• 환경생물
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• 제40권3호
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• pp.247-254
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• 2022

본 연구는 자연에서 채집한 불볼락(Sebastes thompsoni)을 대상으로 고 수온 노출에 따른 생물의 행동반응 및 내성 한계(tolerance limit)를 규명하기 위하여 연속적으로 행동관찰이 가능한 추적시스템을 사용하여 노출 수온구간별 행동패턴의 변화, 임계 수온범위 및 아치사 행동반응을 분석하였다. 실험 결과, 대조수온(15.0℃)을 20.0℃로 높였을 때, BI (behavioral index)는 노출 초기 10시간 동안 일시적으로 증가하였으나 60~70시간 동안 뚜렷한 행동의 변화 없이 일정한 속도로 안정된 유영 패턴을 보였다. 그러나, 25℃에서는 활동량의 급격한 저하, 30℃에서는 활동성 지연과 정지행동 등과 같은 비정상적 유영 및 대사활동 교란이 확인되었으며, 온도의 상승에 따라 개체의 사망률이 높아지는 경향을 보였다. 이러한 결과는, 불볼락의 수온에 대한 내성한계는 25.0~30.0℃이며 생존에 큰 영향을 미치는 수온은 30.0℃ 부근임을 나타낸다. 본 연구의 결과가 효과적인 불볼락의 양식을 위한 환경 조건 설정에 도움을 줄 것으로 기대한다.

• ALGAE
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• 제35권4호
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• pp.375-388
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• 2020

Ocean acidification and warming, identified as environmental concerns likely to be affected by climate change, are crucial determinants of algal growth. The ichthyotoxic raphidophytes Chattonella species are responsible for huge economic losses and environmental impact worldwide. In this study, we investigated the impact of CO2 on the thermal performance curves (TPCs) of Chattonella marina and Chattonella ovata grown under temperatures ranging from 13 to 34℃ under ambient pCO2 (350 μatm) and elevated pCO2 (950 μatm). TPCs were comparable between the species or even between pCO2 levels. With the exception of the critical thermal minimum (CTmin) for C. ovata, CTmin for C. marina and the thermal optimum (Topt) and critical thermal maximum (CTmax) for both species did not change with elevation of pCO2 levels. While CO2 enrichment increased the maximum photosynthetic rates (Pmax) up to 125% at the Totp of 30℃, specific growth rates were not significantly different under elevated pCO2 for the two species. Overall, C. ovata is likely to benefit from climate change, potentially widening its range of thermal tolerance limit in highly acidic waters and contributing to prolonged phenology of future phytoplankton assemblages in coastal waters.

• 한국물환경학회지
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• 제27권3호
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• pp.314-321
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• 2011

The potential effects of increased water temperature on fish assemblages and their habitats were studied in the streams of the Han River watershed when the water temperature was supposed to increase by $1^{\circ}C$, $2^{\circ}C$, and $3^{\circ}C$ in each sampling site. The percent changes in suitable habitats for each species and in species number within a site were determined, based on the estimated maximum thermal tolerances of 51 fish species whose habitats were classified into four reaches: upstream, up-/midstream, midstream, and mid-/downstream. The maximum thermal tolerance ranged between $25.0^{\circ}C$ and $31.0^{\circ}C$ and significantly increased as the habitat reaches move from upstream to mid-/downstream. With the increases in water temperature, the average suitable habitats for all 51 species were decreased by 31% ($+1^{\circ}C$), 46% ($+2^{\circ}C$), and 60% ($+3^{\circ}C$). The increased water temperature, however, did not induce significant differences in the changes in suitable habitats among four reaches within each level of temperature increase. The relative frequencies of the sampling sites classified into six levels according to the percent change in the number of species in each site were significantly different among three levels of water temperature increase, with relatively greater changes in the sites where 0~20% and 61~100% of species were affected.

