• 대한환경공학회지
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• 제39권6호
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• pp.332-338
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• 2017

본 연구는 혼합영양생물인 Scenedesmus acuminatus의 최적배양조건을 찾기 위한 연구로서 최적 공기주입량, 아세테이트 주입량, $CO_2$ 주입에 대해 실험을 진행하였다. 공기주입량은 0.72 vvm에서 최대 비성장속도와 최대바이오매스 생산량을 얻을 수 있었으며, 0.3 M의 아세테이트를 주입하였을 때 최대 비성장속도와 최대바이오매스 생산량을 얻었다. 반연속배양에서는 추가로 $CO_2$ 주입이 배양에 미치는 영향을 파악하였다. $CO_2$ 주입 실험에서는 아세테이트로 50% 치환한 후에 최대비성장속도($0.460d^{-1}$)와 최대바이오매스 생산성($0.936gL^{-1}d^{-1}$)을 얻을 수 있었으며 이후 치환이 계속될수록 최대비성장속도와 최대바이오매스 생산성은 지속적으로 감소하였다. 공기주입 실험에서는 JM 배지로 배양할 때 가장 높은 비성장속도($0.381d^{-1}$)와 최대바이오매스 생산성($0.253gL^{-1}d^{-1}$)을 보여주었지만 이후 아세테이트를 함유한 배지로 50% 치환하였을 때 오히려 초기값보다 감소하는 것을 관찰하였다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1979년도 춘계학술대회
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• pp.115.2-115
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• 1979

By employing a two-stage continuous culture system, some of important physiological parameters involved in cellulase bicsynthesis have been evalua-ted with an ultimate objective of detigning an op-timally controlled tellulase process. Volumetric and specific cellulase productivities obtained were 90 IU/liter/hr and 8IU/g biomass/hr respectively. The maximum specific enzyme productivity observed was 14.8 IU/g hiomass/hr. The optimal dilution rate in the second stage which corresponded to the maximum enzyme productivity was 0.026-0.028 hr$^{-1}$ , and the specific growth rate in the second stage ihat suported maximum specific enzyme productivity was equal to orslightly less than zero. The maintenance coefficients deter-mined for oxygen and for carbon source are M$_{o}$=0.85mmmole/g biomass/hr and M$_{c}$=0.14 mmole hexose/g bio mass/hr respectively. The yield constants determined are; Y(x/o) =32.3g biomass/mole oxygen, Y (x/c) =1.1g bio-mass/g carbon or 0.44g biomass/g hexose, Y(x/n) = 19.6g biomass/g nitrogen for the enzyme produc-tion stage and 12.5g biomass/g nitrogen for the cell growth stage.e.e.

• 대한환경공학회지
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• 제27권4호
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• pp.409-413
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• 2005

본 논문에는 자가영양균의 최대비성장율 추정법이 제시되었다. 먼저 질산화균의 농도가 질산화 된 암모니아, 슬러지 일령 및 사멸계수를 이용하여 시뮬레이션 되었고, 다음단계로 과잉암모니아를 공급하여 질산화균의 호흡율을 측정하였다. 자가영양균의 최대비성장율은 ${\mu}_{max,A}\;=\;OUR_{max,A}/Y_A$의 관계를 통해 계산되어질 수 있으며 추정된 최대비성장율은 운전기간에 걸쳐 일정한 값을 가지지 앉고 시간에 따라 변화한다는 결과를 얻었다. 본 연구를 통해 최대호흡율을 이용한 최대비성장율의 동적 추정법이 수행되었고, 일정한 최대비성장율를 이용한 처리장 운전결과 예측은 처리장 거동을 예측할 수 없으며 활성슬러지공정의 성능예측을 위한 시뮬레이션을 위해서는 동적 추정된 매개변수의 사용이 필요함을 확인하였다.

• 상하수도학회지
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• 제33권1호
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• pp.55-62
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• 2019

The respirometric technique has been used to analyze the nitrification process in a sequencing batch reactor(SBR) treating municipal wastewater. Especially the profile of the respiration rate very well expressed the reaction characteristics of nitrification. As the nitrification process required a significant amount of oxygen for nitrogen oxidation, the respiration rate due to nitrification was high. The maximum nitrification respiration rate, which was about $50mg\;O_2/L{\cdot}h$ under the period of sufficient nitrification, was related directly to the nitrification reaction rate and showed the nitrifiers activity. The growth rate of nitrifiers is the most critical parameter in the design of the biological nutrient removal systems. On the basis of nitrification kinetics, the maximum specific growth rate of nitrifiers in the SBR was estimated as $0.91d^{-1}$ at $20^{\circ}C$, and the active biomass of nitrifiers was calculated as 23 mg VSS/L and it was about 2% of total biomass.

