• Title/Summary/Keyword: Measured stage

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Developmental Regulation of Caenorhabditis elegans DNA Topoisomerase I Expression

  • Jang, Yeon-Joo;Park, Hyung-Ki;Lee, Jun-Ho;Koo, Hyeon-Sook
    • BMB Reports
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    • v.31 no.3
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    • pp.249-253
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    • 1998
  • The developmental regulation of Caenorhabditis elegans DNA topoisomerase I expression was examined using synchronized Caenorhabditis elegans cultures. Variations of the relative mRNA and protein levels of the enzyme during their development were measured by Northern and Western analyses, respectively. The mRNA level was the highest at the embryonic stage, decreasing rapidly to the one tenth level at the L1 stage, and then increasing by a few fold at the L4 and young adult stages. The protein level was the highest at the L1 stage, with gradual decreasing at the following stages until it showed a slight increase at the young adult stage. Based on our results of the expressional regulation, the possible roles of DNA topoisomerase I in the development of C. elegans are discussed.

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Automation of laser scanning and registration of measured data using a 3-axis motorized stage (3축 전동테이블을 이용한 레이저 스캐너의 측정 및 레지스트레이션 자동화)

  • Son, Seok-Bae;Kim, Seung-Man;Lee, Kwan-Heng
    • Proceedings of the KSME Conference
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    • 2001.06c
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    • pp.134-139
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    • 2001
  • Laser scanners are widely used for reverse engineering and inspection of freeform parts in industry such as motors, electronic products, dies and molds. Due to the lack of measuring software and positioning device, the laser scanning processes have been erroneous and inconsistent. In order to automate measuring processes, an automated scan plan generation software and a proprietary hardware are developed. In this paper, an automated laser scanning system using a 3-axis motorized stage is proposed. In the scan planning step, scan directions, paths, and the number of scans are generated considering optical and mechanical parameters. In the scanning step, the generated scan plan is downloaded into the laser scanner and the motorized stage and the points on the surface are captured automatically. Finally, the point data set is analyzed to evaluate the performance of the system.

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A multivariate latent class profile analysis for longitudinal data with a latent group variable

  • Lee, Jung Wun;Chung, Hwan
    • Communications for Statistical Applications and Methods
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    • v.27 no.1
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    • pp.15-35
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    • 2020
  • In research on behavioral studies, significant attention has been paid to the stage-sequential process for multiple latent class variables. We now explore the stage-sequential process of multiple latent class variables using the multivariate latent class profile analysis (MLCPA). A latent profile variable, representing the stage-sequential process in MLCPA, is formed by a set of repeatedly measured categorical response variables. This paper proposes the extended MLCPA in order to explain an association between the latent profile variable and the latent group variable as a form of a two-dimensional contingency table. We applied the extended MLCPA to the National Longitudinal Survey on Youth 1997 (NLSY97) data to investigate the association between of developmental progression of depression and substance use behaviors among adolescents who experienced Authoritarian parental styles in their youth.

Selection of Lecanicillium Strains with High Virulence against Developmental Stages of Bemisia tabaci

  • Park, Hee-Yong;Kim, Keun
    • Mycobiology
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    • v.38 no.3
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    • pp.210-214
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    • 2010
  • Selection of fungal strains with high virulence against the developmental stages of Bemisia tabaci was performed using internal transcribed spacer regions. The growth rate of hyphae was measured and bioassay of each developmental stage of B. tabaci was conducted for seven days. All of the fungal strains tested were identified as Lecanicillium spp., with strain 4078 showing the fastest mycelium growth rate (colony diameter, 16.3 $\pm$ 0.9 mm) among the strains. Compared to strain 4075, which showed the slowest growth rate, the growth rate of strain 4078 was increased almost 2-fold after seven days. Strains 4078 and Btab01 were most virulent against the egg and larva stages, respectively. The virulence of fungal strains against the adult stage was high, except for strains 41185 and 3387. Based on the growth rate of mycelium and level of virulence, strains 4078 and Btab01 were selected as the best fungal strains for application to B. tabaci, regardless of developmental stage.

Development of Multi-axis Nano Positioning Stage for Optical Alignment (광소자 정렬용 극초정밀 다축 위치 제어장치 개발)

  • 정상화;이경형;차경래;김현욱;최석봉;김광호;박준호
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.10a
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    • pp.304-307
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    • 2004
  • As optical fiber communication grows, the fiber alignment become the focus of industrial attention. This greatly influence the overall production rates for the opto-electric products. We proposed multi-axis nano positioning stage for optical fiber alignment. This device has 3 DOF translation and sub nanometer resolution. This nano stage consist of 3 PZT-driven flexure stages which are stacked parallel. The displacement of it is measured with capacitance gauge and is controlled by computer-embedded main controller. The design process of flexure stage using FEM is proposed and the performance evaluation of this system is verified with experiments.