• 대한기계학회논문집A
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• 제39권4호
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• pp.423-428
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• 2015

에어컨 실내기 조립 시 부품간 간섭이 발생하는 위치와 원인 분석을 위해, 3차원 공차해석을 수행하고 부품공차 및 조립 갭을 최적화했다. 민감도 분석을 통해 초기 설계에서 drain과 body 조립부의 불량발생 확률이 72.6%로 가장 높은 것을 확인했고, 또한 유한요소해석(FEA)을 활용 에어컨 실내기의 난방작동 시 열 변형으로 인해 최대 약 1mm의 변위가 발생하여 부품간 간섭이 발생할 수 있음을 확인했다. 이런 부품간 간섭을 최소화기 위해서 3D 공차 해석의 민감도 분석결과를 토대로 drain의 부품공차를 수정하였다. 개선 공차 반영 해석결과 불량률이 0.03%로 크게 감소함을 확인하였다. 또한, 개선공차 반영 시 에어컨 작동 중 열 변형이 발생하여도 부품간 간섭이 발생하지 않음을 FEA를 통해 검증하였다.

• 설비공학논문집
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• 제20권8호
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• pp.534-540
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• 2008

In this paper, the optimal bang-bang control problem of an air-conditioning system with slab thermal storage was formulated by gradient method. Furthermore, the numeric solution obtained by gradient method algorithm was compared with the analytic solution obtained on the basis of maximum principle. The control variable is changed uncontinuously at the start time of thermal storage operation in an analytic solution. On the other hand, it is showed as a continuous solution in a numeric solution. The numeric solution reproduces the analytic solution when a tolerance for convergence is applied severely. It is conceivable that gradient method is effective in the analysis of the optimal bang-bang control of the large-scale system like an air-conditioning system with slab thermal storage.

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1119-1125
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• 2024

Phytase increases the availability of phosphate and trace elements by hydrolyzing the phosphomonoester bond in phytate present in animal feed. It is also an important enzyme from an environmental perspective because it not only promotes the growth of livestocks but also prevents phosphorus contamination released into the environment. Here we present a novel phytase derived from Turicimonas muris, TmPhy, which has distinctive structure and properties compared to other previously known phytases. TmPhy gene expressed in the Pichia system was confirmed to be 41 kDa in size and was used in purified form to evaluate optimal conditions for maximum activity. TmPhy has a dual optimum pH at pH3 and pH6.8 and exhibited the highest activity at 70℃. However, the heat tolerance of the wildtype was not satisfactory for feed application. Therefore, random mutation, disulfide bond introduction, and N-terminal mutation were performed to improve the thermostability of the TmPhy. Random mutation resulted in TmPhyM with about 45% improvement in stability at 60℃. Through further improvements, a total of three mutants were screened and their heat tolerance was evaluated. As a result, we obtained TmPhyMD1 with 46.5% residual activity, TmPhyMD2 with 74.1%, and TmPhyMD3 with 66.8% at 80℃ heat treatment without significant loss of or with increased activity.

• 한국해양생명과학회지
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• 제2권2호
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• pp.83-89
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• 2017

In abalones, interspecific hybridization has been suggested as a possible means to increase production and desired traits for the industry. In Korea, Haliotis gigantea is considered a species with a larger size and higher temperature tolerance than H. discus hannai. However, H. discus hannai is considered the most valuable and popular fishery resource due to its better acceptance and higher market prices. Thus, viable interspecific hybrids have been produced by artificial inseminating H. gigantea eggs with H. discus hannai sperm. However, the reciprocal hybrid cross was not successful. In this study, the hybridity and the growth and thermal tolerance performance of the interspecific hybrids were examined. A combination of various assays revealed maximum growth occurrence at 21℃ and the higher growth rate in the hybrids than that of H. discus hannai parent. In addition, the growth and survival at high-temperature (28℃) of the hybrids was equivalent to that of the highly tolerant H. gigantea parent, suggesting new possibilities to overcome the mass mortality in H. discus hannai during high temperature periods of summer season in Korea. Furthermore, the induced interspecific hybrid status was confirmed by the presence of species-specific bands for each parental species of the random amplified polymorphic DNA (RAPD) profiles using universal rice primer (URP), which could be used as speciesspecific markers to distinguish the hybrids and their parental species.

