• 폴리머
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• 제27권4호
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• pp.307-312
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• 2003

고밀도 폴리에틸렌 (HDPE)과 초고분자량 폴리에틸렌 (UHMWPE)을 혼합하여 중공사막을 제조하고, 이의 형태구조와 물성 변화를 조사하였다. 분자량 측정과 FT-IR을 이용한 분석 결과, 상품화된 중공사막(Sterapore)은 고분자량의 HDPE로 제조되었으며, 표면은 친수성 고분자인 비닐 알코올/비닐 아세테이트 공중합체로 코팅되어 높은 수투과도를 갖는 이유를 규명하였다. HDPE/UHMWPE 블렌드에서 UHMWPE의 혼합 한계 조성비는 10 wt% 이하이며, UHMWPE의 분산성을 높이기 위하여 혼합과정에서 광유를 도입하여야 할 것으로 판단되었다. 제조된 HDPE/UHMWPE 블렌드 중공사의 기계적 물성과 막 구조는 Sterapore와 유사하였다.

• 한방재활의학과학회지
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• 제30권4호
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• pp.31-39
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• 2020

Objectives Danggwisoo-san and Jakyakgamcho-tang are frequently prescribed for traffic accident patients in Korea. The aim of this study was to examine index compound analysis, antioxidant activity and amount of starch measurement by extraction method. Methods Danggwisoo-san and Jakyakgamcho-tang were extracted with water and 70% ethanol. Antioxidant activity was measured by 2,2-Diphenyl-1-picrylhydrazyl, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and ferric reducing antioxidant power according to the standard protocol. The contents of the indicator components nodakenin, paeoniflorin, and glycyrrhizin were measured by high-performance liquid chromatography, respectively. All starches were hydrolyzed and then total D-glucose was measured and compared. Results Antioxidant activity was excellent in 70% ethanol in all assays. The index component was jagged because its solubility was different depending on the extraction solvent. Starch content was significantly lower in 70% alcohol extract than water extract. Conclusions The results of this study showed that physiological activities and components are different according to extraction conditions. Each herbal medicine has a suitable extraction solvent. Also, the difference in starch content is an object to be considered as it may affect digestion and absorption.

• Applied Biological Chemistry
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• 제29권2호
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• pp.227-236
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• 1986

찹쌀 및 가루를 사용하여 담금한 고추장(시험구 A:찹쌀가루, 시험구 B: 찹쌀, 시험구 C: 찹쌀 및 가루의 혼용)의 화학성분, 효소력 및 texture를 측정한 결과는 다음과 같다. 수분, 조단백, 조지방, 적정산도, 무기성분, alcohol의 함량 및 pH는 시험구간에 차이가 없었다. 아미노태 질소함량과산성 protease 및 당화 amylase 활성은 A구가 높았고 환원당은 B구가 경시적으로 높았다. Ca, Cu, K, Mn, Mg, P, Fe의 무기성분이 각 시험구에서 검출되었고 이중 K의 함량이 가장 높았으며 Cu, Mn, Fe은 극히 미량이었다. Aspartic acid, glutamic acid, cystine, agrginine, proline등의 유리아미노산이 60일 숙성 고추장에서 양적으로 많이 검출되었고 histidine, valine의 함량은 적었으며, 유리아미노산의 총량은 A구 16.49, B구 13.68, C구 15.84mg/g으로서 A구가 가장 많았고 B구의 고추장이 가장 적었다. Texture를 측정한 결과 견고성과 부착성은 담금 직후에는 A구가, 숙성 후기에는 B구가 높았으며 응집성, 탄력성은 A구가 높았고 B구가 낮았다.

