• International Journal of Oral Biology
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• 제34권1호
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• pp.1-6
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• 2009

The purpose of the present study was to examine the role of peripheral nitric oxide (NO) pathways in the onset of interleukin (IL)-1$\beta$-induced mechanical allodynia in the orofacial area. Experiments were carried out on male Sprague-Dawley rats weighing 230-280 gm and surgical procedures were performed under pentobarbital sodium (40 mg/kg, i.p.). Under anesthesia, a polyethylene tube (PE10) was implanted into the subcutaneous area of one vibrissa pad, which enabled the injection of IL-1$\beta$ or other chemicals. We subcutaneously injected 50 ${\mu}L$ of IL-1$\beta$ into a vibrissa pad through the implanted polyethylene tube with a 100 ${\mu}L$ Hamilton syringe. After the administration of 0.01, 0.1, 1, or 10 pg of IL-1$\beta$, withdrawal behavioral responses were examined. The subcutaneous injection of saline had no effects on the air-puff thresholds. Following the subcutaneous injection of 0.01, 0.1, 1, or 10 pg of IL-1$\beta$, the threshold of air puffs decreased significantly to 12 $\pm$ 3, 7 $\pm$ 2, 5 $\pm$ 1, or 5 $\pm$ 1 psi, respectively, in a dose dependent manner. Pretreatment with L-NAME, a nitric oxide synthase (NOS) inhibitor, blocked IL-1$\beta$-induced mechanical allodynia. However, neither D-NAME, an inactive isomer of L-NAME, nor vehicle affected the IL-1$\beta$-induced mechanical allodynia. Subcutaneous injection of IL-1$\beta$ increased the number of c-fos-like immunoreactive neurons, whereas pretreatment with L-NAME decreased this number, in the trigeminal caudal nucleus. These results suggest that pro-inflammatory cytokines and NO are important contributors to the pathogenesis of persistent and exaggerated IL-1$\beta$-induced pain states. Based on these observations, peripheral application of NOS inhibitors may be of therapeutic value in treating pain disorders in the clinic.

• 대한기계학회논문집B
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• 제22권5호
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• pp.563-572
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• 1998

An experimental study of hot filament chemical vapor deposition(HFCVD) has been carried out for the fabrication of diamond thin film. Of particular interest is the measurement of deposition uniformity on large substrates. Experimental apparatus including a vacuum chamber, heating elements, etc. has been designed and manufactured. Deposition profiles for different pretreatment powders and different flow rates have been measured in conjunction with the measurement of substrate temperature distribution on a large substrate surface. As the flow rate increases, deposition rate increases, however, the crystallinity becomes worse. Higher growth rate has been found on the region closer to the center location where substrate temperature is higher. The crystallinity has been improved as gas flow rate decreases. The growth rate and morphology of deposition were identified by SEM and the existence of diamond phase was proved by Raman spectroscopy.

• 패션비즈니스
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• 제7권6호
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• pp.50-56
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• 2003

Chitosan has been widely applied to the products in various industries such as textile fabrics, apparels, foods, medical area, etc. Cochineal has long been employed as one of natural dyestuffs in the textile industry. The effect of chitosan pre-treatment on the low-stress physical and mechanical properties of cochineal-dyed fabrics including cotton, silk, nylon and polyester fabrics was investigated in this study. The chitosan treatment and mordanting of the fabrics changed the bending, shear, compression, and surface properties of the fabrics. In cotton fabric specimens, while the increase of B(bending rigidity) of cotton is relatively high, the increase of G(shear rigidity) of cotton is relatively low. In nylon and PET fabric specimens, while the increase tendency of B is relatively low, that of G is high compared to the corresponding cotton fabrics.

• 유기물자원화
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• 제17권3호
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• pp.82-90
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• 2009

본 연구에서는 기계식 전처리에 의한 하수 슬러지의 가용화 효과를 회분식 실험을 통해 조사하였다. 기계식 전처리를 통한 하수슬러지의 가용화는 세포벽의 파괴로 통해 용존성 화학적 산소요구량, 단백질 및 탄수화물의 농도를 증가시키는 것으로 나타났다. 알칼리와 기계식 전처리를 병행하여 슬러지를 가용화한 결과 기계식 전처리만을 수행한 경우에 비해 용존성 화학적 산소요구량이 높은 것으로 나타났다. 혐기성 생분해도 측면에서 기계식 전처리는 메탄 발생량을 증가시키는 것으로 나타났다. 기계식과 알칼리 및 기계식 전처리를 동시에 수행한 경우 각각 24.1%와 44.5%의 생화학적 메탄 잠재능을 향상시키는 것으로 나타나 하수슬러지의 가용화는 혐기성 생분해도 향상에 효과적이다.