• Korean Chemical Engineering Research
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• 제56권1호
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• pp.73-78
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• 2018

본 연구는 혼합영양 배양 조건에서 acetate의 주입이 Anabena azollae의 성장에 미치는 영향을 파악하기 위해 수행되었다. 4가지 종류의 acetate 중 ethyle acetate가 Anabena azollae의 성장에 가장 효과적이라고 밝혀졌으며, 주입한 ethyle acetate의 농도가 증가할수록 성장속도는 증가하는 것을 확인하였다. 40 mM의 ethyl acetate의 경우 비성장속도는 $0.979day^{-1}$, 최대바이오매스 생산성은 $0.293g\;L^{-1}\;d^{-1}$로 본 연구에서 배양속도가 가장 빠른 것으로 판명되었다. Acetic acid와 butyl acetate의 경우 Anabena azollae 성장을 방해하는 것으로 나타났다. Aetration의 경우 0.54 vvm에서 성장속도가 가장 빨랐다. 반연속배양에서는 aeration 실험이 끝난 후 연속하여 ethyle acetate 주입을 하여 배양을 하였다. 회분식실험에 비해 반연속배양에서의 비성장속도와 최대바이오매스 생산성은 모두 감소하였지만 최대 농도는 5.91 g/L로 가장 큰 값을 나타내었다.

• Environmental Engineering Research
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• 제10권6호
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• pp.283-293
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• 2005

Characteristics of sulfur-based autotrophic denitrification in a semi-continuous type reactor and the kinetic parameters were studied. Enriched autotrophic denitrifying culture was used for the reactor operation. Biomass growth on sulfur particles and in the liquid medium was monitored using the DAPI staining method. From the result of ion concentration changes and the biomass growth, maximum specific growth rate, ${\mu}_{max}$, and the half velocity constant, $K_M$, were estimated as $0.61\;d^{-1}$ and 3.66 mg/L, respectively. Growth yield coefficient, Y values for electron acceptor and donor were found as 0.49 gVSS/g N and 0.16 gVSS/g S. The biomass showed specific denitrification rate, ranging 0.86-1.13 gN/g VSS-d. A half-order equation was found to best simulate the denitrification process in the packed bed reactor operated in the semi-continuous mode.

• 한국환경과학회지
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• 제19권5호
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• pp.647-653
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• 2010

The objectives of this research are to evaluate and compare the oxygen transfer coefficients($K_{La}$) in both a general bubbles reactor and a micro-nano bubbles reactor for effective operation in sewage treatment plants, and to understand the effect on microbial kinetic parameters of biomass growth for optimal biological treatment in sewage treatment plants when the micro-nano bubbles reactor is applied. Oxygen transfer coefficients($K_{La}$) of tap water and effluent of primary clarifier were determined. The oxygen transfer coefficients of the tap water for the general bubbles reactor and micro-nano bubbles reactor were found to be 0.28 $hr^{-1}$ and 2.50 $hr^{-1}$, respectively. The oxygen transfer coefficients of the effluent of the primary clarifier for the general bubbles reactor and micro-nano bubbles reactor were found be to 0.15 $hr^{-1}$ and 0.91 $hr^{-1}$, respectively. In order to figure out kinetic parameters of biomass growth for the general bubbles reactor and micro-nano bubbles reactor, oxygen uptake rates(OURs) in the saturated effluent of the primary clarifier were measured with the general bubbles reactor and micro-nano bubbles reactor. The OURs of in the saturated effluent of the primary clarifier with the general bubbles reactor and micro-nano bubbles reactor were 0.0294 mg $O_2/L{\cdot}hr$ and 0.0465 mg $O_2/L{\cdot}hr$, respectively. The higher micro-nano bubbles reactor's oxygen transfer coefficient increases the OURs. In addition, the maximum readily biodegradable substrate utilization rates($K_{ms}$) for the general bubbles reactor and micro-nano bubbles reactor were 3.41 mg COD utilized/mg active VSS day and 7.07 mg COD utilized/mg active VSS day, respectively. The maximum specific biomass growth rates for heterotrophic biomass(${\mu}_{max}$) were calculated by both values of yield for heterotrophic biomass($Y_H$) and the maximum readily biodegradable substrate utilization rates($K_{ms}$). The values of ${\mu}_{max}$ for the general bubbles reactor and micro-nano bubbles reactor were 1.62 $day^{-1}$ and 3.36 $day^{-1}$, respectively. The reported results show that the micro-nano bubbles reactor increased air-liquid contact area. This method could remove dissolved organic matters and nutrients efficiently and effectively.