• Journal of Ginseng Research
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• 제39권2호
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• pp.105-115
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• 2015

Background: Alcoholic steatosis is the earliest and most common liver disease, and may precede the onset of more severe forms of liver injury. Methods: The effect of Korean Red Ginseng extract (RGE) was tested in two murine models of ethanol (EtOH)-feeding and EtOH-treated hepatocytes. Results: Blood biochemistry analysis demonstrated that RGE treatment improved liver function. Histopathology and measurement of hepatic triglyceride content verified the ability of RGE to inhibit fat accumulation. Consistent with this, RGE administration downregulated hepatic lipogenic gene induction and restored hepatic lipolytic gene repression by EtOH. The role of oxidative stress in the pathogenesis of alcoholic liver diseases is well established. Treatment with RGE attenuated EtOH-induced cytochrome P450 2E1, 4-hydroxynonenal, and nitrotyrosine levels. Alcohol consumption also decreased phosphorylation of adenosine monophosphate-activated protein kinase, which was restored by RGE. Moreover, RGE markedly inhibited fat accumulation in EtOH-treated hepatocytes, which correlated with a decrease in sterol regulatory element-binding protein-1 and a commensurate increase in sirtuin 1 and peroxisome proliferator-activated receptor-a expression. Interestingly, the ginsenosides Rb2 and Rd, but not Rb1, significantly inhibited fat accumulation in hepatocytes. Conclusion: These results demonstrate that RGE and its ginsenoside components inhibit alcoholic steatosis and liver injury by adenosine monophosphate-activated protein kinase/sirtuin 1 activation both in vivo and in vitro, suggesting that RGE may have a potential to treat alcoholic liver disease.

• 센서학회지
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• 제6권3호
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• pp.214-220
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• 1997

본 연구에서는 생화학 물질을 정량적으로 분석하기 위하여 소산장(Evanescent field) 흡수를 이용한 박막 광도파로형 칼륨이온센서를 제작하였다. 도파로는 Pyrex glass($26{\times}19{\times}1mm$, $n_{1}=1.485$ at 514nm, Ar laer ; Coherent 사 M532) 기판위에 RF sputtering법으로 Coming-7059 glass($n_{2}=1.588$, at 514nm, Ar laer ; Coherent사 M532) 2 종류의 박막 광도파로를 형성하였으며, 그 두께는 프리즘 결합법으로 측정한 결과 각각 $T_{1}=1.04{\mu}m$ 와 $T_{2}=1.41{\mu}m$ 였다. 칼륨이온 선택성의 이온감지막은 변색성 이온감응물질인 ETH 5294와 중성이온감응물질인 valinomycin을 poly(vinyl chloride-co-vinyl acetate-co-vinyl alcohol) ( 91 : 3 : 6 ) 공중합체 막내에 포괄법으로 고정화한 후 도파로 위에 스핀코팅법으로 제조하였다. 그리고, 센서의 특성을 평가하기 위해 감지막의 작용길이, 도파로의 두께 및 변색성 이온감응물질의 조성비 변화에 따른 감도 의존성을 조사하였다. 본 센서는 약 $1{\times}10^{-6}M\;{\sim}1.0\;M$의 넓은 측정범위를 가지며, 90%의 응답시간은 약 1분 이내의 빠른 응답특성을 나타내었다. 또한 분광분석법에 의한 투과광도법 및 광섬유를 이용한 optode형 센서를 제작하여 본 센서와 그 특성을 비교한 결과, 본 센서의 우수성을 알 수 있었다. 따라서 본 센서는 생화학 및 의용, 환경감시 분야 등에 응용 가능할 것으로 사료된다.