• Journal of Chest Surgery
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• 제43권6호
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• pp.565-575
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• 2010

배경: 이식편을 개발함에 있어서 숙주의 면역반응을 최소화하여 보다 더 오래 사용할 수 있게 하기위한 방법으로 무세포화에 대한 연구가 시행되고 있다. 저자들은 소 심낭의 무세포화의 과정에 효소제인 트립신 전처치가 물리역학적, 조직학적으로 미치는 영향에 대해 알아보고자 하였다. 대상 및 방법: 소 심낭편을 SDS와 Triton X-100 또는 N-lauroylsarcosinate와 Triton X-100으로 무세포화하는 것을 기본으로 한 군들과 0.1% 트립신/0.1% EDTA로 전처치를 추가한 군들에서 장력 검사를 실시하고, 생체 이식 후의 상태를 가정한 피로도 검사를 전후로 하여 투과도와 유순도는 검사하였고, 피로도 검사 전후로 조직학적인 변화를 광학현미경 및 전자현미경으로 관찰하였다. 결과: 트립신 처치를 추가한군과 아닌 군에서 기계적 장벽의 차이는 없었으나, 투과도와 유순도는 피로도검사 전과 후로 트립신 처치를 하지 않은 군에 비해 증가하였으며, 조직학적으로도 세포외 기질이 더 손상된 소견을 보였다. 걸론: 소 심낭의 무세포화에서 트립신 전처치는 세포외 기질의 손상을 유발하지만 기계적 장력에는 큰 영향을 미치지 않았으며, 피로도검사 전후 모두 투과도와 유순도를 증가시켰다. 무세포화과정에서 트립신과 같은 단백질 분해 효소제를 이용하기 위해서는 조직의 생체 물리적 손상을 최소화할 수 있는 다양한 방법을 조합한 연구가 더 필요하다.

• The Korean Journal of Pain
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• 제35권4호
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• pp.433-439
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• 2022

Background: Repeated administration of opioid analgesics for pain treatment can produce paradoxical hyperalgesia via peripheral and/or central mechanisms. Thus, this study investigated whether spinally (centrally) administered orexin A attenuates opioid-induced hyperalgesia (OIH). Methods: [D-Ala², N-Me-Phe⁴, Gly⁵-ol]-enkephalin (DAMGO), a selective µ-opioid receptor agonist, was used to induce mechanical hypersensitivity and was administered intradermally (4 times, 1-hour intervals) on the rat hind paw dorsum. To determine whether post- or pretreatments with spinal orexin A, dynorphin A, and anti-dynorphin A were effective in OIH, the drugs were injected through an intrathecal catheter whose tip was positioned dorsally at the L3 segment of the spinal cord (5 ㎍ for all). Mechanical hypersensitivity was assessed using von Frey monofilaments. Results: Repeated intradermal injections of DAMGO resulted in mechanical hypersensitivity in rats, lasting more than 8 days. Although the first intrathecal treatment of orexin A on the 6th day after DAMGO exposure did not show any significant effect on the mechanical threshold, the second (on the 8th day) significantly attenuated the DAMGO-induced mechanical hypersensitivity, which disappeared when the type 1 orexin receptor (OX1R) was blocked. However, intrathecal administration of dynorphin or an anti-dynorphin antibody (dynorphin antagonists) had no effect on DAMGO-induced hypersensitivity. Lastly, pretreatment with orexin A, dynorphin, or anti-dynorphin did not prevent DAMGO-induced mechanical hypersensitivity. Conclusions: Spinal orexin A attenuates mechanical hyperalgesia induced by repetitive intradermal injections of DAMGO through OX1R. These data suggest that OIH can be potentially treated by activating the orexin A-OX1R pathway in the spinal dorsal horn.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 2002년도 proceedings of the second asia international conference on tribology
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• pp.371-372
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• 2002