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Numerical Simulation of Advection and Diffusion using the Local Wind Model in Pusan Coastal Area, Korea (부산 연안역에서의 국지풍모델을 이용한 이류확산 수치모의)

  • 김유근;이화운;전병일
    • Journal of Korean Society for Atmospheric Environment
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    • v.12 no.1
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    • pp.29-41
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    • 1996
  • The two-stage numerical model was used to study the relation between three-dimensional local wind model, advection/diffusion model of random walk method and second moment method on Pusan coastal area. The first stage is three dimensional time-dependent local wind model which gives the wind field and vertical dirrusion coefficient. The second stage is advection/diffusion model which uses the results of the first stage as input data. First, wind fields on Pusan coastal area for none synoptic scale wind showed typical land and sea breeze circulation, and convergence zone occured at 1200LST in northern of domain, in succession, moved northward of domain. Emissions from Sinpyeong industrial district were trasnported toward the inland by sea breeze during daytime, and reached the end part of domain about 1800LST. During nighttime, emissions return to sea by land breeze and vertical diffusion also contributes to upward transport. In order to use this model for forecast of air pollution concentration on the Pusan coastal area, it is necessary that computed value must be compared with measured value and wind fields model must also be dealt in detail.

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Design and Characteristics of X-band Monolitic Series Feedback LNA using 0.5$\mu\textrm{m}$GaAs MESFET (0.5$\mu\textrm{m}$-GaAs MESFET을 이용한 X-밴드 모노리식 직렬 궤환 LNA의 설계 및 특성)

  • 전영진;김진명;정윤하
    • Journal of the Korean Institute of Telematics and Electronics D
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    • v.34D no.5
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    • pp.7-13
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    • 1997
  • A X-band 3-stage monolithic LNA (low noise amplifier) with series feedback has been successfully desined and demonstrated by suign 0.5-$\mu\textrm{m}$ GaAs MESFET. In the design of the 3-stage LNA, the effects of series feedback to the noise figure, the gain, and the stability have been investigated ot find the optimal short stub length. As a result, the inductive series feedback topology which has 10degree short stub in the GaAs MESFET source lead, has been employed in the 1-st stage. The fabricated MMIC LNA's chip size is only 1mm$^{2}$/stage, which is smaller than the previously reported X-band MMIC input/output return losses are less than -10dB and -15dB, respectively. The noise figure (NF) is less than 2.6dB. The measured data show good agreement with the simulated values.

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An Experimental Study on Pumping Speed of Disk-Type Drag Pumps for Spiral Channels in Rarefied Gas Flows (희박기체영역에서의 나선형 홈을 가진 원판형 드래그펌프의 배기속도에 관한 실험적 연구)

  • Kwon, Myoung-Keun;Yang, Seoung-Min;Lee, Seung-Jae;Hwang, Young-Kyu;Heo, Joong-Sik
    • Proceedings of the KSME Conference
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    • 2003.04a
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    • pp.2101-2104
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    • 2003
  • Experimental investigations are performed for the rarefied gas flows in a disk-type drag pump (DTDP). The pump considered in this paper consists of grooved spiral channel on rotors and planar stators. The flow-metre method is adopted to calculate the pumping speed. Compression ratio and pumping speeds for the nitrogen gas are measured under the inlet pressure range of $0.001{\sim}4$ Torr. The maximum of compression ratio was about 3300 for three-stage DTDP, 1000 for two-stage and 100 for single-stage DTDP at zero throughput. The number of stage influences the pumping speed of DPDT

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A Study on the Optimal Structural Design and Ultra Precision Position Control using FEM for Micro Stage (마이크로 스테이지의 유한요소법을 이용한 최적설계와 초정밀 위치제어에 관한 연구)

  • Kim, J. Y.;Han, J. H.;Kim, H. W.;You, S.;Kwac, L. K.;Song, I. S.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1997.10a
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    • pp.336-340
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    • 1997
  • For optimal design of micro stage, we were measured to displacement of piezo-electric transducer that was based on voltage value. And then researchers were analyzed to microstage through FEM with displacement data including voltage value of piezo-electric transducer. For verification of analyzing results, we were gauged on displacement by using Laser-interferometer. And researchers were confirmed to propriety of micro stage design with FEM, were obtained error rate that are 3.5% between measurement results and analyzing results.

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An Experimental Study on the Pumping Performance of the Two-Stage Disk-Type Drag Pump (2단 원판형 드래그펌프의 배기 성능에 관한 실험적 연구)

  • Hwang, Young-Kyu;Heo, Joong-Sik;Kwon, Myoung-Keun;Yang, Seong-Min
    • Proceedings of the KSME Conference
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    • 2001.11b
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    • pp.347-352
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    • 2001
  • In this paper, the pumping performance of the two-stage disk-type drag pump which works in the outlet pressure range from 3 to 0.001 Torr is studied experimentally. The rotational speed of the pump is 24,000rpm, and nitrogen is used as a test gas. The pumping characteristics of various drag pumps are performed. The inlet pressures are measured for various outlet pressures of the test pump. The maximum compression ratios for zero throughput are 1000(two-stage BSC type), 740(helical-type), 90(BSC type) and 85(OSC type), respectively. The ultimate pressure of the two-stage disk-type drag pump is $8.1\times10^6$ Torr.

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