• Journal of Nutrition and Health
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• 제10권2호
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• pp.27-34
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• 1977

The present study was carried out to evaluate the nutritional quality of rabbit meat protein. The composition of amino acids contained in rabbit meat was compared with those of other animal meats such as beef, pork and chicken. Also included in this study was the question whether the cooking and storage conditions affect the amino acid composition and the pepsindigestibility of rabbit meat protein. The results are summarized as follows: 1. The large variation observed from sample to sample of EAA (essential amino acid) composition in rabbit meat was found to be an interesting but peculiar property of rabbit meat protein. The most limiting amino acid of rabbit meat protein was phenylalanine, whereas methionine was the first limiting amino acid of both beef and pork proteins. Chemical scores of various meat proteins were 68, 65, 66, and 74 for rabbit meat, beef, pork, and chicken respectively. 2. In pan roasting, the EAA damaged most by heat was methionine (15%). When cooked after two months of frozen storage, lysine decreased most. 3. Higher pepsin digestibility was obtained by cooking rabbit meat after seasoned in alcohol, ginger juice, and other spices compared with various other cooking conditions without seasoning. The pepsin digestibility value was even higher for the seasoned meat than for the raw meat. 4. Among various meats tested the rabbit meat showed the lowest pepsin digestibility. 5. A simple measurement of released methionine could be used to determine relative digestibility instead of measuring $NH_2-N$ content after pepsin digestion. From all the results obtained in this study it can be concluded that rabbit meat is a good Protein food item when used fresh and stored properly to prevent rancidity problems. It is suggested to study further the peroxidation effect of unsaturated fatty acids on protein quality. This study was supported by the Ministry of Science and Technology in Korea.

• 대한한방내과학회지
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• 제40권6호
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• pp.1112-1121
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• 2019

Objectives: FDY003 is a raw material for medicine consisting of a natural product that is expected to have the advantages of low side effects and high efficacy. In this study, we predict the efficacy and the standardization of the drug by method validation of anticipated index compounds and the measurement of antioxidant activity. Methods: FDY003 is prepared by extracting and purifying 70% of ethyl alcohol (EtOH). The method validation of cordycepin and chlorogenic acid was determined by high-performance liquid chromatography-photo diode array (HPLC-PDA) and the content of FDY003 was calculated. In order to monitor the biological activity of FDY003, antioxidant activity was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric-reducing antioxidant power (FRAP). The equivalent values of antioxidants such as trolox, ascorbic acid, gallic acid, and caffeic acid were measured by ABTS and FRAP. Results: Chlorogenic acid and cordycepin were both found suitable for method validation in HPLC and FDY003 containing 9.92±0.50 and 17.97±0.27 ㎍/g, respectively. In DPPH, the electron donating ability (EDA) value of FDY003 was increased in a concentration dependent manner. FDY003 confirmed antioxidant activity by ABTS and FRAP. Conclusions: FDY003 contains certain components including cordycepin and chlorogenic acid and has antioxidant ability by various mechanisms. Therefore, it is expected that FDY003 is capable of various physiological activities including anti-cancer activity.

• 한국수소및신에너지학회논문집
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• 제24권1호
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• pp.44-49
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• 2013

Hydrogen penetration into a metal leads to damages and mechanical degradations and its content measurement is of importance. For a precise measurement, a sample preparation procedure must be optimized through a series of studies on sample washing and drying. In this study, two-step washing with organic solvents and thermal soaking in inert gas were tried with a rod-shaped, API X65 steel sample. The samples were machined from a steel plate and then washed in acetone and etyl-alcohol for 5 minute each and dried with compressed air. After then, the samples were thermally soaked in a home-made nitrogen gas chamber during 10 minute at different heat gun temperatures from 100 to $400^{\circ}C$ and corresponding temperature range in the soaking chamber was from 77 to $266^{\circ}C$ according to the temperature calibration. Hydrogen residue in the samples was measured with a hot extraction system after each soaking step; hydrogen residue of $0.70{\pm}0.12$ wppm after the thermal soaking at $77^{\circ}C$ decayed with increase of the soaking temperature. By adopting the heat transfer model, decay behavior of the hydrogen residue was fitted into an exponential decay function of the soaking temperature. Saturated value or lower bound of the hydrogen residue was 0.36 wppm and chamber temperature required to lower the hydrogen residue about 95% of the lower bound was $360^{\circ}C$. Furthermore, a thermal desorption spectroscopy was done for the fully soaked samples at $360^{\circ}C$. Weak hydrogen peak was observed for whole temperature range and it means that hydrogen-related contaminants of the sample surface are steadily removed by heating. In addition, a broad peak found around $400^{\circ}C$ means that parts of the hydrogen residue are irreversibly trapped in the steel microstructure.