• Biomolecules & Therapeutics
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• 제5권2호
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• pp.150-157
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• 1997

Capsaicin cream has been used to attenuate the pain associated with diabetic neuropathy, rheum-atoid arthritis, osteoarthritis and postherpetic neuralgia. But its common side effect, local irritation, limits the use of it and there is still a need for a new analgesic devoid of this side effect. This study was conducted to compare the local irritant effect of DA-5018, a new capsaicin derivative, with that of capsaicin in various animal models and human beings. Capsaicin, applied topically to the mouse ear, produced dose-dependent increase of ear volume and the frequency of ear scratching behavior in mice. Neither ear volume nor scratching behavior was affected by DA-5018. In eye wiping test of rat, DA-5018 was 10 times less irritant than capsaicin. Capsaicin administered intradermally into the rat paw elicited paw lick/lift response with a potency which was three times that of DA-5018. Zostrix-HP (0.075% capsaicin cream), but not DA-50180.3% cream, increased ear volume of rat and induced thermal hyperalgesia in normal and carrageenan inflamed paws. Six day-treatment of Zostrix-HP failed to develop tolerance against this thermal hyperalgesia. In human beings, Zostrix-HP produced burning sensation and itching in more than 90% of volunteers involved and its maximum irritant effect was significantly higher than that of DA-5018 cream. These results suggest that local irritation and burning sensation produced by DA-5018 is much less than capsaicin.

• 한국수자원학회논문집
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• 제46권1호
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• pp.1-12
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• 2013

본 연구에서는 기후변화에 따른 수온상승이 낙동강 유역 어류 서식지에 미치는 영향을 분석하였다. 이를 위해 낙동강유역에 서식하는 어종 중 22개 어종에 대한 최대내성한계온도를 산출하였으며, 이를 토대로 수온 상승에 따른 어류 서식지 및 서식지 지점별 어종 영향 정도를 분석하였다. 산정된 최대내성한계온도의 범위는 $27.7\sim33.1^{\circ}C$의 범위를 나타났다. 또한 기존에 예측된 기온 상승 데이터를 이용하여, 낙동강 78개 지점에서의 2100년도까지의 수온 상승을 예측하였으며, 수온 상승 예측을 위하여 낙동강 유역 78개 각 지점에서의 기온-수온의 회귀식을 산정하였다. 그 결과 S1 기간(2011~2040년)에 평균 $0.69^{\circ}C$, S2기간(2041~2070년)에는 평균 $1.76^{\circ}C$, S3기간(2071~2100년)에는 평균 $2.32^{\circ}C$의 수온이 상승되는 것으로 나타났다. 이상의 수온상승에 따라 낙동강 22개 어종에 대해 S1-S3 각 기간별로 평균 21.9%, 36.3%, 51.4%의 서식처가 영향을 받는 것으로 나타났다.

• 한국농림기상학회지
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• 제14권1호
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• pp.11-18
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• 2012

월별 기후통계량의 조화해석에 의해 생성한 일 기온 자료가 생물계절모형의 입력자료로서 적합한지 여부를 평가하여 농림업 부문 기후시나리오 응용정보 제작 상오류를 제거하기 위해 본 연구를 수행하였다. 서울관측소의 1971-2000 평년 월별 일 최고기온과 최저기온 평균값으로부터 조화해석에 의해 365일 간 기온자료를 생성하였다. 이것을 널리 검증된 온도시간 기반의 벚꽃 개화모형에 입력하여 휴면, 발아, 개화 등 주요 식물계절을 추정하였다. 같은 기간 중 실측기온자료에 의해 모형을 구동시켜 얻은 결과와 비교한 바, 연차변이를 전혀 반영하지 못하는 것은 물론, 휴면해제 25일 단축, 강제 휴면기간 57일 연장, 발아 14일 지연, 개화 13일 지연등 평균값도 크게 달라 식물계절을 크게 왜곡시키는 것으로 판단되었다. 대안으로서 확률추정기법에 의해 일기상자료를 생성하고 이를 이용하여 모형을 구동한 결과 실측결과에 비해 휴면해제 6일 단축, 강제휴면기간 10일 단축, 발아 3일 지연, 개화 2일 지연 등으로 조화해석자료 사용에 비해 크게 개선되었음을 확인하였다. 연차변이양상 역시 실측기온에 의한 모의결과와 크게 다르지 않아, 향후 이 자료를 농업부문 전자기후도 제작에 적용하면 기후변화 적응정책 수립을 실용수준에서 지원할 수 있을 것으로 보인다.