• 미생물학회지
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• 제20권2호
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• pp.67-72
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• 1982

The effect of fermentation temperature on the production of high content alcohol has been investigated with high substrate concentration. The maximum specific growth rate, ${\mu}max\;was\;0.461hr^{-1}\;at\;35^{\circ}C$ which was the highest, whereas the maximum biomass concentration waas 8.7g/l at $25^{\circ}C$, at the growth rate lower than at $35^{\circ}C$. Approximately 140g/l of ethanol was produced in the temperature range of 20 to $25^{\circ}C$ with nearly complete comsumption of the substrate. Extended fermentation time has been required at lower temperatures, however, for the maximum values of biomass concentration and alcohol content, hence higher ethanol productivity, as the temperature was elevated to $40^{\circ}C$. The viability of yeasts was greatly improved by lowering the fermentation temperature down to $25^{\circ}C$ and also extended survival of the cells has been observed at lower fermentation temperatures, although the ethanol concentration of both waas higher.

• 한국미생물·생명공학회지
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• 제25권3호
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• pp.235-239
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• 1997

Chlorococcum littorale has been grown in high $CO_2$ concentrations to utilize $CO_2$ gas in the polluted air. The effect of incident light intensity on the specific growth rate is expressed by a photoinhibition model, showing half- saturation constant, $K_0\;as\;8\;(W/m^2)$ and inhibition constant, Ki as 35 $(W/m^2)$. The maximum specific growth rate was also estimated as 0.095 (1/day) under this condition. This strain maintained the optimum growth rate in 20% of $CO_2$ gas but 50% of input $CO_2$ gas is the maximum concentration considering the economical efficiency. The maximum Specific $CO_2$ consumption rate, $qCO_2$ was measured as 17.48 (mg $CO_2/g$ dry wt./day) in batch cultivation, 11.2 (mg $CO_2/g$ dry wt./day) in fed-batch cultivation and 10.87 (mg $CO_2/g$ dry wt./day) at 0.065 (1/day) of dilution rate in continuous cultivation. The chemical composition of the biomass obtained from this process showed 32.5% of protein, 27.5% of lipid, 16.5% of carbohydrate and ash 11.7%.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권1호
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• pp.43-47
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• 2006

Ginseng (Panax ginseng CA. Meyer) hairy root cultures, which are established via the infection of ginseng root discs with Rhizobium rhizogenes, have been used to construct profiles of both biomass growth and nutrient consumption in flask cultures. In a 250 mL shake flask culture, the maximum biomass was observed on the 59th day of the culture period, at 216.8 g (fresh wt) per liter or 11.4 g (dry wt) per liter. The hairy roots were determined to have a growth rate of 0.355 g-DW/g cells/day during the exponential growth phase and a maximum specific growth rate on day 7. Total ginseng saponin and phenolic compound contents were noted to have increased within the latter portion of the culture period. Linear correlations between increases in biomass weight and nutrient uptake were used to imply the conductivity yield $2.60g-DW/(L{\cdot}mS)$ and carbon yield 0.45 g-DW/(g sugar) in the 250 mL flask cultures. The biomass yield when two different nitrogen sources were used (ammonia and nitrate) was shown to remain approximately constant. at $0.47g-DW/(L{\cdot}mM\;NH_4$) and $0.33g-DW/(L{\cdot}mM\;NO_3$); it remained at these levels for 16 days with the ammonia. and for 24 days with the nitrate. The biomass yield when a phosphate source was used was also shown to remain approximately constant for 9 days, at $3.17g-DW/(L{\cdot}mM\;PO_4$), with an $R^2$ of 0.99.