• 한국안전학회지
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• 제30권1호
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• pp.72-78
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• 2015

According to the report of the WHO, workers have been exposed to much job strain such as job load, responsibility, role, interpersonal conflict etc. In Korea, studies on job strain started to become active from 1990s and now hundreds of studies are actively under going or publishing so that the studies are contributing to development and improvement of job strain. Representative measurement models of job strain are Job Strain Model of Karasek, Job Stress Model of NOISH, Korea Occupational Stress Scale, JSQ(Job Stress Questionnaire), K-OSI(Korea Version of Occupational Stress Inventory) etc. (Lee Kwan-Suk, 2012 ; KOSHA, 2003). Among them, Job Strain Model of Karasek had been loved by many researchers of job strain before Korea Occupational Stress Scale was developed. Job Strain Model of Karasek had been fitted to Korean style and then, used to analyze job strain of Korean people so that this Scale highly contributed to seeking relationship with cardiovascular disease, musculoskeletal disease caused by job, smoking, drug, alcohol poisoning, and pulse(Lee Kwan-Suk, 2012). But as this Model was studied and developed based on foreign culture and life pattern, a model fit to Korea was developed to measure job strain for Korean people, which is Korea Occupational Stress Scale now most frequently used in measuring job strain. Accordingly, after this study made questionnaire survey about same population using the two methods used most frequently in measuring job strain, the study investigated what features appeared, what correlations appear between two models, and comparatively analyzed characteristics each independent and dependent variable. Based on this, the study aimed to exactly express job strain of Korean people. The subjects of the study were a population of 233, and Karasek's Questionnaire and KOSS's Questionnaire were surveyed at the same time. The results were analyzed by statistical program to obtain significant difference between two models. Four particular groups were divided with Job Strain Model of Karasek and the four particular groups were measured with Korea Occupational Stress Scale. And job strain come from combination of two models was measured, with which new comparative analysis method was suggested.

• 대한본초학회지
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• 제33권1호
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• pp.37-46
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• 2018

Objectives : Liver disease is an inflammatory reaction caused by oxidative stress, viral, alcohol, and drug properties. Inflammatory reaction causes hepatitis and chronic hepatitis is persistent, it progresses to liver fibrogenesis and liver cancer. The aim of this study was to confirm the hepatoprotective effect of Tongyuhwalhyeol-tang(Tongqiaohuoxue Decoction) (TH) and Gamtongyuhawlhyeol-tang(GTH) in TAA-induced liver injury animal model. Methods : The antioxidant activities were evaluated through in vitro experiments, such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2'-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays, total polyphenol and total flavonoid content measurement. To confirm the liver protective effect, induced by Thioacetamide (TAA) for 3 days injection at 200 mg/kg rats. TH and GTH were treated 3 days at 200 mg/kg/day. The changes of reactive oxygen species (ROS), peroxynitrite ($ONOO^-$), alanine aminotransferanse (ALT) and aspartate aminotransferase (AST) in serum were analyzed after experiment. Also, expression of anti-inflammation, anti-oxidant related proteins were investigated by western blot analysis. Results : TH was inhibited the antioxidant activities. In the TAA-induced rat, TH decreased ROS, $ONOO^-$, ALT, AST level in serum. Inflammation related protein expressions increased in TAA-induced rat compared to normal rat. However, TH group inhibited the down expression of these proteins. Also, anti-oxidant related protein expressions increased in TH group compared TAA-induced rat. Conclusion : Therefor, these results suggested that TH provided hepatoprotective effects on the hepatic injury leading to the reduction of inflammatory response. In addition, the effect of TH was superior to that of GTH.