The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Protective Effect of Tongyuhwalhyeol-tang on Liver Injury in Thioacetamide-induced Rat

Thioacetamide 유발 간손상모델에서 통규활혈탕의 간보호효과

  • Kim, Kyeong Jo (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Soo Hyun (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Su Ji (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Ah Reum (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kwon, Ojun (Gyeongbuk Institute for Regional Program Evaluation) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 김경조 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수현 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수지 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이아름 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 권오준 (경북지역사업평가단) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2017.12.11
  • Accepted : 2018.01.15
  • Published : 2018.01.30
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Abstract

Objectives : Liver disease is an inflammatory reaction caused by oxidative stress, viral, alcohol, and drug properties. Inflammatory reaction causes hepatitis and chronic hepatitis is persistent, it progresses to liver fibrogenesis and liver cancer. The aim of this study was to confirm the hepatoprotective effect of Tongyuhwalhyeol-tang(Tongqiaohuoxue Decoction) (TH) and Gamtongyuhawlhyeol-tang(GTH) in TAA-induced liver injury animal model. Methods : The antioxidant activities were evaluated through in vitro experiments, such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2'-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays, total polyphenol and total flavonoid content measurement. To confirm the liver protective effect, induced by Thioacetamide (TAA) for 3 days injection at 200 mg/kg rats. TH and GTH were treated 3 days at 200 mg/kg/day. The changes of reactive oxygen species (ROS), peroxynitrite ($ONOO^-$), alanine aminotransferanse (ALT) and aspartate aminotransferase (AST) in serum were analyzed after experiment. Also, expression of anti-inflammation, anti-oxidant related proteins were investigated by western blot analysis. Results : TH was inhibited the antioxidant activities. In the TAA-induced rat, TH decreased ROS, $ONOO^-$, ALT, AST level in serum. Inflammation related protein expressions increased in TAA-induced rat compared to normal rat. However, TH group inhibited the down expression of these proteins. Also, anti-oxidant related protein expressions increased in TH group compared TAA-induced rat. Conclusion : Therefor, these results suggested that TH provided hepatoprotective effects on the hepatic injury leading to the reduction of inflammatory response. In addition, the effect of TH was superior to that of GTH.

Keywords

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