It is well known that red blood cells (RBCs) are suffered from chronic stresses in systemic circulation. The objective of this study is to clarify the effect of the aging of RBCs on rheological properties and hemolysis. Initially, RBCs age fractionation was performed by using a high-speed centrifugation (15[min] at 1500[G]), then young and aged RBCs were suspended in plasma to adjust the hematocrit level of 40[%]. After this pretreatment, the viscosity was measured by using a capillary type and a cone-plate type viscometers, respectively, and the hemolysis test was carried out by a seesaw type shaker. Results from these experiments showed that the viscosity of the aged RBCs measured by the capillary viscometer was increased by 10[%] as compared with that of the young RBCs. Under the condition of all shear zones, the viscosity of the aged RBCs was increased in case of using the cone-plate type viscometer. And the hemolytic level was increased twice as the aging. The data obtained in this study indicated that the ability of aggregation of RBCs was increased and the deformability of RBCs membrane got lower with the aging. Furthermore, it was exhibited that the fragility of RBCs ’ membrane was increased with the aging.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 1999년도 Proceedings of Pre-symposium of the 10th ISWPC
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• pp.172-177
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• 1999

Effect of fiber treatment with cellulase (Liftase A40), and with two refining methods (Valley beating and impact refining) on wood fiber and handsheet properties were investigated at three refining levels (300, 400, and 500ml) for two furnishes (NBKP and LBKP). Part of the treated furnishes were classified by 150 mesh screen into fine-free fiber, and fines. Fiber length analysis, WRV, zero-span strength, and other handsheet mechanical properties were compared. The study showed that Liftase A40 lowered the zero-span and the folding endurance of both furnishes (NBKP much more and LBKP much less). Pretreatment with Liftase A 40 followed by refining significantly lowered the fiber length and refining energy to reach to the target freeness. Impact refining, which is done by hitting the fibers vertically with rod at 20% solid content, kept the fiber length increased WRV, and improved handsheet mechanical properties much more than valley beating. Properties of fines from different sources were compared in detail in the study.

• 한국수소및신에너지학회논문집
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• 제30권1호
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• pp.49-57
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• 2019

Biomass, a carbon-neutral fuel, has great advantages because it can replace fossil fuels to reduce greenhouse gas emissions. However, due to its low density, high water content, and hydrophilicity, biomass has disadvantages for transportation and storage. To improve these properties, a pretreatment process of biomass is required. One of the various pre-treatment technologies, torrefacion, makes biomass similar to coal through low-temperature pyrolysis. In this study, torrefacion treatment was carried out at 200, 230, 250, 280, and $300^{\circ}C$ for wood pellet, empty fruit bunch (EFB) and kenaf, and the feasibility of replacing coal with fuel was examined. Hygroscopicity tests were conducted to analyze the hydrophobicity of biomass, and its chemical structure changes were investigated using Infrared spectrum analysis. It was confirmed that the hygroscopicity was decreased gradually as the torrefacion temperature increased according to the hygroscopicity tests. The hydrophilicity was reduced according to the pyrolysis of hemicellulose, cellulose, and lignin of biomass.

• The Korean Journal of Pain
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• 제9권2호
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• pp.311-317
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• 1996

Background: Following peripheral nerve injury, rats will show a tactile allodynia and hyperalgesia. But the mechanism of allodynia is still obscure. Previous studies have shown this allodynia was reversed by intrathecal alpha-2 agonists and NMDA antagonists, but not by morphine. In formalin test, either the pretreatment of NMDA antagonist or morphine prevents the hyperalgesia. The present studies, using rats rendered allodynic by ligation of the left L5 and L6 nerves, aimed to investigate the effects of pretreatment of MK-801 and morphine on the development of tactile allodynia. Methods and Material: Male Sprague-Dawley rats (100~150g) were anesthetized with halothane, the left L5 and L6 spinal nerves were ligated tightly by 6-0 black silk. For sham operation muscle dissection was performed but the spinal nerve was not ligated. For pretreatment of drugs, MK-801 (NMDA antagonist; 0.3 mg/kg). CNQX (non-NMDA) antagonist; 0.3 mg/kg), morphine (1 mg/kg) or saline (placebo) was administered subcutaneously 30 minutes before operation. A second dose was administered subcutaneously 24 hours after operation and further doses were given daily for 2 days further. The volume of injection was 5 ml/kg. To assess the mechanical allodynia, paw withdrawal thresholds of ipsilateral limb were determined using 8 von Frey hairs. Results: Within 2 days saline, CNQX or morphine injected rats developed tactile allodynia (paw withdrawal threshold was about 2g), and persisted for over 2 weeks. Pretreatment of MK-801 delayed the development of tactile allodynia for 3 days comparing to that of saline injected rat. Conclusion: NMDA receptor in the central nerve system plays an important role in the development of tactile allodynia induced by peripheral nerve injury. But the mechanism may be different from hyperalgesia developed in formalin test.