growth of normal rat mammary epithelial cells. Mammary organoids from the glands of female F344 rats were cultured in a serum-free medium. Monolayer culture colonies developed within a week and remained viable for months in culture. Upon subculture of one-week-old primary colonies, almost the same morphology of colonies was developed. The scrape loading/dye transfer technique showed that most of colonies that developed in a serum-free medium containing EGF, human transferrin, insulin, and hydrocortisone (basal serum-free medium, BSFM) failed to show cell-cell communication. However, colonies cultured in BSFM supplemented with prolactin, $E_2$, and progesterone (complete hormone serum-free medium, CHSFM) showed cell-cell communication at 14 days of primary culture or of subculture. By flow cytometry with FITCPNA and PE-anti-Thy-1.1 monoclonal antibody, we distinguished four RMEC subpopulations in cultures in both media: Thy-1.1+ cells, PNA+ cells, cells negative to both reagents and cells positive to both reagents. It is likely that combined prolactin, cortisol, and insulin in CHSFM stimulate terminal differentiation of clonogenic cells.

• Nuclear Engineering and Technology
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• v.6 no.4
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• pp.231-237
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• 1974

For detection and localization of leaks in closed vessels or pipes carrying liquid flow, $^{51}$ Cr-EDTA and $^{51}$ Cr-DTPA were synthesized and, the column and batch equilibrium experiments were carried out. In the column experiment, the recovery of $^{51}$ Cr-EDTA is 100% in quartz sand and 80.9% in steel sawdust, and that of $^{51}$ Cr-DTPA is 77.4% in quartz sand and 6.4% in steel sawdust. The recovery curve. $^{51}$ Cr-DTPA system in steel sawdust does not show a certain plateau, exceptionally. In general, $^{51}$ Cr-EDTA is adsorbed less than $^{51}$ Cr-DTPA. In the batch equilibrium experiment, the distribution coefficients (Kd) and effect of pH were investigated by using quartz sand, montmorillonite, steel sawdust, and mixed cement raw material as media. In general, the Kd values for $^{51}$ Cr-EDTA are lower than that of $^{51}$ Cr-DTPA. The Kd values for $^{51}$ Cr-EDTA are almost zero at pH 6.0, 7.0, and 8.0.

• Journal of Korean Society of Transportation
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• v.29 no.3
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• pp.49-58
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• 2011

As a part of the ITS, the traffic information system is more widely adopted to deliver traffic information to vehicle drivers via various types of media. As the ITS system establishment has been recognized as somewhat accomplished, the focus has shifted to the efficient operation and maintenance of the system. In this paper, we propose a method for evaluating a VMS traffic information service system from a user's perspective. In particular, relational expressions for the level of information provided and the level of user satisfaction are generated for the evaluation to take place from the user psychological satisfaction aspect of all other beneficial features of the system. As continuous flow facility is the one that has limited entry and exit, the traffic information of VMS plays a significant role for vehicle drivers to make their decisions. A method suggested in this paper could be used for evaluating an existing system as well as setting up the target service level of the system of a new system. If individual system properties are considered along with various VMS functions being as supplement of this research, more systematic evaluation method could be arranged.

• Journal of the Korean Geotechnical Society
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• v.29 no.8
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• pp.65-73
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• 2013

Ground-coupled heat pump system has attracted attention as a promising renewable energy technology due to its improving energy efficiency and eco-friendly mechanism for space cooling and heating. Pipes buried in the ground play a role of direct thermal interaction between circulating fluid inside the pipe and surrounding soils in the geothermal exchange system. However, both complexities of turbulent flow coupling thermal-hydraulic phenomena and very long aspect ratio of the pipe make it difficult to model the heat exchange system directly. Energy balance for fluid flow inside the pipe was derived to model thermal-hydraulic phenomena, and one-dimensional pipe element was proposed through Galerkin formation and time integration of the equation. Developed element is combined to pre-developed FEM code for THM phenomena in porous media. Numerical results of Thermal Response Test showed that line-source model overestimates equivalent thermal conductivity of surrounding soils due to thermal interaction between adjacent pipes and finite length of the pipe. Thus, inverse analysis for the TRT simulation was conducted to present optimal transformation matrix with utmost convergence.

• Carbon letters
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• v.13 no.2
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• pp.109-114
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• 2012

A nuclear, biological, chemical (NBC) canister was indigenously developed using active carbon impregnated with ammoniacal salts of copper (II), chromium (VI) and silver (I), and high efficiency particulate aerosol filter media. The NBC canister was evaluated against carbon tetra chloride ($CCl_4$) vapours, which were used as a simulant for persistent chemical warfare agents under dynamic conditions for testing breakthrough times of canisters of gas masks in the National Approval Test of Respirators. The effects of $CCl_4$ concentration, test flow rate, temperature, and relative humidity (RH) on the breakthrough time of the NBC canister against $CCl_4$ vapour were also studied. The impregnated carbon that filled the NBC canister was characterized for surface area and pore volume by $N_2$ adsorption-desorption isotherm at liquid nitrogen temperature. The study clearly indicated that the NBC canister provides adequate protection against $CCl_4$ vapours. The breakthrough time decreased with the increase of the $CCl_4$ concentration and flow rate. The variation in temperature and RH did not significantly affect the breakthrough behaviour of the NBC canister at high vapour concentration of $CCl_4$, whereas the breakthrough time of the NBC canister was reduced by an increase of RH at low $CCl_4$ vapour concentration.

• The Journal of Korean Association of Computer Education
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• v.10 no.1
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• pp.77-88
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• 2007

More flexible learning models are needed, and learning environments that operate through mobile technologies such as portable multimedia players(PMP) provide useful tools in implementing these learning models. The main attractant of PMP is often their versatility: being able to load and play different formats of video, audio, digital images, and interactive media. In this paper, we investigate the factors influencing the usage and acceptance of the PMP for study, based on the extended version of the Technology Acceptance Model (TAM). Based on data collected from online survey, we show that perceived usefulness, perceived ease of use, flow and perceived enjoyment are the major determinants for users to play PMP for study purpose. Factors, including ease of use, contents-credibility are shown to determine the level of perceived usefulness; additionally, perceived usefulness, ease of use and perceived enjoyment are shown to directly affect the level of flow. Based upon the statistical results, some useful guidelines for developing learning contents are also provided.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.11
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• pp.2575-2580
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• 2013

In this paper, we watch out key measure of the level of transportation service about travel time and delay time. And we measured vehicle traffic by moving vehicle method using CCTV which is one of the travel time measure. We should be measured in place of continuous traffic flow characteristics with wide traffic light interval. In addition, traffic flow on the other side of the interval must be sufficiently identifiable and at the end of this section must be possible U-turn. This method it requires only the driver of the vehicle because of the CCTV measure. In addition, We cannot require time, distance, and traffic equipment that can be recorded. Because equipped with the software to do that. In addition to traffic, average travel time, average space speed, traffic density are also available.

• KSBB Journal
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• v.16 no.6
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• pp.632-637
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• 2001

The cells obtained from diesel contaminated site were tested for diesel degradation by culturing them on the culture medium that contained diesel as the only carbon source. Two strains that grew well in the culture media were separated: one formed white colony and another strain formed yellow colony. When they were cultured together, much higher diesel degradation was obtained compares to that of individual cell culture. Mixed culture of white and yellow colony forming strains grew well with 1%(v/v) diesel and the addition of growth nutrients increased the diesel degradation. Additional nitrogen source was efficient for higher diesel degradation (over 90%) when it was compared with that without nitrogen source. When mixed culture of white and yellow colony forming cells were applied to the soil column system contaminated by diesel, 30 mL/min of air flow rate was found to be sufficient to degrade diesel oil. The diesel degradation did not increase noticeably at higher flow rate. The addition of nitrogen source resulted in the increase in diesel degradability.

• Journal of the Korean Wood Science and Technology
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• v.35 no.4
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• pp.61-66
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• 2007

The study was performed to purify and characterize laccase in culture of Trametes versicolor. The fungus was grown in liquid culture media of PDB and added 2,5-xylidine (0.2 mM) after 5 days to enhance the production of laccase. The fungal culture was incubated at $25^{\circ}C$ on a rotary shaker (120 rpm) for 7days, and the culture broth was clarified through Glass filter (GF/C). The aqueous solution was concentrated by ultramicrofiltration (Viva flow 50, GE Healthcare Bioscience, USA) and loaded onto a Hitrap Q FF column. Laccase activity could be detected at one peak, and this enzyme has a molecular mass of approximately 53kDa as determined by SDS-PAGE The optimum pH and temperature for syringaldazine were 5.0 and $60^{\circ}C$, respectively. The specific activity of crude, concentrated and purified laccase were 32, 409, and 1,243 U/mg, respectively.

• Fire Science and Engineering
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• v.11 no.1
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• pp.21-35
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• 1997

The natural convection and combined heat transfer induced by fire in a rectangular enclosure is numerically studied. The model for this numerical analysis is partially opened right wall. The solution procedure includes the standard k-$\varepsilon$ model for turbulent flow and the discrete ordinates method (DOM) is used for the calculation of radiative heat transfer equation. In numerical study, SIMPLE algorithm is applied for fluid flow analysis, and the investigations of combustion gas induced by fire is performed by FAST model of HAZARD I program. In this study, numerical simulation on the combined naturnal convection and radiation is carried out in a partial enclosure filled with absorbed-emitted gray media, but is not considered scattering problem. The streamlines, isothermal lines, average radiation intensity and kinetic energy are compared the results of pure convection with those of the combined convection-radiation, the combined heat transfer. Comparing the results of pure convection with those of the combined convection-radiation, the combined heat transfer analysis shows the stronger circulation than those of the pure convection. Three different locations of heat source are considered to observe the effect of heat source location on the heat transfer phenomena. As the results, the circulation and the heat transfer in the left region from heating block are much more influenced than those in the right region. It is also founded that the radiation effect cannot be neglected in analyzing the building in fire. And as the results of combustion gas analysis from FAST model, it is found that O2 concentration is decreased according to time. While CO and CO2 concentration are rapidly increased in the beginning(about 100sec), but slowly decreased from that time on.